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Identification of Characteristics and SSR Molecular Markers of A New Flue-cured Tobacco Strain ‘Shiliuyan’
LI Xianfu, YANG Hui, AN Xueqin, WANG Chenzijun, DAI Xiumei, ZHANG Jiankui
Identification of Characteristics and SSR Molecular Markers of A New Flue-cured Tobacco Strain ‘Shiliuyan’
The objective is to breed new varieties of flue-cured tobacco, improve the yield and quality of tobacco leaves and meet the needs of tobacco production. A new flue-cured tobacco strain ‘Shiliuyan’ was selected from the local tobacco varieties planted in Shiqian County of Guizhou Province. Using the main commercial varieties ‘K326’ and ‘Yunyan87’ as control, the botanical characters, agronomic traits, economic characters, appearance quality, physical indexes, chemical components and sensory evaluation quality of tobacco leaves of ‘Shiliuyan’ were evaluated through field tests and laboratory quality tests of three consecutive years. The SSR molecular markers of ‘Shiliuyan’ were also established. The results showed that the number of effective leaves per plant of ‘Shiliuyan’ was 24, 3 leaves more than ‘K326’ and 5 leaves more than ‘Yunyan87’, respectively. The economic indicators of ‘Shiliuyan’, including the yield of 2233.4 kg/hm2, average price of 24.47 Yuan/kg, production value of 54434.2 Yuan/hm2, the rate of superior grade leaves of 51.11%, the rate of superior and middle grade leaves of 94.81%, were better than those of ‘K326’ and ‘Yunyan87’. The color of tobacco leaves of ‘Shiliuyan’ was lemon yellow, and the strain had good appearance quality, good coordination of chemical components and better sensory evaluation quality than those of the control. The SSR molecular marker primer PT3024 and PT40021 could stably amplify specific bands among ‘Shiliuyan’, ‘K326’ and ‘Yunyan87’. In brief, ‘Shiliuyan’ is a new flue-cured tobacco strain with high yield and good quality of tobacco leaves.
Flue-cured Tobacco (Nicotiana tabacum L.) / Selection and Breeding / ‘Shiliuyan’ / SSR Molecular Marker {{custom_keyword}} /
表1 植物学性状和农艺性状表现 |
品种(系) | 株型 | 叶形 | 叶耳 | 叶面 | 叶尖 | 叶缘 | 株高/ cm | 茎围/cm | 节距/cm | 有效 叶数 | 腰叶长/cm | 腰叶宽/cm | 大田生育期/d |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
狮柳烟 | 筒型 | 长椭圆形 | 较小 | 较皱 | 急尖 | 较平 | 125 | 9.5 | 5.0 | 24 | 86 | 40 | 130 |
K326 | 筒型 | 长椭圆形 | 较小 | 较皱 | 渐尖 | 波浪状 | 106 | 9.2 | 4.7 | 21 | 78 | 39 | 130 |
云烟87 | 塔型 | 长椭圆形 | 中等 | 较皱 | 渐尖 | 波浪状 | 115 | 8.8 | 5.1 | 19 | 85 | 37 | 125 |
表2 经济性状表现 |
年份 | 品种(系) | 产量/(kg/hm2) | 产值/(元/hm2) | 均价/(元/kg) | 上等烟率/% | 中等烟率/% |
---|---|---|---|---|---|---|
2016 | 狮柳烟 | 2358.9 | 56621.4 | 24.00 | 45.14 | 51.71 |
K326 | 1782.9 | 35631.0 | 19.99 | 31.15 | 51.90 | |
云烟87 | 1640.4 | 32090.9 | 19.48 | 30.24 | 47.37 | |
2017 | 狮柳烟 | 2372.6 | 55293.6 | 23.31 | 49.02 | 42.55 |
K326 | 1755.3 | 38048.4 | 21.68 | 44.70 | 36.71 | |
云烟87 | 1318.1 | 27766.2 | 21.07 | 38.79 | 45.53 | |
2018 | 狮柳烟 | 1968.8 | 51387.6 | 26.10 | 59.18 | 36.84 |
K326 | 1682.3 | 42207.0 | 25.09 | 58.80 | 40.25 | |
云烟87 | 1387.5 | 35145.5 | 25.33 | 57.38 | 35.61 | |
平均 | 狮柳烟 | 2233.4 | 54434.2 | 24.47 | 51.11 | 43.70 |
K326 | 1740.2 | 38628.8 | 22.25 | 44.88 | 42.95 | |
云烟87 | 1448.7 | 31667.5 | 21.96 | 42.14 | 42.84 |
表3 原烟的外观品质和物理特性 |
品种(系) | 成熟度 | 身份 | 色泽 | 单叶质量/ g | 含梗率/ % | ||||
---|---|---|---|---|---|---|---|---|---|
油分 | 厚度/µm | 叶片结构 | 颜色 | 光泽 | |||||
狮柳烟 | 成熟 | 稍有 | 0.46 | 疏松 | 柠檬黄 | 中 | 9.5 | 34.0 | |
K326 | 成熟 | 有 | 0.53 | 疏松 | 橘黄 | 中 | 10.0 | 33.7 | |
云烟87 | 成熟 | 有 | 0.46 | 疏松 | 橘黄 | 中 | 9.3 | 33.0 |
表4 原烟的化学成份 |
品种(系) | 总糖/ % | 还原糖/ % | 总植物碱/ % | 总氮/ % | K2O/ % | Cl/ % | 糖碱比 | 氮碱比 | 钾氯比 | 石油醚 提取物/% | 总粒相物/ mg |
---|---|---|---|---|---|---|---|---|---|---|---|
狮柳烟 | 30.89 | 22.18 | 2.14 | 1.62 | 2.73 | 0.36 | 10.36 | 0.76 | 7.58 | 6.02 | 28.3 |
K326 | 35.7 | 25.7 | 2.00 | 1.42 | 2.30 | 0.34 | 12.85 | 0.71 | 6.76 | 4.56 | 32.3 |
云烟87 | 32.9 | 24.0 | 2.21 | 1.52 | 2.48 | 0.40 | 10.86 | 0.69 | 6.20 | 5.65 | 33.4 |
表5 原烟的感官评吸评价 |
品种(系) | 香型 | 劲头 | 烟味浓度 | 香气质 | 香气量 | 余味 | 杂气 | 刺激性 | 燃烧性 | 灰色 | 评吸总分 | 总体评价 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
狮柳烟 | 中间香型 | 适中 | 中等+ | 12.13 | 16.58 | 20.30 | 13.80 | 8.91 | 3.50 | 4.0 | 79.2 | 好 |
K326 | 中偏清香型 | 适中 | 中等 | 11.88 | 16.09 | 19.80 | 13.61 | 8.79 | 3.50 | 3.0 | 76.7 | 较好 |
云烟87 | 中间香型 | 适中 | 中等 | 11.76 | 16.28 | 19.55 | 13.31 | 8.32 | 3.50 | 3.0 | 75.7 | 较好- |
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