In order to reveal the difference between thick root (TR) and storage root (SR) of sweetpotato with difference drought tolerance from transcriptional level, and further explicit the mechanism of roots differentiation and drought tolerance by simulating field drought in dry shed with artificial water control, two varieties including ‘Jishu21’ (‘JS21’ ) with strong drought tolerance and ‘Jizishu1’ (‘JZ1’) with weak drought tolerance were chosen as research materials. Transcriptome analysis of sweet potato thick root and storage root was carried out. The results indicated GO classification of differentially expressed genes (DEGs) of the two varieties was similar to that of KEGG enrichment. Under drought stress, 84 DEGs between SR and TR of ‘JS21’ mainly enriched the accumulation of starch and sucrose metabolic pathway, while 112 DEGs between SR and TR of ‘JZ1’ mainly enriched ribosomal metabolic pathway. Among different varieties, 224 DEGs between ‘JS21’ and ‘JZ1’ were found in SR, but 204 DEGs between ‘JS21’ and ‘JZ1’ were counted in TR, and all mainly enriched ribosomal metabolic pathway. Further analysis by transcription factors and hormones metabolism showed that ARF-like transcription factors in storage root and NAC-like transcription factors in thick root of ‘JS21’ were more differentially expressed than ‘JZ1’ under drought stress. The expression of genes involved in carotenoid biosynthesis pathway was up-regulated, while genes involved in brassinosteroid biosynthesis pathway were down-regulated in both thick root and storage root of ‘JS21’. In the same variety, the gene expression of α-linolenic acid metabolic pathway in ‘JS21’ storage root was up-regulated compared with thick root. In conclusion, sweetpotato with strong drought tolerance can maintain starch and sucrose metabolism under drought conditions by coordinating the expression of drought tolerance genes and growth-related transcription factors, balancing drought tolerance hormones (ABA, JA) and growth related hormones (IAA, BR), so as to improve the drought resistance and ensure the formation of yield.

In order to explore the difference of selenium enrichment ability of different sweet potato strains, 22 excellent sweet potato strains independently selected by Fuzhou Institute of Agricultural Sciences were used as test materials, and ‘Guangshu 87’ with strong selenium enrichment ability was used as the control variety. 22 independently bred sweet potato strains were selected as materials, and ‘Guangshu 87’ was used as the control variety. The comparative experiment of selenium enrichment ability was carried out by spraying exogenous organic selenium fertilizer on the leaves. The results showed that: (1) Cluster analysis of selenium content in sweet potato tubers of different strains treated with selenium fertilizer showed that the strains with high enrichment and medium high enrichment types included nine strains such as ‘Rongshu 2306’, ‘Rongshu 2321’, ‘Rongshu 2310’, ‘Rongshu 2305’, ‘Rongshu 2322’ and so on. ‘Rongshu 2306’, ‘Rongshu 2301’, ‘Guangshu 87’, ‘Rongshu 2315’ and ‘Rongshu 2321’ ranked the top five in the increase rate of selenium content in tubers compared with the control. (2) There were differences in selenium accumulation of sweet potato tubers among different strains treated with selenium fertilizer. The top five strains included ‘Rongshu 2306’, ‘Guangshu 87’, ‘Rongshu 2315’, ‘Rongshu 2301’ and ‘Rongshu 2310’. The selenium absorption and utilization rates of different strains of sweet potato tubers were quite different. The strain with absorption and utilization rates of more than 5% was ‘Rongshu 2306’, and the strains with absorption and utilization rates of more than 3% included ‘Rongshu 2301’, ‘Rongshu 2315’, ‘Guangshu 87’, ‘Rongshu 2321’, ‘Rongshu 2310’ and so on. (3) Cluster analysis of selenium sensitivity of different sweet potato strains showed that ‘Rongshu 2306’, ‘Rongshu 2315’ and ‘Rongshu 2301’ were more sensitive to selenium. The selenium sensitivity index was between 0.89 and 0.91, and the yield increase rate of selenium fertilizer treatment was between 9.82% and 12.48% compared with the control. In conclusion, ‘Rongshu 2306’, ‘Rongshu 2301’ and ‘Rongshu 2315’ had strong selenium enrichment ability and high fresh tuber yield, which were suitable for planting as selenium-enriched sweet potato varieties and parent materials for breeding new selenium-enriched sweet potato varieties.

This study investigated the effects of different fertilization treatments on sweet potato growth, nutrient utilization, and soil quality using liquid fertilizers compounded from hydrothermal cracking products of agricultural and forestry residues, to provide a scientific basis for the application of hydrothermal cracking liquid fertilizers in sweet potato cultivation. A randomized block design was conducted with five treatments: no fertilizer (CK), conventional solid water-soluble fertilizer (CF), conventional solid water-soluble fertilizer plus micro-element liquid fertilizer (CFMI), macro-element liquid fertilizer (MA), and macro-element plus micro-element liquid fertilizer (MAMI). Quantitative analyses were conducted to evaluate the impact of these fertilization treatments on sweet potato yield and quality, economic benefits, nutrient absorption and utilization efficiency, and soil physicochemical properties. The results showed that compared to the CK treatment, all fertilization treatments significantly enhanced sweet potato yield, quality and economic benefits. Compared to the CF treatment, the application of hydrothermal cracking liquid fertilizers significantly increased the number of marketable roots per plant, individual root weight, fresh root yield, marketable root yield and the proportion of marketable roots, as well as the content of soluble sugar, vitamin C and carotene. Particularly under the MAMI treatment, the fresh root yield and marketable root yield increased by 13.7% and 19.8%, respectively, and the output/input ratio improved by 16.3%, demonstrating significant enhancement of yield and economic benefits. Additionally, the use of hydrothermal cracking liquid fertilizers did not significantly reduce soil pH nor did it lead to excessive nutrient accumulation. However, it enhanced nutrient (N, P, K) utilization efficiency and agronomic efficiency, thereby reducing resource waste. In summary, liquid fertilizers compounded from hydrothermal cracking products of agricultural and forestry residues are highly efficient, which can promote the yield and quality of sweet potatoes and improve the soil environment. The results support the potential agricultural application of hydrothermal cracking liquid fertilizers in sustainable sweet potato cultivation.

Re-domestication of diploid potato (Solanum tuberosum) into a seed crop is an innovative breeding method to accelerate genetic improvement. Seed propagation would allow hybrid production and mix superior alleles. However, almost all diploid potatoes in nature are self-incompatible (SI). Gametophytic self-incompatible (GSI) is a widespread SI in Solanaceae and is controlled by the S locus that contains a ribonuclease (S-RNase) and multiple F-box (SLFs); however, the genetic diversity of the S locus in potato is unclear. In this study, we identified 21 S-RNase alleles involved in SI from 194 diploid potato accessions by large-scale transcriptome sequencing. The levels of amino acid similarity among different S-RNase proteins varied from 31.3 to 95.8%. S2 alleles is the most widespread in 194 diploid potato and is mainly distributed in the S. tuberosum Group Phureja. Based on genomic annotation and expression analysis, we identified 12 potential functional SI male-determinant genes, S-locus F-box (SLFs), encoding F-box proteins in the S2 locus on a genomic region of approximately 13 Mb. Comparative genomics analysis showed that eight SLF genes are relatively conserved among four homozygous S locus. The Ka and Ks analysis suggested that S-RNase and intra-haplotypic SLF genes have undergone co-evolved. These findings can not only help to select suitable pollinators but also to combine more hybrid combinations and make full use of heterosis to accelerate diploid potato breeding.

The TSJT1 protein belongs to the superfamily of class-II glutamine amidotransferases (GATase). There is little research on the functions and underlying mechanisms of TSJT1 in plants.  In this study, an abscisic acid (ABA)-inducible gene IbTSJT1 was isolated from drought-tolerant sweetpotato line ‘Xushu55-2’.  Its expression was strongly induced by PEG6000 and ABA.  The IbTSJT1 protein was localized in the nucleus and cell membrane.  The IbTSJT1-overexpressing sweetpotato plants exhibited significantly enhanced drought tolerance.  Their ABA and proline contents and superoxide dismutase (SOD) and peroxidase (POD) activities were increased and reactive oxygen species (ROS) scavenging-related genes were upregulated under drought stress.  The stomatal aperture assay confirmed that the IbTSJT1-overexpressing plants increased the sensitivity to ABA.  Yeast one-hybrid (Y1H) assay, electrophoretic mobility shift assay (EMSA), luciferase reporter assay and ChIP-qPCR assay indicated that IbABF2 could directly bind to the cis-acting ABA-responsive element (ABRE) in the IbTSJT1 promoter to activate the expression of IbTSJT1.  These findings suggest that IbTSJT1 mediates ABA-dependent drought stress responses and enhances drought tolerance by inducing stomatal closure and activating ROS scavenging system in transgenic sweetpotato.  Our study provides a novel gene for improving drought tolerance of sweetpotato and other plants. 

To improve the grain and oil yield per unit land area, suitable soybean varieties under the potato, corn and soybean compound planting model in shallow mountain and hilly areas were screened. 2 belt types (1.67 and 2.00 m) of potato, corn, and soybean compound planting models were set up, and comprehensive analysis and evaluation were conducted on the growth period, biological traits, agronomic traits, and yield traits of 9 soybean varieties (Qindou 2018, Qihuang 34, Shankendou 4, Zhonghuang 13, Shandou 125, Yandou 15, Yandou 11, Baodou 12, and Baodou 312). The results showed that there was no significant difference in the maturity and growth stages of various soybean varieties under different belt type compound planting models. Belt types had a certain impact on the growth habits and lodging resistance of soybean plants. As the width of the band increased, the lodging levels of Qihuang 34, Zhonghuang 13 (CK), Yandou 15, and Baodou 312 decreased. The main agronomic traits such as plant height and pod height of various soybean varieties did not show significant changes under different intercropping patterns. Each variety was affected by different degrees of diseases and pests, and attention should be paid to preventing and controlling diseases and pests in production. The yield of Qihuang 34 was higher under the 1.67 and 2.00 m belt type compound planting models, with 886.67 and 1 150.57 kg/hm², respectively. Overall, Qihuang 34 has lodging resistance and performs well in comprehensive agronomic traits, promoting planting the potato, corn and soybean compound planting model in the shallow mountain and hilly areas.

The study aims to investigate the effect of potato starch that is irradiated by γ-ray radiation on the properties of starch film. Starch was irradiated by ⁶⁰Co-γ-ray radiation with different absorbed doses (0-50 kGy) and the starch films were made. The prepared films were characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The results showed that γ-ray radiation caused the breakage of glucoside bond and the breakdown of starch long chain molecules. The tensile strength and elongation at break of starch film increased and then decreased with increasing absorbed doses, the light transparent of starch film decreased and then increased. When the absorbed dose was 10 kGy, the mechanical properties of the film was the best, the elongation at break and the tensile strength were 62.37% and 3.73 Mpa respectively, however, the light transmission of the film was the worst, with the opacity value of 1.29. The solubility of film increased with the increase of absorbed dose. Insoluble starch granules decreased and the surface of starch films became more and more smoothed with increasing absorbed does. γ-ray radiation could effectively destroy the molecular structure and break starch molecular chain. The mechanical properties, light transmittance, water solubility and film forming properties of starch film could be significantly changed by irradiation.

To select suitable potato varieties with different maturity periods for cultivation in the Western Sichuan Plateau, this study analyzed 12 yield and quality related traits across 18 potato varieties of different maturity periods, and further comprehensively evaluated their performance of yield and quality by combining multiple linear regression and correlation analysis. Our results showed that in early-maturing varieties, the variation coefficients of the number of tubers per plant, average single tuber weight, and vitamin C content were more than 30%, yield was significantly positively correlated with the number of main stems, and commodity potato rate was significantly positively correlated with average single tuber weight. There was a significant negative correlation between the commodity potato rate and both the number of tubers per plant and vitamin C content. In mid-late-maturing varieties, the variation coefficients of the number of main stems, vitamin C, and reducing sugar content were more than 30%, the commodity potato rate was significantly positively correlated with average single tuber weight, while growth period was significantly negatively correlated with plant height, starch, and reducing sugar content. This study clarified that the yield of early-maturing varieties was related to the number of main stems, number of tubers per plant and commodity potato rate, while the yield of mid--late-maturing varieties waas related to the commodity potato rate and number of tubers per plant. These findings provided a reference for screening and breeding of potato varieties in the Western Sichuan Plateau.

To investigate the effects of different organic fertilizers on the yield and quality of purple sweet potatoes, a field experiment was conducted in 2023 at the experimental base of Tai’an Academy of Agricultural Sciences using ‘Taizishu 1’ as the test sweet potato variety. Six treatments were designed, including no fertilization, single chemical fertilizer, and 50% reduced chemical fertilizer combined with pig manure, cow manure, sheep manure, and chicken manure. The study analyzed the impacts of these treatments on agronomic traits, dry matter distribution, yield, and quality of purple sweet potatoes. The results showed that the purple sweet potato stem treated with pig manure had the thickest diameter, at 14.95 cm, an increase of 15.71% compared to the single chemical fertilizer treatment; the aboveground dry weight of sheep manure treatment was the heaviest, at 197.62 g, 30.33% higher than the single chemical fertilizer treatment; the proportion of dry matter distribution in the tubers treated with cow manure was the highest, at 71.82%, which increased by 11.47% compared to the single chemical fertilizer treatment; the yield and commodity rate of purple sweet potato tubers treated with pig manure were the highest, which were 48607.95 kg/hm² and 79.25%, respectively; the soluble sugar content in purple sweet potato tubers treated with pig manure was the highest, at 38.75 mg/g, significantly higher than other treatments. Research has shown that the combination of pig manure and chemical fertilizers can promote the coordinated growth of the aboveground and underground parts of purple sweet potatoes, promote the rational distribution of dry matter, and thereby increase the quantity, rate, weight, yield, and soluble sugar content of commercial sweet potatoes. The effect is superior to the combination of other organic fertilizers and chemical fertilizers. This research provides a theoretical foundation for high-quality and high-yield cultivation techniques of purple sweet potatoes.

Biogenic pesticides have a good control effects on crop diseases and pests, and can effectively replace some chemical pesticides, which is a new research direction in the pesticide industry. 3 biological pesticides, eugenol, carvacrol, and polyoxin, as well as fipronil manganese zinc chemicals, were selected as the research objects to spray potatoes at different periods, and the control effects of the 4 pesticides on potato early blight, late blight, black scurf and scab disease were compared.The experimental results showed that in terms of early blight control efficiency of the crop, the control efficiency of the 4 pesticides was ranked as carvacrol> fipronil manganese zinc> polyoxin> eugenol; in terms of late blight prevention effectiveness, the order was carvacrol> eugenol> fipronil manganese zinc> polyoxin; in terms of black scurf prevention effectiveness, the order was carvacrol> eugenol> polyoxin> fipronil manganese zinc; in terms of scab disease prevention effectiveness, the order was fipronil manganese zinc> carvacrol> eugenol> polyoxin. In conclusion, comparing the comprehensive control effect of three biological pesticides and fipronil manganese zinc on 4 diseases, carvacrol was found to be the best. This article provides references for exploring the application methods of bio-derived pesticides, reduce the dosage of chemical pesticides, and promoting the green integrated control technology of potato.

In order to provide reference for rational fertilization and green disease prevention and control in flue-cured tobacco, the effects of boron and silicon nutrient elements on the diseases prevention and control and the physiological mechanism were discussed. The field experiment was conducted to study the effects of different boron and silicon application levels on the incidence of potato virus Y (PVY) disease and defense enzyme activity of flue-cured tobacco named ‘Yunyan 100’. The results showed that the application of boron and silicon reduced the incidence and disease index of PVY in flue-cured tobacco. Compared with the treatment of spraying water (CK), the incidence and disease index of PVY under 9.0 kg/hm² boron+ 6.0 kg/hm² silicon treatment decreased by 32.5% and 22%, respectively. Meanwhile, reasonable application of boron and silicon fertilizer significantly improved the defense enzymes activities of tobacco leaves, and the phenylalanine degradation enzyme activity and proline content were significantly increased by 121.4%-426.5% and 30.0%-74.3%, respectively. Correlation analysis showed that the incidence of PVY was significantly negatively correlated with proline content, while phenylalanine degradation enzyme activity was significantly positively correlated with proline content. The combined application of 9.0 kg/hm² boron+ 6.0 kg/hm² silicon could reduce the occurrence of diseases and induce the increase of defense enzyme activity.

【Objective】Seeking key indicators and methods for accurately characterize drought tolerance in sweet potato, and screening and identifying drought-tolerant sweet potato germplasm resources, to provide effective methods for the rapid and accurate identification of drought-tolerant sweet potato germplasm resources, and to provide material and theoretical basis for selection and breeding of high quality and drought-tolerant sweet potato varieties. 【Method】Fifty-four sweet potato germplasm resources were used as materials for drought stress experiments. By using two treatments including drought stress and control, and combining with drought pool cultivation experiment and field test, the effects of drought stress on the growth and development, physiological and biochemical characteristics, antioxidant metabolism, photosynthetic characteristics and yield of different sweet potato germplasm resources were investigated, the response characteristics of different sweet potato germplasm resources to drought were analyzed, and the effective indicators for drought tolerance evaluation in sweet potato were selected. The drought tolerance evaluation was preformed using principal component analysis, correlation analysis, direct evaluation of drought resistance coefficient, and calculation of comprehensive drought tolerance measurement value (D value) based on membership function, and the drought-tolerant sweet potato germplasm resources were screened and identified.【Result】The results obtained from the drought pool cultivation experiment showed the influences of drought treatment on the main stem length, aboveground fresh weight, underground dry weight and fresh weight of storage root were extremely significant (P<0.01), and eight drought-tolerant germplasm resources were screened based on cluster analysis of D values. In the field test, the main stem length, stem diameter, number of branches, leaf area index, leaf relative water content, total chlorophyll content, stomatal conductance, net photosynthetic rate, transpiration rate, intercellular carbon dioxide concentration, proline (Pro), malondialdehyde (MAD), peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) showed highly significant differences (P<0.01) under drought stress when compared with control. Through the establishment of regression models, it could be initially determined that eight indicators including the leaf area index, root tip, leaf POD, leaf APX, storage root Pro, storage root SOD, storage root CAT, and yield could be used as indicators for drought tolerance identification in sweet potato. XN18111-1, 20XN18-1, XN1834-11 and XN17104-46 were classified as drought-tolerant germplasm resources according to grading of drought resistance coefficient based on yield. The D values of XN18111-1, 20XN18-1 and XN1862-61 were over 0.6 and showed high drought tolerance based on comprehensive drought tolerance evaluation. 【Conclusion】Based on results of comprehensive drought tolerance evaluation in drought pool cultivation experiment, as well as the comprehensive drought tolerance evaluation and yield evaluation in field test, XN18111-1 and 20XN18-1 were finally identified as drought-tolerant germplasm resources, which can be used as drought-tolerant breeding materials or ideal resource materials for study on drought-tolerance mechanism in sweet potato.

The purpose of this study was to investigate the phenotypic and main quality changes of nine yellow-fleshed sweetpotato (YFSP) varieties at different development stages, and to provide a theoretical basis for the breeding of fresh sweetpotato. Nine winter YFSP varieties were used as materials. The diameter, length, weight, flesh color, dry rate, starch, and soluble sugar content of sweetpotato at five development stages were measured and analyzed. The results showed that the diameter, length, weight and dry rate of single potato increased continuously during the development process. The growth rate of sweetpotato length of ‘Guangshu 79’ was the fastest at 45-105 d, and that of ‘Anna’ was the fastest at 135-165 d. The highest rate of increase in diameter and single sweetpotato weight was ‘Mashali’. The dry matter rate of ‘Guangshu 79’ increased the fastest. The soluble sugar content showed a downward trend during development, and ‘Guangshu 79’ showed the fastest decline. With the increase of starch content, ‘Guangshu 79’ increased rapidly in the early growth period of 45-105d, and ‘Anna’ increased fastest in 135-165d. The study found that the length, diameter, single root weight, dry rate and total starch content of YFSP tubers increased gradually with the growth and development period, and the content of soluble sugar decreased gradually. The rapid development and growth periods of different varieties of sweet potato were different.

To evaluate the field control effects of several chemical control agents on potato late blight in Matou Town of Tancheng County, Linyi City, Shandong Province, 687.5 g/L fluopyram-penoxifen wettable powder, 72% mancozeb-manganese zinc, 42.8% fluopyram-iprovalent wettable powder, and 10 billion/g Bacillus subtilis biological preparation were selected, and a control group and treatment groups were set up, and the incidence index, control effect, and yield were monitored. The results showed that the 687.5 g/L fluopyram-penoxifen wettable powder had a significant control effect on the disease. In 2020, the incidence index was 3.21, 5.13, and 6.02 after three applications of the treatment, with an average control effect of over 80% and a maximum of 85.14%, and an increase in yield of 27.9%. In 2021, the incidence index was 2.95, 4.75, and 5.45 after three applications of the treatment, with an average control effect of over 84% and an increase in yield of 26.0%. The 42.8% fluopyram-iprovalent wettable powder had a slightly lower control effect but was better than other pesticides. The importance of selecting control agents based on regional specific conditions was emphasized, and fluopyram-penoxifen wettable powder was recommended as the preferred control agent for late blight of potato in the study area. The research results provide scientific control guidelines for farmers.

In order to explore the effects of cultivation density and nitrogen fertilizer application on potato yield and quality in high-altitude areas, a two factor split plot experimental design was adopted. Three density levels (41700, 50000, 62500 plant/hm², represented as D₁, D₂, D₃ respectively) and four nitrogen application levels (0, 110, 220, 330 kg/hm², represented as N₀, N₁, N₂, and N₃ respectively) were set up to analyze different densities and the effect of nitrogen fertilizer treatment combination on the plant traits, yield, and quality of the late maturing potato variety ‘Zangnongshu 1’, and explore the optimal fertilizer density configuration for this variety. The results showed that density and nitrogen fertilizer had a significant impact on the traits, yield, and quality of potato plants, and the yield and quality were significantly affected by the interaction between density and nitrogen fertilizer. The potato yield and commercial potato yield under low density of 41700 plants/hm² increased with the increase of nitrogen application. The dry matter content, starch content, and protein content showed a trend of first increasing and then decreasing with the increase of nitrogen application. The potato yield and commercial potato yield under high density of 50000 plants and high density of 62500 plants/hm² increased and then decreased with the increase of nitrogen application, while the dry matter content, starch content, and protein content showed an upward trend. The highest potato yield under D₂N₂ treatment was 47884 kg/hm², with a commercial potato yield of 56.03%, and good performance in plant and quality traits. Taking into account plant, yield, and quality traits, based on TOPSIS analysis results, it is more suitable to plant potatoes in Lhasa and its surrounding areas with a density of 50000 plants/hm² and a nitrogen application rate of 220 kg/hm².

In order to explore a new intercropping model that suitable for the flatland and shallow hill in Chongqing and similar ecological areas, increase the yield and economic benefits of crops, and provide reference for research on crop intercropping models, an intercropping experiment with fresh maize, sweet potato, and soybean was conducted. In this experiment, three different row ratios of maize-sweet potato-soybean intercropping models were used, with one fresh waxy maize variety, three fresh sweet potato varieties, and one fresh soybean variety as materials. The results showed that there were no significant differences in the yield components of fresh waxy maize in different row ratio intercropping models, but there was a significant difference in the average fresh ear weight per plant and yield. There were no significant differences in the number of branches, effective pod number per plant, and fresh pod yield of fresh soybean in different row ratio intercropping models. In the intercropping system, sweet potatoes in different row ratio intercropping were affected by the shading of maize, resulting in varying degrees of reduction in storage root yield. The different row ratio intercropping models had inconsistent effects on the number of storage root per plant and commodity rate of different sweet potato varieties. Increasing the intercropping planting density of fresh sweet potato and waxy maize properly is beneficial for achieving high yields. Using the row ratio model of 2:2:3, and intercropping fresh waxy maize and fresh soybean with sweet potato variety ‘Pushu32’ can achieve the highest total economic benefits.

The study aims to solve the lack of high starch processing potato varieties with high yield and good quality in Hulunbuir area. In 2022 to 2023, using ‘Weilasi’ as the control, the phenophase and tuber traits of 8 potato varieties were investigated, and the performance and stability of starch content, tuber yield and starch yield were identified and evaluated. Among the 9 cultivars, the starch content of ‘Zhong Shu 49’ and ‘Long Shu 20’ ranked first and second respectively, and the average values of the two years was 18.27 and 18.10 respectively, which were higher than those of the control, but the difference was not significant. ‘Long Shu 20’and ‘Zhong Shu 28’ had high tuber yield of 55074 kg/hm² and 54607 kg/hm² respectively, which were significantly higher than that of the control, and the stability was good. The highest tuber starch yield of ‘Long Shu 20’ was 9863 kg/hm², which was significantly higher than that of the control and other cultivars, and the performance was stable. ‘Long Shu 20’ had the highest starch yield and stable performance among the nine cultivars, which was suitable for demonstration and promotion as a starch processing variety in this region.

The input-output data of potato in S Province from 2011 to 2022 were selected, and the data envelop analysis method was used to explore their production benefits and efficiency. The results showed that the overall cost of potato production in the study area had been increasing year by year, with significant increases in cost elements such as labor, seed, fertilizer, and land transfer; The profit margin fluctuates greatly, indicating a large potential for improvement in production efficiency and output value; Its overall efficiency fluctuates, and there was significant room for improvement in production efficiency; The overall trend of return to scale coefficient was on the rise, and most of them were increasing returns to scale, indicating the need for moderate development of potato scale operations. Based on the current situation of potato production efficiency in the research area, measures to improve quality and efficiency were proposed, including further construction of agricultural infrastructure, promoting full mechanization, accelerating the construction of the industrial chain, and improving social service capabilities, to provided references for the high-quality development of the potato industry.

Sweet potato is an important food crop and cash crop in China, and it has played an important role in ensuring China’s food security. Plant growth regulators not only increase crop yield, but also enhance stress resistance and improve quality, playing an important role in crop production. At present, there are relatively few researches on the application of plant growth regulators in sweet potato. To explore the effects of different plant growth regulators on the agronomic traits of sweet potato seedlings, three plant growth regulators, 6-benzylaminopurine, gibberellin and naphthalene acetic acid, were selected in this study, and their effects on sweet potato seedlings were studied by the method of bottom application. The results showed that the three plant growth regulators significantly promoted the rooting of sweet potato, and significantly promoted the root length and fresh weight of sweet potato. The research results would provide theoretical basis and technical support for further optimization of sweet potato cultivation management, help to improve sweet potato yield and quality, and promote the sustainable development of sweet potato industry.

Sweetpotato root-function spatial division (RFSD) technology is a novel cultivation technique which separates nutrient-absorbing roots from storage roots in space and function. This technology allows for the observation of tuber development without affecting plant growth and enables the study of photosynthetic transport efficiency within the same genetic background. RFSD is a key technique for studying the source-sink relationship in sweetpotato and cultivating overhead-sweetpotato. To further explore the application of RFSD technology in the study of source-sink relationships in sweetpotato, we specifically carried out this review. The paper summarizes the impact of "source", "flow", and "sink" on sweetpotato yield, reveals the development process of sweetpotato RFSD technology, analyzes the principles, evolution, characteristics, and advantages of various RFSD technologies currently developed, and discusses the application of root system functional separation technology in the study of source-sink relationship in sweetpotato and overhead-sweetpotato. Here we propose that RFSD technology can serve as a crucial tool for studying the interactions of "source", "flow", and "sink" in sweetpotato, and be used to investigate source-sink relationship and ideotype within the same genetic background. It is necessary to improve and enhance the RFSD, so as to make it possible to achieve stable storage root in the air under the facility conditions. Further research and application of RFSD technology can provide theoretical and technical references for the breeding of new sweetpotato varieties, especially high photosynthetic efficiency, short-vine, and high-yielding cultivars. It can also offer technical support for facility cultivation and precise quality control.

Based on the current breeding status of potatoes, the aspects that need further improvement, and corresponding development strategies and suggestions were summarized and analyzed. At present, potato breeding was mainly based on its asexual reproduction characteristics, which had the advantages of simplicity, effectiveness, and ensuring the purity of potato seeds. Meanwhile, it was necessary to do a good job in potato seed potato detoxification, pest control, and field management. In the breeding process, it was necessary to further improve the collection of potato variety resources, the production capacity of seed potatoes, the production technology of seed potatoes, the quality of virus-free seed potatoes, and the processing capacity of potato products. Development strategies for potato breeding work were proposed, including establishing a good breeding environment, scientifically introducing potato varieties, innovating potato breeding methods, and innovating production equipment and technology, to provide references for promoting the standardized and rational development of the potato industry.

This research aimed to isolate and identify potato common scab pathogens in soil, and clarify the pathogenic genes and pathogenicity. It is of great significance to carry out breeding and comprehensive prevention and control of scab disease. The collected diseased tubers were identified by tissue separation and coating dilution, and the phylogenetic tree was constructed by 16S rDNA measurement. The pathogenic genes were identified by PCR, and the pathogenicity was tested by radish seedling method, small potato chip method and pot inoculation. Four species of pathogenic Streptomyces were isolated and identified as Streptomyces scabies, Streptomyces thermocarboxydus, Streptomyces rubrogriseus and Streptomyces kanamyceticus. All strains were inoculated with ‘Xisen 3’ for pathogenicity test. The test results showed that four Streptomyces could cause tuber disease, and the pathogenic gene combination was txtAB+/tomA+/nec1+; in this study, a total of 4 pathogenic Streptomyces species were identified. Streptomyces thermocarboxydus, Streptomyces rubrogriseus and Streptomyces kanamyceticus were new pathogens of potato common scab.

Sweetpotato is an important food, fodder and industrial raw material crop in the world. With the rapid development of high-throughput sequencing technologies, transcriptome sequencing technology has been widely used in sweetpotato studies. To better carry out research on functional gene mining and important trait regulation mechanisms analyzing in sweetpotato using transcriptomics techniques in the future, this study analyzed the profile of sweetpotato transcriptome data shared by research institutions in various countries, and summarized the applications and achievements of transcriptome sequencing technology in researches on sweetpotato over recent years, from the aspects of response to adversity stress, tuberous root development and starch synthesis. It was pointed out that there were still deficiencies in experimental design, data mining, and practicality of research results in current sweetpotato transcriptome studies. It was proposed that in further studies, transcriptomics technologies should be integrated with other omics technologies and genetic analysis methods to promote the continuous deepening of sweetpotato research.

The nutritional value, processing technology and product development of potato residue were summarized and analyzed, the relationship between the development and utilization of potato residue and environmental protection was expounded, and the development prospect of potato residue and the aspects to be improved were discussed, so as to provide references for realizing efficient utilization of potato residue resources, green sustainable development and environmental protection. Potato residue is rich in nutrients such as fiber, protein, vitamins, and minerals, making it a valuable resource for various industries. By utilizing advanced processing technologies, potato residue can be converted into high-value products such as feed, biofuels, and biodegradable plastics. These products not only reduce waste and relieve environmental pollution but also contribute to the development of a circular economy. However, there are still challenges to overcome in the efficient utilization of potato residue.,including technical limitations, market constraints, and security. By adopting innovative technologies and strategies, potato residue can be transformed a waste product into a valuable resource, achieving the utilization of agricultural waste resources and environmental protection.

The paper aimed to explore the change characteristics of optimum sowing date and cultivar heat resistance of potato with climate change in North Shanxi based on DSSAT crop, and the yield increase effect of the optimum sowing date or optimum variety was quantitatively analyzed. The simulation experiment was designed with 40 management inputs of sowing date and 7 temperature sensitivity coefficients (TC) under baseline year and future climate change scenarios in Yunzhou of Datong City. The results showed that, in the future, the rising temperature and increase of CO₂ concentration would advance the optimum sowing date of potato, and the change of optimum sowing date was mainly due to the rising temperature. Among them, the optimal sowing date would advance 4 days and 5 days at 450 μmol/mol CO₂ with 1.5℃ warming and 550 μmol/mol CO₂ with 2.0℃ warming, respectively. Adopting the management of optimum sowing date could mitigate climatic negative effects and was in varying degrees of yield increasing effect under each climate change scenario. Among them, the potato yield would increase 10.2% and 20.7% respectively under 450 μmol/mol CO₂ with 1.5℃ warming and 550 μmol/mol CO₂ with 2.0℃ warming. Production at this time would increase the yield by 12.3% and 20.8%, respectively, over the base year. If changing the varieties with high temperature sensitivity, the heat resistance and yield could increase appropriately, but the increase was limited. Therefore, in the future, early sowing date or cultivation of new varieties with high temperature sensitivity can be adopted in northern Shanxi to reduce the adverse impact of future climate change and ensure stable and high yield of potato.

In order to screen and identify biocontrol fungi capable of phosphorus, potassium, and nitrogen fixation in sweet potato stem nematodes, soil samples from the rhizosphere of sweet potato stem nematodes in their habitat was collected. Six bacterial strains, including HC-6 and GS3-X4, were isolated using LB solid medium. Through initial screening, rescreening, and performance testing, a strain with good contact killing effect against nematode disease, strong colonization ability, and excellent phosphorus, potassium, and nitrogen fixation abilities was selected from six strains, which was GS4-S2. Based on physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain was identified as BacillusVelezensis, and named HM-6. This strain could be used as a material for the development of microbial fertilizers for the prevention and control of sweet potato stem nematode disease, which was of great significance for the prevention and control of nematode diseases and the utilization of microbial fertilizers.

Potato Cyst Nematodes (PCNs) are a significant threat to potato production, having caused substantial damage in many countries. Predicting the future distribution of PCN species is crucial to implementing effective biosecurity strategies, especially given the impact of climate change on pest species invasion and distribution. Machine-Learning (ML), specifically ensemble models, has emerged as a powerful tool in predicting species distributions due to its ability to learn and make predictions based on complex data sets. Thus, this research utilised advanced machine learning techniques to predict the distribution of PCN species under climate change conditions, providing the initial element for invasion risk assessment. We first used Global Climate Models to generate homogeneous climate predictors to mitigate the variation among predictors. Then, five machine learning models were employed to build two groups of ensembles, single-algorithm ensembles (ESA) and multi-algorithm ensembles (EMA), and compared their performances. In this research, the EMA did not always perform better than the ESA, and the ESA of Artificial Neural Network gave the highest performance while being cost-effective. Prediction results indicated that the distribution range of PCNs would shift northward with a decrease in tropical zones and an increase in northern latitudes. However, the total area of suitable regions will not change significantly, occupying 16-20% of the total land surface (18% under current conditions). This research alerts policymakers and practitioners to the risk of PCNs’ incursion into new regions. Additionally, this ML process offers the capability to track changes in the distribution of various species and provides scientifically grounded evidence for formulating long-term biosecurity plans for their control. 

马铃薯作为主食材料，富含多种营养物质，对调节居民的膳食营养结构起重要作用。文章综述了基于马铃薯淀粉和马铃薯泥的食品3D打印技术研究，包括马铃薯淀粉作为主材料和添加剂情况下的食品3D打印性能研究，以及食品添加剂和打印温度对食品3D打印性能的影响研究；基于马铃薯泥的食品3D打印技术研究，包括预处理方式、添加剂、打印温度和填充结构对食品3D打印性能的影响研究；并讨论了马铃薯食品3D打印技术面临的问题和发展前景及马铃薯在食品4D打印领域的研究发展方向。

Potassium is one of the essential mineral nutrients for potato growth and development, and is crucial for potato growth and tuber formation. The low efficiency of potassium absorption and utilization is one of the important limiting factors in potato production in China, and screening the potato genotypes with high potassium efficiency is the best way to enhance the potassium utilization and deal with low potassium stress in potato. In this paper, the methods and indexes for screening of high potassium efficiency varieties of various crops in China and abroad were summarized from four aspects: the definition and assessment of potassium efficiency, the identification and evaluation of crop genotypes with high potassium efficiency, the physiological basis of high potassium absorption and the physiological basis of high potassium utilization. Combining with the characteristics of potato potassium requirement, the feasible methods and influencing factors for screening potato genotypes with high potassium efficiency were put forward, and the future research direction for improving potato potassium efficiency was prospected so as to provide a reference for the high yield and high potassium utilization of potato.

针对黏重黑土地区马铃薯挖掘铲挖掘阻力大、能耗高等问题，以白茅根膜质叶鞘为仿生原型，设计了一种基于仿生波纹结构的马铃薯减阻挖掘铲。基于离散元（DEM）法仿真和土槽试验，确定减阻性能最优的设计参数；通过田间试验以油耗和阻力为指标验证仿生挖掘铲的减阻效果和挖掘性能。离散元仿真得到仿生挖掘铲最佳布置方式为纵向布置；在相同纵向布置方式下，通过土槽试验得到仿生挖掘铲的波纹参数在幅值为2.5 mm、频率为0.5时表现出较为优异的减阻性能。根据最佳参数研制仿生纵波纹铲，进行普通平铲和仿生纵波纹铲的作业对比试验，结果表明仿生纵波纹铲阻力减少了14.45%，单位油耗减少了17.15%。研究表明仿生纵波纹铲具有较好的减阻特性，仿生结构设计合理，能实现黏重黑土条件下马铃薯收获挖掘作业，可为整机节能减耗的研究奠定基础。

芽眼精准检测是实现马铃薯种薯智能化切块的前提，但由于种薯芽眼区域所占面积小、可提取特征少以及种薯表面背景复杂等问题极易导致芽眼检测精度不高。为实现种薯芽眼精准检测，该研究提出一种基于改进YOLOv7的马铃薯种薯芽眼检测模型。首先在Backbone部分增加Contextual Transformer自注意力机制，通过赋予芽眼区域与背景区域不同权值大小，提升网络对芽眼的关注度并剔除冗余的背景信息；其次在Head部分利用InceptionNeXt模块替换原ELAN-H模块，减少因网络深度增加而造成芽眼高维特征信息的丢失，更好地进行多尺度融合提升芽眼的检测效果；最后更改边界框损失函数为NWD，降低损失值，加快网络模型的收敛速度。经试验，改进后的YOLOv7网络模型平均准确率均值达到95.40%，较原始模型提高4.2个百分点。与同类目标检测模型Faster-RCNN(ResNet50)、Faster-RCNN(VGG)、SSD、YOLOv3、YOLOv4、YOLOv5n、YOLOX相比，其检测精度分别高出34.09、26.32、27.25、22.88、35.92、17.23和15.70个百分点。在马铃薯种薯自动切块试验台上进行芽眼检测试验，对于表面光洁及表面附有泥土、破损的马铃薯种薯，改进后模型的漏检率分别为4%、11%，检测效果优于其他网络模型。研究结果可为后续马铃薯种薯智能化切块芽眼检测提供技术支持。

[目的]解决基于人工特征设计分级方法精度低、鲁棒性差的问题。[方法]提出了一种强泛化的Transformer薯形与大小自动分级方法。基于Transformer模型构建2个PotatoViT模型，并完成马铃薯薯形分级和大小分级任务；利用迁移策略和数据增广方法训练出鲁棒性分级模型；通过测试集定量分析，验证了研究所提方法在马铃薯分级中的有效性。[结果]PotatoViT模型对薯形分级的准确率和模型F₁得分分别为96.36%,94.75%,对大小分级的准确率和模型F₁得分分别为89.66%,85.16%,分级精度优于VGG16、ResNet50和MobileNetV3网络模型。[结论]研究所提方法对马铃薯薯形与大小的准确、实时检测是可行的。

本研究通过大田试验,分别设置0（处理1）、900（处理2）、1 050（处理3）、1 200（处理4）和1 350 kg/hm² （处理5）共5个施肥处理,探讨不同施用量复合肥对马铃薯产量及疮痂病发病情况的影响。结果表明,产量方面,处理2（43 928.6 kg/hm²）,处理4（42 023.8 kg/hm²）和处理3（41 277.8 kg/hm²）产量较高,与处理1差异存在统计学意义（P<0.05）,但处理间差异无统计学意义（P>0.05）;病薯率方面,处理1的病薯率最高（98.1%）,处理4病薯率最低（91.9%）,各处理间差异无统计学意义（P>0.05）;病情指数方面,处理1的病情指数最高（56.6）,与其他处理间差异具有统计学意义（P<0.01）,其他处理间病情指数差异无统计学意义;防效方面,不同施肥处理的防效差异无统计学意义（P>0.05）。综合各结果来看,处理2、处理3和处理4的表现较好,其疮痂病发病较轻,产量较高,兼顾了病害防治和经济效益,因此,建议生产上该作物复合肥施肥量以900~1 200 kg/hm²为宜。

Potato is an important food crop and is very sensitive to high temperature. In order to study the effects of high temperature stress on the physiological characteristics and yield of potato leaves, ‘Qingshu 9’was used as the experimental material, and the leaf area, chlorophyll content, photosynthetic fluorescence characteristics and yield of potato were systematically studied using randomized block design. The results showed that the leaf area index and leaf SPAD value decreased significantly under high temperature stress. The leaf area indexes of T₁ treatment were decreased by 11%-34% compared with T₂ treatment, and they were decreased by 34%-68% compared with T₃ treatment. The SPAD value of T₂ treatment was decreased by 2.43% compared with T₃ treatment. Secondly, the photosynthetic fluorescence and yield also decreased, and the recovery was relatively slow, which inhibited the production of Pn and the absorption and transformation of light energy. The reactive oxygen species increased, and the leaves were dehydrated and wilted to cause death, which would affect the whole growth process of potato, resulting in the final reduction of yield. Therefore, high temperature will lead to the change of functional leaves, which will reduce the photosynthetic performance of potato, gradually shorten the active period, disorder the physiological metabolic activity, and significantly reduce the yield.

In order to explore the overwintering plug seedlings of leaf-vegetable sweet potato in North China, 4 stem sizes (D1, D2, D3, D4 for 4, 6, 8, 10 cm) and 5 plug sizes (32, 50, 72, 105, 128 holes) were set to match 14 treatments, to study the effects of different combinations on seedling survival rate, growth (stem diameter, plant height, etc.) and fresh weight, etc. The results indicated that under the same plug, with the increase of stem segment, the survival rate of seedlings, the number of leaves, stem diameter of the base region and plant height of seedlings showed an increasing trend, and then the fresh weight of the aboveground part and the whole plant also increased, and the highest value was under the treatment of D4. Under the same stem segment, as the number of holes in the plug increased, the number of leaves, stem diameter of the base region, plant height, fresh weight above the ground and fresh weight of the whole plant decreased, due to the effect of the plug size on the underground part, with the treatment of 32 holes being the highest. Under different stem segments and plug sizes, the growth of D4-32 treatment was the best. In mid-March, for the fresh weight of the aboveground part, the treatment of D2-72 achieved the same effect as the treatment of D4-32(no significant difference between the two treatments), with the number of leaves, stem diameter of the base region, plant height and other indexs of seedlings meeting the transplanting conditions; for the fresh weight of the whole plant, the treatment of D2-105 treatment (10.21 g) achieved the same effect as the treatment of D4-32, which could save 228.1% of the seedling area. According to its weight, in mid-January, the fresh weight of the aboveground part and the whole plant under the treatment of D3-72 was close to the level of the above two treatments, which could save 125.0% of the seedling area. Considering the growth and economic benefits of seedlings, D2-105 is recommended when transplanted in mid-March, that is, the combination of stem segment with 2 axillary buds and 105-hole plug. If there is condition for transplanting in mid-January, D3-72 is recommended, that is, the combination of stem segment with 3 axillary buds and 72-hole plug.

【Objective】 Jasmonic acid (JA) is one of key regulatory hormones during potato tuber development. Study on JA regulating tuber development mechanism would provide an important theoretical basis for tuber yield and quality formation.【Method】 In this study, potato stolons cultured in vitro were exogenously applied with JA (0, 0.5, 5, 50 μmol·L^-1) to analyze the phenotype, tissue microstructure, carbohydrate accumulation and proteome change of tubers.【Result】 With the increase of JA concentration, the tuber number formed per stolon, tuber diameter, fresh and dry weight, cell area of perimedullary zone, starch and soluble sugar content were gradually increased at 0.5 and 5 μmol·L^-1 JA (P<0.05), whereas the tuber diameter, fresh and dry weight, starch content were significantly decreased at 50 μmol·L^-1 JA (P<0.05). Lipoxygenase activity was gradually decreased with JA concentration increasing (P<0.05). Thirty-five differentially abundant proteins (P<0.05 and fold change≥2.5) closely related to tuber development regulating by JA were identified by 2-DE and MALDI-TOF/TOF-MS. They were mainly involved in bioenergy and metabolism (28.6%), cell defense and rescue (28.6%), protein biogenesis and storage (11.4%), signaling (8.6%), transcription (8.6%), unknown (8.6%) and miscellaneous (5.6%). The differential expression patterns of these proteins were clustered into three categories by hierarchical clustering analysis. Cluster 1 included 17 proteins that down-regulated at 0.5 μmol·L^-1 JA and up-regulated at 5 μmol·L^-1 JA, which mainly involved in bioenergy and metabolism, protein biogenesis and storage, signaling and transcription. Cluster 2 included 10 proteins that gradually up-regulated with JA concentration increasing, which were mainly involved in cell defense and rescue, bioenergy and metabolism, transcription. Cluster 3 included 8 proteins that down-regulated after JA treatments, which were mainly involved in bioenergy and metabolism, cell defense and rescue, protein biogenesis and storage. 【Conclusion】 Low JA concentration (0.5, 5 μmol·L^-1) might promote tuber morphogenesis by inducing cell enlargement in tuber perimedullary zone, intracellular sucrose and polysaccharide accumulation, and cell defense ability, whereas high JA concentration (50 μmol·L^-1) showed inhibitory effects.

The paper aims to investigate the effects of potato onion on tomato fruit quality and soil nutrients. Using ‘Dongnong 708’ tomato as the experimental material, a pot experiment was conducted, with two treatments of monoculture tomato and potato onion associated tomato. Rhizosphere soil samples at 20, 30, 40 and 100 days after sowing were collected, and the quality of the first and second ear fruits of tomatoes after fruit ripening was analyzed. The results showed that the content of vitamin C, soluble sugar, sugar acid ratio, soluble protein, and lycopene in the accompanying tomato fruits of potato onions was significantly higher than that of monoculture tomatoes. The titratable acid content of tomato fruits was significantly lower than that of monoculture tomatoes, and the nutritional quality of the first ear fruit was better than that of the second ear fruit, while the taste quality of the second ear fruit was also better than that of the first ear fruit. The pH, EC, available phosphorus, available potassium, acid hydrolyzed phosphorus, acid hydrolyzed nitrogen, and organic matter concentration in the rhizosphere soil of tomato associated with potato onion were significantly higher than those of monoculture treatment, while the content of nitrate nitrogen and ammonium nitrogen was significantly lower than that of monoculture treatment. In summary, potato onions can increase the activity of soil nutrients, promote nutrient absorption by tomatoes, providing sufficient nutrient foundation for tomato growth, and thus improve tomato quality.

To clarify the effect of replacing chemical fertilizers with organic fertilizers on soil and potato, explore the impact on soil nutrient content, soil enzyme activity and quality of potato, and propose a suitable organic substitution ratio, so as to provide scientific basis for the promotion of replacing chemical fertilizers with organic fertilizers. In Xiji County, Guyuan City of Ningxia in 2022, five treatments, CK (single fertilizer), T₁ (organic alternative fertilizer 20%), T₂ (organic alternative chemical fertilizer 40%), T₃ (organic alternative chemical fertilizer 60%) and T₄ (single application of organic fertilizer), were set up to analyze the relationship between soil nutrient content, soil enzyme activity and quality of potato using the ‘Qingshu 9’ potato variety as the test material. The results showed that (1) compared with CK and T₄, the contents of alkaline-hydrolyzable nitrogen, available phosphorus and available potassium of other treatments were increased to varying degrees, and the effect of increasing the content of available potassium was the most significant, increasing by 13.86%-37.74% and 2.55%-42.10%, respectively. (2) The soil enzyme activity of soil depth of 0-20 cm was slightly higher than that of 20-40 cm, and compared with CK and T₄, the soil enzyme activity of organic fertilizer of other treatments was improved, and the index of alkaline phosphatase activity was the highest, compared with CK, 0-20 and 20-40 cm increased by 3.74%-56.11%, 2.02%-55.90%, respectively; compared with T₄ treatment, it increased by 7.57%-64.94% and 8.56%-50.57%. (3) Compared with CK and T₄ treatment, organic fertilizer replacing partial chemical fertilizer treatments improved the quality content of potato tubers. (4) Correlation analysis showed that soil enzyme activity was positively correlated with soil nutrient content, and the increase of soil nutrient content promoted the improvement of enzyme activity. The stepwise linear regression equations of soil nutrient content, enzyme activity and potato quality showed that alkaline hydrolyzable nitrogen, urease, alkaline phosphatase and 20-40 cm catalase activities could be explained by the correlation with potato quality. The replacement of partial chemical fertilizers by organic fertilizer could increase soil nutrient content, enhance soil enzyme activity and improve potato quality, among which the replacement of 40% chemical fertilizer by organic fertilizer had the most significant effect.

【Objective】 The selection of low glycemic index potato varieties is not only essential for controlling blood glucose, reducing obesity, and maintaining oral health but also constitutes a crucial approach to meeting diverse consumer demands and enhancing potato production efficiency. This process provides a foundation for the breeding of low glycemic index potato varieties and the improvement of biological breeding methods.【Method】 Eight domestically and internationally cultivated potato varieties were employed as experimental materials. The study involved the examination of tuber agronomic traits, analysis of total starch, amylose, rapidly digestible starch, slowly digestible starch, resistant starch, soluble sugars, insoluble dietary fiber, soluble dietary fiber, and soluble protein content in tubers before and after steaming processing. Additionally, the investigation included the evaluation of the retention levels of these components and the post-processing tuber flavor quality and in vitro/vivo glycemic index.【Result】 Among the eight varieties, the yield ranged from 21.50 to 49.90 t·hm^-2, with marketable yield percentages ranging from 60.04% to 90.21% and length-to-width ratios from 1.21 to 2.90. Sensory evaluation scores for flavor ranged from 64 to 73. Texture profile analysis results indicated that hardness ranged from 9.78 N to 19.97 N, adhesiveness from 0.44 mJ to 1.66 mJ, cohesiveness from 0.052 to 0.070, springiness from 0.51 to 1.02 mm, and chewiness from 0.28 to 1.38 mJ. Before and after steaming, the total starch content of eight potato varieties ranged from 67.07% to 76.72% dry weight (DW) and 57.69% to 67.40% DW, respectively. The range of amylose content was 5.36% to 19.23% DW and 5.43% to 6.83% DW, while rapidly digestible starch content varied from 1.18% to 8.23% DW and 14.31% to 28.56% DW. The range of slowly digestible starch content was 3.33% to 7.69% DW and 12.81% to 27.65% DW, and resistant starch content varied from 53.71% to 70.36% DW and 11.80% to 25.80% DW. Soluble sugar content ranged from 25.98 to 56.86 mg·g^-1 DW and 11.38 to 50.24 mg·g^-1 DW, while total dietary fiber content varied from 29.62% to 36.17% DW and 43.67% to 52.55% DW. Insoluble dietary fiber content ranged from 17.69% to 23.70% DW and 30.31% to 44.12% DW, and soluble dietary fiber content ranged from 11.07% to 18.48% DW and 7.37% to 14.09% DW. Soluble protein content varied from 42.26 to 64.14 mg·g^-1 DW and 0.71 to 4.82 mg·g^-1 DW. Following steaming, the total starch content of the eight varieties exhibited a range of -15.49% to -5.97%, with changes in amylose content ranging from -12.39% to 0.56%. The variations in rapidly digestible starch, slowly digestible starch, and resistant starch were in the ranges of 10.44% to 25.86%, 5.12% to 23.09%, and -56.8% to -29.88%, respectively. Soluble sugar content varied from -27.07% to 15.70%, while changes in insoluble dietary fiber and soluble dietary fiber ranged from 11.41% to 25.19% and -4.73% to 0.77%, respectively. Soluble protein content exhibited a range of -60.86% to -39.67%. Correlation analysis revealed a significant positive correlation between the glycemic index and tuber total starch and rapidly digestible starch content, while a significant negative correlation was observed with resistant starch and insoluble dietary fiber content. The glycemic index of the eight varieties ranged from 58.08 to 100.64 in vitro and from 57.80 to 92.47 in vivo【Conclusion】 Under potato breeding program, the in vitro glycemic index can replace the in vivo glycemic index as an alternative evaluation method. The content of tuber total starch, rapidly digestible starch, slowly digestible starch, resistant starch, and insoluble dietary fiber are key agronomic traits be considered in the breeding process of low glycemic index potato varieties. The Lucinda was identified as a low glycemic index potato variety with superior flavor quality after cooking processing.

In order to clarify the types of pathogens causing potato common scab in the micro potato continuous cultivation substrate, organic acids with good inhibitory activity were screened to control this disease. In this study, pathogenic bacteria were isolated and purified from diseased potato, and their pathogenicity was detected by potato chip method, radish chip method and greenhouse pot inoculation method. The species were determined by morphological observation, physiological and biochemical characteristics determination and 16S rRNA gene sequence analysis. The biological characteristics such as salt tolerance were studied, the effects of organic acids such as formic acid on the growth of Streptomyces spp. were determined using paper plate method. Streptomyces strain 19311 was pathogenic, and its pathogenic island virulence related genotype was txtAB⁺/tomA⁺/necI⁺. Strain 19311 was identified as Streptomyces acidiscabies based on the morphological, physiological and biochemical characteristics and 16S rRNA sequence analysis. Formic acid, acetic acid, propionic acid, lactic acid, citric acid, and tartaric acid could significantly inhibit the growth of S. acidiscabies, and formic acid had the best antibacterial effect with the antibacterial zone diameter of 45.43 mm and the lowest effective concentration of 1%, which could inhibit the growth of S. acidiscabies by increasing the permeability of cell membrane. In this study, the species and biological characteristics of Streptomyces strain 19311 in the micro potato cultivation substrate were identified. The use of 1% formic acid to control the occurrence of this disease and evaluate its impact on the original seed production can be carried out in the future.