Under field condition, let five treatments, T1 no N fertilizer、 T2 chemical fertilizer、 T3 controlled-release urea 、T4 controlled-release urea 90% 、T5 controlled-release urea 80%. A study on the effect of controlled release nitrogen fertilizer on plant and soil N concentration and yield of rice in cold region. The results showed that controlled release nitrogen fertilizer promote soil ammonium and soil nitrate nitrogen contents of whole growth stage, soil ammonium contents showed increased first decline, Ear - milk stage content minimum; Nitrate nitrogen increased at first, then decreased, the highest content heading stage. Different nitrogen treatment plant total nitrogen gradually raised and maximum maturity, and T3 treatment the highest. Compared with a T1 increased by 12.45%-18.41%.Controlled release nitrogen treatment yield higher than conventional fertilization treatment, and T4 treatment, the highest yield reached 7944.44 kg/hm2, compared with T2 treatment increased by 7.3%, compared with the T1 treatment increased by 19.0%, the difference reached significant level. In addition, controlled release nitrogen fertilizer application improves the soil and plant nitrogen content. Promote the rice plant dry matter accumulation, so as to improve crop yield.