
Polysaccharide Extraction in Lactuca Satya L. and Its Antihyperlipidemic Activity
Yang Fengrong, Gao Jianping, Li Chunyan
Polysaccharide Extraction in Lactuca Satya L. and Its Antihyperlipidemic Activity
The aim is to establish and optimize the extraction process of polysaccharides from Lactuca Satya L. and to explore the in vitro hypolipidemic activity of the polysaccharides. Lactuca Satya L. was used as material, polysaccharides was extracted by water/ethanol method. The polysaccharide extraction process of Lactuca Satya L. was established by back propagation (BP) neural network and orthogonal design. The in vitro hypolipidemie of polysaccharide in Lactuca Satya L. was studied by in vitro binding of cholates. The optimal condition for Lactuca Satya L. polysaccharide extraction was at 89℃ with solvent amount of 44 times in 1.6 h and 4 extracting times. The polysaccharides in Lactuca Satya L. and their alcohol-precipitated fractions had better cholate binding capacity with sodium taurocholate (STC), glychocholic acid sodium salts (SGC), sodium cholate (SC). BP neural network combined with orthogonal test could reduce experiment times effectively, analyze the change rules of Lactuca Satya L. polysaccharide extraction factors, and obtain the optimal extraction parameters. The hypolipidemic mechanism of Lactuca Satya L. polysaccharide might be the combination of cholic acids in intestine, which affected the lipids absorption.
Lactuca Satya L. / Polysaccharide / extraction process / Orthogonal Design / BP Neural Network / Hypolipidemic {{custom_keyword}} /
[1] |
徐宁. 茜草、生菜子等中挥发油的提取分离和化学研究[D]. 通辽:内蒙古民族大学, 2006.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[2] |
边学义, 赵士林, 高清溢, 等. 生菜籽及红糖对家兔实验性高血脂症及动脉粥样硬化防治的初步研究[J]. 锦州医学院学报, 1982(2):20-23.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[3] |
肖志杰. 生菜籽提取物对小白鼠中枢神经系统抑制作用的实验研究[J]. 锦州医学院学报, 2005(3):15-17.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[4] |
肖志洁, 王维芝, 梁日昕. 生菜籽提取物对实验性心律失常作用的初探[J]. 锦州医学院学报, 1991(5):285-287.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[5] |
吴雅清, 许瑞安. 降血脂多糖的研究进展[J]. 中国中药杂志, 2018, 43(17):3451-3459.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[6] |
董文南, 李克招, 张文婷, 等. 多糖降血糖作用及其机制研究进展[J]. 中国实验方剂学杂志, 2019(19):219-225.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[7] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[8] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[9] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[10] |
Bletilla striata polysaccharides (BSP) have attracted extensive research interest due to their potential medical application. Herein, infrared-assisted technique is employed for the first time to extract BSP from B. striata (Thunb.) Reichb.f. based on a Box-Behnken design (BBD) and response surface methodology, with the optimum extraction parameters as follows: 75°C extraction temperature, 2.5h extraction time; and water to solid ratio (53ml/g). Based on it, 43.95±0.26% yield of crude BSP was obtained. Subsequently, crude BSP was further decolorized, deproteinized, freeze-dried, and purified by a DEAE-52 cellulose column. Furthermore, the micro-structure and a triple-helical structure of BSP were characterized. Fourier transform infrared spectra confirmed its polysaccharide characterization via typical peaks. In addition, the significant in vitro antioxidant profiles of BSP were demonstrated by superoxide anion radical-scavenging assay, hydroxyl radical scavenging assay, DPPH free radical scavenging activity and chelation of ferrous ions. Taken together, this study provide an efficient extraction technique for BSP as a promising natural antioxidant.Copyright © 2016 Elsevier Ltd. All rights reserved.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[11] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[12] |
吴雅清, 许瑞安. 降血脂多糖的研究进展[J]. 中国中药杂志, 2018(17):3451-3459.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[13] |
黄越, 周春晖, 黄惠华. 不同提取方法猴头菇粗多糖的表征及其抗氧化活性的比较[J]. 食品工业科技, 2017, 38(3):80-86.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[14] |
廖国会, 龙家寰, 秦立新, 等. 松乳菇多糖提取工艺优化及抗氧化活性评价[J]. 食品工业科技, 2018, 39(4):123-130.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[15] |
孟楠, 樊振江, 高雪丽. 纤维素酶法提取黄秋葵多糖的工艺优化[J]. 食品工程, 2014(4):14-17.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[16] |
万宇俊, 殷军艺, 聂少平, 等. 微波提取对胡萝卜中的多糖基本结构特征及固体形貌的影响[J]. 食品科学, 2017, 38(7):1-5.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[17] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[18] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[19] |
瞿昊宇, 陈光宇, 何群, 等. 药膳五汁饮中阿魏酸和多糖含量测定研究[J]. 亚太传统医药, 2019(5):77-81.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[20] |
丁钢强, 于村, 张双凤, 等. 食品多糖含量不同测定方法的研究[J]. 实用预防医学, 2000(5):325-327.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[21] |
王鑫, 姜红, 张超, 等. BP神经网络结合正交试验优化安痒汤提取工艺[J]. 亚太传统医药, 2016, 16(16):18-21.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[22] |
唐静, 袁秀芝. BP神经网络结合遗传算法优化黄芪多糖提取工艺[J]. 中国医院药学杂志, 2018, 38(15):1609-1611.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[23] |
邵方元, 付腾飞, 薛兴阳, 等. 正交试验和BP人工神经网络模型优化胰蛋白酶提取鱼腥草多糖的工艺[J]. 中国实验方剂学杂志, 2013(11):14-17.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[24] |
辛勋, 陆红云. 紫外分光光度法测定速效伤风胶囊中的胆酸含量[J]. 中国药业, 2000, 9(1):36-37.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[25] |
于美汇, 赵鑫, 尹红力 等. 碱提醇沉黑木耳多糖体外和体内降血脂功能[J]. 食品科学, 2017, 38(1):232-237.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[26] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
{{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
/
〈 |
|
〉 |