图/表 详细信息

基于Bt毒素的杀虫蛋白理性设计与创新应用策略
徐重新, 金嘉凤, 孙晓明, 沈成, 张霄, 陈澄宇, 刘贤金, 刘媛
中国农业科学, 2024, 57(1): 96-125.   DOI: 10.3864/j.issn.0578-1752.2024.01.008

供试毒素
Tested toxin
增效物
Synergist
实施效果
Implementation effect
参考文献
Reference
Cry1Ab/
Cry1Ac
活化增效物Activating synergist
M. sexta cadherin repeats (CR)(-)对烟草天蛾的致死率(>85%)较Cry1Ab(<10%)、Cry1Ac(<15%)单独使用提高8.50、5.66倍以上Its lethality rate to M. sexta (>85%) was above 8.50-, 5.66-fold higher than that of the Cry1Ab (<10%), Cry1Ac (<15%) used alone[111]
Cry1Ac/
Cry1Ca
S. exigua cadherin-like protein (rSeCad1bp)(-)对甜菜夜蛾和棉铃虫的生长发育均显著抑制(体重减少25%—52%)The growth and development of S. exigua and H. armigera (body weight loss of 25%-52%) were significantly inhibited[112]
Cry1AcH. armigera cadherin fragment (HaCad1)(-)对棉铃虫的致死率(>80%)较Cry 1Ac(<15%)单独使用提高5.3倍以上Its lethality rate to H. armigera (>80%) was above 5.3-fold higher than that of the Cry1Ac (<15%) used alone[16]
Cry1B/
Cry1C
S. exigua cadherin-like protein (CR11-MPED)(-)对甜菜夜蛾的致死率(38.87%)较Cry1B(24.43%)、Cry1C(15.57%)单独使用提高1.59、2.50倍Its lethality rate to S. exigua (38.87%) was 1.59-, 2.50-fold higher than that of the Cry1B (24.43%), Cry1C (15.57%) used alone[17]
Cry1FaM. sexta cadherin (MsCad), S. frugiperda cadherin (SfCad)(-)对草地贪夜蛾的致死毒力分别提高1.8倍(MsCad)和5.2倍(SfCad)Its lethal virulence to S. frugiperda was improved by 1.8-fold (MsCad) and 5.2-fold (SfCad), respectively[148]
Cry3Aa/
Cry3Bb
D. virgifera cadherin repeats (CR)(-)对马铃薯甲虫、黄瓜甲虫和玉米根虫的致死毒力提高3—13倍(数据多未细列)Its lethal virulence to L. decemlineata, Diabrotica undecimpunctata and D. virgifera was improved by 3-13 folds (detailed data not shown)[149]
Cry1AcM. sexta aminopeptidase(-), phosphatase(-)对烟草天蛾幼虫肠壁细胞损害程度显著增强(原文未显示数值)Its damage degree to the intestinal wall cells of M. sexta larvae was significantly enhanced (detailed data not shown in article)[150]
Cry8Hb/
Cry3-type
Escherichia coli maltose binding protein (MBP)(-)对玉米根虫(EC50为16.1 ng·mL-1)的致死毒力较Cry8Hb/Cry3-type(EC50为214 ng·mL-1)单独使用提高13.3倍Its lethal virulence to D. virgifera (EC50 was 16.1 ng·mL-1) was 13.3-fold higher than that of the Cry8Hb/Cry3-type (EC50 was 214 ng·mL-1) used alone[113]
Cry2Aa靶向受体结合增效物Targeted receptor binding synergistB. thuringiensis P20 helper protein(-)对地中海粉螟、尖音库蚊和埃及伊蚊的致死毒力较对照组Cry2Aa分别提高1.43、341.28和354.62倍(数据多未细列)Its lethal virulence to Ephestia kuehniella, C. pipiens and A. aegypti was 1.43-, 341.28- and 354.62-fold higher than that of the Cry2Aa control (detailed data not shown)[19]
Cry1Ab/
Cry1Ac
P. xylostella Hsp90 (PxHsp90) chaperone protein(-)对小菜蛾和草地贪夜蛾的致死率提高4—8倍(数据多未细列)Its lethality rate to P. xylostella and S. frugiperda was improved by 4-8 folds (detailed data not shown)[18]
Cry1AcBacillus enhancin-like protein (Bel)(-)对棉铃虫致死率(74.4%)较Cry1Ac(34.2%)单独使用提高2.18倍Its lethality rate to H. armigera (74.4%) was 2.18-fold higher than that of the Cry1Ac (34.2%) used alone[114]
Cry2Aa表达增效物Expression synergistB. thuringiensis P20 helper protein(-)促进Cry2Aa在苏云金芽孢杆菌中的表达量提高7倍The protein expression of Cry2Aa in B. thuringiensis increased by 7-fold[19]
Cry10A/
Cyt1C
B. thuringiensis QBT220-pBtoxis plasmid(-)Cry10A/Cyt1C蛋白表达量提高1.29倍,对埃及伊蚊的致死毒力同步增强1.5倍The protein expression of Cry10A/Cyt1C increased by 1.29-fold, and the lethal virulence to A. aegypti increased by 1.5-fold[115]
Vip3AaB. thuringiensis strong promoter (Prsi)(-)+ Protease gene knocked out B. thuringiensis strains (BMB171)(-)Vip3Aa蛋白表达量显著提高,且对甜菜夜蛾(LC50为13.05 μL·g-1)的致死毒力较对照(LC50为59.72 μL·g-1)提高4.58倍The protein expression of Vip3Aa increased significantly, and the lethal virulence to S. exigua (LC50 was 13.05 μL·g-1) was 4.58-fold than that of the control (LC50 was 59.72 μL·g-1)[116]
Cry1AcCotton leaf curl Kokhran virus-Burewala strong promoter (CLCuKoV-Bu)(-)转基因棉中Cry1Ac蛋白表达量提升3倍,间接增强转基因作物对靶标害虫的防控能力The protein expression of Cry1Ac in transgenic cotton increased by 3-fold, which indirectly enhanced the ability of transgenic crops to control target pests[117]
Cry1AhMaize ubi1 intron(-)转基因玉米中Cry1Ah蛋白表达量提高20%,间接增强转基因作物对靶标害虫的防控能力The protein expression of Cry1Ah in transgenic maize increased by 20%, which indirectly enhanced the ability of transgenic crops to control target pests[118]
Cry1Ab/
Cry1Ac
Azotobacter(-)促进Cry1Ab/Cry1Ac蛋白表达,对棉铃虫的防控效果显著提高(原文未显示数值)It promoted the protein expression of Cry1Ab/Cry1Ac, and the control effect to H. armigera was significantly improved (detailed data not shown in article)[119]
Cry1AcAmino acids(-)转基因棉中Cry1Ac蛋白表达量提高15.2%—25.8%,间接增强转基因作物对靶标害虫的防控能力The protein expression of Cry1Ac in transgenic cotton increased by 15.2%-25.8%, which indirectly enhanced the ability of transgenic crops to control target pests[120]
Cry11AaLate embryogenesis abundant peptide (LEA-II)(-)促进Cry11Aa蛋白表达量提升3倍,间接增强表达制剂对靶标害虫的防控能力The protein expression of Cry11Aa increased by 3-fold, which indirectly enhanced the ability of expression products to control target pests[20]
Cry1C同源协同增效物Homology cooperative synergistCry1Aa(+)对甜菜夜蛾(LC50为0.537 mg·g-1)的致死毒力较Cry1C(LC50为1.466 mg·g-1)和Cry1Aa(LC50为3.936 mg·g-1)单独使用提高2.73和7.33倍Its lethal virulence to S. exigua (LC50为0.537 mg·g-1) was 2.73- and 73.3-fold higher than that of the Cry1C (LC50 was 1.466 mg·g-1) and Cry1Aa (LC50 was 3.936 mg·g-1) used alone[151]
Cry3ABt spores(+)对马铃薯甲虫的致死毒力较两者单独使用提高2.6倍(Cry3A)和4.9倍(Bt spores)Its lethal virulence to L. decemlineata was 2.6- and 4.9-fold higher than that of the Cry3A and Bt spores used alone[152]
Cry4AaCyt2Ba(+)对埃及伊蚊(LC50为13.41 ng·mL-1)的致死毒力较Cry4Aa(LC50为34.63 ng·mL-1)和Cyt2Ba(LC50为279.37 ng·mL-1)单独使用提高2.58和20.83倍Its lethal virulence to A. aegypti (LC50 was 13.41 ng·mL-1) was 2.58- and 20.83-fold higher than that of the Cry4Aa (LC50 was 34.63 ng·mL-1) and Cyt2Ba (LC50 was 279.37 ng·mL-1) used alone[128]
Cry4BaCyt2Aa(+)对埃及伊蚊(LC50为7 ng·mL-1)和致倦库蚊(LC50为20 ng·mL-1)的致死毒力较Cry4Ba (LC50分别为140 ng·mL-1和无活性)和Cyt2Aa(LC50分别为350和250 ng·mL-1)单独使用提高12.5—50倍Its lethal virulence to A. aegypti (LC50 was 7 ng·mL-1) and C. quinquefasciatus (LC50 was 20 ng·mL-1) was 12.5-50 folds higher than that of the Cry4Ba (LC50 was 140 ng·mL-1 and inactive) and Cyt2Aa (LC50 was 350 and 250 ng·mL-1) used alone[21]
Cry9AaVip3Aa(+)对二化螟(LC50为0.13 μg·g-1)的致死毒力较Cry9Aa(LC50为0.70 μg·g-1)和Vip3Aa(LC50为37.41 μg·g-1)单独使用提高5.38和287.76倍Its lethal virulence to C. suppressalis (LC50 was 0.13 μg·g-1) was 5.38- and 287.76-fold higher than that of the Cry9Aa (LC50 was 0.70 μg·g-1) and Vip3Aa (LC50 was 37.41 μg·g-1) used alone[131]
Cry9EeVip3Aa(+)对二化螟(LC50为1.78 μg·g-1)的致死毒力较Cry9Ee(LC50为1.96 μg·g-1)和Vip3Aa(LC50为27.77 μg·g-1)单独使用提高1.1和15.6倍Its lethal virulence to C. suppressalis (LC50 was 1.78 μg·g-1) was 1.1- and 15.6-fold higher than that of the Cry9Ee (LC50 was 1.96 μg·g-1) and Vip3Aa (LC50 was 27.77 μg·g-1) used alone[132]
Cry10AaCyt2Ba(+)对埃及伊蚊(LC50为4.22 ng·mL-1)的致死毒力较Cry10Aa(LC50为299.62 ng·mL-1)和Cyt2Ba(LC50为279.37 ng·mL-1)单独使用提高71和66.2倍Its lethal virulence to A. aegypti (LC50 was 4.22 ng·mL-1) was 71- and 66.2-fold higher than that of the Cry10Aa (LC50 was 299.62 ng·mL-1) and Cyt2Ba (LC50 was 279.37 ng·mL-1) used alone[128]
Cry11AaCyt1Aa(+)对白纹伊蚊(LC50为17.1 ng·mL-1)的致死毒力较Cry11Aa(LC50为228 ng·mL-1)和Cyt1Aa(LC50为171 ng·mL-1)单独使用提高13.3和10倍Its lethal virulence to A. albopictus (LC50 was 17.1 ng·mL-1) was 13.3- and 10-fold higher than that of the Cry11Aa (LC50 was 228 ng·mL-1) and Cyt1Aa (LC50 was 171 ng·mL-1) used alone[129]
Cry11BaCry4Aa(+)对尖音库蚊(LC50为0.04 μg·mL-1)的致死毒力较Cry11Ba(LC50为0.11 μg·mL-1)和Cry4Aa(LC50为0.97 μg·mL-1)单独使用提高2.75和24.25倍Its lethal virulence to C. pipiens (LC50 was 0.04 μg·mL-1) was 2.75- and 24.25-fold higher than that of the Cry11Ba (LC50 was 0.11 μg·mL-1) and Cry4Aa (LC50 was 0.97 μg·mL-1) used alone[130]
Cry21FaCry21Ha(+)对秀丽隐杆线虫(LC50为6.1 μg·mL-1)的致死毒力较Cry21Fa(LC50为13.6 μg·mL-1)和Cry21Ha(LC50为23.9 μg·mL-1)单独使用提高2.23和3.92倍Its lethal virulence to C. elegans (LC50为6.1 μg·mL-1) was 2.23- and 3.92-fold higher than that of the Cry21Fa (LC50 was 13.6 μg·mL-1) and Cry21Ha (LC50 was 23.9 μg·mL-1) used alone[153]
Cry64BaCry64Ca(+)对灰飞虱(LC50为3.15 μg·mL-1)和白背飞虱(LC50为2.14 μg·mL-1)的致死毒力显著增强(原文未显示可比对数值)Its lethal virulence to Laodelphax striatellus (LC50 was 3.15 μg·mL-1) and Sogatella furcifera (LC50 was 2.14 μg·mL-1) was significantly enhanced (detailed comparable data not shown in article)[33]
Cyt1AaCry1Ca(+)对埃及伊蚊(LC50为0.61 μg·mL-1)的致死毒力较Cyt1Aa(LC50为0.73 μg·mL-1)和Cry1Ca(LC50为4.61 μg·mL-1)单独使用提高1.2和7.56倍Its lethal virulence to A. aegypti (LC50 was 0.61 μg·mL-1) was 1.2- and 7.56-fold higher than that of the Cyt1Aa (LC50 was 0.73 μg·mL-1) and Cry1Ca (LC50 was 4.61 μg·mL-1) used alone[127]
Cyt1AaCry2Aa(+)对致倦库蚊(LC50为15.2 μg·mL-1)的致死毒力较Cyt1Aa(LC50为31.3 μg·mL-1)和Cry2Aa(LC50>200 μg·mL-1)单独使用提高2.05倍和超过13倍Its lethal virulence to C. quinquefasciatus (LC50为15.2 μg·mL-1) was 2.05-fold and above 13-fold higher than that of the Cyt1Aa (LC50 was 31.3 μg·mL-1) and Cry2Aa (LC50>200 μg·mL-1) used alone[122]
Cyt1AaCry4Ba(+)对埃及伊蚊(LC50为66.7 ng·mL-1)的致死毒力较Cyt1Aa(LC50为952.5 ng·mL-1)和Cry4Ba(LC50为79.4 ng·mL-1)单独使用提高14.28和1.19倍Its lethal virulence to A. aegypti (LC50 was 66.7 ng·mL-1) was 14.28- and 1.19-fold higher than that of the Cyt1Aa (LC50 was 952.5 ng·mL-1) and Cry4Ba (LC50 was 79.4 ng·mL-1) used alone[63]
Vip3AaCry1F (+)对东方黏虫(LC50为1.8 μg·g-1)的致死毒力较Vip3Aa(LC50为7.4 μg·g-1)和Cry1F(LC50为14.4 μg·g-1)单独使用提高4.1和8倍 Its lethal virulence to Mythimna separata (LC50 was 1.8 μg·g-1) was 4.1- and 8-fold higher than that of the Vip3Aa (LC50 was 7.4 μg·g-1) and Cry1F (LC50 was 14.4 μg·g-1) used alone[133]
Vip3AaCry1Ie (+)对东方黏虫(LC50为2.0 μg·g-1)的致死毒力较Vip3Aa(LC50为7.4 μg·g-1)和Cry1Ie(LC50为78.6 μg·g-1)单独使用提高3.7和39.3倍Its lethal virulence to M. separata (LC50 was 2.0 μg·g-1) was 3.7- and 39.3-fold higher than that of the Vip3Aa (LC50 was 7.4 μg·g-1) and Cry1Ie (LC50 was 78.6 μg·g-1) used alone[133]
Cry11AaCyt1A-like δ endotoxins(+)对白纹伊蚊(LC50为19.7 ng·mL-1)的致死毒力较Cry11Aa(LC50为228 ng·mL-1)和Cyt1A-like(LC50>105 ng·mL-1)单独使用提高11.5和超过5000倍Its lethal virulence to A. albopictus (LC50 was 19.7 ng·mL-1) was 11.5-fold and above 5000-fold higher than that of the Cry11Aa (LC50 was 228 ng·mL-1) and Cyt1A-like (LC50>105 ng·mL-1) used alone[129]
Cry11AaCry-like δ endotoxins (Mtx-1)(+)对致倦库蚊(LC95为1.93 μg·mL-1)的致死毒力较Cry11Aa(LC95为33.5 μg·mL-1)和Mtx-1(LC95为2.4 μg·mL-1)单独使用提高17.36和1.24倍Its lethal virulence to C. quinquefasciatus (LC95 was 1.93 μg·mL-1) was 17.36- and 1.24-fold higher than that of the Cry11Aa (LC95 was 33.5 μg·mL-1) and Mtx-1 (LC95 was 2.4 μg·mL-1) used alone[154]
Cyt1AaL. sphaericus binary toxin (BinA)(+)对Bt耐药性致倦库蚊(LC50为10.605 μg·mL-1)的致死毒力较Cyt1Aa(LC50>50 μg·mL-1)和BinA(极低活性)单独使用显著提高Its lethal virulence to Bt-resistance C. quinquefasciatus (LC50 was 10.605 μg·mL-1) was significantly enhanced than that of the Cyt1Aa (LC50>50 μg·mL-1) and BinA (very low activity) used alone[134]
Cry5Ba异源协同增效物Heterology cooperative synergistB. thuringiensis metalloproteinase (Bmp1)(+)对秀丽隐杆线虫(LC50为37.5 μg·mL-1)的致死毒力较Cry5Ba(LC50为226.67 μg·mL-1)和Bmp1(LC50为610 μg·mL-1)单独使用提高6.04和16.27倍Its lethal virulence to C. elegans (LC50 was 37.5 μg·mL-1) was 6.04- and 16.27-fold higher than that of the Cry5Ba (LC50 was 226.67 μg·mL-1) and Bmp1 (LC50 was 610 μg·mL-1) used alone[155]
Cry1AcB. thuringiensis chitinase(+)对甜菜夜蛾(LC50为4.4 μg·mL-1)和棉铃虫(LC50为5.8 μg·mL-1)的致死毒力较Cry1Ac(LC50分别为6.8和8.8 μg·mL-1)单独使用分别提高1.55和1.52倍;chitinase单独使用的致死率分别为11.7%和8.2% Its lethal virulence to S. exigua (LC50 was 4.4 μg·mL-1) and H. armigera (LC50 was 5.8 μg·mL-1) was 1.55- and 1.52-fold higher than that of the Cry1Ac (LC50 was 6.8 and 8.8 μg·mL-1) used alone, and the lethality rate of chitinase used alone was 11.7% and 8.2%, respectively[22]
Cry1AcNicotiana tabacum chitinase(+)共表达制剂对棉铃虫(LC50为4.79 μg·mL-1)的致死毒力较Cry1Ac(LC50为89.86 μg·mL-1)制剂单独使用提高18.76倍;chitinase单独使用为较低活性Its lethal virulence of coexpression products to H. armigera (LC50 was 4.79 μg·mL-1) was 18.76-fold higher than that of the Cry1Ac (LC50 was 89.86 μg·mL-1) used alone, and the chitinase used alone was low activity[156]
Cry21AaPseudomonas aeruginosa chitinase (pachi)(+)对秀丽隐杆线虫(LC50为30.9 μg·mL-1)的致死毒力较Cry21Aa(LC50为78.7 μg·mL-1)和pachi(LC50为387.3 μg·mL-1)单独使用分别提高2.55和12.53倍Its lethal virulence to C. elegans (LC50 was 30.9 μg·mL-1) was 2.55- and 12.53-fold higher than that of the Cry21Aa (LC50 was 78.7 μg·mL-1) and pachi (LC50 was 387.3 μg·mL-1) used alone[157]
Cry1AcCowpea trypsin inhibitor (CpTI)(+)共表达转基因棉对非靶标害虫棉蚜的防治能力显著增强(原文未显示数值)The ability of coexpression transgenic cotton to control non-target pest Aphis gossypii was significantly enhanced[158]
Cry4BaEnterolobium contortisiliquum trypsin inhibitor (EcTI) (+)对埃及伊蚊的致死率(~85%)较Cry4Ba(~25%)和EcTI(极低活性)单独使用显著提高Its lethality rate to A. aegypti (~85%) was significantly improved than that of the Cry4Ba (~25%) and EcTI (very low activity) used alone[23]
Cry3A-SN-
19 hybrid
Oryza proteinase inhibitor cystatin II (OCII)(+)共表达转基因马铃薯植株对马铃薯甲虫的防控效果显著提高(原文未显示数值)The control effect of coexpression transgenic potato to L. decemlineata was significantly improved (detailed data not shown in article)[159]
Vip3AaA. sativum lectin (ASAL)(+)共表达转基因棉对棉铃虫和烟粉虱的防控效果显著提高(原文未显示数值)The control effect of coexpression transgenic cotton to H. armigera and Bemisia tabaci was significantly improved (detailed data not shown in article)[160]
Cry1AbA. sativum lectin (ASAL)(+)共表达转基因水稻对三化螟、二化螟和稻纵卷叶螟的防控效果显著,虫体死亡率提升87%—93% The control effect of coexpression transgenic rice to S. incertulas, C. suppressalis and C. medinalis was significantly improved, the mortality rate of pests was increased by 87%-93%[24]
Cry1AcGalanthus nivalis lectin (GNA)(+)共表达转基因棉对棉贪夜蛾和棉蚜的致死率提高46.6%—66.2% The lethality rate of coexpression transgenic cotton to S. littoralis and A. gossypii was increased by 46.6%-66.2%[161]
Cry1AcChloroplast transit peptide (TP)(-)+ Ricin B-chain (RB)(+)共表达转基因棉中融合蛋白表达量提高近6倍,且对棉铃虫和棉红铃虫的致死率均提高超过30% The fusion protein expression in coexpression transgenic cotton was improved by nearly 6-fold, and the mortality rate of H. armigera and Pictinophora scutigera was increased by more than 30%[162]
Cry1AcSelenocosmia huwena venom neurotoxin (hwtx-I)(+)共表达制剂对小菜蛾(LC50为5.12 μg·mL-1)的致死毒力较Cry1Ac(LC50为70.78 μg·mL-1)单独使用提高13.8倍;hwtx-I单独使用活性较低The lethal virulence of coexpression products to P. xylostella (LC50 was 5.12 μg·mL-1) was 13.8-fold higher than that of Cry1Ac (LC50 was 70.78 μg·mL-1) used alone, and hwtx-I was low activity when used alone[163]
Cry1AcAustralian funnel-web spider venom neurotoxin (ω-ACTX
-Hv1a)(+)
对甜菜夜蛾(LC50为83.19 μg·mL-1)的致死毒力较Cry1Ac(LC50>500 μg·mL-1)和ω-ACTX-Hv1a(较低活性)单独使用显著增强The lethal virulence to S. exigua (LC50 was 83.19 μg·mL-1) was significantly enhanced than that of Cry1Ac (LC50>500 μg·mL-1) and ω-ACTX-Hv1a (lower activity) used alone[164]
Cry1AcHadronyche versuta venom neurotoxin (hv1a+hv2a)(+)对棉铃虫的致死毒力较Cry1Ac、hv1a和hv2a单独使用分别提高1.32、1.26和1.14倍The lethal virulence to H. armigera was 1.32-, 1.26- and 1.14-fold higher than that of Cry1Ac, hv1a and hv2a used alone[165]
Cry1AcAnemonia viridis neurotoxin (Av3)(+)对棉铃虫(LC50为11.9 μg·mL-1)的致死毒力较Cry1Ac(LC50为30.7 μg·mL-1)和Av3 Cry1Ac(LC50>104 μg·mL-1)单独使用提高2.6倍和超过840倍The lethal virulence to H. armigera (LC50为11.9 μg·mL-1) was 2.6-fold and above 840-fold higher than that of Cry1Ac (LC50 was 30.7 μg·mL-1) and Av3 Cry1Ac (LC50>104 μg·mL-1) used alone[166]
Cry2AbAvermectin(+)对小菜蛾(IC50为0.01 μg·cm-2)的致死毒力较Cry2Ab(IC50为1.544 μg·cm-2)和阿维菌素(IC50为~1 μg·cm-2)单独使用提高154.4和~100倍The lethal virulence to P. xylostella (IC50 was 0.01 μg·cm-2) was 154.4- and ~100-fold higher than that of Cry2Ab (IC50 was 1.544 μg·cm-2) and avermectin (IC50 was ~1 μg·cm-2) used alone[137]
Cry4BaCalcofluor(+)对埃及伊蚊(LC50为0.29 μg·mL-1)的致死毒力较Cry4Ba(LC50为6.27 μg·mL-1)和Calcofluor(致死率<10%)单独使用显著增强The lethal virulence to A. aegypti (LC50 was 0.29 μg·mL-1) was significantly enhanced than that of Cry4Ba (LC50 was 6.27 μg·mL-1) and Calcofluor (lethality rate<10%) used alone[167]
Cyt2BaWhite pepper powder (WP)(+)对白纹伊蚊24 h致死率(80%)较Cyt2Ba(40%)单独使用提高2倍,white pepper powder单独使用活性较低Its lethality rate to A. albopictus (80%) in 24 h was 2-fold higher than that of Cyt2Ba (40%) used alone, and white pepper powder was lower activity when used alone[168]
Cry1-typeMustard oil(+)对欧洲松毛虫致死率(83.4%)较Bt Cry(30%)和mustard oil(6.7%)单独使用分别提高2.78和12.45倍Its lethality rate to Dendrolimus pini (83.4%) was 2.78- and 12.45-fold higher than that of Bt Cry (30%) and mustard oil (6.7%) used alone[138]
Cry1-typeCarvacrol(+)对甜菜夜蛾的致死率(96.7%)较Cry1-type(56.7%)和carvacrol(23.4%)单独使用提高1.71和4.13倍Its lethality rate to S. exigua (96.7%) was 1.71- and 4.13-fold higher than that of Cry1-type (56.7%) and carvacrol (23.4%) used alone[169]
Cry1AcFlavone(+)对棉铃虫的致死率(>90%)较Cry1Ac(<22%,3 ng·cm-2)和flavone(<40%,0.7 mg·g-1)单独使用提高超过68%和50% Its lethality rate to H. armigera (>90%) was above 68% and 50% than that of Cry1Ac (<22%, 3 ng·cm-2) and flavone (<40%, 0.7 mg·g-1) used alone[139]
Cyt2BaB. bassiana(+)共表达制剂对埃及伊蚊和白纹伊蚊的致死毒力较Cyt2Ba单独使用分别提高47%和33%,球孢白僵菌单独使用活性较低The lethal virulence of coexpression products to A. aegypti and A. albopictus was improved by 47% and 33% than that of Cyt2Ba used alone, and B. bassiana was lower activity when used alone[140]
Vip3AB. bassiana (BbV28)(+)共表达制剂对斜纹夜蛾的3 d致死毒力较球孢白僵菌单独使用提高26.2倍,原文Vip3A的活性数据未显示The lethal virulence of coexpression products to S. litura in 3 days was 26.2-fold higher than that of B. bassiana used alone, and the detailed data for Vip3A was not shown in article[170]
Cry3BbPlagiodera versicolora gut bacterium (Pseudomonas putida)(+)对柳蓝叶甲的致死率显著提高(原文未显示数值)Its lethality rate to P. versicolora was significantly improved (detailed data not shown in article)[171]
Cry4BaX. nematophila(+)对埃及伊蚊(LC50为2.02 ng·mL-1)的致死毒力较Cry4Ba(LC50为21.28 ng·mL-1)单独使用提高10.5倍,对埃及伊蚊的致死率(95%)较嗜线虫致病杆菌(52%)单独使用提高43% Its lethal virulence to A. aegypti (LC50 was 2.02 ng·mL-1) was 10.5-fold higher than that of Cry4Ba (LC50 was 21.28 ng·mL-1) used alone, and the lethality rate to A. aegypti (95%) was 43% higher than that of X. nematophila (52%) used alone[141]
Cry4BaP. luminescens(+)对埃及伊蚊(LC50为8.49 ng·mL-1)的致死毒力较Cry4Ba(LC50为21.28 ng·mL-1)提高2.5倍,对埃及伊蚊的致死率(87%)较P. luminescens(43%)单独使用提高44% Its lethal virulence to A. aegypti (LC50 was 8.49 ng·mL-1) was 2.5-fold higher than that of Cry4Ba (LC50 was 21.28 ng·mL-1) used alone, and the lethality rate to A. aegypti (87%) was 44% higher than that of P. luminescens (43%) used alone[141]
Cry4AaB. mori cypovirus (BmCPV) (+)对紫外线的耐受稳定性显著增强,且对白纹伊蚊的致死率显著提高Its tolerance stability to UV radiation was significantly enhanced, and the lethality rate to A. albopictus was significantly increased[172]
Cry1AbAutographa californica multiple nuclearpolyhedrosis virus (AcMNPV)(+)共表达制剂对棉贪夜蛾(LC50为1.7 μg·mL-1)的致死毒力较AcMNPV(LC50为10 μg·mL-1)单独使用提高5.88倍,原文Cry1Ab的活性数据未显示The lethal virulence of coexpression products to S. littoralis (LC50 was 1.7 μg·mL-1) was 5.88-fold higher than that of AcMNPV (LC50 was 10 μg·mL-1) used alone, and the detailed data for Cry1Ab was not shown in article[142]
Cry1-5Araneus ventricosus spider toxin (Av-Tox2)(+)+ AcMNPV(+)共表达制剂对小菜蛾和甜菜夜蛾的致死率提升超过50%和75% The lethality rate of coexpression products to P. xylostella and S. exigua was increased by more than 50% and 75%[25]
Cry1-5Androctonus australis neurotoxin (AaIT)(+)+ AcMNPV(+)共表达制剂对小菜蛾和甜菜夜蛾的致死毒力提升超过9和1.5倍The lethal virulence of coexpression products to P. xylostella and S. exigua was increased by more than 9- and 1.5-fold[143]
Cry1-5Bombus ignites venom Kunitz-type toxin (KTI)(+) +AcMNPV(+)共表达制剂对小菜蛾和甜菜夜蛾的致死率提升超过75%和80% The lethality rate of coexpression products to P. xylostella and S. exigua was increased by more than 75% and 80%[173]
Cry1AbO. furnacalis chymotrypsins- like genes dsRNA (dsCTPs)(+)对亚洲玉米螟的致死率(100%)较Cry1Ab(<60%)和dsCTPs(<20%)单独使用提高超过40%和80% Its lethality rate to O. furnacalis (100%) was increased by above 40% and 80% than that of Cry1Ab (<60%) and dsCTPs (<20%) used alone[144]
Cry1CaS. exigua dsRNA specific to integrin β1 subunit (dsINT)(+)对甜菜夜蛾的致死率(80%)较Cry1Ca(58%)和dsINT(<50%)单独使用提高22%和超过30% Its lethality rate to S. exigua (80%) was increased by 22% and above 30% than that of Cry1Ca (58%) and dsINT (<50%) used alone[174]
表4 基于Bt毒素的抗虫增效应用创新策略
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