Cotton Science

Figure/Table detail

The control effect and mechanism of Bacillus velezensis EBV02 on cotton Verticillium wilt

Bai Hongyan,Zhao Lihong,Pu Dandan,Feng Zili,Wei Feng,Feng Hongjie,Gu Aixing,Zhu Heqin,Peng Jun,Zhang Yalin

Cotton Science, 2022, 34(5): 443-457. DOI: 10.11963/cs20220019

处理 Treatment	株高 Plant height/cm	单株果枝数 Number of fruit branches per plant	单株结铃数 Number of bolls per plant	30铃籽棉质量 Seed cotton mass of 30 bolls/g	30铃皮棉质量 Lint mass of 30 bolls/g
EBV02浸种 Seed soaking with EBV02	79.87±1.07^*	15.07±0.35	10.53±2.40	163.60±0.56^*	65.50±1.63^*
浸种对照 Seed soaking control	71.20±4.02	14.07±0.29	9.60±0.20	151.00±2.05	60.50±1.47
EBV02灌根 Root irrigation with EBV02	83.07±1.27^*	16.00±1.31	13.87±1.17	169.33±2.05^*	65.40±1.33^*
灌根对照 Root irrigation control	77.87±0.74	15.33±0.35	12.07±1.27	163.80±1.93	61.93±0.90
EBV02喷雾 Spraying with EBV02	73.87±0.84^*	15.80±0.35^*	13.13±0.32^*	175.30±2.17^*	67.80±0.79^*
喷雾对照 Spraying control	70.47±0.07	13.87±0.18	9.13±0.07	163.77±2.88	63.33±1.70

Table 7 Comparisons of cotton yield and yield-related traits between EBV02 treatment and control under field conditions

Other figure/table from this article

Table 1 Specific primer sequences of defense-related genes
Fig. 1 Species identification of the strain EBV02 A, B: colony and cell morphology of the strain EBV02; C: phylogenetic tree of the strain EBV02 based on the sequence of 16S rDNA.
Fig. 2 Inhibitory effect of the strain EBV02 on the hyphae of V. dahliae after 10 d of culture A: blank control of confrontation culture; B: confrontation culture of EBV02; C: blank control of encounter culture; D: encounter culture of EBV02.
Fig. 3 Comparisons of spore production of Verticillium dahliae * indicates significant difference between the treatment and the control (P < 0.05).
Fig. 4 Comparisons of sclerotium germination rates at 20 h of dark culture A, B, C represent cultured with stock solution, 1/2-dilution, 1/4-dilution of EBV02 sterile filtrate, respectively; D: LB medium (control). Different letters indicate significant differences (P < 0.05).
Table 2 Comparisons of germination rates and shoot length between seed soaking treatments
Table 3 Control effect of EBV02 on cotton Verticillium wilt at 15 d after inoculation with V. dahliae in a greenhouse
Fig. 5 Control effect of EBV02 treatment on cotton Verticillium wilt in a greenhouse A: seed soaking control; B: seed soaking with EBV02; C: root irrigation control; D: root irrigation with EBV02.
Table 4 Comparison of cotton growth indexes of different treatments at 25 d after seedling emergence
Table 5 Field control effect of the strain EBV02 on cotton Verticillium wilt at 60 d after sowing
Table 6 Comparisons of cotton emergence rates and growth indexes between seed soaking treatments
Fig. 6 Performance of EBV02 enhanced cotton leaves against V. dahliae A: Root irrigation control; B: root irrigation with EBV02.
Fig. 7 Performance of EBV02 triggered the burst of reactive oxygen species in cotton A: Root irrigation control; B: root irrigation with EBV02.
Fig. 8 EBV02 induced callose accumulation in cotton leaves A: Root irrigation control; B: root irrigation with EBV02.
Fig. 9 The expression levels of defense-related genes A-E: the relative expression levels of POD, PPO, PAL, PR10, JAR1; control meant cotton first treated with LB medium, and then inoculated with Vd080. EBV02 meant cotton first treated with EBV02 culture solution and then inoculated with Vd080. * indicates significant difference between the treatment and the control (P<0.05).

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