| 供试模板 Tested template | 杂合策略 Heterozygous strategy | 实施效果 Implementation effect | 参考文献 Reference |
|---|---|---|---|
| Cry9Aa: Cry1Ac | Cry9Aa Domain I替换为Cry1Ac的Domain I Domain I of Cry9Aa was replaced by the Domain I of Cry1Ac | 杂合体对棉铃虫（LC50为0.725 ng·cm-2）的致死毒力较Cry1Ac（LC50为3.564 ng·cm-2）提高4.9倍，Cry9Aa无杀虫活性The lethal virulence of the hybrid to H. armigera (LC50 was 0.725 ng·cm-2) was 4.9-fold higher than that of the Cry1Ac (LC50 was 3.564 ng·cm-2), and the Cry9Aa had no insecticidal activity | [ |
| Cry1Ab: Cry1C | Cry1Ab Domain III替换为Cry1C的Domain III Domain III of Cry1Ab was replaced by the Domain III of Cry1C | 杂合体对小菜蛾（LC50为6 ng·cm-2）的致死毒力较Cry1Ab（LC50为15 ng·cm-2）和Cry1C（LC50为117 ng·cm-2）分别提高2.5和19.5倍The lethal virulence of the hybrid to P. xylostella (LC50 was 6 ng·cm-2) was 2.5- and 19.5-fold higher than that of the Cry1Ab (LC50 was 15 ng·cm-2) and Cry1C (LC50 was 117 ng·cm-2), respectively | [ |
| Cry1Ab: Cry1Gc | Cry1Ab Domain III替换为Cry1Gc的Domain III Domain III of Cry1Ab was replaced by the Domain III of Cry1Gc | 杂合体转基因作物对二化螟和亚洲玉米螟的抗性水平显著增强（原文未显示数值）The resistance level of the hybrid transgenic crops to Chilo suppressalis and O. furnacalis was significantly enhanced (detailed data not shown in article) | [ |
| Cry1Ac: Cry1F | Cry1Ac Domain III替换为Cry1F的Domain III Domain III of Cry1Ac was replaced by the Domain III of Cry1F | 杂合体对棉铃虫的致死毒力显著提升（原文未显示数值）The lethal virulence of the hybrid to H. armigera was significantly enhanced (detailed data not shown in article) | [ |
| Cry3Aa: Cry1Ab | Cry3Aa Domain III替换为Cry1Ab的Domain III Domain III of Cry3Aa was replaced by the Domain III of Cry1Ab | 杂合体对玉米根虫的致死毒力显著提升（原文未显示数值）The lethal virulence of the hybrid to D. virgifera was significantly enhanced (detailed data not shown in article) | [ |
| Cry1Jb: Cry1Ab | Cry1Jb Domain III替换为Cry1Ab的Domain III Domain III of Cry1Jb was replaced by the Domain III of Cry1Ab | 杂合体对棉铃虫（LC50为767.62 ng·mL-1）的致死毒力较Cry1Jb（LC50为6039.55 ng·mL-1）提高7.8倍The lethal virulence of the hybrid to H. armigera (LC50 was 767.62 ng·mL-1) was 7.8-fold higher than that of the Cry1Jb (LC50 was 6039.55 ng·mL-1) | [ |
| Cry1Ac: Cry2Ac | Cry1Ac Domain III替换为Cry2Ac7的Domain III Domain III of Cry1Ac was replaced by the Domain III of Cry2Ac7 | 杂合体对大豆夜蛾（LC50为10.20 ng·cm-2）的致死毒力较Cry1Ac（LC50为20.31 ng·cm-2）和Cry2Ac（LC50为46.53 ng·cm-2）分别提高2和4.6倍The lethal virulence of the hybrid to Anticarsia gemmatalis (LC50 was 10.20 ng·cm-2) was 2- and 4.6-fold higher than that of the Cry1Ac (LC50 was 20.31 ng·cm-2) and Cry2Ac (LC50 was 46.53 ng·cm-2), respectively | [ |
| Cry1Ac: Cry1Ca | Cry1Ac Domain III替换为Cry1Ca的Domain III Domain III of Cry1Ac was replaced by the Domain III Cry1Ca | 杂合体对草地贪夜蛾（LD50为288 ng·cm-2）的致死毒力较Cry1Ac（LD50>3000 ng·cm-2）和Cry1Ca（LD50>3000 ng·cm-2）均提高超过10倍The lethal virulence of the hybrid to S. frugiperda (LD50 was 288 ng·cm-2) was above 10-fold higher than that of the Cry1Ac (LD50>3000 ng·cm-2) and Cry1Ca (LD50>3000 ng·cm-2) | [ |
| Cry1Ba: Cry1Ac | Cry1Ba Domain III替换为Cry1Ac的Domain III Domain III of Cry1Ba was replaced by the Domain III of Cry1Ac | 杂合体对棉铃虫（LC50为319 ng·cm-2）的致死毒力较Cry1Ba（LC50>6400 ng·cm-2）提高超过20倍The lethal virulence of the hybrid to H. armigera (LC50为319 ng·cm-2) was above 20-fold higher than that of the Cry1Ba (LC50>6400 ng·cm-2) | [ |
| Cry1Ca: Cry1Ac | Cry1Ca Domain III替换为Cry1Ac的Domain III Domain III of Cry1Ca was replaced by the Domain III of Cry1Ac | 杂合体对棉铃虫（LC50为93 ng·cm-2）的致死毒力较Cry1Ca（LC50>16000 ng·cm-2）提高超过172倍The lethal virulence of the hybrid to H. armigera (LC50 was 93 ng·cm-2) was above 172-fold higher than that of the Cry1Ca (LC50>16000 ng·cm-2) | [ |
| Cry1Ba: Cry1Ca | Cry1Ba Domain III替换为Cry1Ca的Domain III Domain III of Cry1Ba was replaced by the Domain III of Cry1Ca | 杂合体对烟草天蛾（LC50为85 ng·cm-2）的致死毒力较Cry1Ca（LC50为155 ng·cm-2）和Cry1Ba (LC50>1000 ng·cm-2）分别提高1.82倍和超过11倍The lethal virulence of the hybrid to M. sexta (LC50 was 85 ng·cm-2) was 1.82-fold and above 11-fold higher than that of the Cry1Ca (LC50 was 155 ng·cm-2) and Cry1Ba (LC50>1000 ng·cm-2), respectively | [ |
| Cry1Ea: Cry1Ca | Cry1Ea Domain III（530—587）替换为Cry1Ca的Domain III（533—602）Domain III (530-587) of Cry1Ea was replaced by the Domain III (533-602) of Cry1Ca | 杂合体对斜纹夜蛾（EC50为5.8 μg·mL-1）的致死毒力较Cry1Ca（EC50为23.9 μg·mL-1）和Cry1Ea（EC50>108 μg·mL-1）分别提高4.12倍和超过18.62倍The lethal virulence of the hybrid to S. litura (EC50 was 5.8 μg·mL-1) was 4.12-fold and above 18.62-fold higher than that of the Cry1Ca (EC50 was 23.9 μg·mL-1) and Cry1Ea (EC50>108 μg·mL-1), respectively | [ |
| Cry1Gb: Cry1Ig | Cry1Gb Domain III替换为Cry1Ig的Domain III Domain III of Cry1Gb was replaced by the Domain III of Cry1Ig | 杂合体（EC50为54.6 ng·cm-2）对Cry1Fa、Vip3Aa和Cry1A.105/Cry2Ab（EC50>3000 ng·cm-2）耐药性草地贪夜蛾具有显著致死毒力The hybrid showed significant lethal virulence (EC50 was 54.6 ng·cm-2) to S. frugiperda（EC50>3000 ng·cm-2), which was resistant to Cry1Fa, Vip3Aa and Cry1A.105/Cry2Ab | [ |
| Cry1Ia: Cry1Ba | Cry1Ia Domain III替换为Cry1Ba的Domain III Domain III of Cry1Ia was replaced by the Domain III of Cry1Ba | 杂合体对马铃薯甲虫（LC50为22.4 μg·mL-1）的致死毒力较Cry1Ia（LC50为33.7 μg·mL-1）和Cry1Ba（LC50为142 μg·mL-1）分别提高1.5和6.3倍The lethal virulence of the hybrid to L. decemlineata (LC50 was 22.4 μg·mL-1) was 1.5- and 6.3-fold higher than that of the Cry1Ia (LC50 was 33.7 μg·mL-1) and Cry1Ba (LC50 was 142 μg·mL-1), respectively | [ |
| Cry1Aa: Cry1Ab: Cry1Ac | Cry1Aa Domain I拼接Cry1Ab Domain II拼接Cry1Ac Domain III Cry1Aa Domain I spliced with Cry1Ab Domain II and Cry1Ac Domain III | 杂合体对靶标害虫的防控效果显著高于3种母体毒素（原文未显示数值）The control effect of the hybrid to target pests was significantly higher than that of the three original toxins (detailed data not shown in article) | [ |
| Cry1Ac: Cry1Ah: Cry1Ca | Cry1Ac前体肽拼接Cry1Ah Domain I 拼接Cry1Ac Domain II拼接Cry1Ca Domain III Cry1Ac pro-toxin peptide spliced with Cry1Ah Domain I, Cry1Ac Domain II and Cry1Ca Domain III | 杂合体杀虫谱更广，特别是显著增强了对部分豆类作物鳞翅目害虫的致死毒力（数据多未细列）The hybrid had a wider insecticidal spectrum, especially significantly enhanced the lethal virulence to some lepidoptera pests of legume crops (detailed data not shown) | [ |
| Cry1Ab: Cry1Be: Cry1Ka | Cry1Ab前体肽拼接Cry1Be Domain I Domain II and Cry1Ka Domain III | ||
| Cry1Ab: Cry1Ac: Cry1F | Cry1Ab Domain I拼接Cry1Ac Domain II拼接Cry1F Domain III拼接Cry1Ab C端晶体稳定肽（Domain 4—7）Cry1Ab Domain I spliced with Cry1Ac Domain II, Cry1F Domain III and Cry1Ab C-terminal crystal stable peptide (Domain 4-7) | 杂合体对欧洲玉米螟（LC50为0.6 ng·cm-2）的致死毒力较Cry1Ab（LC50为6 ng·cm-2）、Cry1Ac (LC50为7 ng·cm-2）和Cry1Fa（LC50为5 ng·cm-2)分别提高10、11.6和8.3倍The lethal virulence of the hybrid to Ostrinia nubilalis (LC50 was 0.6 ng·cm-2) was 10-, 11.6- and 8.3-fold higher than that of the Cry1Ab (LC50 was 6 ng·cm-2), Cry1Ac (LC50 was 7 ng·cm-2) and Cry1Fa (LC50 was 5 ng·cm-2), respectively | [ |
| Cry1Be: Cry1Ca: Cry1Ab | Cry1Be Domain I—II拼接Cry1Ca Domain III拼接Cry1Ab的C端晶体稳定肽（Domain 4—7）Cry1Be Domain I-II spliced with Cry1Ca Domain III and Cry1Ab C-terminal crystal stable peptide (Domain 4-7) | 杂合体对草地贪夜蛾的致死毒力显著增强，特别对Cry1F/Vip3A抗性品系致死效果更为明显The lethal virulence of the hybrid to S. frugiperda was significantly enhanced, in particular, the lethal effect to Cry1F/Vip3A resistant strain S. frugiperda was more obvious | [ |
| CryAb: Vip3Aa | Cry1Ab的N端（650个氨基酸）与Vip3Aa的C端（790个氨基酸）融合形成复合物The N-terminal of Cry1Ab (650 aa) fused with the C-terminal of Vip3Aa (790 aa) to form a complex | 杂合体转基因水稻对二化螟和稻纵卷叶螟的防治效果显著提升（原文未显示数值）The control effect of the hybrid transgenic rice to C. suppressalis and Cnaphalocrocis medinalis was significantly improved (detailed data not shown in article) | [ |
| Cry4Ba: Cry1Ac | Cry4Ba的N端与Cry1Ac的C端融合形成复合物The N-terminal of Cry4Ba fused with the C-terminal of Cry1Ac to form a complex | 杂合体对尖音库蚊（LC100为2.0 μg·mL-1）的致死毒力较Cry4Ba（LC100>200 μg·mL-1）提高超过100倍。Cry1Ac未见杀虫活性The lethal virulence of the hybrid to C. pipiens (LC100 was 2.0 μg·mL-1) was above 100-fold higher than that of the Cry4Ba (LC100>200 μg·mL-1), and the Cry1Ac had no insecticidal activity | [ |
| Vip3Ca: Vip3Aa | Vip3Ca的N端188个氨基酸等量替换为Vip3Aa的N端氨基酸The 188 N-terminal amino acids of Vip3Ca were replaced by the equivalent N-terminal amino acids of Vip3Aa | 杂合体对草地贪夜蛾（LC50为133.1 ng·cm-2）的致死毒力较Vip3Aa （LC50为162.0 ng·cm-2）和Vip3Ca（LC50>7000 ng·cm-2）分别提高1.22倍和超过52.63倍The lethal virulence of the hybrid to S. frugiperda (LC50 was 133.1 ng·cm-2) was 1.22-fold and above 52.63-fold higher than that of the Vip3Aa (LC50 was 162.0 ng·cm-2) and Vip3Ca (LC50>7000 ng·cm-2), respectively | [ |
| Vip3Aa: Cry1Ac | Vip3Aa16 C端拼接Cry1Ac的N端片段（48----609）The C-terminal of Vip3Aa16 was spliced with the N-terminal fragment (48----609) of Cry1Ac | 杂合体对地中海粉螟的致死率较Vip3Aa提高20%（原文未显示数值）The lethality rate of the hybrid to Ephestia kuehniella was 20% higher than that of Vip3Aa (detailed data not shown in article) | [ |
| Cry2Aa: Cry2Ad | Cry2Aa N端（1—157 bp）与Cry2Ad C端（1743—1899 bp）同源重组杂合体（R27/30）The homologous recombinant of Cry2Aa N-terminal (1-157 bp) and Cry2Ad C-terminal (1743-1899 bp) to form a hybrid (R27/30) | 杂合体（R27/30）对亚洲玉米螟（50 ng·mL-1死亡率为38.33%）的致死毒力较Cry2Aa（50 ng·mL-1死亡率为33.33%）提高近15%。Cry2Ad未见杀虫活性The lethal virulence of the hybrid to O. furnacalis (50 ng·mL-1 with a mortality rate of 38.33%) was 15% higher than that of the Cry2Aa (50 ng·mL-1 with a mortality rate of 33.33%). Cry2Ad had no insecticidal activity | [ |
| Cry2Aa: Photorhabdus luminescens PirB toxin | Cry2A的Domain I替换为PirB toxin基因Domain I of Cry2A was replaced by the gene of PirB toxin | 杂合体对甜菜夜蛾（IC50为0.74 μg·mL-1）的致死毒力较Cry2Aa（IC50为1.78 μg·mL-1）提高2.41倍The lethal virulence of the hybrid to S. exigua (IC50 was 0.74 μg·mL-1) was 2.41-fold higher than that of the Cry2Aa (IC50 was 1.78 μg·mL-1) | [ |
| Cry1Ac: Allium sativum lectin | Cry1Ac Domain III替换为A. sativum lectin基因Domain III of Cry1Ac was replaced by the gene of A. sativum lectin | 杂合体对棉红铃虫（LC100为0.025 μg·g-1）和棉铃虫（LC100为0.5 μg·g-1）的致死毒力分别较Cry1Ac（LC100分别为0.2和15 μg·g-1）提高8和30倍The lethal virulence of the hybrid to P. gossypiella (LC100 was 0.025 μg·g-1) and H. armigera (LC100 was 0.5 μg·g-1) was 8- and 30-fold higher than that of the Cry1Ac (LC100 was 0.2 and 15 μg·g-1), respectively | [ |
| Cry1Ac: Ricin B-chain lectin | Cry1Ac的N端与Ricin B-chain lectin基因融合形成杂合体The N-terminal of Cry1Ac fused with the gene of Ricin B-chain lectin to form a complex | 杂合体对二化螟（致死率90%）的致死毒力较Cry1Ac（致死率30%）提高3倍；杂合体（致死率>90%）对Cry1Ac耐药性棉贪夜蛾的致死毒力较Cry1Ac（致死率<20%）提高超过4.5倍The lethal virulence of the hybrid to C. suppressalis (with a lethality rate of 90%) was 3-fold higher than that of the Cry1Ac (with a lethality rate of 30%), and to Cry1Ac-resistant S. littoralis (with a lethality rate of >90%) was above 4.5-fold higher than that of the Cry1Ac (with a lethality rate of <20%) | [ |
| Cry3Aa: Anoplophora glabripennis midgut Cx-cellulase binding peptide (PCx) | Cry3Aa的N端与PCx基因融合形成杂合体The N-terminal of Cry3Aa fused with the gene of PCx to form a complex | 杂合体对光肩星天牛的致死率（>33%）较Cry3Aa（<13%）提高超过2.5倍。PCx未见杀虫活性The lethality rate of hybrid to A. glabripennis (>33%) was above 2.5-fold higher than that of the Cry3Aa (<13%), and the PCx had no insecticidal activity | [ |
| Cry1B: maize proteinase inhibitor (MPI) potato carboxypeptidase inhibitor (PCI) | MPI拼接Cry1B前体蛋白拼接PCI形成杂合体（MPI-C-PCI） MPI spliced with Cry1B precursor protein and PCI to form a hybrid | 杂合体转基因水稻对二化螟的防控效果显著增强，供试虫体体重较对照减少39.6%—64.6% The control effect of hybrid transgenic rice to C. suppressalis was significantly enhanced, and the body weight of the tested insects was reduced by 39.6%-64.6% compared with that of the control | [ |
