Cotton Science

Figure/Table detail

The control effect and mechanism of Bacillus velezensis EBV02 on cotton Verticillium wilt

Bai Hongyan,Zhao Lihong,Pu Dandan,Feng Zili,Wei Feng,Feng Hongjie,Gu Aixing,Zhu Heqin,Peng Jun,Zhang Yalin

Cotton Science, 2022, 34(5): 443-457. DOI: 10.11963/cs20220019

基因名 Gene name	引物序列 Primer sequence
POD	F: CCGCATAACCATCACAAG	R: ACTCTCATCACCTTCAACA
PPO	F: ATATCCTTGTTCTGTCTGCTA	R: CTCCTTCTACCGTCTCTTC
PAL	F: TGGTGGCTGAGTTTAGGAAA	R: TGAGTGAGGCAATGTGTGA
PR10	F: ATGATTGAAGGTCGGCCTTTAGGG	R: CAGCTGCCACAAACTGGTTCTCAT
JAR1	F: ATGAGGATGTTGGAGAAGAT	R: TCAGTTGAAAGCAGTACTAAAGT
ubiquitin	F: GAGTCTTCGGACACCATTG	R: CTTGACCTTCTTCTTCTTGTGC

Table 1 Specific primer sequences of defense-related genes

Other figure/table from this article

Fig. 1 Species identification of the strain EBV02 A, B: colony and cell morphology of the strain EBV02; C: phylogenetic tree of the strain EBV02 based on the sequence of 16S rDNA.
Fig. 2 Inhibitory effect of the strain EBV02 on the hyphae of V. dahliae after 10 d of culture A: blank control of confrontation culture; B: confrontation culture of EBV02; C: blank control of encounter culture; D: encounter culture of EBV02.
Fig. 3 Comparisons of spore production of Verticillium dahliae * indicates significant difference between the treatment and the control (P < 0.05).
Fig. 4 Comparisons of sclerotium germination rates at 20 h of dark culture A, B, C represent cultured with stock solution, 1/2-dilution, 1/4-dilution of EBV02 sterile filtrate, respectively; D: LB medium (control). Different letters indicate significant differences (P < 0.05).
Table 2 Comparisons of germination rates and shoot length between seed soaking treatments
Table 3 Control effect of EBV02 on cotton Verticillium wilt at 15 d after inoculation with V. dahliae in a greenhouse
Fig. 5 Control effect of EBV02 treatment on cotton Verticillium wilt in a greenhouse A: seed soaking control; B: seed soaking with EBV02; C: root irrigation control; D: root irrigation with EBV02.
Table 4 Comparison of cotton growth indexes of different treatments at 25 d after seedling emergence
Table 5 Field control effect of the strain EBV02 on cotton Verticillium wilt at 60 d after sowing
Table 6 Comparisons of cotton emergence rates and growth indexes between seed soaking treatments
Table 7 Comparisons of cotton yield and yield-related traits between EBV02 treatment and control under field conditions
Fig. 6 Performance of EBV02 enhanced cotton leaves against V. dahliae A: Root irrigation control; B: root irrigation with EBV02.
Fig. 7 Performance of EBV02 triggered the burst of reactive oxygen species in cotton A: Root irrigation control; B: root irrigation with EBV02.
Fig. 8 EBV02 induced callose accumulation in cotton leaves A: Root irrigation control; B: root irrigation with EBV02.
Fig. 9 The expression levels of defense-related genes A-E: the relative expression levels of POD, PPO, PAL, PR10, JAR1; control meant cotton first treated with LB medium, and then inoculated with Vd080. EBV02 meant cotton first treated with EBV02 culture solution and then inoculated with Vd080. * indicates significant difference between the treatment and the control (P<0.05).

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