AP2/ERF（APETALA2/ethylene responsive factor） is one of the largest transcription factor （TF） families in plants， which contains at least one specific AP2 domains composed of 60-70 highly conserved amino acids. Depending on the number and sequence similarity of AP2 domains， this family can be classified into five subfamilies： AP2 （APETALA2）， DREB （dehydration-responsive element binding proteins）， ERF （ethylene-responsive factor）， RAV （related to AB13/VP）， and Soloist. AP2/ERF TFs regulate their expression by binding to target genes through YRG and RAYD conserved elements in the AP2 domain. At present，AP2/ERF TFs have become a hot candidate gene for studying plant stress resistance mechanisms and biosynthesis of active ingredients. More and more AP2/ERF families and their members have been reported. In this review， we summarized the latest research achievements on plant AP2/ERF family， including the structural characteristics and classification， and the research progress of AP2/ERF TFs involved in regulation of plant secondary metabolites synthesis， participation in biological and abiotic stress response was mainly introduced.Meanwhile， possible hot research topics and fields of AP2/ERF were proposed，which may provide a reference for further mining and utilization of such transcription factor genes for plant genetic improvement and germplasm innovation.

The variation coefficient analysis， genetic diversity analysis， correlation analysis， principal component analysis and cluster analysis of 647 island cotton germplasm resources were carried out in order to screen more diverse types of island cotton germplasm resources for parent selection and variety breeding in the future. The variation range of quantitative index of 647 sea island cotton germplasm resources was 2.4608%~36.4320%， indicating the rich diversity among sea island cotton germplasm resources. The number of stem hairs， leaf color， leaf hairs， petal basal spot size， main stem hardness， fruit branch type and style length of island cotton germplasm resources were variable， and these external descriptive traits could be directly used for the improvement of plant morphology. Genetic diversity analysis of quantitative indicators showed that the diversity of indicators reflecting fiber quality was more abundant than that reflecting yield， and germplasm resources could be used for improving fiber quality and maturity. Correlation analysis revealed a significant correlation between different quantitative traits. Among them， the first fruit branch node was significantly negatively correlated with the average length of the upper half， the uniformity index and the breaking strength， the sub-index was significantly negatively correlated with the micronaire value， and the lint percentage was significantly negatively correlated with the average length of the upper half. The above correlation is consistent with previous research results on upland cotton， The complicated interaction mode implied a comprehensive evaluation by integrating multiple datasets in germplasm innovation. The principal component analysis showed that the cumulative contribution rate of the first five eigenvalues reached 75.761%. The first principal component was related to fiber quality， the second principal component was related to seed cotton yield， the third principal component was related to elongation， the fourth principal component was related to maturity， and the fifth principal component was related to lint percentage. When the genetic distance was 10， the germplasm resources were divided into 6 groups by cluster analysis. The comprehensive performance of cluster II was better. In actual breeding， targeted selection and improvement can be carried out according to breeding objectives.

Chili（Capsicum annuum L.）crops have high economic value and extensive planting， and most of the chili cultivars in China have high plant type， many branches， easy lodging， not conducive to mechanized production， and the cost of artificial production is rising. With the improvement of agricultural production technology and the increasing shortage of labor， the transformation of traditional agriculture to modern mechanized agriculture is imminent. The proposal of ideal plant type makes plant type regulation a hot spot in genetic breeding， which can provide reference for the analysis of the regulation mechanism of chili plant type. This paper reviews the research results of recent domestic and foreign scholars on the genetic factors and molecular mechanisms of plant type regulation， the biological relationship between plant hormones and plant type， and the influence of the environment on plant type， and puts forward the idea of ideal plant type of chili. Good chili plant type can improve plant production capacity， facilitate management， alleviate labor shortage， and accelerate the process of mechanized production. At present， there are few research reports on the regulation mechanism of chili plant type， so exploring the breeding mechanism and genetic basis of plant type regulation is conducive to providing theoretical support for the creation of good plant type germplasm resources and accelerating the selection and breeding of new varieties， and laying the foundation for the genetic breeding .

Wheat variety Yannong 999 （YN999） shows stably high yield potential with strong environment adaptability. Unlocking its genetic basis and key chromosomal regions underlying high yield performance will provide theoretical support for the further application. In this study， a 55K wheat SNP array was used for genotyping the YN999， its 46 derived varieties （lines） and a natural mapping population containing 243 wheat varieties （lines）. The genetic effects of the key chromosomal segments undergone strong selection was elucidated. The genetic cause of high-yielding potential in YN999 was dissected based on the composition of excellent alleles underlying the three yield components. The characteristics of high thousand kernel weight were preferentially selected and present in the derived varieties （lines）. Genotyping using the wheat 55K SNP array revealed that the average genetic similarity coefficient of YN999 if compared to 46 derived varieties （lines） was 0.87. The genetic contribution of YN999 to its derived varieties （lines） of F₃， F₅， F₆ and F₇ were 84.94%， 86.19%， 86.67% and 87.65%， respectively. A total of 222 segments of YN999 with over 95% transmission rate were detected in the offspring of YN999， and the length of the segment varied from 5.04 Mb to 108.75 Mb， among which 2A contained the longest segment with high frequency selection， being 483.37 Mb， and 7D contained the shortest of 13.84 Mb. A total of 135 identified QTL related to yield traits were coincided with the 222 high-frequency selection regions， with 80， 48 and 7 QTL in the A， B and D genome， respectively. A total of 1195， 267， 790 and 678 significant SNPs， which were correlated with yield per plant， kernel number per spike， 1000-grain weight and spike number per plant， respectively， were detected by single marker QTL analysis using a natural mapping population. Among those， approximately 84.02%， 51.69%， 94.18% and 13.42% alleles contributing to the higher yield performance were identified from YN999. These results indicate that YN999 has enriched the superior alleles of yield per plant and 1000-grain weight， which might be the important genetic basis for the high and stable yield in YN999. This study provided theoretical reference in application of YN999 as key parent in molecular breeding programs， and identification and cloning of the genes with high yield performance.

Faba bean （Vicia faba L.） is an important legume crop in China. Southern China is the autumn planting area of faba bean， where mainly produces large-grained fresh faba bean. The correlation analysis of the seed size-related traits of the faba bean resources from this area was carried out to explore the associated molecular markers applicable for molecular marker-assisted breeding. In this study， phenotype of seed size-related traits in 90 faba bean resources were collected in 2019 and 2020. Sixty-seven pairs of polymorphic SSR markers were used for genotyping， producing a total of 278 alleles with the average alleles of 4.1. The resources were divided into 3 groups by cluster analysis， in which accessions with higher values of seed length， seed width and 100-seed weight are mainly found in the A group. In addition to the population structure analysis， the MLM models （Q+K） of TASSEL software were used to conduct associate studies of seed size-related traits and 67 SSR markers. A total of 50 significantly associated markers （P<0.001） were detected by mixed linear model （MLM） of TASSEL， of which 29 markers had a P-value less than 0.0001. In multiple environments analysis， ICS48 and ICS455 were significantly associated with both grain width and grain weight. According to the association stability and contribution rate， three dominant alleles （ICS48-H1， ICS51-H1 and ICS455-H3） related to seed size were obtained （P<0.0001； r²>18%）. This study will contribute to the molecular marker-assisted selection and molecular design breeding of faba bean in autumn planting areas in China.

Anthocyanins， which are natural pigments and serve as important natural antioxidants scavenging free radicals， are rich in a variety of compounds that are important in health care. Anthocyanins affect the ripening， taste and color of fruits and vegetables， and prevent plants from abiotic and biotic stresses. Therefore， optimizing anthocyanin content is regarded as the breeding goal in many horticultural crops. As the secondary ethylene signaling transcription factors， ethylene response factors （ERFs） respond to plant hormone signaling and can result in feedback regulation， and these genes are known to modulate the process of ethylene regulating anthocyanin biosynthesis via various mechanisms. In terms of the molecular mode， ERFs in regulation of anthocyanin biosynthesis rely on the physical interaction with transcription factors， activating transcription factors， forming regulatory complexes with MBW or directly activating structural gene promoters. This study aims to provide a theoretical basis for further elucidating the mechanism of ERF regulating anthocyanin biosynthesis， and to explore the relationship between the rapid accumulation of anthocyanins and the increase of ethylene release in fruits and vegetables at the late ripening stage.

Senescence，as the final stage of natural development of plants，has an important influence on crop yield. In order to promoting the selection and breeding of new crop varieties and yield improvement， it is important to deeply analyze the regulatory mechanism and influencing factors of premature senescence. Except the stress of natural environment，genetic network of crops is an important factor in regulating plant premature senescence. A variety of metabolic pathways in plants affect the period of senescence onset. Here we reviewed the various physiological，biochemical and yield changes during premature senescence in plants. Premature senescence caused degradation of chlorophyll and other macromolecules，significantly reducd leaf photosynthetic and transportion of nutrients from senescent tissues to young tissues and reproductive organs. The process was accompanied by the accumulation of reactive oxygen species （ROS），decreased the activity of antioxidant enzymes in the cells and upregulated of senescence-associated gene （SAG） expression，which eventually led to premature senescence and reduced plant yield. Premature senescence is a complex and sequential process regulated by multiple genes. We summarized the gene networks regulating premature senescence among different species，and introduced the mechanisms of premature senescence regulation through transcription factor regulation，hormone and protein metabolism. It provides suggestions on the research of premature senescence mechanism and breeding utilization in the future.

Wheat grain， with rich nutrition and various end-uses in markets， provides diets in over one-third of the global human population. However， with the increasing influence of biological and abiotic stresses， such as threats of diseases and pests， environmental damages of drought， high temperature and salinization， the sustainability of global wheat production is under increasing threats. In order to ensure the global food security supply and demands for high quality products， the desirable increases on wheat production and quality require to the constantly developing of new breeding methods and germplasm resources used for wheat breeding. In the past decade， significant progress on plant biotechnologies such as transgenic study and genome editing has been achieved， and gradually applied in wheat genetic improvement. To date， the efficient systems for wheat genetic transformation and genome editing have been established， in which the transformation efficiency for the model genotypes mediated by Agrobacterium is higher than 50% and the editing efficiencies of some target genes via CRISPR/Cas9 reach to 40%-70%. The genotype independency in wheat transformation and genome editing has been overcome almost. Some of wheat traits including disease resistance， stress tolerance， quality feature， yield potential， and growth and development regulation have been modified by using transgenic and gene editing methodologies； many new wheat genetic stocks showing disease resistances to powdery mildew， rusts， scab and yellow mosaic virus， tolerances to pre-harvest sprouting， drought and salt， low gliadin content， high gluten content， male sterility and haploid induction ability were created by the requirement of wheat improvement. This review aims to summarize the latest research progresses on transgene and genome editing in wheat， and to explore the current problems and possible solutions.

The tiller angle is one of the most important traits in the plant architecture of rice， which significantly determines the rice yield. At present， the major tiller angle genes in rice are TAC1 （Tiller Angle Control 1） and TIG1 （Tiller Inclided Growth 1）. It is necessary to further explore new loci and molecular markers to promote ideal plant architecture breeding in rice. In this study， a BC₃F₂ population was developed using large-angled wild rice as the donor and small-angled cultivar Zhenshan 97 as the recipient， and tiller angle separation was observed in the 54th line. QTL mapping of tiller angel was performed using QTL-seq， and a QTL was detected on chromosome 8. TIG1 was identified as the candidate gene through sequence comparison of known genes within the interval. A KASP functional molecular marker was designed based on the causal variation of C?T at -449 bp in the promoter of TIG1. The marker was verified in the mapping population and varieties， and it was confirmed that the KASP marker can accurately identify the genotype of the TIG1 locus. In Geng/JaponicaTIG1 with large angle is dominant， while 61.40% and 38.40% of Xian/Indica varieties carry tig1 with small angle and TIG1 with large angle， respectively. This marker has significant potential utilization value for improvement of rice plant architecture. The development of this KASP marker provides a new tool for molecular marker-assisted improvement of rice tiller angle and is expected to speed up the breeding process for the ideal rice plant architecture.

DELLA proteins are known as negative regulators of gibberellin involved in plant flowering. Eight DELLA genes were identified in soybean genome by sequence alignment with Arabidopsis thaliana（L.） Heynh. DELLA orthologs GmGAI3a has only one GRAS domain，and the other seven DELLA proteins have both DELLA domain and GRAS domain. By performing gene-based association analysis of flowering time in natural population，soybean DELLA haplotypes associating with early-flowering have been detected in soybean accessions collected from the middle and high latitude of China，speculating DELLA genes as negative factors in regulating flowering. CRISPR/Cas9-based editing in soybean hairy root system revealed the editing efficiency at the target sites. Identification of the CRISPR/Cas9 targets of seven DELLA genes provided references for generating stable transgenic DELLA mutants for deciphering their biological functions.

Seed coat color is an important agronomic trait that associates with crop domestication and serves as morphological marker. In mung bean， the seed coat color was related to the content of flavonoids. Cloning and application of seed coat color-related genes becomes of interest in development of new mung bean varieties with improved nutritional properties. In this study， the varieties 'Jilv 9' （black seed coat） and 'Ziyuan 330' （yellow seed coat） were used as parents to generate an F₂ segregating population. The BSA-seq approach was applied for mapping of the genes underlying the seed coat color. The association analysis using integrated SNPs and InDels suggested an interval of 3.26 Mb harboring 324 predicted genes， of which 49 genes were found with non-synonymous mutation and 15 genes were detected with frameshift mutation. By further use of 11 high-quality KASP markers in fine mapping， the candidate interval was finally delimited between KA330 and KA421 in the physical interval of 16，302，330-18，013，421 bp （1.71 Mb） on chromosome 4. The transcriptome data analysis and qRT-PCR expression analysis suggested six differently-expressed candidate genes， of which the LOC106758748 was annotated as a transcription factor MYB90 that was reported with a function in the flavonoid biosynthesis and served as key candidate gene regulating the seed coat color in mung bean. The results of this study can provide a theoretical basis for the cloning and utilization of the genes related to seed coat color in mung bean seed breeding.

As one of the important mechanisms of epigenetic regulation， usually occurs in plant cytosine bases， including CG， CHG and CHH. DNA methylation mainly affects chromatin structure and gene transcription level. DNA methylation plays important roles in transcriptional regulation and maintaining the genome stability. Abiotic stresses affect plant growth and reproduction and ultimately lead to plant death. Based on existing research findings， DNA methylation can induce phenotypic alterations in plants under stress.To cope with abiotic stresses， the change mechanism of DNA methylation level during growth is affected by methylase and demethylase. The signal transduction pathways can change the expression of some stress response genes， thus causing changes in plant morphology， physiology and biochemistry to adapt to adversity. Some genes are upregulated （initiate） or downregulated （close down） in expression in order to assure the adaptive growth and development of plant， thus enabling plants to adapt and resist stress damage to a certain extent. This article reviews the DNA methylation modification and its role in transcriptional regulation， research progress in the growth and development of horticultural plants， and the epigenetic regulation of abiotic stress as well as the problems and prospective of horticultural plants. It provides a reference for the genetic improvement of horticultural plants and deciphering the mechanism of stress resistance.

DNA fingerprinting analysis that enables assigning uniform identity information for each rice germplasm is of great significance for identifying the genetic basis of rice germplasm resources， improving their utilization efficiency and protecting the intellectual property rights of the seed industry in China. In this study， using 5374 rice accessions that have been genotyped by the whole-genome resequencing， two sets of genome-wide DNA fingerprinting standards were established through the selection of reference sample resources， analysis of high-quality SNP loci， and selection of the optimal number of SNPs and SNP combinations. Through principal component analysis and phylogenetic tree analysis， SNPs in the collection of fingerprinting standards 1 and 2 could represent 94，197 high-quality population common SNPs applicable for the population genetic diversity. In addition， population genetic similarity analysis verified the effectiveness of fingerprinting standards 1 and 2 to uncover the genetic similarity identification of rice germplasm resources. This study is expected to provide technical support for the conservation and utilization of rice germplasm resources and the protection of intellectual property rights in the seed industry， and to provide reference for the development of DNA fingerprinting standards for other crops.

Fluorescence in situ hybridization （FISH， fluorescence in situ hybridization） is a powerful tool for molecular cytogenetics studies and is able to authentically allocate particular DNA or RNA sequences on chromosomes. With the development of the genome sequencing technology， the reduction of sequencing cost and the publishment of a large number of species genome information， Oligonucleotide （Oligo） probes based on high-throughput sequencing and reference genome were developed showing the advantages in FISH. In comparison with the traditional probes， Oligo-FISH can further reveal the evolution， inheritance and variation of chromosomes more precisely and deeply in plant evolution.This paper reviews the types and applications of target DNA and fluorescent probes in the development of fluorescence labeling， as well as the types and preparation techniques of oligonucleotide probes， mainly focusing on the origin and development of Oligo-FISH and its application in plants， which plays an important role in the identification of plant chromosomes and plant homologous chromosomes. Since the karyotype of species and genera can be constructed by Oligo-FISH technology， the results of Oligo-FISH can provide guidance for genome assembly of crops in this genus which have no complete genomes. Oligo painting can also solve the problem of fusion and exchange between chromosomes of heteropolyploid species and accurately detect whether there is translocation and heterologous recombination between chromosomes. Therefore， the development of Oligo-FISH technology provides strong support for the assembly of the genome at chromosome level. In the future， Oligo-FISH technology combined with signal amplification technology can overcome the challenge of low Oligo probes with high concentration of repeat sequences in regions， and visualize limited gene regions， such as the detection of promoters or enhancers or the localization of gene segments in transgenes. These studies will make better use of the research results of species genetics and evolution to further ensure， assist and innovate the improvement and development of crop genetics and breeding.

In order to improve the utilization efficiency of naked barley germplasm resources in China， 398 accessions of naked barley from different regions were used to analyze the phenotypic diversity at 18 traits， followed by the analysis of coefficient of variation analysis， diversity index analysis， correlation analysis， principal component analysis and cluster analysis. The results revealed abundant variations among phenotypic traits in different resources. The diversity index of the nine quality traits was 0.66 to 2.06， with an average value of 1.42， and the highest value in plant height， kernels per spike and the smallest value in kernel rows. The traits had 4.71% （kernel color） to 61.03% （heading date） of the coefficient of variation， with an average value of 26.59%. The correlation analysis of nine quantitative traits indicated that spikes per plant， spike length， kernels per spike， thousand kernels weight and setting rate could be deployed as the main target traits for high yield naked barley varieties breeding in the future. The results of principal component analysis showed that the cumulative contribution rate of the five principal component factors was 64.297%. Among them， heading date， plant height， row type and thousand kernels weight were the main factors contributing to the phenotypic differences of naked barley. Combined with the membership function analysis， the comprehensive scores （F value） were calculated. Jiangsuyuanmai No. 33， VII-131， Yumimai， Jiangsu yuan mai No. 58， Jianhutuanliulengzi， Daimaoyuanmai， Jiangsuyuanmai No. 65， Jiangsuyuanmai No. 23， Jiangsuyuanmai No. 20 and Jiangsuyuanmai No. 22 had the highest scores. These materials were divided into four groups by systematic clustering analysis and the clustering results were not strongly correlated with geographic location. These results could provide an important reference for the utilization of naked barley accessions and variety breeding.

The immature embryos of maize are able to form embryogenic calli through tissue culture， and are usually used as explants in genetic transformation. The molecular mechanism in the process of maize embryonic callus formation remains fully illustrated. To reveal the candidate genes that are involved in the callus formation of maize， two types of calli （type I， well growth； type II， poor growth） from F₃ embryos of maize inbred line CAL28 × Zheng58 were harvested for the RNA-seq analysis. 4419 differentially expressed genes （DEGs） were revealed between two types， including 1571 up regulated genes and 2848 down regulated genes. GO enrichment analysis showed that DEGs were mainly enriched in the pathways such as cellular process， catalytic activity， and cell component. KEGG enrichment analysis showed that the enrichment of DEGs were mainly involved in phenylpropanoid biosynthesis and the plant hormone signal transduction pathway. Among the early auxin responses genes， eight AUX/IAA genes including IAA23， IAA33， IAA41， and four GH3 genes were upregulated in type I callus. A total of 2968 transcription factors belonging to 56 transcription factor families in DEGs were significantly enriched. The expression levels of ZmEREB53， ZmEREB206， ZmEREB184， ZmLBD10， ZmLBD24， ZmLBD31， ZmLBD32， ZmWOX5b and ZmWOX9b， which are from AP2， WOX and LBD transcription factor families， were significantly up-regulated in type I callus. Genetic manipulation of these target genes may possibly promote the formation of maize callus. Collectively， this study identified candidate DEGs with great application potential， which provide theoretical reference for deciphering the molecular mechanism of maize callus formation.

Soybean is rich in nutrients and active substances. Oligosaccharides are beneficial functional components for human health， and identification of soybean oligosaccharide specific germplasms is of great practical significance. In this study， the content of oligosaccharides， including sucrose， raffinose， stachyose and total oligosaccharide， were quantified by high performance liquid chromatography （HPLC） method in a population of 264 soybean genotypes that were cultivated at two environments. It was shown that among the three oligosaccharides， sucrose accounts for the highest proportion of total oligosaccharides， and raffinose accounts for the lowest proportion. The total oligosaccharide content in Sanya city of Hainan province and Nanjing city of Jiangsu province ranged from 6.18%-11.46% and 4.19%-13.80%， respectively. Ten oligosaccharide specific genotypes with stable performance at different environments were obtained. Genome-wide association study（GWAS） was carried out by combining phenotypic and genotypic data of oligosaccharide content in natural soybean population. SNPs significantly associated with oligosaccharides content were identified and candidate genes were considered to be related with oligosaccharide/sucrose. Collectively， this study provided germplasm resources applicable for the selection and breeding of special soybean varieties， and also provided foundation for further exploring oligosaccharide candidate genes and developing their molecular markers in soybean.

Field weeds are the main factors affecting crop quality and yield，and chemical weed control is the primary strategy in modern agricultural production. Acetolactate synthase （ALS），also known as acetohydroxyacid synthase，is the critical enzyme in the biosynthesis of branched amino acids in plants. ALS inhibitor herbicides are also called ALS herbicides，which inhibit the biosynthesis of branched-chain amino acids in plants by disturbing the binding of ALS to substrates，achieving the purpose of killing weeds. With the widespread application of ALS herbicides in agricultural production，the problem of herbicide residues on subsequent crops is becoming more and more serious. The impact on crop yield and quality is particularly obvious. Thus，breeding for cultivars resistant to these herbicides would bring great advantage in effective weed control. Germplasm resistant to ALS herbicides has been created in a variety of crops through chemical mutagenesis and natural mutation，and resistant varieties have been successfully developed. In this study，the characteristics，types and scope of application for ALS herbicides，the resistance mechanism of ALS herbicide-resistant crops，and the research progress of germplasm creation and utilization of ALS herbicide-resistant crops were reviewed，which provides a better understanding of crops resistant to ALS herbicides. The innovation of herbicide germplasm and variety selection can provide reference，and make a simple prediction for the possible development of ALSresistant herbicide crops in the future.

This study reported the evaluation of the grain quality traits of rice landraces that were collected from "The Third National Action on Crop Germplasm Resources Survey and Collection" in 2017-2021. Out of 165 white and red grain samples， the highest variation coefficient was observed on the chalkiness of sticky rice， of which the coefficient of indica white grain was as high as 148.91%. The transparency was also detected with high variation coefficient， while the coefficient of brownish was below 5.0%. The variation coefficient of the waxy ratio amongst waxy rice was over 70.0%， the whiteness and the amylose content have lower value of the variation coefficient， while that of the three indexes including brownish， alkali elimination value and gel consistency were below 10.0%. In terms of the grain quality indexes that reaching the standard， the qualified ratio of whole grains or their chalkiness of sticky rice were below 50%， while the qualified ratio of other indexes were above 65.0%. For the glutinous rice， the qualified ratio of whole grains and the amylose content were around 50.0%， and that of the other indexes was higher than 65.0%. The cooking and edible quality is an important aspect in evaluating high-quality rice as well. Among all the varieties of sticky rice and glutinous rice， 60.7% and 35.4%， respectively， of which have reached or above the Grade 3 level issued by the Ministry of Agriculture and Rural Affairs on the three indexes of cooking and edible quality. Compared with the landraces that were collected during the second national survey in Fujian， the amount of landraces with high value of cooking and edible quality has increased significantly within the third survey. Through this study， 10 landraces were identified reaching the high-quality standard issued by the Ministry of Agriculture and Rural Affairs， which provide a material basis for the development of high-quality rice varieties in the future. In addition， 15 germplasms with high amylose content have been identified， which can be used as new raw materials for processing purpose specifically. The mid-colored rice were found with relatively rich amount in local landraces， accounting for about 20.0% of the total， they can provide valuable resources for future breeding and production of rice varieties with special function. In the future， works on the evaluation of grain quality for local landraces should be improved， and excellent germplasm resources would provide a material basis as well as promote the breeding of new superior rice varieties.

Anthocyanins and betalains are both natural water-soluble pigments with similar distribution and functions in plants. Anthocyanins is a type of phenylalanine-derived pigment， and the betalains is a tyrosine-derived pigment that contains the chromophore betalamic acid. Anthocyanins are widely distributed in plants， but in Caryophyllaceae plants， betalains present instead of anthocyanins. It is worth attention that anthocyanins and betalains have never been found in the same plant at the same time. This mutual exclusion may be raised by accident in evolution， or the co-existence of both pigments would bring troubles in plant survival. This article reviewed and discussed the general characters of anthocyanins and betalains， the biosynthetic pathways and their regulations， the cases of induced co-existence of the two pigments， and the possible reasons for the mutual exclusion between the two pigments. In addition， the possible mechanism of the mutual repulsion of the two pigments， the research focuses for the future， and the possible application value in molecular breeding are also prospected. The purpose of this article is to better understand the relationship between anthocyanins and betalains through the review and prospect of previous studies， reveal the mechanisms for their mutual exclusion， as well as provide new ideas and methods for research and application in related fields.