In order to effectively control Huanglongbing (HLB) and reduce the loss of HLB to the global citrus industry, this study reviewed the latest research on the control of HLB in recent years. The results showed that there were no effective agents and disease-resistant varieties. At present, the main methods of controlling HLB include traditional ‘three fast methods’, namely planting disease-free seedlings to control HLB from the sources, preventing and controlling Diaphorina citr to block its transmission path, and digging infected trees to eliminate the infection source; expanded physical control technologies include seedling virus-free, light, steam heat treatment, etc.; multi-directional chemical drug control technologies include antibiotics, nanomaterials, immune inducers and antimicrobial peptides; emerging biological control technologies include various probiotics, microbial preparations, etc. Finally, the application prospect of soil amendment and biological control agent combined treatment of HLB and citrus transgenic disease resistance technology based on genetic improvement was prospected, to provide theoretical basis and practical reference for effective control of HLB.

The research aims to study the status of molybdenum (Mo) in soils of China, optimize the application technology of molybdenum fertilizer, improving the absorption and utilization efficiency of Mo by crops, and promoting crop growth and yield. The content of total Mo and available Mo in soils of China was clarified through collecting data, the supply of soil Mo was evaluated, and the effects of different molybdenum fertilizer application rates and methods on crop growth and yield were studied. The range of total Mo content in soils of China was 0.1-6 mg/kg, with an average of 1.7 mg/kg, slightly lower than the world average; the content of available Mo in soil was affected by parent material of soil formation, soil pH value, etc., and the available Mo content was low in northern loess parent material soil and acidic soil in the south; the application of molybdenum fertilizer could significantly improve the yield and quality of crops such as soybeans, and the effects of seed treatment and foliar spraying were significant. The content of Mo in soils of China is insufficient, especially in northern loess parent material soil and acidic soil in the south, and molybdenum fertilizer should be applied to improve the availability of soil Mo; the rational application of molybdenum fertilizer is of great importance for improving crop yield and quality, especially in areas with low Mo content; the application methods of molybdenum fertilizer are diverse, and seed treatment and foliar spraying are effective application methods that can improve the absorption and utilization of Mo by crops.

Peppermint is an herb with a long history in China, and China is also one of the largest peppermint growing countries in the world. Peppermint contains volatile oil and flavonoids, of which menthol is the key component, which has antibacterial and antioxidant effects. Menthol has attracted attention due to its cool aroma and pharmacological effects. This paper reviewed the research progress of menthol detection, extraction and application, including its physical and chemical properties and pharmacological effects. The detection techniques such as gas chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry, as well as the extraction techniques such as CO₂ supercritical extraction, organic solvent extraction and microwave-assisted extraction were introduced. The article also summarized the application of menthol in medicine, food, cosmetics and other fields, and prospects its future development. In depth study of menthol is of great significance to the implementation of national agricultural, rural and farmer policies and rural revitalization strategies.

Cold stress directly affects plant growth and development, and under extreme conditions, it may result in infertility or even lead to plant mortality. To investigate the molecular mechanisms by which plants respond to cold stress, this review synthesizes the diverse effects of cold stress on plants. It discusses the impact of cold stress on plant plasma membranes, the ICE-CBF-COR signaling pathway, plant hormones, and cellular metabolism. Additionally, recent advancements in understanding the mechanisms underlying plant cold tolerance are examined and discussed. This review aims to provide a foundation for the practical application of these findings in the genetic improvement of crops. Based on the above, the paper suggests that a multidisciplinary approach, incorporating genetic engineering, genetics, biochemistry, molecular biology, and bioinformatics should be employed to further explore the molecular mechanisms behind plant cold tolerance. Furthermore, potential future research directions in this field are proposed.

Fusarium oxysporum (Fo) is a soil-borne fungal disease that causes crop wilting, which is a serious constraint to the sustainable development of agriculture. Effectors are key factors in pathogen-plant interactions, and their mechanism of action can be analyzed to provide effective strategies for controlling plant diseases. When Fo infects crops, it secretes a protein in the xylem of vascular bundles, which is called Secreted In Xylem effector protein (SIX). In order to study the role of SIX effectors between Fo and plants, the structural characteristics of SIX on the Fo genome were analyzed. The functional attributes of SIX in identifying different formae speciales and physiological races of Fo, influencing the pathogenicity of Fo, and affecting the immune response of plants were summarized. It was pointed out that SIX had toxic effects on plants and could interact with plants in an incompatible manner to influence the invasion of pathogens. The results of this study reveal the effects of SIX on plants in different aspects and provide theoretical support for the study of the molecular mechanism of plant disease resistance and the selection and breeding of disease-resistant varieties.

In this paper, the research status of edible dock was briefly described from the aspects of nutritional value, safety, extraction technology of protein and active substances, food and health products, feeding, soil improvement and cultivation technology, etc. It was analyzed that the values of nutrition, health care, food use, feeding use and soil improvement of edible dock were high, but people’s recognition of leaf-eating grass was low, the related basic and applied technology research was still in the initial stage, and the development of the industry was slow. Three suggestions were put forward to promote the research and industrial development of edible dock, to improve people’s recognition of edible dock through propaganda and production of high-value products of edible dock; accelerate the research on the introduction and cultivation of edible dock, and promote intensive and large-scale planting; strengthen the research on the basic and applied technology of edible dock in animal husbandry and soil improvement.

Abundant crop straw production in our country makes anaerobic dry fermentation for biogas production an important way to utilize crop straw resources. However, the dense structure of lignocellulose in straw limits the hydrolysis and acidification of straw, making it difficult for anaerobic bacteria to degrade straw and produce biogas. Currently, pretreatment of straw is considered to be an effective way to enhance the rate of anaerobic digestion and improve biogas yield. The text elaborates on the mechanism of anaerobic dry fermentation, and reviews the common pretreatment methods that can increase the efficiency of biogas production in straw anaerobic dry fermentation, including physical, chemical, biological, and combined pretreatment methods, and provides a perspective on the technology of straw pretreatment.

In order to explore the stress of water environment changes on cultured fish in the development of intensive aquaculture, the effects of environmental factors such as temperature, pH, dissolved oxygen, ammonia nitrogen and heavy metals on fish body were studied. From the five aspects of growth performance, blood biochemistry, digestive enzyme activity, antioxidant capacity and immunity, the related mechanism of environmental stress factors affecting fish health was reviewed, and the importance of environmental stress factor research was revealed. It was suggested that comprehensive methods such as field investigation, long-term monitoring and calculation models should be more widely used in the future to comprehensively explore and evaluate the coping strategies and health status of fish in the actual environment, and to explore the molecular mechanism of environmental stress factors on fish health. Future experiments need to study the whole process of fish growth in different periods, which is helpful to predict the impact of future environmental changes on fish community structure and ecosystem function. By studying the adverse effects of environmental stress factors on fish health, the occurrence of stress can be avoided or weakened, which provides a reference for ensuring efficient and healthy aquaculture of fish.

This article provides a comprehensive overview of the current application status and research progress of Gymnema sylvestre, with a particular emphasis on its pivotal role in agricultural practices and plant protection strategies. From the analysis of chemical constituents and the exploration of pharmacological mechanisms, it reviews the research endeavors on the resources of the Gymnema genus. The bioactive compounds within G. sylvestre, including alkaloids, flavonoids, polysaccharides are crucial to its pharmacological effects in areas such as hypoglycemic, antioxidant, and anti-inflammatory activities. Concurrently, the article delves into the potential of G. sylvestre as a natural biopesticide or growth regulator, emphasizing its capacity to significantly reduce the reliance on chemical pesticides. The total triterpenoid saponins, flavonoid glycosides, anthocyanins, and other components extracted from G. sylvestre exhibit pesticidal effects against agricultural pests, attract beneficial predators, and inhibit the expression of genes associated with pest resistance, thereby offering novel insights into green plant protection. As a plant resource with vast potential for application, the research of G. sylvestre not only enriches the fields of plant chemistry and pharmacology but also presents new avenues for addressing practical issues in modern agriculture. Its significance lies in promoting the green development of agriculture and enhancing human health levels, marking a milestone in advancing sustainable and holistic agricultural practices.

Peppermint is a food-medicine crop with secondary metabolites as core functional components. It has a wide range of biological activities. The bioactivities of peppermint are mainly related to its abundant active constituents, such as menthol, menthone, montaflorin, hesperidin, caffeic acid and other constituents, which have anti-inflammatory, antioxidant, antibacterial and antitumour effects. This paper reviews the distribution of resources and major origins of peppermint, the analytical studies of the major functional components of peppermint, the progress of nutritional and pharmacological studies of the major functional components, as well as the prediction and quality control of the core quality components of peppermint. Finally, the application fields and prospects of peppermint in agricultural product processing are discussed to provide direction and basis for the application of peppermint processing.

L-theanine is a special free amino acid in tea leaves and one of the main flavor substances in tea. Due to its health benefits such as relaxation, fatigue relief, anti-tumor effects, and blood pressure reduction, it has received widespread attention in the food and healthcare industries. In order to study the synthesis pathway and methods of L-theanine, this paper summarized the current main pathways for L-theanine synthesis, which were divided into plant-derived synthesis and microbial-derived synthesis according to their sources; and bioenzymatic catalysis and chemical synthesis according to their synthesis methods. The advantages and disadvantages of each synthesis method were analyzed. Meanwhile, the applications of L-theanine in food and medical fields were summarized, and future research directions for efficient theanine synthesis were proposed, aiming to provide a reference for the production and application of L-theanine.

The microbial composition and function in the rhizosphere is the research focus of microbial ecology. Analysis of the diversity of soil microbial communities in the root system of paddy rice and the effects of environmental factors on soil microorganisms can provide reference value for the sustainability of soils in the growing areas of paddy rice and the conservation of land resources. In this study, we used 16S rRNA high-throughput sequencing technology to determine the bacterial composition of soil microorganisms in the rhizosphere of paddy rice, and analyze the composition and diversity of microbial communities sampled from different regions, and explore the relationship between soil microbial diversity and soil physicochemical properties. The results showed that a total of 33862 OTUs, belonging to 38 phylums, 88 orders, 214 orders, 514 families and 2268 genera, were obtained from the paddy rice rhizosphere soil samples collected from the four regions by sequencing. At the phylum level, the dominant phyla of paddy rice rhizosphere soil bacteria collected from the four regions were Proteobacteria, Bacteroidetes and Firmicutes. At the genus level, Limisphaera was the dominant taxon of paddy rice rhizosphere soil bacteria. As shown by the Alpha diversity index, the species diversity and richness of paddy rice inter-root soil bacteria collected from the four regions were generally as followed: 68^th Regiment of the Corps (D)> Dairy Farm of Yili Prefecture (A)> Chabchal Town (B)> Sundzach Niuzhu Township (C); the Beta diversity index indicated that the paddy rice rhizosphere soil collected from the four regions differed accordingly in their bacterial community compositions, with greater differences existed between group A and other groups. The correlation analysis between soil bacteria and environmental factors concluded that the main influencing factors on the composition of microbial bacterial communities in paddy rice rhizosphere soils might be nitrogen, total potassium, and organic carbon. This study clarified the rhizosphere soil conditions and rhizosphere soil bacterial community characteristics of paddy rice distributed in the Ili River Valley, and provided theoretical support for the study of soil ecosystems in the Ili River Valley.

The cultivation area of Astragalus membranaceus was expanding year by year. However, due to the continuous cropping obstacles, the problems of pests and diseases are becoming more and more serious year by year. In order to provide a basis for the identification and integrated prevention and control of diseases in the planting process of A. membranaceus, this paper reviews the occurrence patterns, pathogen species and prevention and control measures of the main diseases of A. membranaceus. The common diseases of A. membranaceus mainly include root rot, verticillium wilt, powdery mildew, downy mildew, damping-off and leaf spot, etc. Among these diseases, root rot and powdery mildew are particularly harmful to A. membranaceus. For these diseases, traditional prevention and control measures mainly rely on chemical agents, such as carbendazim, thiophanate-methyl and fludioxonil, etc. However, long-term dependence on chemical agents may not only have a negative impact on A. membranaceus itself, but also cause environmental pollution. The use of microorganisms such as Bacillus, Trichoderma and Streptomyces can effectively control the occurrence of diseases, while reducing the impact on the environment. In addition to the above methods, the cultivation of A. membranaceus varieties with strong resistance can fundamentally reduce the occurrence of diseases. Moreover, optimizing cultivation methods and strengthening field management, such as rational crop rotation, improving soil structure, controlling irrigation and fertilization, are also key measures to prevent diseases. In summary, the prevention and control of A. membranaceus diseases should adopt a comprehensive management strategy, including biological control, breeding of disease-resistant varieties, optimization of cultivation techniques and strengthening of field management, so as to achieve efficient and environmentally friendly disease control.

Cold plasma technology has gained significant traction in agriculture due to its advantages, including low operational temperature, green, residue-free, high efficiency, and remarkable effectiveness in seed treatment, as well as in activating the internal physiological and biochemical processes of plants to improve resistance and yield. This paper reviews the progress of cold plasma technology in seed disinfection, breaking seed dormancy to promote germination, enhancing plant resistance, and increasing yield, etc. Furthermore, this paper provides a comprehensive analysis of the primary active ingredients of this technology and elucidates their mechanisms of action at the physicochemical, physiological and biochemical levels. In view of the differences in physical, chemical and physiological effects of different treatment objects after discharge by different types of cold plasma equipment, the future research direction is proposed: optimizing and establishing cold plasma treatment system, and clarifying the long-term effect of cold plasma treatment on the phenotypic genetic changes of plant growth cycle.

Straw biochar plays an important role in environmental governance and resource recycling. In order to grasp the development process of straw biochar and clarify the focus and preface direction of this research field, this article uses CiteSpace visualization software to conduct bibliometric and knowledge graph analysis on the data retrieved from the Web of Science (WOS) core collection database. The results show that the annual publication volume is on the rise, with China ranking first with a total of 3141 articles; among the global research institutions, Chinese Academy of Sciences, Ministry of Agriculture and Rural Affairs and Institute of Soil Science are representative institutions for straw biochar research; the research results in this field are mainly published in high impact factor journals such as Science of the Total Environment and Bioresource Technology, Wang Hailong from China is the scholar with the highest number of publications; the research hotspots and directions mainly include the raw materials and preparation conditions of straw biochar, the application and mechanisms of straw biochar in carbon sequestration and emission reduction, soil improvement, and pollution remediation. Overall, straw biochar is a hot topic in the resource utilization of agricultural waste. In the future, the modification and preparation of straw biochar, as well as the mechanism and long-term effects of mitigating climate change and repairing environmental pollution, will be the research hotspot and trend.

In order to further understand the application of Integrated Multi-Trophic Aquaculture (IMTA) in aquaculture industry, the paper sorted out the development history of IMTA, and outlined the main aquaculture species of IMTA, including fishes, crustaceans, bivalves, echinoderms and algae, et al. It summarized land-based and marine IMTA, and pointed out that freshwater and brackish water IMTA were part of the land-based aquaculture models, while seawater and offshore IMTA belonged to marine IMTA. The article also outlined the advantages of IMTA in terms of aquaculture environment, energy utilization, economic benefits, and reduction of waste discharge. At present, IMTA still has problems in the aspects of technical implementation, ecological impacts and sustainable development. Future research should focus on the interactions among different species components, system optimization, and disease control to promote the sustainable development of aquaculture.

This study comprehensively evaluated the preparation techniques of chitosan oligosaccharide (COS) and conducted an in-depth analysis of the effects of COS and microbial metabolites on plant growth, crop quality enhancement, and pest management, aiming to enhance agricultural production efficiency and promote environmental sustainability. The results revealed the importance of COS and microbial metabolites in improving agricultural yield and maintaining environmental stability. In response to the challenges posed to food security by climate and ecosystem changes caused by natural and human activities, we propose that future research should focus on the activities of organic nanoparticles such as COS and microbial metabolites, and emphasize the necessity of advancing emerging fertilizer research to provide scientific basis and technical support for the sustainable development of agricultural production.

The yield and agronomic traits of rice are affected by the cultivation environment and their adaptability, and the varieties with wide adaptability are less affected by the cultivation environment. This study aims to clarify the adaptability of ‘Yunjing 37’, a new japonica rice variety with good eating quality. The stability of yield and agronomic traits of ‘Yunjing 37’ and control varieties were analyzed by AMMI model, which planted in 6 sites in Yunnan Province for two consecutive years. All traits showed highly significant differences between varieties and environment, along with significant interaction effects between varieties and environment. Based on the stability parameters ASV, Di, WAASBi, the grain number, seed setting, 1000-weight, plant height, length and angle of the flag leaf, which were closely related to yield and plant type, ‘Yunjing 37’ showed the best stability among the four varieties. This indicates that different environmental conditions have relatively little influence on the yield and plant type of ‘Yunjing 37’. Therefore, ‘Yunjing 37’ has wide adaptability and is suitable for widespread application in Yunnan japonica rice area.

As an important economic shrimp species, the Litopenaeus vannamei is frequently affected by bacterial diseases, especially Vibriosis (including Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, Vibrio cholerae, etc.) during intensive aquaculture. This represents a significant risk to the long-term sustainability of aquaculture. With the widespread use of antibiotics, resistance genes of pathogenic bacteria are prevalent and widespread in the aquatic environment, posing new challenges to the prevention and treatment of Vibriosis. Therefore, it is extremely important to emphasize the maintenance of aquaculture environment and water quality monitoring. This review describes a series of biological control measures, including the use of biofloc technology, probiotics, antimicrobial peptides, quorum sensing inhibitors, and the use of biological control methods such as Bdellovibrio and like organisms and bacteriophage. The combination of biofloc technology and probiotics can improve water quality and enhance immunity. However, it is greatly affected by the environment, making it difficult to control the stability of bacteria flora. Antimicrobial peptides exhibit a broad spectrum of antibacterial activity, and they are safe and not easily resistant to drugs. However, they are difficult to preserve and have a high cost. Quorum-sensing inhibitors provide precise prevention and treatment with minimal environmental impact, but the mechanism research on them is limited. Bdellovibrio are effective at lysing Gram-negative bacteria but have a poor effect on Gram-positive bacteria and grow slowly. Bacteriophages are highly specific but have a narrow host range. Although these biological control measures are less effective than antibiotics, they are environmentally friendly, less prone to drug resistance, and aim to reduce chemical drug dependency, protect the ecosystem, and improve aquaculture efficiency. In the future, innovative and sustainable biological control technologies should be developed to address the challenges posed by pathogenic bacterial resistance and environmental protection.

This study aims to explore the impact of different extraction methods on the antioxidant activity and stability of polysaccharides from broken Ganoderma lucidum spore powder, and provide a scientific basis for commercial production and stability optimization of products. Polysaccharides were prepared by hot water extraction, ultrasound-assisted extraction, microwave-assisted extraction, and enzymatic hydrolysis-assisted extraction. The polysaccharide yield and antioxidant capacity were determined. Through simulating food processing conditions, the oxidation stability of polysaccharides was systematically evaluated. The polysaccharides extracted from broken Ganoderma lucidum spore powder by enzymatic hydrolysis-assisted method (LPE) had the highest yield and uronic acid content, which were 5.05% and 6.55% respectively. LPE had the strongest antioxidant capacity. The highest scavenging rate of DPPH free radicals was 77.24%, and the highest scavenging rate of hydroxyl radicals was 83.04%. Light exposure had a great influence on the antioxidant stability of LPE. Under acidic conditions, it had better antioxidant activity. The scavenging rate of hydroxyl radicals exceeded 85%. Moreover, LPE showed good heat resistance. In addition, sweeteners and alkaline additives had a significant impact on the antioxidant stability of LPE. For product sterilization, high-pressure sterilization can be preferred. LPE had the strongest antioxidant activity. During the processing and storage of Ganoderma lucidum spore powder polysaccharide products, prolonged light exposure should be avoided, and the dosage of sweeteners and alkaline additives should be controlled to ensure product quality.

In order to obtain a safe, reliable, time-saving and labor-saving pre-treatment technology for pesticide residue detection, this paper focused on the application of nanomaterials in the detection of pesticide residues in fruits and vegetables, discussing various pretreatment methods including solid phase extraction (SPE), matrix-dispersive solid phase extraction (MSPD), QuEChERS, accelerated solvent extraction (ASE), Gel permeation chromatography (GPC), and molecularly imprinted polymer Extraction (MIPs). The research progress in the detection of pesticide residues in fruits and vegetables was also analyzed. The review found that pre-treatment technology was still the key to determine whether the detection of pesticide residues was accurate and efficient. At the same time, the future pre-treatment technology of fruits and vegetables was prospected, aiming at providing theoretical reference for the inspection and detection of pesticide residues in fruits and vegetables and food safety supervision.

Plants and soil microorganisms are closely related, and both play a central role in the carbon cycle mediated by soil microorganisms, they jointly maintain the global carbon balance and the function of ecosystems. Firstly, an overview of the carbon cycle mediated by soil microorganisms was provided. Secondly, the impact of plants on the carbon cycle mediated by soil microorganisms was analyzed, which was specifically reflected in plant photosynthesis, plant respiration, decomposition of plant litter, secretion of plant aboveground secretions, and the rhizosphere effect produced by plants. Among them, the impact of the rhizosphere effect produced by plants on the carbon cycle mediated by soil microorganisms was also reflected in the secretion of plant root exudates and the symbiosis between plants and soil microorganisms. Finally, the impact pathways of plants on the carbon cycle mediated by soil microorganisms were summarized. By reviewing the impact of plants on the carbon cycle mediated by soil microorganisms, it could deepen the understanding of soil carbon cycling and provide a scientific foundation for future environmental protection.

In order to fully understand the impact of strong wind disaster on the maturity stage of Actinidia arguta, the meteorological data of the maturity stage of Actinidia arguta at four stations in Dandong from 1991 to 2020 were used, and the maximum wind speed ≥12.0 m/s was used as the index of strong wind disaster. Considering the frequency, intensity and duration, the risk levels of mild, moderate and severe strong wind disaster were formulated. The variation law of strong wind disaster of Actinidia arguta was analyzed by the number of disasters and the ratio of stations, and the risk probability of strong wind disaster was evaluated based on information diffusion theory. The results showed that the maximum wind speed during the ripening period of Actinidia arguta in Dandong area showed a downward trend in the past 30 years, and the number of days of strong wind process showed a decreasing trend. Among them, the decrease in Zhen'an area was the smallest, and the number of years and days of strong wind was the most, which was more prone to strong wind disaster risk. The time variation characteristics of the occurrence times of different grades of strong wind disasters in the mature period of Actinidia arguta in Dandong area during 30 years were obvious. The occurrence times of three grades of strong wind disasters were mild > moderate > severe, and the occurrence probability was consistent with them, which were 66.5%, 13.4% and 10.0%, respectively. The corresponding historical recurrence periods were more than 2 years, 10 years and 10 years. At the same time, the high risk probability areas corresponding to the three grades of strong wind disasters were Fengcheng (mild), Zhen 'an (moderate) and Donggang (severe). The probability of gale disaster in each region from high to low was Zhen 'an, Fengcheng, Donggang and Kuandian, and their exceeding probabilities were 95.0%, 89.9%, 86.6% and 33.4%, respectively. The gale disaster was in the range of one to three years. The research results obtained the risk probability and return period law of different gale disasters at each site, which provided a reference for scientific response to the gale disaster in the mature period of Actinidia arguta.

This study explores the demand for low temperature accumulation during the germination process of apricot trees and analyzes the impact of warm winter phenomena caused by global warming on apricot yield. In this study, using the high-quality apricot variety ‘Haitanghong’ and its bud variant ‘Zaoyan’ from southern China as experimental materials, through transcriptomic analysis of 550 hours of treatment at 4℃, 3124 differentially expressed genes (DEGs) were identified, and many were associated with plant hormones and protein dephosphorylation. By observing the branch color of the two varieties under low-temperature treatments, we found that the cultivar with the lower chilling requirement was more tolerant to cold. These results suggest that compared to ‘Haitanghong’, ‘Zaoyan’ has a lower low-temperature requirement, and the difference in chilling requirements between the two varieties may be related to plant hormones and post-transcriptional modification. And the reason for these differences of alternative splicing may be associated with the varied chilling requirement in the two cultivars. These results can provide a reference for mitigating the decrease of apricot yield under climate warming.

To address the issues of anthocyanin content and the types of monomeric anthocyanins in mulberries from the Yunnan region, 39 varieties of mulberries were investigated and analyzed for their anthocyanin content. The total anthocyanin content was determined using the pH differential method, the monomeric anthocyanin content was measured using high-performance liquid chromatography (HPLC), and the absorbance of red pigments in the edible parts of the mulberries was detected using spectrophotometry. The results showed that total anthocyanins were detected in 32 out of the 39 mulberry samples, and four types of monomeric anthocyanins were identified: cyanidin, pelargonidin, peonidin, and malvidin. Cyanidin was detected in 36 samples, pelargonidin in 33 samples, peonidin in 1 sample, and malvidin in 4 samples. Red pigments were detected in all 39 samples, with darker-colored mulberry fruits exhibiting higher red pigment content. The varieties ‘YX002’, ‘Hongguo 2’, ‘Jialing 30’, ‘Mengtong 4’, and ‘YX001’ has exhibit high levels of red pigments and anthocyanins, along with a rich diversity of monomeric anthocyanins. Among these, ‘YX002’ can have the highest total anthocyanin content, reaching 59.05 mg/L, and both cyanidin and pelargonidin also be detected in this variety.

Ethylene Responsive Factor (ERF) transcription factors belong to the plant AP2/ERF transcription factor superfamily and are key regulatory factors in plants responses to biotic and abiotic stresses. They bind to the cis-acting element GCC-box through the conserved AP2/ERF domain, thereby regulating the spatiotemporal expression of target genes. This article reviews the structural characteristics, classification system, distribution patterns, and biological functions of plant ERF transcription factors. Structurally, they contain functional regions such as the DNA-binding domain and transcriptional regulatory domain, among which the amino acids at positions 14 and 19 of the AP2/ERF domain are key markers for classification. In terms of classification, both the ERF and DREB subfamilies can be further divided into 6 subgroups. In terms of distribution, the number of members of this family varies significantly among different plants, and the number of ERF subfamily members in dicotyledonous plants is usually more than that in monocotyledonous plants. The functional mechanism of ERF in biotic stress response is emphatically elaborated as follows. On the one hand, it enhances plant resistance to pathogens by activating disease-resistant genes such as PR and PDF1.2; on the other hand, ERFs containing the EAR motif can act as negative regulators to inhibit the expression of target genes. At the same time, this article summarizes the research status of peanut ERF, including family identification (our research group identified 76 ERF family members in cultivated peanuts in 2022), verification of stress resistance functions (such as AhERF008 and AhERF019 can enhance abiotic stress tolerance), and current limitations (such as insufficient systematic analysis and unclear regulatory mechanisms). Finally, the future research directions are prospected, proposing that multi-omics and gene editing technologies should be combined to analyze the ERF-mediated stress resistance network, so as to provide a theoretical basis and technical targets for peanut stress resistance molecular breeding and facilitate research on peanut stress resistance engineering.

The transcription profile of Micropterus salmoides against LMBV infection was analyzed to reveal the immune response mechanism of Micropterus salmoides in response to LMBV infection. The head kidney tissues of largemouth bass infected with LMBV 72h were analyzed by transcriptome sequencing using Illumina NovaSeq 6000 sequencing platform. A total of 5953 differentially expressed genes (DEGs) were obtained, of which 1704 were up-regulated and 4249 were down-regulated. Through GO function annotation, DEGs were mainly related to single-organism process, metabolic process, membrane, cell, binding, catalytic activity, etc. The KEGG enrichment analysis showed that DEGs were mainly enriched in metabolic pathways such as glycolysis, primary bile acid biosynthesis. There were 4 immune response-related signaling pathways enriched including cytokine-cytokine receptor interaction, ECM-receptor interaction, phagosome and intestinal immune network for IgA production. The immune-related increased DEGs were cxcr4, il10, mrc, ncf4, itgb2, il27, ccl25, etc. while the decreased degs were cxcl12, tgfb3, il20ra, col4a5, itgb1, etc. PPI analysis showed that itgb1, itga8 and itgb6 were the hub genes of immune-related DEGs. This study analyzed the transcription profile of largemouth bass infected with LMBV to provide a theoretical basis for the molecular mechanism and disease prevention of largemouth bass immunity against LMBV.

To evaluate the field control effects of several chemical control agents on potato late blight in Matou Town of Tancheng County, Linyi City, Shandong Province, 687.5 g/L fluopyram-penoxifen wettable powder, 72% mancozeb-manganese zinc, 42.8% fluopyram-iprovalent wettable powder, and 10 billion/g Bacillus subtilis biological preparation were selected, and a control group and treatment groups were set up, and the incidence index, control effect, and yield were monitored. The results showed that the 687.5 g/L fluopyram-penoxifen wettable powder had a significant control effect on the disease. In 2020, the incidence index was 3.21, 5.13, and 6.02 after three applications of the treatment, with an average control effect of over 80% and a maximum of 85.14%, and an increase in yield of 27.9%. In 2021, the incidence index was 2.95, 4.75, and 5.45 after three applications of the treatment, with an average control effect of over 84% and an increase in yield of 26.0%. The 42.8% fluopyram-iprovalent wettable powder had a slightly lower control effect but was better than other pesticides. The importance of selecting control agents based on regional specific conditions was emphasized, and fluopyram-penoxifen wettable powder was recommended as the preferred control agent for late blight of potato in the study area. The research results provide scientific control guidelines for farmers.

Further understanding the genetic regularity of rice harvest index in different segregating generations is beneficial to provide theoretical references for rice breeding based on the rice harvest index. In this research, isolated descendants from four distinct rice hybrid combinations were selected based on their harvest index. And then the coefficient of variation for main agronomic traits and the selection efficiency regarding the harvest index were subsequently compared and analyzed across different generations. The characteristics such as SPAD value at booting stage, plant height, and panicle length exhibited stability among different rice materials. In contrast, there was considerable variability in sword leaf length, thousand-grain weight, seed setting rate, and harvest index among hybrid rice progeny; however, these four traits remained stable in their parental lines. Notably, aboveground biological yield and panicle number per plant displayed significant variation both in hybrid parents and their offspring. The generalized heritability of the harvest index for F₃, F₃ lines and F₄ lines across all tested hybrid combinations exceeded 97%. Furthermore, selection efficiency for the harvest index in F₄ lines derived from ‘Hongxin-1S/Yuxiangyouzhan’, ‘Hongxin-1S/FL478’, and ‘Hongxin-1S/HD-YX’ surpassed that observed in their respective F₃ and F₃ lines; thus indicating a pronounced effect of single-plant selection based on harvest index within these three hybrids. Conversely, single-plant selection among offspring of ‘Hongxin-1S/Guanghong 3’ with high harvest indices did not enhance selection efficiency. It was effective to select individual plant with elevated harvest index for most of the tested rice materials from F₂ generation, which could significantly improve the selection efficiency of harvest index.

To further understand the production characteristics and applicable value of the wheat variety ‘Hemai 26’, its yielding ability, yielding stability and adaptability as well as resistibility and quality characteristic were analyzed with ‘Zhoumai 18’ as the contrast using the regional tests of wheat varieties in southern Huang-Huai Wheat Region consisted of institutions and enterprises during 2016-2018 and production tests in 2018-2019. The results showed that the yield of ‘Hemai 26’ increased by 4.5%, 11.53% and 5.28% respectively compared with that of ‘Zhoumai 18’ during 2016-2019, and reached a significant level. Coefficient of variation (CV) of ‘Hemai 26’ was slightly smaller, the high stability coefficient (HSC) was larger and adaptability was higher than that of ‘Zhoumai 18’. The average yield of each experimental variety showed linear regression with the average yield of ‘Hemai 26’ in different test sites, and the regression coefficient tended to 1. ‘Hemai 26’ has strong lodging resistance and general disease resistance. ‘Hemai 26’ has good grain uniformity, high grain bulk density and good quality. ‘Hemai 26’ has outstanding yielding ability, good yielding stability, wide adaptability and excellent quality, which has good promotion and utilization value in Southern Huang-Huai Wheat Region.

The development of dye industry makes the treatment of dye waste liquid a challenge for environmental protection. Due to the high specific surface area and rich surface functional groups, biochar has excellent performance in adsorption of waste liquid dye substances, which makes it an efficient, economical and environmentally friendly adsorption material. This paper reviewed the adsorption mechanism and influencing factors of biochar in the dye adsorption process. It was pointed out that: (1) the adsorption mechanism of biochar on dye waste liquid including hydrogen bonding, π-π interaction, electrostatic interaction, coordination and ion exchange, jointly determined the interaction and adsorption efficiency of biochar and dye molecules. (2)The factors affecting the adsorption capacity of biochar included the raw materials, modification methods and preparation processes of biochar. (3)The properties of waste liquid, such as solution pH and ion concentration, also affected the adsorption capacity of biochar for dyes. Finally, the problems in the current biochar adsorption research, such as the single research object, the process and cost, and the impact on environment, had yet to be solved. This paper provides a reference for subsequent research to promote the practical application of biochar in the treatment of dye waste liquid.

To establish an efficient and sustainable RNAi breeding control system, this paper summarizes the application potential of RNAi technology in crop disease and pest resistance, reviews the current research and development status of transgenic RNAi crops, and analyzes the design strategies and synergistic resistance mechanisms of multi-target tandem RNAi. The key points of elaboration include the "dsRNA/microRNA stable expression system", the "combined control model of complex pests and diseases (wheat scab - aphids, cotton wilt - cotton aphids)", and the "precise gene intervention approach for delaying resistance evolution", etc. It is pointed out that low delivery efficiency, poor environmental stability of dsRNA and high production cost remain the bottlenecks for large-scale application. This paper proposes that through the precise release technology of nano-carrier-plant symbiotic delivery, tandem expression of multi-gene silencers, and combined with ecological balance monitoring, the coordinated management of multiple pests and diseases can be achieved within 5 to 10 years. It is believed that this system will promote the transformation of agriculture towards a sustainable model of "precise genetic intervention + ecological balance maintenance", providing key support for global food security.

To accurately assess the levels of heavy metal pollution, health risks and pollution sources in the surface soil of orchards in Qingyang City, the research team collected 16 surface soil samples at 0-40 cm soil layer from orchards in Qingyang City. The concentrations of chromium (Cr), cadmium (Cd), copper (Cu), zinc (Zn), lead (Pb), arsenic (As) and mercury (Hg) were determined. Based on the single factor pollution index, the Nemerow comprehensive pollution index, geo-accumulation index, potential ecological risk index and health risk appraisal model were used to evaluate the degree of heavy metal pollution and health risks. In addition, principal component analysis was applied to analyze the sources of heavy metal pollution. The results indicated that the soil of orchards in Qingyang City was severely polluted by cadmium (Cd), reaching a moderate pollution level. The geo-accumulation index for mercury (Hg) was greater than 5, indicating extreme pollution, while the geo-accumulation indices for other heavy metals were all below 0, indicating no pollution. The potential ecological risk index (RI) for the seven elements ranged from 1694.28 to 5158.06, indicating extreme pollution. The total non-carcinogenic risks for young adults (aged from 18 to 40) and middle-aged adults (aged from 41 to 60) were 0.12 and 0.09, respectively, indicating no non-carcinogenic risk to local orchard farmers from soil heavy metals. However, the total carcinogenic risks for young adults and middle-aged adults were 9.21×10^-5 and 7.45×10^-4, respectively, exceeding the acceptable level (1×10^-6), indicating a carcinogenic risk from soil heavy metals to local orchard farmers. Source analysis suggested that the main sources of soil heavy metal pollution may be a combination of traffic and plastic mulch, pesticides, fertilizers, and atmospheric deposition.

This study aims to systematically summarize the application of maize live haploid technology in maize molecular breeding, with focus on exploring the selection methods, formation mechanisms, identification methods, doubling methods, and application in population improvement of maize live haploid high-frequency induction lines, providing reference and guidance for the large-scale application of live haploid breeding technology. This study used a literature review method to summarize the relevant research results and practical experience on maize haploid technology, and analyzed the advantages and disadvantages of various methods in practical applications. The research results indicated that maize live haploid technology played an important role in breeding excellent inbred lines and population improvement. Through high-frequency induction line breeding, a large number of excellent haploid plants had been successfully obtained; the identification and doubling methods effectively improved haploid formation and stability. The live haploid technology had shown significant doubling effects in population improvement, providing new ideas and methods for maize breeding. In summary, maize live haploid technology is an efficient and rapid breeding method with broad application prospects. In the process of corn breeding, combining live haploid technology can accelerate the pace of quality improvement and yield increase, providing strong support for the healthy development of the corn industry.

Climatic factors are the main factors affecting crop growth and development, and have a significant impact on crop growth, yield, and quality. In order to explore the characteristics of climate factor changes in northern Henan and their impact on winter wheat production, this study used agrometeorological observation data of winter wheat from 1984 to 2022 in Qinyang County, Henan Province, combined with meteorological observation data from the same period, and used methods such as linear tendency estimation, Mann Kendall test, HP filtering, and correlation analysis to study the quantitative impact of climate factor changes and climate yield during the growth period of winter wheat. The results showed that from 1984 to 2022, the temperature during the growth period of winter wheat showed a significant fluctuation and upward trend, with a climate tendency rate of 0.35℃/10a (P<0.05). There were obvious seasonal changes in temperature during the growth period, and a sudden change in temperature occurred in 1994. In the past 39 years, the accumulated temperature at ≥0℃ had increased, with a climate tendency rate of 53.5℃/10a (P>0.05), and the mutation points were in 2010 and 2021. The precipitation and sunshine hours during the growth period showed a fluctuating decreasing trend, with the linear tendency rates of 13.4 mm/10a (P>0.05) and -43.0 h/10a, respectively. Affected by climate change, the duration of winter wheat growth season was significantly shortened, and the yield was greatly affected by climate factors. Within a certain temperature range, the climate yield of winter wheat increased with the increase of temperature. For every 1℃ increase in temperature, the climate yield increased by 14.53 g/m². The suitable threshold for accumulated temperature of ≥0℃ during the growth period was 2281℃. There are differences in the impact of climate factors on the climate yield of winter wheat in different growth periods. The sunshine hours from tillering to over wintering, booting to heading, booting to heading temperature, heading to flowering, and precipitation throughout the entire growth period are key factors affecting winter wheat yield. This study can provide important references for regional winter wheat response to climate change and agricultural scientific decision-making.

Epigenetic regulation refers to the heritable control of gene expression without alterations in the DNA sequence, achieved through mechanisms such as DNA methylation, histone modification, RNA modification, chromatin remodeling and non-coding RNAs. Epigenetics provides an additional and flexible source of trait variation for horticultural crop improvement, opening innovative avenues for breeding new varieties capable of addressing challenges like climate change adaptation, disease and pest resistance, and quality enhancement. This review systematically synthesizes current research progress on the epigenetic regulation of key agronomic traits in horticultural crops and summarizes relevant breeding tools and methods developed for their use in horticultural crop breeding. It aims to provide a theoretical reference for further understanding the epigenetic basis of the formation of horticultural crop traits, and provide a theoretical basis and technical support for the promotion and application of epigenetic breeding in horticultural crops.

This study aims to systematically understand the flowering biological characteristics of 16 citrus rootstock resources, including trifoliate orange and citrange, and to provide references for rootstock germplasm creation and new variety breeding. 16 collected trifoliate orange and citrange resources were used as materials. The phenological stages were observed, and the characteristics of floral organs, including bud color, petal number, petal length and width, anther number, filament number, filament color, and stigma number, were compared. Additionally, pollen quantity, pollen grain size, pollen viability, and pollen germination ability were measured using an optical microscope and in vitro pollen germination method. Finally, a comprehensive evaluation of flowering biological characteristics was conducted using the weighted membership function method. (1) The results showed that trifoliate oranges and most of citrange resources were deciduous trees. Citrange trees exhibited a more erect growth habits and reached phenological stages slightly later than trifoliate oranges. Both trifoliate oranges and citrange primarily exhibited pale purple filaments, with most specimens having 20-24 anthers and filaments. However, the petals of trifoliate oranges were generally smaller than those of citrange. (2) Significant differences existed between trifoliate oranges and citrange in terms of pollen viability, pollen quantity, and pollen grain size. Trifoliate oranges generally exhibited higher pollen staining vitality and pollen germination rates compared to citrange. (3) Utilizing the weighted membership function method, trifoliate oranges such as common small-leaf trifoliate orange, M-3 and citrange Dayong No.3 were identified as rootstocks with superior pollen fertility. These results provide reference for evaluating and utilizing rootstock resources, as well as for interspecific and intraspecific hybridization breeding within the citrus genus to create new citrus rootstock germplasm.

By identifying the distribution characteristics of heavy metal elements in Yuanmou County, we can understand the local heavy metal sources and environmental ratings, objectively grasp the environmental problems existing in the soil in this area, put forward scientific and reasonable suggestions for agricultural development and environmental governance, and improve the level of land management and environmental monitoring in this area. The method of combining traditional geochemistry and soil science was used, and the sampling was carried out according to the relevant standards of 1:250000 land quality geochemical survey. SPSS, Excel, GeolPAS.V4.5, ArcGIS10.8 and other software were used for data modeling, result integration and map production. The results showed that heavy metal elements As, Cd, Cr, Cu, Hg, Ni, Pb and Zn were existed in the surface soil of Yuanmou County, in which Cd was enriched on the surface, and the anisotropy of Cd and Hg was higher than that of other elements. The overall distribution of Cu was balanced, with local characteristics of enrichment and depletion. Cr was highly correlated with Ni and weakly negatively correlated with Pb. There were three main sources of heavy metals: mainly rich in Zn and As, mainly rich in Cr, Ni and Cu, and mainly rich in Hg. In the comprehensive grade assessment of surface soil environment, the risk-free area was 1873 km², accounting for 92.77%, and the risk-controlled area was 146 km², accounting for 7.23%. The area was concentrated in Jiangyi Town, Guanyuan Town and Pingtian Town, and scattered in other areas, with no high risk area. The distribution of heavy metals As, Cr, Ni, Pb and Zn in Yuanmou County was mainly controlled by the parent material, Cu and Cd were controlled by the parent material, continuous weathering and human activities, and Hg was mainly controlled by human activities. There was no high-risk area in Environmental rating, and the risk controllable areas were mainly affected by Cu and Cd, among which the Cd risk controllable areas were highly consistent with the concentrated areas of agricultural development, and the application of relevant pesticides should be reasonably controlled and monitored in the later stage. Hg wasn’t at risk in the whole region, but there had been a slight enrichment trend in the surface soil, which required a late warning to avoid pollution.

To investigate the antifungal activity of essential oils (EOs) derived from A. tatarinowii and A. argyi, as well as their principal components, we assessed the antifungal efficacy of both EOs against four pathogenic fungi using the Oxford Cup method. Additionally, EO constituents were analyzed through gas chromatography-mass spectrometry (GC-MS). Subsequently, the minimal inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) were determined through a 96-well plate assay. The results showed that both EOs at a concentration of 50 mg/mL exhibited significant inhibitory effects on Colletotrichum gloeosporioides, followed by Fusarium oxysporum and Fusarium solani. GC-MS analysis revealed that A. tatarinowii EO contained 34 compounds, with β-asarone and α-asarone identified as the primary constituents. In contrast, A. argyi EO comprised 98 compounds, among which caryophyllene, β-caryophyllene, eucalyptol, and myrtenal constituted a substantial proportion. Furthermore, the MIC of β-asarone and α-asarone were found to be comparable to that of hymexazol. When utilized in combination with hymexazol, the two EOs demonstrated an additive impact on Fusarium oxysporum in the pairwise combination experiment. Overall, the EOs and their main components from A. tatarinowii and A. argyi exhibited robust antifungal activity against four fungal pathogens, indicating potential for further research.

To study the soil carbon sequestration potential, a long-term monitoring site of wheat and maize double-cropping farmland in Xiangcheng County, Henan Province was selected as the research object. The Century model was used to simulate the dynamic change process of soil organic carbon during the monitoring period (2010-2023), and the applicability of the Century model was verified by comparing with the measured data. At the same time, one basic agricultural management measure and four optimized agricultural management measures were selected to simulate and predict the soil carbon sequestration potential in the next 20 years (2024-2043) by using the Century model. The simulation results showed that during the monitoring period, soil organic carbon density at the monitoring sites increased by 0.31 mg/hm² annually, showing a “carbon sink” status, mainly due to the increase of nitrogen fertilizer application rate. The results of Century model validation found that the model had good applicability in simulating and predicting the dynamic change of soil organic carbon. Under the condition of basic agricultural management measures, the soil carbon sequestration potential was low, and the state was weak and stable. Under the condition of agricultural management measures combining less tillage and 50% straw returning, the soil carbon sequestration potential was the highest, which was the most effective agricultural management measure. Therefore, the implementation and popularization of optimized agricultural management measures in the wheat and maize double-cropping farmland area of Henan Province is an important means to enhance soil carbon sequestration capacity, which is of great significance for alleviating greenhouse effect and realizing national food security.