Saline-alkali stress is one of the abiotic stresses in the process of plant growth and development, which can cause water deficit, changes in cell membrane permeability, metabolic disorders and blockage of protein synthesis in plants, resulting in crop yield reduction or death. Finding effective methods to reduce the harm of saline-alkali stress and strategies to improve the saline tolerance of plants are of great significance to the comprehensive utilization of saline land. In this paper, the latest researches on the damage and adaptive mechanism of plants under saline-alkali stress in recent years were summarized, and the physiological and molecular mechanisms of plants responding to saline-alkali stress were summed up. Furthermore, the physiological mechanisms of plants under saline-alkali stress were analyzed, which were mainly regulated by accumulating osmotic substances, increasing the activity of antioxidant enzymes and ionic compartmentalization, etc., and the molecular mechanisms were mainly regulated by signal transduction, transcription factor regulation and the expression of plant salt-tolerance-related gene, etc. This study pointed out the trends and urgent problems in the development of plant adaptation to saline-alkali environments, with a view to providing a certain theoretical basis for the selection and cultivation of saline and alkali tolerant plants.

The genus Polygonatum Mill, commonly referred to as "Huang Jing," are plants renowned for their medicinal and edible properties. Their active constituents, including polysaccharides, flavonoids, and saponins, exhibit notable pharmacological effects. Recent studies have predominantly centered on the extraction of saponin components from the genus Polygonatum, which are attributed with anti-fatigue, antioxidant, hypoglycemic, lipid-lowering, and immune-enhancing properties. While there has been some advancement in the structural analysis of the genus Polygonatum saponins, their biosynthetic pathways remain underexplored. The synthesis of saponins in the genus Polygonatum occurs via the mevalonic acid pathway and the 2-C-methyl-D-erythritol-4-phosphate pathway, encompassing multiple enzymatic steps. This research indicates that the genus Polygonatum saponins exert diverse biological effects, including anti-tumor, antibacterial, anti-inflammatory, antiviral, and immune regulatory activities, and hold significant potential in medicine, food, cosmetics, and other sectors. Nonetheless, current studies are limited by inadequate structural analysis, an incomplete understanding of biosynthetic pathways, and ambiguous mechanisms of pharmacological action. Future endeavors should prioritize the isolation, structural analysis, synthetic biology, and pharmacology of the genus Polygonatum saponins to advance their scientific investigation and product development, thereby laying a foundation for the creation of novel therapeutic agents.

In order to effectively control Huanglongbing (HLB) and reduce the loss of HLB to the global citrus industry, this study reviewed the latest research on the control of HLB in recent years. The results showed that there were no effective agents and disease-resistant varieties. At present, the main methods of controlling HLB include traditional ‘three fast methods’, namely planting disease-free seedlings to control HLB from the sources, preventing and controlling Diaphorina citr to block its transmission path, and digging infected trees to eliminate the infection source; expanded physical control technologies include seedling virus-free, light, steam heat treatment, etc.; multi-directional chemical drug control technologies include antibiotics, nanomaterials, immune inducers and antimicrobial peptides; emerging biological control technologies include various probiotics, microbial preparations, etc. Finally, the application prospect of soil amendment and biological control agent combined treatment of HLB and citrus transgenic disease resistance technology based on genetic improvement was prospected, to provide theoretical basis and practical reference for effective control of HLB.

An important goal of soil management in the Songnen Plain is improvement of soda saline-alkali soil. Summing up the previous research results on soil improvement, this article summarized the application types, action mechanisms, improvement effects, existing problems of improvers in soil improvement, and looked forward to the application prospects of improvers, in order to provide reference for future in-depth research. The results showed that improvers could be divided into inorganic improvers, organic improvers, and microbial agents. The use of combined improvers was better than that of single improver. The application of improvers improved soil physical and chemical properties, structure, nutrient content, and micro ecological environment, and promote plant growth. The research and development of new organic improvers were the focus of future research.

China faces significant challenges of large population and limited arable land resources. In recent years, extreme weather events and ongoing soil degradation have significantly influenced China's agricultural production. With advances in agricultural science and technology, incorporating new materials into agricultural practices has been proven to be an effective strategy for adapting to these developments and environmental shifts. Seaweed extract, specifically seaweed oligosaccharides, are natural active substances derived from seaweed using modern technology. They are employed in agriculture as inducers for plant stress and disease resistance, biostimulants, and fertilizer enhancers. With continuous research progresses on the potential applications of seaweed oligosaccharides in agriculture, significant advancements have been achieved in their study and use. To further systematically understand and apply seaweed oligosaccharides, this paper discussed their sources, classification, and production methods. It summarized their impact on bolstering crop stress and disease resistance, fostering growth and development, and improving nutrient absorption, as well as their effectiveness and mechanisms across various crops. This provided innovative approaches and tools for the sustainable and efficient development of Chinese agriculture in the current context.

Vermicomposting is a simple, feasible, low-cost and environmentally friendly bio-microbial coupled organic waste recycling technology. The physicochemical and microbial characteristics of vermicomposting in the treatment of organic waste were summarized. The application research progress of vermicomposting in agricultural production was introduced, and the following suggestions were proposed in view of the problems existing in the practical application of vermicomposting. (1) In order to develop and produce high quality commercial earthworm manure, it is suggested to use the relevant factors affecting the quality of earthworm compost products to carry out mathematical modeling of vermicomposting, so as to efficiently optimize the parameters of vermicomposting, reduce research and development costs and improve production efficiency for large-scale production of earthworm manure; (2) in order to ensure the stable output of high quality earthworm manure, equipment with intelligent control of pH, carbon to nitrogen ratio, temperature and humidity and light should be developed to form automatic and large-scale vermiculture; (3) in order to promote the development of the circular economy of "planting and breeding integration", the effective parts containing humic acid in the process of culturing earthworms can be reused, and the efficiency of vermicompost utilization in agricultural planting and soil remediation should be strengthened.

The global warming rate and the frequency of extreme high temperature weather are continuously increasing, which could bring catastrophic impacts to rice production. To further clarify the response mechanism of rice to high temperature, this article summarized the research progress in the direction of rice heat damage from high temperature, focused on the impact of high temperature on rice production, and deeply analyzed the physiological (photosynthetic characteristics/antioxidant system) and gene molecular mechanisms of rice’s response to high temperature. We concluded that the heat tolerance characteristics of rice were formed by the interaction between varieties and the environment. Results showed that, the photosynthetic characteristics of rice were influenced by both stomatal and non-stomatal limiting factors, and the antioxidant process was achieved by enhancing the activity of antioxidant enzymes and reducing the content of malondialdehyde; rice improved plant heat adaptability by activating the expression of key genes and stimulating the transduction of heat signals within the plant; by optimizing “Before production-During production-After production” comprehensive management and monitoring system, we could explore innovative cultivation systems, and thoroughly investigate the high temperature defense mechanisms of rice. Therefore, this study aimed to clarify the physiological and molecular mechanisms of rice in response to high temperature stress, providing a scientific reference for future heat-tolerant rice breeding work and improving rice sustainable production.

To explore the growth differences among different green manure varieties and their effects on rice yield, nutrient accumulation and soil properties after turning over, the winter fallow field (CK) was used as the control, three common green manures (Astragalus sinicus L., Vicia villosa var. and Vicia sativa L.) were selected to conduct field experiments to analyze the differences in the characteristics of green manures during the growth period and their effects on rice yield, nutrient accumulation and soil properties after turning over. The results showed that the performance of plant height and biomass in the three types of green manure was as followed: Vicia villosa var. > Vicia sativa L. > Astragalus sinicus L., with Vicia villosa var. being the best and suitable for planting in the region. After turning over green manures, the rice yield significantly increased compared to CK, with the yield under Vicia villosa var. reached 10.47 t/hm², which was 58.88% higher than that of CK. Green manure turning over could improve the taste value and nutrient accumulation of rice. Compared with CK, the taste value of rice treated with Astragalus sinicus L., Vicia villosa var. and Vicia sativa L. increased by 5.58%, 9.46% and 11.16%, respectively, and total potassium accumulation in rice straw increased by 50.88%, 42.87% and 67.70%, respectively, the total nitrogen accumulation in rice grains increased by 17.25%, 45.62% and 47.74%, respectively and the total potassium accumulation in rice seeds increased by 17.96%, 54.28%, and 48.88%, respectively, the total phosphorus accumulation in rice shoots increased by 11.32%, 62.16% and 27.41%, respectively, the total accumulation of total potassium increased by 45.53%, 44.73% and 64.64%, respectively, with the best comprehensive effect being the Vicia villosa var. and Vicia sativa L.. Green manure could improve soil properties and had the potential to improve soil fertility. Among them, the treatment of Vicia sativa L. had a pH increase of 2.96%, organic matter increased by 10.05%, and total nitrogen content increased by 4.41% compared to CK. The treatment of Astragalus sinicus L. had an organic matter increase of 1.43%, total nitrogen content increase of 24.25%, total phosphorus content increase of 9.20%, and available phosphorus content increase of 10.95% compared to CK. In summary, the biomass and nutrient accumulation of Vicia villosa var. and Vicia sativa L. were both high, and their flipping could improve the yield, taste value and nutrient accumulation of rice. Therefore, they were recommended as green manure varieties for planting in this region; Astragalus sinicus L. and Vicia sativa L. had the potential to increase soil fertility through tillage, while Vicia sativa L. had a better effect. This study could provide a theoretical basis for the utilization of green manure and sustainable agricultural production in the northern Jiangsu region.

Fusarium oxysporum (Fo) is a soil-borne fungal disease that causes crop wilting, which is a serious constraint to the sustainable development of agriculture. Effectors are key factors in pathogen-plant interactions, and their mechanism of action can be analyzed to provide effective strategies for controlling plant diseases. When Fo infects crops, it secretes a protein in the xylem of vascular bundles, which is called Secreted In Xylem effector protein (SIX). In order to study the role of SIX effectors between Fo and plants, the structural characteristics of SIX on the Fo genome were analyzed. The functional attributes of SIX in identifying different formae speciales and physiological races of Fo, influencing the pathogenicity of Fo, and affecting the immune response of plants were summarized. It was pointed out that SIX had toxic effects on plants and could interact with plants in an incompatible manner to influence the invasion of pathogens. The results of this study reveal the effects of SIX on plants in different aspects and provide theoretical support for the study of the molecular mechanism of plant disease resistance and the selection and breeding of disease-resistant varieties.

Hyperspectral remote sensing is an important technology for crop research. In order to promote the healthy development and basic research of crop growth cycle, improve the application level of remote sensing technology in crop research, this paper summarizes the analysis algorithms of hyperspectral in crop research, focus on the research progress of hyperspectral in crop growth, crop information monitoring, and crop information extraction. This paper systematically reviews the studies on leaf area index, biomass estimation, nitrogen monitoring, chlorophyll monitoring, pest and disease monitoring, heavy metal monitoring, crop carbon to nitrogen ratio, and water content, which still need to be further developed. Analysis shows that the combination of hyperspectral technology and artificial intelligence has achieved initial results in crop research which is still difficult to meet the intelligent needs of modern agriculture. If the new generation of information technology and large models are used to empower, enormous research potential will be unleashed.

In order to explore the stress of water environment changes on cultured fish in the development of intensive aquaculture, the effects of environmental factors such as temperature, pH, dissolved oxygen, ammonia nitrogen and heavy metals on fish body were studied. From the five aspects of growth performance, blood biochemistry, digestive enzyme activity, antioxidant capacity and immunity, the related mechanism of environmental stress factors affecting fish health was reviewed, and the importance of environmental stress factor research was revealed. It was suggested that comprehensive methods such as field investigation, long-term monitoring and calculation models should be more widely used in the future to comprehensively explore and evaluate the coping strategies and health status of fish in the actual environment, and to explore the molecular mechanism of environmental stress factors on fish health. Future experiments need to study the whole process of fish growth in different periods, which is helpful to predict the impact of future environmental changes on fish community structure and ecosystem function. By studying the adverse effects of environmental stress factors on fish health, the occurrence of stress can be avoided or weakened, which provides a reference for ensuring efficient and healthy aquaculture of fish.

The flavor substances of cigar tobacco leaves play a decisive role in the stylistic characteristics of cigars. Fermentation is an important process to improve the quality of cigar tobacco, which can affect the type, content and composition ratio of flavor substances in cigar tobacco, thus determining the quality of cigar tobacco leaves and cigar tobacco quality. This paper reviews the mechanism of fermentation and flavor perception of cigar tobacco leaves and the progress of flavor analysis of cigar tobacco leaves under different fermentation conditions, summarizes their effects on cigar tobacco leaves, and looks forward to the future research on cigar flavors to provide theoretical support for related research.

Long-term addition of antibiotics in feed can cause the problems such as drug residues and bacterial resistance, leading to a decline in the quality of livestock and poultry products, threats to human health, and damage to the ecological environment. In 2020, Announcement No. 194 of the Ministry of Agriculture and Rural Affairs officially implemented, and the use of growth promoting antibiotic additives in feed was completely prohibited, marking the arrival of the era of "anti antibiotic" in the livestock and poultry industry. Non antibiotic breeding has become a major trend in the development of the livestock and poultry industry. Antibiotic substitutes can improve the growth performance of animals, improve intestinal health, and regulate the host immune system. They have the advantages of safety, efficiency, low residue, and low pollution, and have great prospects in the application of feed additives. Therefore, this article reviews the research progress in the application and action mechanism of green additives such as antibiotic substitutes, microecological agents, organic acids, plant extracts, Chinese herbal medicines, antimicrobial peptides, etc., in order to provide reference for healthy livestock breeding and promote the green and healthy development of livestock and poultry breeding in China.

Theaflavins are the main components of black tea, which have great potential and promising applications in the fields of foods and medicines. Base on the synthesis mechanism of theaflavin, the advance of the main preparation methods of theaflavin was summerized, such as direct extraction and preparation in vitro which can be divided into chemical oxidation method, enzymatic oxidation method and biomimetic synthesis method. The primal problem of the above synthesis methods, such as the inability to achieve industrialization, were also addressed, possible future research fields were reviewed in this paper, with the aim of providing a reference for the research and development of preparation of theaflavins.

The microbial composition and function in the rhizosphere is the research focus of microbial ecology. Analysis of the diversity of soil microbial communities in the root system of paddy rice and the effects of environmental factors on soil microorganisms can provide reference value for the sustainability of soils in the growing areas of paddy rice and the conservation of land resources. In this study, we used 16S rRNA high-throughput sequencing technology to determine the bacterial composition of soil microorganisms in the rhizosphere of paddy rice, and analyze the composition and diversity of microbial communities sampled from different regions, and explore the relationship between soil microbial diversity and soil physicochemical properties. The results showed that a total of 33862 OTUs, belonging to 38 phylums, 88 orders, 214 orders, 514 families and 2268 genera, were obtained from the paddy rice rhizosphere soil samples collected from the four regions by sequencing. At the phylum level, the dominant phyla of paddy rice rhizosphere soil bacteria collected from the four regions were Proteobacteria, Bacteroidetes and Firmicutes. At the genus level, Limisphaera was the dominant taxon of paddy rice rhizosphere soil bacteria. As shown by the Alpha diversity index, the species diversity and richness of paddy rice inter-root soil bacteria collected from the four regions were generally as followed: 68^th Regiment of the Corps (D)> Dairy Farm of Yili Prefecture (A)> Chabchal Town (B)> Sundzach Niuzhu Township (C); the Beta diversity index indicated that the paddy rice rhizosphere soil collected from the four regions differed accordingly in their bacterial community compositions, with greater differences existed between group A and other groups. The correlation analysis between soil bacteria and environmental factors concluded that the main influencing factors on the composition of microbial bacterial communities in paddy rice rhizosphere soils might be nitrogen, total potassium, and organic carbon. This study clarified the rhizosphere soil conditions and rhizosphere soil bacterial community characteristics of paddy rice distributed in the Ili River Valley, and provided theoretical support for the study of soil ecosystems in the Ili River Valley.

Bacillus thuringiensis (Bt) is a widely distributed Gram-positive bacterium that can produce a series of insecticidal proteins during its vegetative growth and sporulation phases. It is commonly used as a biocontrol agent in agriculture, forestry and public health practice. The Bt crops developed based on genetic engineering technology provide an efficient and environmentally friendly biological control method for agricultural pest management. However, the extensive and prolonged cultivation of Bt crops has led to the gradual development of Bt resistance in pests, which has greatly reduced the insecticidal effect of Bt proteins and the long-term benefits of Bt crops. In order to gain a deeper understanding of the relationship between the mechanism of action of Bt proteins and the occurrence of Bt resistance in insects, this paper summarized the structural and functional characteristics of various Bt toxins, and elaborated the molecular mechanism of Bt resistance in insects from three main aspects: immune system regulation, changes in toxin activation, and mutations in toxin receptor genes. In addition, two insect resistance management strategies, gene stacking and ‘high dose/shelter’ were also introduced. Finally, the future research directions of Bt crops were pointed out, including further in-depth analysis of the mode of action of Bt toxins and the mechanism of Bt resistance in insects, exploration of new Bt proteins, strengthening the popularization and publicity of Bt crops, and establishing resistance monitoring network as well as early warning system.

Fish blood cells are important part in fish body immunity. They participate in many physiological activities, such as body phagocytosis, secretion, damage repair, defense and others. They are sensitive to changes in their own physiological state as well as to the stimulation of external environmental factors. Blood cells play an important role in indicating and maintaining the physiological homeostasis of fish. Based on recent research reports of domestic and international developments, this paper investigated the progress of fish blood, blood cells and their immune defense functions, and reviewed the blood composition, blood cell genesis, differentiation and related immune defense mechanisms of fish. This review is helpful to deepen the understanding of fish blood immune defense system and its related molecular mechanism, and provides references for further exploration of molecular markers related to fish blood immune defense and screening of molecular phenotypes of fish disease and stress resistance.

The rapid and real-time acquisition of soil moisture in a large range can provide powerful data support for dealing with drought. In this paper, three drought index models, namely perpendicular drought index (PDI), modified perpendicular drought index (MPDI) and temperature vegetation dryness index (TVDI), were constructed to discuss the accuracy and applicability of soil moisture inversion in the winter wheat planting area in Dingxing and Yixian of Hebei based on multi-spectral remote sensing data Landsat-8 and field measured soil moisture data. Three drought index models indicated that the study area was dry, and the spatial distribution of different drought indices was different, among which the difference between PDI and the other two drought indices was the biggest. All the three drought indices were negatively correlated with measured soil moisture, and MPDI, TVDI had obvious linear correlation with soil moisture. MPDI had the highest fitting degree and was used to invert the soil moisture. The inversion results showed that the soil moisture in the study area was relatively low, mainly ranging from 12% to 15%, and the spatial distribution characteristics of soil moisture were consistent with that of land cover. Our study indicated that the MPDI index had great potential for drought monitoring in the winter wheat planting area in winter.

In this paper, the research status of edible dock was briefly described from the aspects of nutritional value, safety, extraction technology of protein and active substances, food and health products, feeding, soil improvement and cultivation technology, etc. It was analyzed that the values of nutrition, health care, food use, feeding use and soil improvement of edible dock were high, but people’s recognition of leaf-eating grass was low, the related basic and applied technology research was still in the initial stage, and the development of the industry was slow. Three suggestions were put forward to promote the research and industrial development of edible dock, to improve people’s recognition of edible dock through propaganda and production of high-value products of edible dock; accelerate the research on the introduction and cultivation of edible dock, and promote intensive and large-scale planting; strengthen the research on the basic and applied technology of edible dock in animal husbandry and soil improvement.

The research aims to study the status of molybdenum (Mo) in soils of China, optimize the application technology of molybdenum fertilizer, improving the absorption and utilization efficiency of Mo by crops, and promoting crop growth and yield. The content of total Mo and available Mo in soils of China was clarified through collecting data, the supply of soil Mo was evaluated, and the effects of different molybdenum fertilizer application rates and methods on crop growth and yield were studied. The range of total Mo content in soils of China was 0.1-6 mg/kg, with an average of 1.7 mg/kg, slightly lower than the world average; the content of available Mo in soil was affected by parent material of soil formation, soil pH value, etc., and the available Mo content was low in northern loess parent material soil and acidic soil in the south; the application of molybdenum fertilizer could significantly improve the yield and quality of crops such as soybeans, and the effects of seed treatment and foliar spraying were significant. The content of Mo in soils of China is insufficient, especially in northern loess parent material soil and acidic soil in the south, and molybdenum fertilizer should be applied to improve the availability of soil Mo; the rational application of molybdenum fertilizer is of great importance for improving crop yield and quality, especially in areas with low Mo content; the application methods of molybdenum fertilizer are diverse, and seed treatment and foliar spraying are effective application methods that can improve the absorption and utilization of Mo by crops.

Neolissocheilus hexagonolepis, a valuable cold-water fish species, embodies significant economic and scientific potential. The paper provides reference for further research, protection, development and utilization of Neolissocheilus hexagonolepis. Drawing upon a thorough examination of existing literature and the most recent global advancements, this comprehensive review meticulously outlines the findings about its distribution patterns, biological attributes, artificial propagation techniques, and nutritional merits. The investigation highlights key research avenues such as reproductive biology, molecular mechanisms, dietary preferences, resource assessment, nutritional significance, integrated farming practices, and the correlation between weight and length. However, amidst the escalating challenges posed by global climate change, including droughts, floods, extreme heatwaves, low temperature stress, intensified extreme weather events, and human intervention, the population of Neolissocheilus hexagonolepis is experiencing a steady decline, prompting its classification as an endangered species by the IUCN. Consequently, it is imperative to expedite breakthroughs in artificial breeding technologies for this species, establish robust systems and standards for artificial seed preservation, and harness modern biotechnology to unravel and preserve its unique genetic resources, thereby ensuring its preservation and sustainable development.

This article provides a comprehensive overview of the current application status and research progress of Gymnema sylvestre, with a particular emphasis on its pivotal role in agricultural practices and plant protection strategies. From the analysis of chemical constituents and the exploration of pharmacological mechanisms, it reviews the research endeavors on the resources of the Gymnema genus. The bioactive compounds within G. sylvestre, including alkaloids, flavonoids, polysaccharides are crucial to its pharmacological effects in areas such as hypoglycemic, antioxidant, and anti-inflammatory activities. Concurrently, the article delves into the potential of G. sylvestre as a natural biopesticide or growth regulator, emphasizing its capacity to significantly reduce the reliance on chemical pesticides. The total triterpenoid saponins, flavonoid glycosides, anthocyanins, and other components extracted from G. sylvestre exhibit pesticidal effects against agricultural pests, attract beneficial predators, and inhibit the expression of genes associated with pest resistance, thereby offering novel insights into green plant protection. As a plant resource with vast potential for application, the research of G. sylvestre not only enriches the fields of plant chemistry and pharmacology but also presents new avenues for addressing practical issues in modern agriculture. Its significance lies in promoting the green development of agriculture and enhancing human health levels, marking a milestone in advancing sustainable and holistic agricultural practices.

Cold stress directly affects plant growth and development, and under extreme conditions, it may result in infertility or even lead to plant mortality. To investigate the molecular mechanisms by which plants respond to cold stress, this review synthesizes the diverse effects of cold stress on plants. It discusses the impact of cold stress on plant plasma membranes, the ICE-CBF-COR signaling pathway, plant hormones, and cellular metabolism. Additionally, recent advancements in understanding the mechanisms underlying plant cold tolerance are examined and discussed. This review aims to provide a foundation for the practical application of these findings in the genetic improvement of crops. Based on the above, the paper suggests that a multidisciplinary approach, incorporating genetic engineering, genetics, biochemistry, molecular biology, and bioinformatics should be employed to further explore the molecular mechanisms behind plant cold tolerance. Furthermore, potential future research directions in this field are proposed.

The microorganism has the strong material decomposition and the transformation ability, may produce the rich secondary metabolite, the fermentation Chinese traditional medicine is an important processing technology of Chinese traditional medicine processing. Probiotics can regulate the balance of animal gastrointestinal flora, produce many kinds of active enzymes and other metabolites, promote the development of animal immune system organs, participate in many physiological functions of the body, such as substances synthesis of sugar, nitrogen, lipids, inorganic salt metabolism and vitamin, and enhance the body’s disease resistance. The technology of probiotics fermenting traditional Chinese medicine has many advantages, such as improving drug effect, degrading macromolecular active substance, reducing toxic and side effects, promoting animal growth and improving immunity, etc., which is an ideal feed additive after end prohibition. In this paper, the kinds of probiotics, technological types, mechanism and efficacy of probiotics fermenting traditional Chinese medicine and its application in animal breeding are described respectively; it provides a theoretical reference for the development of new anti-feed additives.

Excessive or irrational application of nitrogen (N) fertilizer not only increases N losses, decreases N use efficiency (NUE) and affect yield improvement in cropland, but also causes some environmental problems. Nitrification inhibitors (NIs) can reduce N losses and improve NUE through inhibiting nitrification process. Although numerous studies about NIs focused on the reduction of nitrous oxide (N₂O) emissions and promotion of yield in cropland in China, systematic analysis was still poor. In this paper, meta-analysis was used to study the effects of N fertilizer combined with NIs on the reduction of N₂O emissions, major crop yield and NUE in cropland in China, so as to clarify the influences of soil pH, N application amounts and crop types on effects of NIs. The results showed that N fertilizer combined with NIs reduced the N₂O emissions effect from cropland by 38.66% significantly (P<0.05), increase the effect of major crop yield by 7.31% (P>0.05), and enhance the effect of crop N uptake and NUE by 10.97% (P>0.05) and 25.64% (P>0.05), respectively. Among the different types of NIs, DMPP reduced the effect of N₂O emissions to maximum extent (57.30%), followed by CP and DCD, which reduced the effect of N₂O emissions by 35.07% and 32.28%. CP enhanced the effect of yield to maximum extent (12.76%), followed by DCD (5.65%) and DMPP (4.25%). The reduction size of NIs on N₂O emissions in high N input cropland (≥300 kg/hm²) was greater than that in medium and low N input cropland (<300 kg/hm²). The increasing effect of NIs on yield of main crops in medium N input cropland (200-300 kg/hm²) was greater than that in high and low N input cropland. The effects of NIs on reducing N₂O emissions and increasing crop yield in neutral soil were greater than those in acidic and alkaline soil. Principal component analysis (PCA) showed that soil pH was the main factor affecting crop yield and N₂O emission under the condition of N fertilizer applied with NIs. To sum up, N fertilizer combined with NIs could reduce N₂O emissions significantly, increase crop yield, improve N uptake and NUE to a certain extent, which was an effective path to decrease N losses and promote N utilization.

In order to understand how the herbivores-induced plant volatiles attract natural enemies for pest defense, the recent research status, influencing factors and progress of research on herbivores-induced plant volatiles around the world were reviewed. The release and action effect of plant volatiles are influenced by multiple factors, and their action pathways are closely related to the chemoreceptor genes of insects. In recent years, studies on the action mechanisms of plant volatiles attracting natural enemies have been carried out extensively at home and abroad, and most of them focus on the identification of volatile compounds, behavioral selection experiments and the identification and functional analysis of chemoreceptor genes associated with natural enemies. By analyzing and summarizing the studies, the future development directions on the biological control of pests by natural enemies lured by plant volatiles and related genes were discussed, aiming to provide theoretical support for future development of green plant protection and sustainable agriculture.

This study presents an analysis of the research of purple corn anthocyanins based on 217 relevant literature from the Web of Science core collection from 1997 to 2023. The data was visualized using CiteSpace software to explore the annual publication trends, keywords, countries/regions, and institutions. The findings indicate a significant increase in the number of publications in the field of purple corn anthocyanins. The main research topics in this field include the functional activities, chemical composition, stability, and extraction methods of purple corn anthocyanins. The current research frontiers in this field encompass four aspects: the effect of purple corn on reducing or preventing diseases, the new extraction technology, the stability of anthocyanins and the application of animal husbandry. The top five research institutions in this field are from the United States, China and Thailand. The United States focuses on traditional research areas such as functional activities, stability, and extraction process, while China and Thailand concentrate on the emerging field of applied research in animal husbandry. The application of purple corn anthocyanins in livestock farming may become a future research focus.

Plants and soil microorganisms are closely related, and both play a central role in the carbon cycle mediated by soil microorganisms, they jointly maintain the global carbon balance and the function of ecosystems. Firstly, an overview of the carbon cycle mediated by soil microorganisms was provided. Secondly, the impact of plants on the carbon cycle mediated by soil microorganisms was analyzed, which was specifically reflected in plant photosynthesis, plant respiration, decomposition of plant litter, secretion of plant aboveground secretions, and the rhizosphere effect produced by plants. Among them, the impact of the rhizosphere effect produced by plants on the carbon cycle mediated by soil microorganisms was also reflected in the secretion of plant root exudates and the symbiosis between plants and soil microorganisms. Finally, the impact pathways of plants on the carbon cycle mediated by soil microorganisms were summarized. By reviewing the impact of plants on the carbon cycle mediated by soil microorganisms, it could deepen the understanding of soil carbon cycling and provide a scientific foundation for future environmental protection.

The cultivation area of Astragalus membranaceus was expanding year by year. However, due to the continuous cropping obstacles, the problems of pests and diseases are becoming more and more serious year by year. In order to provide a basis for the identification and integrated prevention and control of diseases in the planting process of A. membranaceus, this paper reviews the occurrence patterns, pathogen species and prevention and control measures of the main diseases of A. membranaceus. The common diseases of A. membranaceus mainly include root rot, verticillium wilt, powdery mildew, downy mildew, damping-off and leaf spot, etc. Among these diseases, root rot and powdery mildew are particularly harmful to A. membranaceus. For these diseases, traditional prevention and control measures mainly rely on chemical agents, such as carbendazim, thiophanate-methyl and fludioxonil, etc. However, long-term dependence on chemical agents may not only have a negative impact on A. membranaceus itself, but also cause environmental pollution. The use of microorganisms such as Bacillus, Trichoderma and Streptomyces can effectively control the occurrence of diseases, while reducing the impact on the environment. In addition to the above methods, the cultivation of A. membranaceus varieties with strong resistance can fundamentally reduce the occurrence of diseases. Moreover, optimizing cultivation methods and strengthening field management, such as rational crop rotation, improving soil structure, controlling irrigation and fertilization, are also key measures to prevent diseases. In summary, the prevention and control of A. membranaceus diseases should adopt a comprehensive management strategy, including biological control, breeding of disease-resistant varieties, optimization of cultivation techniques and strengthening of field management, so as to achieve efficient and environmentally friendly disease control.

In order to mitigate the adverse effects of drought on the development of summer maize seedlings caused by climate change, using ‘Zhengdan 958’ as experimental material, the effects of different treatment combinations on soil moisture and growth of maize seedling were studied. The results showed that the soil moisture content increased with the depth of the soil layer, and the pattern was moist soil, wheat straw returning to the field>normal moisture content, wheat straw returning to the field>moist soil, wheat straw not returning to the field>dry soil, wheat straw returning to the field>normal moisture content, wheat straw not returning to the field>dry soil, wheat straw not returning to the field. SPAD value, photosynthetic rate and yield showed the same trend, normal moisture content, wheat straw returning to the field>moist soil, wheat straw returning to the field>moist soil, wheat straw not returning to the field>normal moisture content, wheat straw not returning to the field>dry soil, wheat straw returning to the field>dry soil, wheat straw not returning to the field. Therefore, compared with the removal of stubble, plain stubble can better improve the soil water environment, increase the emergence rate and photosynthetic rate of maize, and increase the yield, which can be widely applied in production.

Peppermint is an herb with a long history in China, and China is also one of the largest peppermint growing countries in the world. Peppermint contains volatile oil and flavonoids, of which menthol is the key component, which has antibacterial and antioxidant effects. Menthol has attracted attention due to its cool aroma and pharmacological effects. This paper reviewed the research progress of menthol detection, extraction and application, including its physical and chemical properties and pharmacological effects. The detection techniques such as gas chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry, as well as the extraction techniques such as CO₂ supercritical extraction, organic solvent extraction and microwave-assisted extraction were introduced. The article also summarized the application of menthol in medicine, food, cosmetics and other fields, and prospects its future development. In depth study of menthol is of great significance to the implementation of national agricultural, rural and farmer policies and rural revitalization strategies.

L-theanine is a special free amino acid in tea leaves and one of the main flavor substances in tea. Due to its health benefits such as relaxation, fatigue relief, anti-tumor effects, and blood pressure reduction, it has received widespread attention in the food and healthcare industries. In order to study the synthesis pathway and methods of L-theanine, this paper summarized the current main pathways for L-theanine synthesis, which were divided into plant-derived synthesis and microbial-derived synthesis according to their sources; and bioenzymatic catalysis and chemical synthesis according to their synthesis methods. The advantages and disadvantages of each synthesis method were analyzed. Meanwhile, the applications of L-theanine in food and medical fields were summarized, and future research directions for efficient theanine synthesis were proposed, aiming to provide a reference for the production and application of L-theanine.

The Huang-Huai-Hai region is a significant production area for winter wheat and summer maize in China. Currently, the limiting factors include poor maize seeding quality due to no-tillage planting after wheat harvesting, difficulty in irrigation during the sowing to emergence period, untimely irrigation and fertilization, and poor farming techniques. Further enhancement of the use efficiency of radiation, temperature, water and fertilizer resources is the crucial way to achieve high grain yield and sustainable, green agricultural development in this region. To addresses these challenges, since 2018, we have innovated a comprehensive solution integrating several new technologies including the “four to one narrow-wide strip planting” for winter wheat, satellite-guided precision planting, annual shallow subsoil drip irrigation for synchronizing water-fertilizer-pesticide management. The corresponding modern agricultural machinery and information technology have been also matched. The integrated technique is called “Green water-fertilizer-pesticide synchronizing technology characterized of ‘four to one narrow-wide strip planting’ plus shallow subsoil drip fertigation for winter wheat-summer maize cropping system”. The field demonstration experiments conducted between 2010 and 2013 indicated that this novel comprehensive technology effectively addressed the aforementioned challenges and achieved both high yield and efficient resource utilization. Compared with traditional farmer practice, the new technology increased grain yield by 9%-17% in winter wheat and by 12%-14% in summer maize. The new technology also saved water input by 450-750 m³/hm², fertilizer input by 20%, and labor cost by 2250-3000 yuan/hm². This comprehensive technology provides a novel feasible solution for the green and high-yielding production of winter wheat and summer maize in the Huang-Huai-Hai region.

Cold plasma technology has gained significant traction in agriculture due to its advantages, including low operational temperature, green, residue-free, high efficiency, and remarkable effectiveness in seed treatment, as well as in activating the internal physiological and biochemical processes of plants to improve resistance and yield. This paper reviews the progress of cold plasma technology in seed disinfection, breaking seed dormancy to promote germination, enhancing plant resistance, and increasing yield, etc. Furthermore, this paper provides a comprehensive analysis of the primary active ingredients of this technology and elucidates their mechanisms of action at the physicochemical, physiological and biochemical levels. In view of the differences in physical, chemical and physiological effects of different treatment objects after discharge by different types of cold plasma equipment, the future research direction is proposed: optimizing and establishing cold plasma treatment system, and clarifying the long-term effect of cold plasma treatment on the phenotypic genetic changes of plant growth cycle.

In order to further understand the application of Integrated Multi-Trophic Aquaculture (IMTA) in aquaculture industry, the paper sorted out the development history of IMTA, and outlined the main aquaculture species of IMTA, including fishes, crustaceans, bivalves, echinoderms and algae, et al. It summarized land-based and marine IMTA, and pointed out that freshwater and brackish water IMTA were part of the land-based aquaculture models, while seawater and offshore IMTA belonged to marine IMTA. The article also outlined the advantages of IMTA in terms of aquaculture environment, energy utilization, economic benefits, and reduction of waste discharge. At present, IMTA still has problems in the aspects of technical implementation, ecological impacts and sustainable development. Future research should focus on the interactions among different species components, system optimization, and disease control to promote the sustainable development of aquaculture.

Straw biochar plays an important role in environmental governance and resource recycling. In order to grasp the development process of straw biochar and clarify the focus and preface direction of this research field, this article uses CiteSpace visualization software to conduct bibliometric and knowledge graph analysis on the data retrieved from the Web of Science (WOS) core collection database. The results show that the annual publication volume is on the rise, with China ranking first with a total of 3141 articles; among the global research institutions, Chinese Academy of Sciences, Ministry of Agriculture and Rural Affairs and Institute of Soil Science are representative institutions for straw biochar research; the research results in this field are mainly published in high impact factor journals such as Science of the Total Environment and Bioresource Technology, Wang Hailong from China is the scholar with the highest number of publications; the research hotspots and directions mainly include the raw materials and preparation conditions of straw biochar, the application and mechanisms of straw biochar in carbon sequestration and emission reduction, soil improvement, and pollution remediation. Overall, straw biochar is a hot topic in the resource utilization of agricultural waste. In the future, the modification and preparation of straw biochar, as well as the mechanism and long-term effects of mitigating climate change and repairing environmental pollution, will be the research hotspot and trend.

In order to select the optimal red and blue light ratio for the growth of hydroponic leeks in an artificial light plant factory hydroponic system, Chinese chives ‘791’ were utilized as test materials, and five different red and blue light ratio treatments (R:B) were established, namely T₂ (1:1), T₃ (3:1), T₄ (5:1), T₅ (7:1), and T₆ (9:1). A white light treatment (T₁) was employed as a control. The impact of varying red and blue light ratios on the growth indicators, physiological markers, and flavor quality of hydroponic Chinese chives was studied. The results showed that compared with the control, treatments from T₂ to T₄ had no significant effect on the growth indicators of Chinese chives, but significantly increased yield. The yield increases for T₄, T₅, and T₆ treatments reached 38.10%, 41.26%, and 57.19% respectively. Although the T₆ treatment significantly improved the chlorophyll fluorescence parameters (Fv/Fm, Fv/Fo), the total phenols, flavonoids, and vitamin content decreased significantly by 32.43%, 31.18%, and 55.68% respectively. Flavor analysis showed that an increase in the proportion of red light promoted the production of specific flavor substances such as W5S, W2S, and CSO, while the content of W1S decreased correspondingly. In the T₆ treatment, the increase in high red light proportion promoted growth, yield, and accumulation of specific flavor substances, while reduced nutritional value and photosynthetic pigment content. Comprehensive analysis of yield, nutritional quality, and flavor factors suggested that in the cultivation of chives, it was recommended to use a red-blue light ratio range of T₄ (R:B=5:1) to T₅ (R:B=7:1), which can yield higher outputs and superior flavor quality in Chinese chives cultivation.

In order to provide theoretical basis, identification method and selection index for the identification and screening of low nitrogen tolerant maize varieties, the research reports on the influence of low nitrogen stress on maize growth and development, the material basis of low nitrogen tolerance and the selection of low nitrogen tolerant varieties in maize at home and abroad in the past 20 years were summarized. It was found that low nitrogen stress seriously affected the growth and development of maize roots and plants, and grain yield. However, different genotypes of maize showed great differences in morphological characteristics, physiological and biochemical characteristics, nitrogen utilization efficiency, and grain yield. Under low nitrogen stress, the low nitrogen tolerant genotype showed that the root system grew well, root-shoot ratio was larger, the root system vigor was stronger. Meanwhile, the changes in plant height and stem thickness were not obvious, while the ear leaf area was larger. It was higher for the content of soluble protein, soluble sugar, and amino acids in its root secretions as well as the contents of chlorophyll and nitrogen in ear leaf. Nitrate reductase and glutamine synthetase activity were stronger in the cells, and the ear was larger, grains per ear were much and 100-grain weight was higher, these genotypes could obtain the high grain yield. It was considered that under low nitrogen stress, the biomass of roots and plants was high, the root-shoot ratio and ear leaf area were large, the content of soluble protein and soluble sugar in root exudates, the content of chlorophyll and nitrogen in ear leaves were high, the activity of nitrate reductase and glutamine synthetase in cells was strong, the number of grains per ear was large, and the weight of 100 grains and the weight of grains per ear were high. The above information could be used as important indicators for selecting low nitrogen tolerant maize genotypes.

This study aims to improve the efficiency of cucumber green mottle mosaic virus (CGMMV)-mediated virus-induced gene silencing (VIGS) in cucurbit crops. Using germplasms from cucumber, melon and watermelon, effectiveness in gene silencing was tested by using various inoculation methods with CGMMV-VIGS vector, cultivation temperatures, and relative humidity in incubator. The results demonstrated that vacuum infiltration and seed imbibition were the most effective inoculation methods, achieving the highest frequency of gene silencing (FGS) in cucurbit crops. Cucumber, melon, and watermelon showed high effectiveness of PDS silencing EGSL (100%) at the temperatures of 22℃ and 25℃ after one month of growth, significantly higher than the EGSL at 30℃. After three months, the EGSL for melon and watermelon were 89.7% and 95%, respectively at 22℃ and 85.6% and 86.1% at 25℃, both significantly higher than that at 30℃. With 30% and 50% relative humidity in incubator, the EGSL for cucumber were 70% and 72%, respectively, which were significantly higher than the EGSL at 80% humidity. For watermelon, the EGSL at 30% relative humidity was 69%, significantly higher than the EGSL at 50% humidity (38%) and 80% humidity (33%). Overall, by optimizing the inoculation methods and environmental parameters in the CGMMV-VIGS, we have improved the gene silencing rate and effectiveness in cucurbit crops. This method will facilitate rapid acquisition of VIGS germplasm resources for research about crop quality and abiotic stress resistance.

The transcription profile of Micropterus salmoides against LMBV infection was analyzed to reveal the immune response mechanism of Micropterus salmoides in response to LMBV infection. The head kidney tissues of largemouth bass infected with LMBV 72h were analyzed by transcriptome sequencing using Illumina NovaSeq 6000 sequencing platform. A total of 5953 differentially expressed genes (DEGs) were obtained, of which 1704 were up-regulated and 4249 were down-regulated. Through GO function annotation, DEGs were mainly related to single-organism process, metabolic process, membrane, cell, binding, catalytic activity, etc. The KEGG enrichment analysis showed that DEGs were mainly enriched in metabolic pathways such as glycolysis, primary bile acid biosynthesis. There were 4 immune response-related signaling pathways enriched including cytokine-cytokine receptor interaction, ECM-receptor interaction, phagosome and intestinal immune network for IgA production. The immune-related increased DEGs were cxcr4, il10, mrc, ncf4, itgb2, il27, ccl25, etc. while the decreased degs were cxcl12, tgfb3, il20ra, col4a5, itgb1, etc. PPI analysis showed that itgb1, itga8 and itgb6 were the hub genes of immune-related DEGs. This study analyzed the transcription profile of largemouth bass infected with LMBV to provide a theoretical basis for the molecular mechanism and disease prevention of largemouth bass immunity against LMBV.