Agricultural germplasm resources mainly include crops， livestock and poultry， agricultural microorganisms and medicinal plants. There are 134，000 crop germplasm resources preserved in China， among which 74，000 are rice germplasm resources. How to accurately evaluate and utilize such huge rice germplasm resources is of great significance in rice germplasm innovation and breeding. In this paper， we reviewed the progress in collection， evaluation and accurate identification of rice germplasm resources， creation of new strains of rice， utilization of heterosis of rice， new techniques and methods of rice germplasm creation， and exploration and utilization of excellent genetic resources of rice， and summarized a new model of rice germplasm resource creation and utilization. Finally， this article discussed the current problems of rice core germplasm construction， germplasm resources identification and mining， and germplasm resources sharing and win-win mechanism. At the same time， we analyzed and prospeced how to strengthen the construction of specialized core seed resources， the accurate identification of germplasm resources， the innovative research of germplasm resources， the sharing mechanism of germplasm resources and the cooperation and exchange of germplasm resources， in order to provide some reference and help for further development of the identification， evaluation and innovative utilization of rice germplasm resources.

There are about 1000 species of Salvia L. in the world， and a large number of species have been developed in many aspects of medicine， ornamental， edible as well as essential oils and health care. In China， there are abundant germplasm resources including 84 species and 24 varieties， while only one native species， Salvia miltiorrhiza Bunge， is widely cultivated and used for medicinal purpose. It is urgent to investigate and classify their growth habits， morphological characteristics and horticultural characteristics， in order to promote their efficient development and popularization. Based on classical taxonomy and solid data obtained from field investigation and cultivation observation in the past 13 years， this article focuses on the classification and characterization of the horticultural characteristics of Salvia L.， including six aspects， such as end-use， flowering period， flower color， flower size， stem characteristics and garden shape. Spring-flowered （April-June）， summer-flowered （July-August）， and autumn-flowered （September-November） types were classified according to flowering periods， while purple， blue， red， pink， yellow and white flower styles were categorized based on flower colors. There were four levels for flower sizes： small （<1.5 cm）， medium （1.5-3.0 cm）， medium-large （3.0-4.5 cm）， and large （＞4.5 cm）， and three forms for stem types： trailing， cespitose， and erect. Notable flexibility were displayed in garden shape by a number of germplasms. The majority of domestic sage resources are the cespitose herbs with medium-sized （large） purple and blue flowers that bloom in the spring. This study improved the applied classification system of Salvia L.， and provided reference for further development and extensive application of Salvia L. species in China.

Forsythia suspensa （Thunb.） Vahl is a perennial woody plant with dimorphic style and self-incompatibility. Mining the candidate genes for the development of heterostyly in Forsythia suspensa is of significance to reveal its evolution and development mechanism. The whole genome resequencing of 40 long style morph and short style morph Forsythia suspensa plants were conducted， followed by the SNP variation sites detection in the sample genome and the selection sweep analysis. The candidate regions （top 5% level） were screened by the combination of genetic differentiation coefficient Fst and the nucleotide polymorphism π ratio. Differentially expressed genes （DEGs） were analyzed by GO and KEGG enrichment annotation， and qRT-PCR was used to detect the transcriptional profiles of candidate genes at different periods of style development. Selective sweep analysis showed that 295 genes were under selection， which mainly played an important role in plant growth and development and metabolic regulation. By analyzing the pathways in plant hormone regulation， the candidate genes CYP734A1 （EVM0010386）， BRI1 （EVM0011829） and CYCD3 （EVM0018316） of heterostyly in Forsythia suspense were analyzed. The gene expression results indicated that the CYP734A1 gene has sustained high expression during the critical period of development in F. suspensa's S-morph flowers. The BRI1 and CYCD3 genes in the S-morph were significantly higher than those in the L-morph during the crown exposure period. The candidate genes excavated in this study were differentially expressed in the L-morph and S-morph style of F. suspensa. Brassinosteroids was involved in the development of the heterostyly in F. suspensa， which was one of the reasons for heterostyly of F. suspensa. Collectively， this study laid a foundation for the evolution and genetic development regulation mechanism of heterostyly in F. suspensa.

This study identified a nuclear male sterile pepper line showing male sterility and parthenocarpy characteristics， and the agronomic traits and parthenocarpy fruit setting rate of the material were determined， and the parthenocarpy of the sterile line at different developmental stages and the parthenocarpy of the fertile line were determined for endogenous hormones. In addition， the genetic segregation of F₂ population was analyzed by field identification and microscopic examination， and the fertility segregation ratio of the population was analyzed by using the molecular markers developed by pepper nuclear male sterile genes ms-3， msw， ms， msms and msc-1.The results showed the vertical and horizontal diameters of the parthenocarpy fruit of the sterile line were larger， and the fruit setting rate of the fertile line and the sterile line was significantly different. The fruit setting rate of the fertile line was 22 %， and the fruit setting rate of the sterile line was 43%. The GA4 （gibberellin） content in the parthenocarpic fruit of the sterile line in different periods was significantly higher than that of the fertile line. There are 97 fertile lines and 30 sterile lines in the F2 population，with a separation ratio of 3.23 ： 1，which is consistent with the Mendelian inheritance law. It showsd that its fertility was controlled by a recessive single gene. We named the nuclear male sterile line of pepper as GMS702AB. This study provides a new nuclear male sterile line valuable in pepper breeding and seed production.

Dasypyrum villosum （L.） Candargy is one of wild relatives of common wheat （Triticum aestivum L.）. It is also the genetic resources for wheat improvement and has been widely used in recent years. In the long-term evolution process， D. villosum evolved many important agronomic traits， such as resistance to abiotic and biotic stresses， excellent quality， which are useful in wheat genetic improvement. This review paper briefly describes the homologous relationship between D. villosum and T. aestivum， the hybridization affinity with Triticum， and the effective methods for introgressing D. villosum chromosome disease，chromosome segments and genes into T. aestivum. The resistance genes of D. villosum to powdery mildew disease， sheath blight disease， stripe rust disease， eye spot disease， yellow mosaic disease， take-all disease， and cyst nematode disease on chromosome， the quality genes （such as ： high lysine content and polymorphic storage protein， etc.）， the drought tolerance， photoperiod and other genes were also reviewed. The application and great value of powdery mildew resistance genes of Pm21 and PmV in wheat improvement and breeding were introduced. The future research prospects and possible problems on D. villosum research were prospected.This review might provide reference for exploring and utilizing the beneficial genes of D. villosum， to broaden wheat genetic resources， accelerate the breeding process and functional investigation of important genes.

Lilium spp. are perennial bulbous herbs that include ornamental， edible， and medicinal lilies all with high economic value. Since Lily is identified with complex genetic background， high heterozygosity， incompatibility in distant hybridization， long cycle of traditional cross breeding as well as low breeding accuracy， it is difficult to breed for new varieties. These modern breeding techniques， including crossbreeding， mutation breeding， polyploidization breeding， protoplast fusion breeding， and genetic engineering， have been applied in lily. This article reviews and summarizes the methods for overcoming the barriers of distant hybridization in lily hybrid breeding， the treatments in mutation breeding， the technical approaches in polyploidization breeding and haploid breeding， the methods in protoplast isolation and fusion in protoplast fusion breeding， and the advances in lily genetic transformation and editing. In addition， several examples in lily breeding methods and techniques are listed， and the problems in techniques are proposed. The future development and application prospects in lily breeding technologies are discussed. This article aims to provide insights for future research on lily breeding technologies and their applications in breeding of lily varieties.

A two-year field trials and agronomic performance analysis were conducted in 179 foxtail millet accessions which are homonyms of the four famous landraces （Qinzhouhuang， Taohuami， Longshanxiaomi and Jinmi），as well as exploring the genetic relationships between the materials. Fifty-one accessions including the four original varieties were subjected for nutritional quality analysis. The significant differences on quantitative traits were detected in 179 accessions， while the quality traits were detected without much differences. Through the correlation analysis， a highly significant positive correlation between kernel weight of single ear and ear weight， as well as stalk weight and ear weight， were found. Principal component analysis showed that the first three principal components contributed to 89.414% of the cumulative variation. Gained from the composite score F-value calculated based on the results of principal component analysis， we observed the highest and lowest composite scores in Bocaigen-30 （F=7.42） and Yintianhan-27 （F=-9.17）， respectively. Cluster analysis based on agronomic traits revealed that the 179 accessions were classified into four clusters with relatively concentrated clustering within homonyms varieties and greater similarity of agronomic traits. The results of phylogenetic relationships indicate that there are diverse evolutionary relationships among different varieties with the same name， and there may be gene exchange between different varieties with different names. The differences in nutritional quality among the four categories of famous foxtail millet were small， and the differences in all nutritional quality indexes except crude fiber were not significant. The crude protein and crude fat was positively correlated， while starch negatively correlated with protein and fat. The first six major components explained 88.414% of the cumulative variation and could basically cover the information of the 10 nutritional qualities. Based on the principal component analysis of nutritional qualities， the top ranked varieties were Bocaigen-77， Bocaigen-33， Bocaigen-44， Papocao-15， Jugenqi-25 and Yintianhan-13， combined with the comprehensive scores of different accessions.This study identifies and studies the agronomic traits， quality traits， and phylogenetic relationships of the four famous foxtail millet groups， which is of great significance for the protection and utilization of germplasm resources， as well as the excavation of excellent resources.

Grafting is beneficial in enhancing the adaptability to biotic and abiotic stresses， and improving the yield and quality of grapes. There are varieties of grape rootstocks， while their precise clarification become complex and difficult. Deep learning， capable of rapidly capturing deep features from images， has been extensively applied in the field of plant image classification and recognition. In this study， the mature leaf images of 30 grape rootstocks were deployed to construct a dataset， comprising 13547 grape rootstock leaf images. Four convolutional neural networks， GoogleNet， ResNet-50， ResNet-101 and VGG-16， were used for image recognition. The results show that the classification network with the highest accuracy is ResNet-101， and the accuracy reaches 97.5 % under the optimal model parameters （learning rate：0.005， mini-batch size：32， Max epochs：50）. Among the 30 varieties， the average prediction precision rate was 92.59%， and the prediction precision reaching 100% was observed in seven varieties. The recall rate of eight varieties reached 100%， and the average recall rate was 91.08 %. The leaf surface texture， leaf vein and leaf margin were major factors that influence variety classification. This study confirmed the application capacity of deep learning network models in real-time automatic identification of grape rootstocks， thus providing reference for the protection， utilization， clasification research of grape rootstock varieties and the variety recognition of other crops.

The salt-alkali tolerance of 286 brassica rapeseed strains at the seedling stage was identified in this study. The indexes of leaves number， green leaves number， green leaves rate，shoot length， root length， and root weight under salt-alkali stress were determined through a hydroponics test. Various methods including comprehensive assessment value （D-value）， extreme material screening analysis， correlation analysis， principal component analysis， membership function analysis， frequency analysis and stepwise regression analysis were used. A comprehensive evaluation model for saline-alkali tolerance was established for different genotypes of rapeseed germplasm at the seedling stage and appropriate evaluation indexes were selected. Under saline-alkali stress conditions， leaves number showed a negative correlation with plant height but the correlation coefficient between them was not significant； however other traits showed positive correlations that reached a significant or extremely significant level. Principal component analysis was employed to synthesize 7 salt-alkali tolerance indexes into 4 principal components which could represent 88.349% of the original data information on rape′s salt-alkali tolerance. Based on the relative importance （weight） of these four principal components， a comprehensive evaluation value （D-value） for salt and alkali tolerance among different genotypes was obtained. Four alkali-salt-tolerant brassica napus germplasms and four saline-sensitive germplasms were screened using membership function analysis and extreme material screening analysis respectively. The results from stepwise regression analysis indicated that salinity tolerance in brassica napus germplasm resources could be determined by measuring the green leaves number， green leaves rate， shoot weight as well as root length and root weight at the seedling stage while estimating D-value using regression equations.

Based on "The Third National Campaign of Crop Germplasm Census and Collection"， genetic diversity analysis， correlation analysis， cluster analysis and principal component analysis were used to study and evaluate 136 sorghum germplasm resources collected from 2020 to 2021 in Hebei province. The results showed that there were abundant phenotypic variations in the collection. The diversity index of 15 phenotypic traits ranged from 0.0844 to 1.9926， and the coefficient of variation ranged from 4.69% to 68.00%. The genetic diversity of 1000-grain weight and the coefficient of variation of panicle shape were the highest. A significant positive correlation between plant height and panicle traits was detected. These germplasms were divided into three groups by cluster analysis， while three clusters didn’t correlate with the geographic collection sites. Cluster I showed the best performance in panicle traits and could be used in breeding for technical purposes. The plant height of cluster II was low， which can be used as dwarf resources in germplasm innovation. Cluster III showed the best yield traits which can be used in breeding for higher grain production. Principal component analysis simplified phenotypic trait factors into 5 principal components， with the cumulative contribution rate of 60.182%. The plant height， the panicle and grain traits were the main factors contributing to the phenotypic differences. Based on the comprehensive scores of 136 sorghum germplasms that ranged from 0.107 to 1.147， the top 10 elite germplasm resources such as Suning sorghum， Changsui sorghum， Tiaozhou sorghum and Luoshu were selected. Collectively， based on the evaluation of these newly collected sorghum germplasm resources in Hebei province， this study provided insights for the mining of elite sorghum germplasm and the innovative utilization of germplasm.

Chlorophyll is an important pigment in rice photosynthesis， and its synthesis determines the efficiency of photosynthesis， ultimately affecting plant yield and quality. In this study， it was found that the glycogen synthase kinase OsGSK2 overexpressed Go-2 plants exhibited a dark green leaf phenotype at heading date. Compared to the wild type， the contents of chlorophyll a， chlorophyll b， and carotenoids in Go-2 plants significantly increased. The observation results of transmission electron microscopy showed that compared to the wild type， the chloroplast thylakoid layers of Go-2 plants increased. Yeast two hybrid "one-to-one" experiment confirmed the interaction between OsGSK2 and the Golden2-Like transcription factor OsGLK1， and further confirmed the interaction between OsGSK2 and OsGLK1 through bimolecular fluorescence complementarity experiments. By detecting dual luciferase activity in rice protoplasts， it was found that compared to single transgenic OsGLK1， co transfection of OsGSK2 and OsGLK1 significantly increased the expression level of downstream target genes. The fluorescence quantitative PCR results showed that compared to the wild-type， the transcription levels of target genes （OsPORB， OsCAO1， LHCB6， etc） directly regulated by OsGLK1 were significantly up-regulated in Go-2 plants. These results provided preliminarily insights on the molecular mechanism of the interaction between OsGSK2 and OsGLK1 in regulating rice chlorophyll synthesis and chloroplast development， extending the molecular function of rice glycogen synthase kinase， enriching the regulation network of rice leaf color， and providing theoretical basis for high photosynthetic molecular breeding in rice.

Evaluating the genetic relationship of sweetpotato varieties is an important prerequisite for understanding their genetic background and effectively utilizing germplasm resources. Using 23 pairs of InDel primers developed by our research group， genotype analysis was conducted on 305 registered sweetpotato varieties in China. A total of 56 bands were amplified， of which 53 bands were polymorphic， with a polymorphism rate of 94.6%. The average values of polymorphic information content （PIC）， Nei's genetic diversity index （H）， observed heterozygosity （Ho）， and expected heterozygosity （He） are 0.4098， 0.4451， 0.6003 and 0.4460， respectively. Group structure analysis indicates that ΔK reaches maximum at the number of groups K=2， with a small peak at K=4. The northern and Yangtze River sweetpotato zone are evenly distributed within the two groups， while the majority （72.97%） of the southern sweetpotato zone converge in group 2. The principal coordinate analysis （PCoA） shows that there is some convergence in the southern sweetpotato zone， but there is no clear cluster division overall. The clustering results divided the population into four main groups. The varieties in the northern sweetpotato zone and the Yangtze River sweetpotato zone were evenly distributed in groups I， II， III and IV， while the southern sweetpotato zone （77.03%） was mainly concentrated in group IV. This clustering result is basically consistent with population structure research and principal coordinate analysis. Genealogy analysis identified the main parental materials of 13 registered varieties， and there was a situation of repeated use of parents for forward and reverse breeding in each breeding unit. This article combines molecular marker results with pedigree information， preliminarily indicating that the registered varieties of sweetpotatoes in China have close genetic relationships and narrow genetic backgrounds， providing reference for germplasm innovation and new variety selection of sweetpotatoes.

There are 33 wild orchids listed in the national first-class plant protection catalog of China， belonging to several genera including Paphiopedilum Pfitzer， Cypripedium L.， Cymbidium Sw.， Dendrobium Sw.， Phalaenopsis Blume， and Calanthe R. Br.. These species have important ornamental and medicinal value. The registration status of hybrids， their parents selection， pollination and sowing time selection， intergeneric hybridization progress and problems in hybrid breeding of these species have been reviewed. Based on the current breeding situation future breeding directions are proposed. To date， 3611 hybrids with the national first-class wild orchids as parents have been registered in Royal Horticultural Society. The top ten species of hybrids are Paph. spp.， including Paph. bellatulum （Rcnb. F.） Stein.， Paph. insigne （Lindl.） Pfitz. and Paph. spicerianum （H. G. Reichenbach） Pfitzer.， followed by Cymb. Insigne Rolfe. However， no hybrid has been registered with Paph. erythroanthum Z. J. Liu，X. Y. Liao & S. R. Lan， Paph. guangdongense Z. J. Liu & L. J. Chen， Paph. notatisepalum Z. J. Liu，M. Wang & S. R. Lan，and Cypr. subtropicum S. C. Chen & K. Y. Lang as parents.There are few hybrids with Paph. hangianum Perner & O. Gruss， Dend. flexicaule Z. H. Tsi， Dend. huoshanense C. Z. Tang et S. J. Cheng， Cymb. wenshanense Y. S. Wu et F. Y. Liuas parents， although these species are all excellent with potential breeding value. To improve the utilization of wild orchid germplasm resources in future breeding， we propose to make full use of these wild germplasm resources in intergeneric hybridization， in combination with molecular marker assisted selection technology. This article can provide reference for the hybrid breeding of the national first-class wild orchids， and provide support for the innovation of orchid germplasm resources and the sustainable development of the orchid industry.

In order to strengthen the protection and utilization of Cymbidium resources in China， ISSR markers were deployed to conduct the genetic diversity and fingerprint construction in this study. A total of 67 bands were detected with 11 primers in 96 samples， and the average of polymorphic bands rate is 73.63%， the number of alleles （N_a） is 1.925， the number of effective alleles （N_e） is 1.450， the genetic diversity of Nei's is 0.277， and the Shannon index （I） is 0.427， the percentage of polymorphic loci （PPL） is 92.54%， the genetic diversity within populations （H_s） is 0.1934， the genetic differentiation coefficient （G_st） is 0.3009， the total genetic diversity （H_t） was 0.2767， and the average gene flow among populations（N_m） of Cymbidium is 1.1619， the fixation index of pairwise genetic differentiation among populations ranged from 0.002 to 0.527， with an average of 0.325. The systematic clustering results indicate that Cymbidium has high degree of genetic differentiation， which was divided into three groups by systematic clustering analysis， Cymbidium. goeringii and C. sinense were assigned to one category， while C. kanran， C. goeringii var. longibracteatum， C. faberi， C. tortisepalum and C. ensifolium were in the second category， the hybrids were in a unique category， which has a large genetic distance from the other two categories. Principal coordinate analysis indicated that C. tortisepalum and C. goeringii showed a distant genetic relationship. 6 pairs of primers were selected to construct fingerprint QR codes for 96 species. Collectively， this study provided an important basis for the breeding and variety identification of Cymbidium in the future.

The bHLH transcription factor families is one of the largest families of transcription factors in plants. More and more plant bHLH transcription factor families have been identified presently. However， systematic analysis of the lily bHLH transcription factor family has not been reported.Based on the transcriptome data of Lilium， this study identified a total of 74 bHLH family members. All the bHLH proteins were hydrophilic， 93% of which were unstable proteins， and 61% were acidic proteins.Through the domain analysis， 25 conserved residues were accounted for ≥50% consistence， with R16， R17， L27， L49 and L59 being highly conserved. Sixty-four bHLH proteins were predicted to bind to DNA， including 58 E-box and 46 G-box bindings. Based on the phylogenetic analysis， lily bHLHs were assigned into 21 subfamilies， and they were annotated with functions on signal transduction， abiotic stress， plant growth and development， and substance synthesis. Three genes Unigene23213_All， CL1682.Contig2_All and CL8286.Contig2_All， which associated with ocimene and linalool biosynthesis， were cloned showing 97.30%-99.89% homology among varieties at each locus. The three genes were expressed in flowers， leaves and scales. The fundings of this research provided a valuable information in further study deciphering the functions of lily bHLH transcription factors.

Tropical crops， including banana， papaya， sugarcane， cassava， rubber tree， oil palm， etc， are of importance in Chinese agriculture， which not only provide raw materials for our daily life and industrial and agricultural production， but also contribute to the main agricultural output and economic growth in tropical and subtropical zones of China. There are many barriers in tropical crops in use of modern molecular breeding techniques， such as polyploidy， heterozygous， vegetative propagation， long juvenile phase and large size of plants， etc. The genetic improvement of tropical crops through conventional breeding is troublesome， time-consuming， low efficiency and less progress. The development of genome editing technologies has brought a new way in tropical crops breeding. CRISPR-Cas9 mediated genome editing has been widely used in plants， profited from its higher targeting efficiency， versatility and ease of usage. This approach has been applied in banana， cassava， rubber tree， and sugarcane. Here， we focus on the recent advances based on CRISPR/Cas9 methodologies， and summarize their application in tropical crops breeding， as well as propose future perspectives and challenges in improving tropical plants.

Cistanche salsa （C. A. Mey.） G. Beck is a parasitic herb of the genus Cistanche in the family Orobanchaceae， which has the largest number of host plant species identified. It is a traditional Chinese medicine commonly used in northwest China. To clarify the distribution of C. salsa resources and the identify host plants， 66 samples of C. salsa with parasitic roots were analyzed using DNA barcoding and morphological characters. The results indicated that C. salsa was widely distributed in regions of Xinjiang and Ningxia municipality， at the elevation ranging from 400 to 2000 meters. Specifically， the host plants in Yumin County， Tacheng City， Xinjiang Municipality including Seriphidium compactum （Fisch. ex DC.） Poljakov（Seriphidium genus， Asteraceae） and Atriplex verrucifera Marsch. von Bieb. （Atriplex genus， Amaranthaceae）， the host plants in Tacheng County of Tacheng City including Kalidium cuspidatum Ung.-Sternb. （Kalidium genus， Amaranthaceae）， A. verrucifera and Krascheninnikovia ceratoides （L.） Gueldenst. （Krascheninnikovia genus， Amaranthaceae）， among which A. verrucifera was the dominanthost. The host plants in Habahe County， Altay City， Xinjiang Municipality including S. compactum， Krascheninnikovia ewersmanniana （Stschegl. ex Losinsk.） Grubov （Krascheninnikovia genus， Amaranthaceae） and K. ceratoides， the host plants in Jimunai County of Altay City including K. ceratoides and S. compactum. In Yiwu County， Hami City， Xinjiang Municipality， Iljinia regelii （Bunge） Korovin （Iljinia genus， Amaranthaceae） served as the dominant host plant， and the host plants in Barkol County of Hami City including K. cuspidatum. Besides， Suaeda physophora Pall （Suaeda genus， Amaranthaceae） washost plant in the plantation of Jumisar of Changji City in Xinjiang Municipality. Kalidium gracile Fenzl（Kalidium genus， Amaranthaceae） and Sympegma regelii Bunge （Sympegma genus， Amaranthaceae） were both host plants in Yanchi County of Ningxia Wuzhong region， with S. regelii being the dominanthost. And S. compactum is a new recorded host plantof C. salsa. Collectively， this study provided scientific basis for protection and breeding of C. salsa and its cultivated host plants in future.

Ratooning ability is a key trait in sugarcane breeding. But the high effective evaluation of ratooning ability is still a difficulty because of the long growth period of sugarcane. This study involved the utilization of 212 sugarcane germplasm resources gathered from diverse countries and regions. A rapid-cycled evaluation of sugarcane ratooning ability was performed in Longan city of Guangxi Zhuang Autonomous Region and Kaiyuan city of Yunnan province， respectively. The assessment focused on the emergence rate of seedlings and aboveground stalk biomass during two seasons. The ratooning ability of sugarcane is greatly affected by environmental conditions， and extremely significant differences were found among four ratooning indexes at two field sites， and highly significant positive correlation were detected among theses indexes. Principal component analysis shows that only one principal component was extracted for both experimental sites， whose contribution rate reach to 80.25% in Kaiyuan site and 62.68% in Longan site. By using cluster analysis method， 212 germplasm resources were divided into 5 clusters. Cluster I contained 9 materials， each index for ratooning ability of them performed the highest level， belonging to the strongest ratooning ability germplasm resources. There were 38 materials belonged to cluster II and cluster III with higher level performance in indexes of ratooning ability. They could be as stronger ratooning germplasm resources to be used in future breeding plans. The rest 165 materials grouped into cluster IV and cluster V as weak ratooning ability clones. Based on above analyses， it is of great significance for the sustainable development of the sugarcane industry to utilize the elite materials to improve the ratooning ability of varieties.

In order to explore the genetic mechanism of wheat seminal root formation and growth， 198 wheat natural cultivars growing in the Huang-Huai Area were investigated in this study. The total first grade branch number， branch density， length， surface area， volume and average diameter of the radicle were examined using 21 days old seedlings in the hydroponic culture. Q+K mixed linear model， in conjugation with the genotyping results by the 660K gene chip， was used to conduct genome-wide association analysis （GWAS） at radicle traits， followed by functional annotation and candidate gene mining of significant and repeatedly-detected association sites. At the six radicle growth traits， a continuous and normal or nearly normal distribution were observed， with coefficient of variation of 5.56%-22.10%. A total of 136 significant association sites were detected. They were distributed on the chromosomes except 7B， each of which could explain 5.10%-13.60% of phenotypic variation. Thirteen significant pleiotropic sites were identified from those 136 sites. Based on the annotation， 16 candidate genes that may be related to radicle growth were found， such as TraesCS4A01G023100， TraesCS1B01G294400， TraesCS4A01G006200. These genes were proposed to be involved in the formation of wheat radicle root system by regulating DNA topoisomerase， ubiquitin-conjugating enzyme E2， phosphoinositide phosphatase family protein and so on. The results of this study provided a reference for the construction of wheat root regulation network， as well as the optimization of root architecture and function.

To investigate the floral aroma metabolism pathways and differentially expressed genes （DEGs） involved in the biosynthesis of terpenoid aroma compounds in tropical waterlily organs， the transcriptome sequencing was used to analyze the flower organs including the petal （PE）， stamen （ST） and pistil （PI） of N. ‘Paul Stetson’. The number of differentially expressed genes in PE-vs-PI， ST-vs-PI， and ST-vs-PE were 7853， 7501， and 2526， respectively. GO classification and enrichment analysis showed that these DEGs were mainly involved in biological regulation， cellular processes， metabolic processes， and stimulus response biological processes. KEGG annotation revealed abundant pathways with significantly enriched DEGs in PE-vs-PI， followed by ST-vs-PI， and ST-vs-PE. Ninety-eight of 794 DEGs that shared in three comparative groups were enriched in four terpenoid floral aroma synthesis pathways， and DEGs in PE-vs-PI and ST-vs-PI was higher than that in ST-vs-PE. In petals and stamens， the genes responsible for synthesis of acacia aldehyde and diterpenoid kaurene， were expressed at higher levels than in pistils. Six of 98 DEGs were randomly selected and subjected for qRT-PCR analysis， confirming the trend on transcriptional expression as revealed by transcriptome sequencing. The results provided a scientific reference for future deciphering the molecular mechanism of terpenoid aroma compounds biosynthesis in tropical waterlilies.

Glycosylation modification plays a crucial role in the growth and development of plants. The core enzyme that catalyzes the synthesis of glycosylation products is glycosyltransferase （GT）， which includes the UGT family members. The UGT family primarily utilizes uridine diphosphate （UDP） as the glycosyl donor. Plant glycosylation reactions play a crucial role in regulating the solubility， stability and bioactivity of various plant metabolites. Furthermore， they are closely associated with plant quality traits， the storage of volatile compounds and respond to abiotic and biotic stresses. This study reviews the structural and catalytic features， reaction types， and functional classifications of glycosyltransferases in ornamental plants. It summarizes current research progress on modifications of hormones， terpenoids， and flavonoids in ornamental plants via UDP-glycosyltransferases， eventually affecting flower color， leaf color， leaf morphology， plant adversity， and functional compounds. By reviewing and summarizing the relevant studies， this study will help to understand the role of glycosyltransferases in the metabolic regulation of ornamental plants. Furthermore， this will also lay a foundation for future innovation in ornamental plant germplasm improvement and the development of functional components.

The genetic diversity， correlation， principal component analysis， clustering and comprehensive evaluation were employed to analyze and assess agronomic traits of 296 peanut germplasm resources. The results revealed that the genetic diversity index for three quality traits ranged from 0.526-0.909， while the genetic diversity index for ten quantitative traits varied between 0.834-2.007. Additionally， the coefficient of variation ranged from 3.268%-68.198%. These findings indicated that these peanut germplasm resources possess abundant genetic information. Correlation analysis suggested significant associations between emergence uniformity， whole growth period， leaf shape， productivity per plant and yield. Principal component analysis extracted six principal components with a cumulative contribution rate of 78.336%， representing most of the agronomic traits. Cluster analysis divided this collection into two categories including four groups based on their characteristics related to high-yield potential， small-grain size， early-maturity or large-grain size. These groups can serve as candidate materials for future germplasm selection in breeding programs. A comprehensive score was constructed using fuzzy membership function based on contribution weights assigned to six principal components， which was shown as F=0.323F₁+0.257F₂+0.122F₃+0.108F₄+0.010F₅+0.091F₆. Four peanut germplasm resources displaying favorable comprehensive traits were selected as potential candidates for future peanut breeding. This study offering a theoretical basis for selecting appropriate parental lines and specific in future breeding programs.

WRKY transcription factor is one of the most abundant transcription factors in higher plants， and play an important role in plant growth and development， senescence， abiotic and biological stress. O. granulata （Oryza meyeriana subsp. Granulata）， which serves as a closely related wild species of cultivated rice， represents the characteristics of shade tolerance， drought tolerance and high resistance to bacterial blight， becoming an important germplasm resources for cultivated rice improvement. In this study， using HMMER， Pfam， SMART， TBtools， NCBI software and website， 94 genes encoding WRKY transcription factors （OgWRKYs） were identified in O. granulata， which were unevenly distributed on 12 chromosomes. According to the number of WRKY domains and the characteristics of zinc finger structure， they were divided into groups Ⅰ， Ⅱ， Ⅲ and Ⅳ. Group II had the most members （52）， which was similar to other species. In addition to the conserved WRKYGQK heptaeptides， six variants were identified， among which WRKYGHK， WRRYGQK， WRKYAKK and WRKYSQK were new variants reported for the first time in plants. According to conserved domain analysis， OgWRKY61， OgWRKY71 and OgWRKY77a may be associated with plant disease resistance.KEGG pathway enrichment analysis showed that 14 OgWRKY transcription factors were enriched in plant-pathogen interaction pathways， and 10 of them were simultaneously enriched in MAPK signaling pathways. Further analysis of cis-acting elements suggested that OgWRKY30b， OgWRKY53， OgWRKY88， OgWRKY96 and OgWRKY111 might play important roles in response to biological and abiotic stresses in O. granulata. The results of qRT-PCR analysis showed that the expression of OgWRKY30b， OgWRKY53， OgWRKY88 and OgWRKY111 genes were induced by PXO99， while the expression of OgWRKY96 was inhibited by infection. The results of this study provided important reference for future mining of OgWRKYs genes in O. granulata.

Understanding plant responses to pathogen invasion is important for identifying resistance genes. In this experiment， the seedlings of wheat variety Zhongzuo 9504 were inoculated by powdery mildew， in order to observe the changes of growth， physiological metabolism-related indexes and gene expression in wheat leaves at 0 h， 6 h， 1 d， 4 d and 7 d after infestation. This study attempted to reveal the response mechanism of powdery mildew inoculation on the growth， osmoregulatory substances and reactive oxygen species in wheat. The results showed that with the increase of inoculation time， the number of dead cells， and the activity of peroxidase and superoxide anion in wheat leaves tended to increase. The powdery mildew produced mature secondary conidia 7 days post inoculation. Phenylalanine ammonia-lyase and polyphenol oxidase functioned at different infestation times. Soluble protein content basically showed an increasing trend， chlorophyll content decreased significantly at 7 d， which in turn affected plant growth. No significant changes on hydrogen peroxide content was observed throughout the inoculation period. Transcriptome analysis revealed that PTI signaling was partially inhibited and down-regulated at the initial stage of inoculation （0 h to 6 h）. PTI signaling responded positively in the early and middle stages （6 h to1 d and 1 d to 4 d）， while ETI appeared in the early stage. In the late stage of infestation （4 d to 7 d）， there was a down-regulation tendency on both the PTI and ETI signaling pathways， as powdery mildew had colonized the surface of the leaf completely， thus compromising photosynthesis in wheat.

During the Third National Crop Germplasm Resource Survey and Collection Action in Fujian province from 2017 to 2022， total 6235 accessions of crop germplasm resources including local crop varieties and their wild relatives were collected. The numbers of vegetables， food grains， fruit trees， economic crops， and forage and green manure crops collected in this action accounted for 33.57%， 25.91%， 24.35%， 14.59%， and 1.57% respectively， and they belong to 83 families， 229 genera， and 313 species. Among these gerplasm resources， 16.04% belong to the Leguminosae faminly with the highest persentage. Different regions and altitudes were relevant to the species types and quantities of crop germplasm resources collected. Among the 9 prefecture level cities in the province， Sanming city had the highest number of germplasm resources， accounting for 20.65%. The number of crop germplasm resources decreased along with the increase of altitude. The number of collections at different altitude was correlated with the number of counties （cities， districts）， townships（towns）， and villages（streets）. The Shannon-Wiener diversity index and Brillouin richness index were the highest at an altitude of 0-200 meters in the six prefecture level cities （Zhangzhou， Fuzhou， Ningde， Quanzhou， Putian， Xiamen） in coastal areas， while these two indexes are relatively high at the altitudes of 200-400 meters， 400-600 meters， and 600-800 meters in three mountainous areas， such as Longyan， Sanming， and Nanping， respectively. Through the correlation analyses of species diversity index in different prefecture level cities and altitude intervals， a highly significant positive correlation was detected between the Shannon-Wiener diversity index and Sanming and Longyan cities， while a highly significant negative correlation was detected between the Pielou evenness index and Putian and Longyan cities， which might be closely correlated to the agricultural economic zones in the province. Thus， by investigating the diversity of crop germplasm resources in Fujian province， this study provided reference and scientific basis for the development， utilization， and excavation of local crop germplasm resources to meet local demands.

Lilium spp. originates in China and holds an important and special position in China's flower industry. Floral fragrance is a important label of its ornamental traits. Previous studies have shown that the main floral components of different fragrance types vary greatly. The differences between strongly scented lilies and lightly scented lilies are mainly concentrated in the differences of terpenoids， while the sampling period， location， environment， hormones and so on might lead to changes in floral components. At present， the research on the synthetic pathway of lily fragrance substances focuses on the terpene synthesis pathway， mostly on the functional study of terpene synthase and the analysis of the upstream regulatory network， while the functional analysis of the genes of the metabolic pathway of other floral fragrance components and the research on the molecular regulatory mechanism are still a lot of unanswered questions. Due to the lack of comprehensive and in-depth analysis of synthesis and regulatory mechanisms in floral fragrance， it is difficult to support precise modification of floral fragrance， ultimately resulting in a slow process of floral fragrance breeding. In-depth exploration and utilization of lily floral fragrance genes and improvement of related metabolic pathways and regulatory networks may be the following focus of lily floral fragrance. In this paper， we review and summarize the previous research on lily floral fragrance， and prospect the future research direction， in order to provide a reference for the subsequent research on the molecular regulatory mechanism of lily floral fragrance， and provide a reference for the directional cultivation of new lily varieties with pleasant fragrance.

The diverse array of variegated leaf plant species is characterized by a rich tapestry of chromatic patterns adorning their foliage. These distinctive markings not only confer aesthetic significance as a pivotal ornamental trait in plants， but also bear ecological and biological relevance such as facilitating reproductive processes， acting as a deterrent against natural adversaries， and adaptability to environmental fluctuations. This article provides a comprehensive review of recent advancements in the classification and formation of leaf variegation in ornamental plants. Based on distinctions in pigment accumulation and structural characteristics within leaf tissues， the microstructural categorization of variegation types is outlined， and the molecular mechanisms underlying leaf variegation formation are summarized. Alterations in structural genes and transcription factors that associate with pigment synthesis and metabolism， such as CHLH， DFR， CRD1， during leaf development， as well as mutations in genes that are related to organelle development and cell differentiation， including ZAT10 and VAR3， can directly or indirectly participate in leaf variegation formation by influencing differential pigment accumulation and altering leaf structure. While there have been numerous studies on the formation mechanisms of leaf variegation in ornamental plants， the genetic mechanisms underlying leaf variegation remain unclear. The reasons for differential gene expression in variegated leaf regions and the spatial distribution mechanisms of leaf variegation patterns are still areas requiring further investigation. Future research can involve the selection of model plants from variegated leaf species， building the pan-genome， integrating with multi-omics technologies such as pan-genomics， proteomics， and metabolomics. This approach might enable exploring the origin of leaf variegation， uncovering the adaptive responses of variegated leaf plants to their environments， and deciphering the evolutionary mechanisms underlying important plant traits and environmental adaptation.

Camellia oleifera Abe. is an important woody oil tree in China， which has important economic and social benefits. Cryptochrome is one of the blue light receptors in plants， which is involved in the growth and development of plants such as flowering regulation and photomorphogenesis. In this study， CoCRY1 gene was cloned from C.oleifera 'Huashuo'. Bioinformatics analysis showed that CDS sequence length of CoCRY1 was 2262 bp encoding for 684 amino acids， the molecular formula of the CoCRY1 protein was C₃₄₅₄H₅₂₈₁N₉₇₁O₁₀₂₇S₁₈， and the molecular weight was 77.42 kDa， further analysis showed that this protein sequence contained three domains， namely DNA_photolyase， FAD_binding_7 and Cryptochrome_C， which proved that CoCRY1 protein belongs to cryptochrome family. Homologous sequence and phylogenetic tree analysis showed that CoCRY1 protein had the highest similarity with the homologous sequence of tea tree （Camellia sinensis）. Tissue expression analysis showed that the transcripts of CoCRY1 were the highest in stem and the lowest in flower. Arabidopsis thaliana plants with heterogenous expression of CoCRY1 gene were obtained by Agrobacterium transformation， and transgenic plants were identified from DNA and RNA level. Phenotypic analysis of transgenic Arabidopsis showed that overexpression of CoCRY1 gene induced early flowering under long day londition and had photospectific inhibitory effect on hypocotyl elongation. Through bioinformatics， quantitative analysis and heterologous expression， it was found that CoCRY1 gene plays an important role in the biological process of C.oleifera flowering.

Cineraria （Pericallis hybrida） is an ornamental plant with multiple anthocyanin metabolic pathways and has varieties showing rare flower color phenotypes such as blue and bicolor. Elucidating the molecular mechanisms underlying the formation of these flower colors can provide valuable genetic resources in breeding of ornamental plants， particularly for the development of new blue flower varieties. Based on research experiences on cineraria， the authors summarized the research progress in the past 20 years on the unique anthocyanin structure， the regulatory pathways of anthocyanin biosynthesis， and the technical approaches used in flower color research. This review mainly introduces： （1） The pigment composition of different cineraria varieties and the polyacylation structure in blue varieties； （2） The genes involved in anthocyanin metabolism pathways such as polyacylation and acylation， and the function of transcription factors such as MYB and MADS-box in regulating flower color and spot formation； （3） The efficient genetic transformation system and viral-induced gene silencing system relevant to flower color research in cineraria， as well as progress in these areas. This article would like to provide references for future research on the flower color and molecular breeding of cineraria and other ornamental plants.

Flower fragrance is a significant ornamental feature of ornamental plants. As a worldwide woody flower， Rhododendron is famous because of rich colors and diverse patterns. Its floral fragrance is an important indicator of its quality. Studies have demonstrated that terpenoids， alcohols， esters， and ketones are the main components of rhododendron florets， and the release of these compounds is influenced by flower development， release site， and environmental conditions. Terpenoids are the most important floral components of Rhododendron， and terpenoid synthase gene is the main regulator in the metabolic pathway of floral substances. Through the genomics and metabolomics study， TPS family genes of Rhododendron ovatum were much more than those of other odorless Rhododendron plants. It is of great significance to study the floral composition and biosynthesis pathway in different species and varieties of Rhododendron for aromatic breeding and comprehensive utilization. This study reviewed the determination and analysis methods of floral composition of Rhododendron plants， the aroma components of Rhododendron species in subgenera， the synthesis and release law of aroma， and the biosynthetic pathway of aroma and key genes， which provided reference for the study of the genetic law of the synthesis and metabolism of main floral substances and breeding new varieties with obvious characteristic fragrance in Rhododendron.