| 供试模板 Tested template | 改造位点 Modification site | 实施效果 Implementation effect | 参考文献 Reference |
|---|---|---|---|
| Cry1Aa | Domain I α4—α5区域153位点氨基酸Y突变为C（Y153C）The amino acid Y at site 153 in Domain I α4-α5 was mutated to C (Y153C) | 突变体对烟草天蛾的肠道损伤较原Cry1Aa显著提升The intestinal damage of the mutant to M. sexta was significantly higher than that of the original Cry1Aa | [ |
| Domain II-Loop 2区域氨基酸“RRIILGSGPNNQE”整体突变为人源抗体重链CDR-H3肽段 “GARGDPDFDHSTSYYLDYC” The amino acids “RRIILGSGPNNQE” in Domain II-Loop 2 were mutated to “GARGDPDFDHSTSYYLDYC”, a heavy chain CDR-H3 peptide of human antibody | 突变体对埃及伊蚊（LC50为9.1 μg·mL-1）的致死毒力较原Cry1Aa（LC50>20 μg·mL-1）提高超过2.2倍；对非靶标家蚕的致死率较原Cry1Aa显著降低The lethal virulence of the mutant against A. aegypti (LC50 was 9.1 μg·mL-1) was more than 2.2-fold higher than that of the original Cry1Aa (LC50>20 μg·mL-1). The lethality rate of the mutant to non-target B. mori was significantly lower than that of original Cry1Aa | [ | |
| Domain II-Loop 2区域371LGSG371氨基酸突变371WGLA374 The amino acids 371LGSG371 in Domain II-Loop 2 were mutated to 371WGLA374 | 突变体对家蚕（LC50为0.752 μg·g-1）的致死毒力较原Cry1Aa （LC50为0.864 μg·g-1）提高15% The lethal virulence of the mutant to B. mori (LC50 was 0.752 μg·g-1) was 15% higher than that of the original Cry1Aa (LC50 was 0.864 μg·g-1) | [ | |
| Domain II-Loop 3区域440AA441氨基酸突变为440PR441 The amino acids 440AA441 in Domain II-Loop 3 were mutated to 440PR441 | 突变体对家蚕（LC50为0.398 μg·g-1）的致死毒力较原Cry1Aa（LC50为0.996 μg·g-1）提高2.5倍The lethal virulence of the mutant to B. mori (LC50 was 0.398 μg·g-1) was 2.5-fold higher than that of the original Cry1Aa (LC50 was 0.996 μg·g-1) | [ | |
| Cry1Ab | Domain II Loop2区域282位点氨基酸A、283位点氨基酸L和372位点氨基酸N分别突变为G（A282G）、S（L283S）和A（N372A）The amino acids A at 282 site, L at 283 site and N at 372 site in Domain II Loop2 were mutated to G (A282G), S (L283S) and A (N372A), respectively | 突变体对苹果毒蛾（LC50为8 ng·cm-2）的致死毒力较原Cry1Ab（LC50为290 μg·cm-2）提高36.25倍The lethal virulence of the mutant to Lymantria dispar (LC50 was 8 ng·cm-2) was 36.25-fold higher than that of the original Cry1Ab (LC50 was 290 μg·cm-2) | [ |
| Domain II Loop2 335RRPFNIGINNQ345突变为褐飞虱肠道结合肽GBPs-P2S（CLMSSQAAC）The amino acids 335RRPFNIGINNQ345 in Domain II Loop2 were mutated to Nilaparvata lugens gut-binding peptide GBPs-P2S (CLMSSQAAC) | 突变体对褐飞虱（LC50为21.54 μg·mL-1）的致死毒力较原Cry1Ab（LC50为189.83 μg·mL-1）提高9倍The lethal virulence of the mutant to N. lugens (LC50 was 21.54 μg·mL-1) was 9-fold higher than that of the original Cry1Ab (LC50 was 189.83 μg·mL-1) | [ | |
| Domain III β16区域514位点氨基酸N突变为A（N514A）The amino acid N at 514 site in Domain III β16 was mutated to A (N514A) | 突变体对草地贪夜蛾（LC50为26.4 μg·cm-2）的致死毒力较原Cry1Ab（LC50为468 μg·cm-2）提高18倍The lethal virulence of the mutant to S. frugiperda (LC50 was 26.4 μg·cm-2) was 18-fold higher than that of the original Cry1Ab (LC50 was 468 μg·cm-2) | [ | |
| Domain III β-22区域587位点氨基酸S突变为A（S587A）The amino acid S at 587 site in Domain III β-22 was mutated to A (S587A) | 突变体对草地贪夜蛾（LC50为44 ng·cm-2）的致死毒力较原Cry1Ab（LC50>5000 ng·cm-2）显著增强The lethal virulence of the mutant to S. frugiperda (LC50 was 44 ng·cm-2) was significantly higher than that of the original Cry1Ab (LC50>5000 ng·cm-2) | [ | |
| Cry1Ac | Domain III β16—β17连接区域524位点氨基酸T突变为N（T524N）The amino acid T at 524 site in Domain III β16-β17 connection area was mutated to N (T524N) | 突变体对甜菜夜蛾（LC50为9.6 μg·mL-1）的致死毒力较原Cry1Ac（LC50为14.1 μg·mL-1）提升1.4倍The lethal virulence of the mutant to S. exigua (LC50 was 9.6 μg·mL-1) was 1.4-fold higher than that of the original Cry1Ac (LC50 was 14.1 μg·mL-1) | [ |
| Domain III β18—β19连接区域544位点氨基酸W突变为F（W544F）The amino acid W at 544 site in Domain III β18-β19 connection area was mutated to F (W544F) | 突变体抗虫活性保持不变，但对紫外线的稳定性显著增强The insecticidal activity of the mutant remained unchanged, but its stability to UV radiation was significantly enhanced | [ | |
| Domain III β18—β19连接区域546位点氨基酸N突变为A（N546A）The amino acid N at 546 site in Domain III β18-β19 connection area was mutated to A (N546A) | 突变体对棉铃虫（LC50为1.67 μg·mL-1）的致死毒力较原Cry1Ac（LC50为2.98 μg·mL-1）提高1.78倍The lethal virulence of the mutant to H. armigera (LC50 was 1.67 μg·mL-1) was 1.78-fold higher than that of the original Cry1Ac (LC50 was 2.98 μg·mL-1) | [ | |
| Domain III β20—β21连接区域585位点氨基酸I突变为A（I585A）The amino acid I at 585 site in Domain III β20-β21 connection area was mutated to A (I585A) | 突变体对棉铃虫（LC50为1.47 μg·mL-1）的致死毒力较原Cry1Ac（LC50为2.78 μg·mL-1）提高1.89倍The lethal virulence of the mutant to H. armigera (LC50 was 1.47 μg·mL-1) was 1.89-fold higher than that of the original Cry1Ac (LC50为2.78 μg·mL-1) | [ | |
| Cry1Ai | Domain II Loop2（RIILGSGPNNQE）、Loop3 （TMLSQAAGAVYTL）分别突变为Cry1Ah的Loop2（RPFNIGINNQQ）和Loop3（SMFRSGSSSSVSII）The amino acids of Domain II Loop2 (RIILGSGPNNQE) and Loop3 (TMLSQAAGAVYTL) were mutated to Cry1Ah Loop2 (RPFNIGINNQQ) and Loop3 (SMFRSGSSSSVSII), respectively | 突变体对棉铃虫（LC50为8.6 μg·mL-1）的致死毒力较原Cry1Ai（LC50>500 μg·mL-1）显著提升The lethal virulence of the mutant to H. armigera (LC50 was 8.6 μg·mL-1) was significantly higher than that of the original Cry1Ai (LC50>500 μg·mL-1) | [ |
| Cry1Ca | Domain III区域541STGV544突变为541AAAA544 The amino acids 541STGV544 in Domain III were mutated to 541AAAA544 | 突变体对烟草天蛾（LC50为80 ng·cm-2）的致死毒力较原Cry1Ca（LC50为123 ng·cm-2）提高43% The lethal virulence of the mutant to M. sexta (LC50 was 80 ng·cm-2) was 43% higher than that of the original Cry1Ca (LC50 was 123 ng·cm-2) | [ |
| Cry1Fa | Domain III β16区域507位点氨基酸N突变为A（N507A）The amino acid N at 507 site in Domain III β16 was mutated to A (N507A) | 突变体对草地贪夜蛾（LC50为28.7 ng·cm-2）的致死毒力较原Cry1Fa（LC50为171 ng·cm-2）提高5.95倍The lethal virulence of the mutant to S. frugiperda (LC50 was 28.7 ng·cm-2) was 5.95-fold higher than that of the original Cry1Fa (LC50 was 171 ng·cm-2) | [ |
| Cry2Aa | Domain I α2—α3区域63、64位点氨基酸K分别突变为F（K63F）和P（K64P）The amino acids K at 63 and 64 sites in Domain I α2-α3 were mutated to F (K63F) and P (K64P), respectively | 突变体对斜纹夜蛾、小地老虎和棉铃虫（LC50分别为6.09、72.37和51.33 ng·mL-1）的致死毒力较原Cry2Aa（LC50分别为40.09、299.88和251.33 ng·mL-1）提高4.1—6.6倍The lethal virulence of the mutant to Spodoptera litura, Agrotis ypsilon and H. armigera (LC50 was 6.09, 72.37 and 51.33 ng·mL-1, respectively) was 4.1-6.6 folds higher than that of the original Cry2Aa (LC50 was 40.09, 299.88 and 251.33 ng·mL-1, respectively) | [ |
| Cry2Ab | Domain I α3—α4区域144位点氨基酸L突变为A（L144A）The amino acid L at 144 site in Domain I α3-α4 was mutated to A (L144A) | 突变体对小菜蛾（LD50为1.579 μg·cm-2）的致死毒力较原Cry2Ab（LD50为1.801 μg·cm-2）提高1.14倍The lethal virulence of the mutant to P. xylostella (LD50 was 1.579 μg·cm-2) was 1.14- fold higher than that of the original Cry2Ab (LD50 was 1.801 μg·cm-2) | [ |
| Cry2Ah | Domain II β4—β5之间Loop2区域354位点氨基酸V突变为SP（V354SP）The amino acid V at Loop2 region 354 site in Domain II β4-β5 was mutated to SP (V354SP) | 突变体对棉铃虫（EC50为1.63 μg·mL-1）的致死毒力较原Cry2Ah（EC50为8.7 μg·mL-1）提高5.3倍The lethal virulence of the mutant to H. armigera (EC50 was 1.63 μg·mL-1) was 5.3-fold higher than that of the original Cry2Ah (EC50 was 8.7 μg·mL-1) | [ |
| Cry3Aa | Domain I α3—α4之间的连接区域153NPVSSRNPHS162突变为153NPAPPF-PHS162 The amino acids 153NPVSSRNPHS162 in Domain I α3-α4 connection area were mutated to 153NPAPPF-PHS162 | 突变体对玉米根虫的致死率（93%）较原Cry3Aa（34%）提高2.74倍The lethality rate of the mutant (93%) to D. virgifera was 2.74-fold higher than that of the original Cry3Aa (34%) | [ |
| Domain I区域65、70、231位点氨基酸K以及Domain II区域468位点氨基酸K和Domain III区域596位点氨基酸K均突变为A The amino acids K at 65, 70 and 231 sites in Domain I, 468 site in Domain II and 596 site in Domain III were mutated to A | 突变体对松褐天牛（LC50为12.3 μg·mL-1）的致死毒力较原Cry3Aa（LC50为116.8 μg·mL-1）提高9.5倍The lethal virulence of the mutant to Monochamus alternatus (LC50 was 12.3 μg·mL-1) was 9.5-fold higher than that of the original Cry3Aa (LC50 was 116.8 μg·mL-1) | [ | |
| Domain II Loop1区域345位点氨基酸R、350和351位点氨基酸Y分别突变为A（R345A）、F（Y350F）和F（Y351F）The amino acids R at 345 site, Y at 350 and 351 sites in Domain II Loop1 were mutated to A (R345A), F (Y350F) and F (Y351F), respectively | 突变体对黄粉虫（LD50为1.0 μg）的致死毒力较原Cry3Aa（LD50为11.4 μg）提高11.4倍The lethal virulence of the mutant to Tenebrio molitor (LD50 was 1.0 μg) was 11.4-fold higher than that of the original Cry3Aa (LD50 was 11.4 μg) | [ | |
| Cry4Aa | Domain I α2—α3区域67SG68突变为67SFRRG71、234NR235突变为234NFRR237 The amino acids 67SG68 and 234NR235 in Domain I α2-α3 were mutated to 67SFRRG71 and 234NFRR237, respectively | 突变体对豌豆蚜的致死率（51.1%）较原Cry4Aa（17.8%）提高2.87倍The lethality rate of the mutant (51.1%) to A. pisum was 2.87-fold higher than that of the original Cry4Aa (17.8%) | [ |
| Cry8Ca | Domain II区域439位点氨基酸Q突变为P（Q439P）、Domain III区域884位点氨基酸E突变为G（E884G）The amino acids Q at 439 site in Domain II and E at 884 site in Domain III were mutated to P (Q439P) and G (E884G), respectively | 突变体对铜绿鳃金龟（LC50为0.22334×108 CFU/g）的致死毒力较原Cry8Ca（LC50为0.9583×108 CFU/g）提高4.3倍The lethal virulence of the mutant to Anomala corpulenta (LC50 was 0.22334×108 CFU/g) was 4.3-fold higher than that of the original Cry8Ca (LC50 was 0.9583×108 CFU/g) | [ |
| Cry8Ka | Domain I α3区域82位点氨基酸R突变为Q（R82Q）；Domain II Loop区域260位点氨基酸Y突变为C（Y260C）、321位点氨基酸P突变为A（P321A）；Domain III区域508位点氨基酸R突变为G（R508G）、538位点氨基酸K突变为E（K538E）、594位点氨基酸E突变为N（E594N）The amino acids R at 82 site in Domain I α3, Y at 260 and P at 321 sites in Domain II Loop, and R at 508, K at 538 and E at 594 sites in Domain III were mutated to Q (R82Q), C (Y260C), A (P321A), G (R508G), E (K538E) and N (E594N), respectively | 突变体对棉铃象甲（LC50为2.83 μg·mL-1）的致死毒力较原Cry8Ka（LC50为8.93 μg·mL-1）提高3.15倍The lethal virulence of the mutant to Anthonomus grandis (LC50为2.83 μg·mL-1) was 3.15-fold higher than that of the original Cry8Ka (LC50为8.93 μg·mL-1) | [ |
| Cry19Aa | Domain II Loop 1 355SYWT358突变为355YQDLR359，同时切掉Loop 2的414YPWGD418 The amino acids 355SYWT358 in Domain II Loop 1 were mutated to 355YQDLR359, and at the same time the amino acids 414YPWGD418 in Loop 2 were deleted | 突变体对埃及伊蚊（LC50为3.3 ng·mL-1）的致死毒力较原Cry19Aa（LC50为1.4×105 ng·mL-1）提高42000倍The lethal virulence of the mutant to A. aegypti (LC50 was 3.3 ng·mL-1) was 42000-fold higher than that of the original Cry19Aa (LC50 was 1.4×105 ng·mL-1) | [ |
| Cry41-related | 48位点氨基酸G、59位点氨基酸I、364位点氨基酸K、367和377位点氨基酸Q、378位点氨基酸Y、400位点氨基酸S分别突变为Y（G48Y）、A（I59A）、R（K364R）、K（Q367K）、K（Q377K）、K（Y378K）、Y（S400Y）The amino acids G at 48, I at 59, K at 364, Q at 367 and 377, Y at 378 and S at 400 sites were mutated to Y (G48Y), A (I59A), R (K364R), K (Q367K), K (Q377K), K (Y378K) and Y (S400Y), respectively | 突变体对桃蚜（LC50为19.144 μg·mL-1）的致死毒力较原Cry41-type（LC50为32.7 μg·mL-1）提高1.71倍The lethal virulence of the mutant to Myzus persicae (LC50 was 19.144 μg·mL-1) was 1.71-fold higher than that of the original Cry41-type (LC50 was 32.7 μg·mL-1) | [ |
| Cry51Aa | 46位点氨基酸F、54位点氨基酸Y、95位点氨基酸S、147位点氨基酸F、167位点氨基酸S、219位点氨基酸P、239位点氨基酸N、251位点氨基酸V分别突变为S（F46S）、H（Y54H）、A（S95A）、A（F147A）、R（S167R）、R（P219R）、A（N239A）、A（V251A） The amino acids F at 46, Y at 54, S at 95, F at 147, S at 167, P at 219, N at 239 and V at 251 sites were mutated to S (F46S), H (Y54H), A (S95A), A (F147A), R (S167R), R (P219R), A (N239A) and A (V251A), respectively | 突变体对豆荚草盲蝽（LC50为0.3 μg·mL-1）和美洲牧草盲蝽（LC50为0.85 μg·mL-1）的致死毒力较原Cry51Aa（LC50分别为73和>223 μg·mL-1）提高243和>262倍The lethal virulence of the mutant to Lygus hesperus (LC50 was 0.3 μg·mL-1) and Lygus lineolaris (LC50 was 0.85 μg·mL-1) was 243 and >262 folds higher than that of the original Cry51Aa (LC50 was 73 and >223 μg·mL-1), respectively | [ |
| Cyt1Aa | Loop6-αE区域204位点氨基酸E突变为A（E204A）The amino acid E at 204 site in Loop6-αE was mutated to A (E204A) | 突变体对埃及伊蚊（LC50为103.9 ng·mL-1）的致死毒力较原Cyt1Aa（LC50为952.5 ng·mL-1）提高9.17倍The lethal virulence of the mutant to A. aegypti (LC50 was 103.9 ng·mL-1) was 9.17-fold higher than that of the original Cyt1Aa (LC50 was 952.5 ng·mL-1) | [ |
| Loop6/7/9同时突变为Cry1Ab Domain II Loop3（FRSGFSNSSVSI）The amino acids of Loop6/7/9 were mutated to Cry1Ab Domain II Loop3 (FRSGFSNSSVSI) | 突变体对烟草天蛾和小菜蛾的致死率较原Cyt1Aa提高60%—80% The lethality rate of the mutant to M. sexta and P. xylostella was 60%-80% higher than that of the original Cyt1Aa | [ | |
| Cyt2Aa | Loop4 177SLSAHN181突变为豌豆蚜肠道结合肽GBP3.1（TCSKKYPRSPCM）The amino acids of Loop4 177SLSAHN181 were mutated to A. pisum intestinal binding peptide GBP3.1 (TCSKKYPRSPCM) | 突变体对豌豆蚜和桃蚜（LC50分别为0.18和11.9 μg·mL-1）的致死毒力较原Cyt2Aa（LC50分别为0.37和>150 μg·mL-1）显著增强The lethal virulence of the mutant to A. pisum and M. persicae (LC50 was 0.18 and 11.9 μg·mL-1) was significantly higher than that of the original Cyt2Aa (LC50 was 0.37 and >150 μg·mL-1) | [ |
| Vip3Aa | Domain I和Domain II之间的Loop区域193SS194突变为193RA194、197位点氨基酸K突变为RA（K197RA）The amino acids 193SS194 in Loop area between Domain I and Domain II were mutated to 193RA194, and K at 197 site was mutated to RA (K197RA) | 突变体对草地贪夜蛾和棉铃虫（LC50分别为32.94和148.2 ng·cm-2）的致死毒力较原Vip3Aa（LC50分别为56.76和270.1 ng·cm-2）显著增强The lethal virulence of the mutant to S. frugiperda and H. armigera (LC50 was 32.94 and 148.2 ng·cm-2) was significantly higher than that of the original Vip3Aa (LC50 was 56.76 and 270.1 ng·cm-2) | [ |
| Domain IV区域543位点氨基酸S、544位点氨基酸I和Domain V区域627位点氨基酸E分别突变为N（S543N）、L（I544L）和A（E627A）The amino acids S at 543 and I at 544 sites in Domain IV, and E at 627 site in Domain V were mutated to N (S543N), L (I544L) and A (E627A), respectively | 突变体对草地贪夜蛾（LC50为0.3 μg·g-1）的致死毒力较原Vip3Aa（LC50为2.2 μg·g-1）提高7.3倍The lethal virulence of the mutant to S. frugiperda (LC50 was 0.3 μg·g-1) was 7.3-fold higher than that of the original Vip3Aa (LC50 was 2.2 μg·g-1) | [ | |
| Domain V区域686位点氨基酸S突变为R（S686R）The amino acid S at 686 site in Domain V was mutated to R (S686R) | 突变体对甜菜夜蛾（LC50为2.05 μg·mL-1）的致死毒力较原Vip3Aa（LC50为18.4 μg·mL-1）提高8.98倍The lethal virulence of the mutant to S. exigua (LC50 was 2.05 μg·mL-1) was 8.98-fold higher than that of the original Vip3Aa (LC50 was18.4 μg·mL-1) | [ | |
| Domain V区域776位点氨基酸N突变为Y（N776Y）The amino acid N at 776 site in Domain V was mutated to Y (N776Y) | 突变体对甜菜夜蛾（LC50为110 ng·cm-2）的致死毒力较原Vip3Aa（LC50为250 ng·cm-2）提高2.27倍；且热稳定性显著增强The lethal virulence of the mutant to S. exigua (LC50为was 110 ng·cm-2) was 2.27-fold higher than that of the original Vip3Aa (LC50 was 250 ng·cm-2), and the thermal stability was significantly enhanced | [ | |
| Vip3Af | Domain II区域250位点氨基酸K突变为A（K250A） The amino acid K at 250 site in Domain II was mutated to A (K250A) | 突变体对草地贪夜蛾的致死率（91%）较原Vip3Af（72%）提高19% The lethality rate of the mutant (91%) to S. frugiperda was 19% higher than that of the original Vip3Af (72%) | [ |
| Domain I区域34位点氨基酸M突变为K（M34K）The amino acid M at 34 site in Domain I was mutated to K (M34K) | 突变体对棉贪夜蛾（LC50为12.6 ng·cm-2）的致死毒力较原Vip3Af（LC50为31 ng·cm-2）提高2.46倍The lethal virulence of the mutant to Spodoptera littoralis (LC50 was 12.6 ng·cm-2) was 2.46-fold higher than that of the original Vip3Af (LC50 was 31 ng·cm-2) | [ | |
| Sip1Aa | 153位点氨基酸G和248位点氨基酸H同时突变为C（G153C/H248C）The amino acids G at 153 and H at 248 sites were mutated to C (G153C/H248C) | 突变体对白菜叶甲（LC50为0.614 μg·mL-1）的致死毒力较原Sip1Aa（LC50为1.696 μg·mL-1）提高2.76倍The lethal virulence of the mutant to Colaphellus bowringi (LC50 was 0.614 μg·mL-1) was 2.76-fold higher than that of the original Sip1Aa (LC50 was 1.696 μg·mL-1) | [ |
| β9—β10之间Loop区域128位点氨基酸K突变为A（K128A）The amino acid K at 128 site in the Loop region between β9 and β10 was mutated to A (K128A) | 突变体对白菜叶甲（LC50为0.18 μg·mL-1）的致死毒力较原Sip1Aa（LC50为1.683 μg·mL-1）提高近10倍The lethal virulence of the mutant to C. bowringi (LC50 was 0.18 μg·mL-1) was nearly 10-fold higher than that of the original Sip1Aa (LC50 was 1.683 μg·mL-1) | [ |
