ZHANG Jing, LIU Lina, YANG Baoming, WANG Yongfen, HE Ping, XU Shengtao, YIN Kesuo, LI Shu, BAI Tingting, LI Yongping, LI Xundong, ZHENG Sijun
In order to fully explore and utilize the functional active substances of the green peel of different ecological banana varieties, an ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-MS/MS) method was established to quantitatively analyze main phenolic acids in the green peel of various banana varieties. Agilent ZORBAX Extend-C18 column (2.1 mm×100 mm, 3.5 μm) was used, gradient elution was conducted with methanol (A)-0.2% formic acid aqueous solution (B), flow rate of 0.3 mL/min, injection volume of 5 μL, column temperature of 35℃. Under the negative ion scanning mode and the multi-reaction monitoring mode, the electrospray ionization source was detected. The separation effect of seven phenolic acids (gallic acid, quinic acid, protocatechuic acid, catechin, chlorogenic acid, ferulic acid and rutin) was good, and the mass scores showed good linear correlation in the range of 1-200, 1-2000, 1-200, 1-200, 1-200, 2-200, and 1-500 μg/L (r2>0.9992), respectively, the average recovery (n=6) was 84.37%-92.17%, and RSD≤10.96%. The established UPLC-MS/MS method had the characteristics of accuracy, sensitivity, rapidity and good repeatability, and could be used for the simultaneous determination of the content of the seven phenolic acids in green banana peel. The content of main phenolic acids in the green peel of different ecological banana varieties was significantly different. The phenolic acid content in green peel of non-dessert bananas was significantly higher than that of dessert bananas, and the phenolic acid content in green peel of non-dessert banana ‘Pahang’ was the highest, which was 2.12 mg/g.