Preharvest Sprouting Resistance Test and Functional Markers Assisted Genotyping in Wheat Germplasm Resources

GONG Chengru,YUAN Yuhao,LIU Zhen,ZHENG Jizhou,TIAN Zhicheng,LIU Shengli,SHEN Qinghua,HUANG Zhenpu,DONG Chunhao,GAO Yan,LI Qiaoyun,TANG Jianwei,JIAO Zhuqing,YIN Guihong

PDF(973 KB)
PDF(973 KB)
Journal of Plant Genetic Resources ›› 2024, Vol. 25 ›› Issue (9) : 1493-1503. DOI: 10.13430/j.cnki.jpgr.20231113002
Research Articles

Preharvest Sprouting Resistance Test and Functional Markers Assisted Genotyping in Wheat Germplasm Resources

  • GONG Chengru1, YUAN Yuhao1, LIU Zhen1, ZHENG Jizhou2, TIAN Zhicheng1, LIU Shengli1, SHEN Qinghua1, HUANG Zhenpu1, DONG Chunhao1, GAO Yan1, LI Qiaoyun1, TANG Jianwei1, JIAO Zhuqing3, YIN Guihong1
Author information +
History +

Abstract

This study aims to identify the pre-harvest sprouting resistance in wheat germplasm resources and molecular markers for marker-assisted selection, ultimately obtaining excellent white grain wheat germplasm resources applicable in wheat breeding. These spikes of 222 wheat germplasm resources were tested for pre-harvest sprouting resistance, and the functional markers of eight anti-pre-harvest sprouting genes, including myb10DDFR-BVp1B3PM19-A1MFT-3AMFT-A2MKK3-AQSD1, were used for genotyping. Phenotypic identification results showed that there were significant differences in the relative sprouting percentage of 222 wheat germplasm resources. The relative sprouting percentage ranged from 0 to 1.15, and the average relative sprouting percentage was 0.73. 38 wheat materials were identified to be resistant to pre-harvest sprouting, including 9 white wheat, 27 red wheat and 2 black wheat. Correlation analysis between allele types and relative sprouting percentage showed that the relative sprouting percentage was significantly correlated with functional markers myb10DDFR-BVp1B3MFT-3A and MFT-A2, but not with PM19-A1MKK3-A and QSD1. These results indicated that molecular markers myb10DDFR-BVp1B3MFT-3A and MFT-A2 could be used for detection of pre-harvest sprouting resistance and marker-assisted breeding. Based on the phenotype and genotype results, nine white wheat germplasm resources with pre-harvest sprouting resistance were selected, including Yunong 914, Yunong 946, Fengdecunmai 30, Fangmai 5, Xumai 029, Lianmai 1901, Baofeng 1903, Zhengmai 829 and 13wang27-8, which could be used for genetic breeding and layout of wheat varieties with pre-harvest sprouting resistance.

Key words

wheat / germplasm resources / pre-harvest sprouting / molecular marker

Cite this article

Download Citations
GONG Chengru,YUAN Yuhao,LIU Zhen,ZHENG Jizhou,TIAN Zhicheng,LIU Shengli,SHEN Qinghua,HUANG Zhenpu,DONG Chunhao,GAO Yan,LI Qiaoyun,TANG Jianwei,JIAO Zhuqing,YIN Guihong. Preharvest Sprouting Resistance Test and Functional Markers Assisted Genotyping in Wheat Germplasm Resources. Journal of Plant Genetic Resources. 2024, 25(9): 1493-1503 https://doi.org/10.13430/j.cnki.jpgr.20231113002

References

[1]肖世和. 小麦穗发芽研究. 北京:中国农业科学技术出版社, 2004:2-41, 66-83Xiao S H. Studies on pre-harvest sprouting of wheat. Beijing: China Agricultural Science and Technology Press, 2004:2-41, 66-83
[2] Groos C, Gay G, Perretant M R, Gervais L, Bernard M, Dedryver F, Charmet D. Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white×red grain bread-wheat cross. Theoretical and Applied Genetics, 2002, 104(1):39-47
[3] Gubler F, Millar A A, Jacobsen J V. Dormancy release, ABA and pre-harvest sprouting. Current Opinion in Plant Biology, 2005, 8(2):183-187
[4]李玉营, 马东方, 王晓玲, 方正武. 小麦穗发芽鉴定方法的比较与分析. 广西植物, 2016, 36(3):261-266Li Y Y, Ma D F, Wang X L, Fang Z W. Comparison and analysis of wheat pre-harvest sprouting screening methods. Guihaia, 2016, 36(3):261-266
[5]王志龙, 于亚雄, 王志伟, 程加省, 乔祥梅, 杨金华. 小麦穗发芽抗性鉴定及机制分析. 西南农业学报, 2016, 29(11):2513-2519Wang Z L, Yu Y X, Wang Z W, Cheng J S, Qiao X M, Yang J H. Resistance and mechanism of pre-harvest sprouting in wheat. Southwest China Journal of Agricultural Science, 2016, 29(11):2513-2519
[6]苏在兴, 高闰飞, 易媛, 王波, 常勇, 黄忠勤, 周兴根. 小麦穗发芽抗性分析及相关分子标记检测. 麦类作物学报, 2019, 39(10):1173-1179Su Z X, Gao R F, Yi Y, Wang B, Chang Y, Huang Z Q, Zhou X G. Analys is of pre-harvest sprouting (PHS) resistance and detection on PHS associated molecular markers in wheat (Triticum aestivum L.). Journal of Triticeae Crops, 2019, 39(10):1173-1179
[7]刘光辉, 杨随庄, 黄可兵, 杨仕雷, 邢国风. 四川小麦穗发芽抗性鉴定及5个分子标记的有效性检验. 麦类作物学报, 2015, 35(2):167-174Liu G H, Yang S Z, Huang K B, Yang S L, Xing G F. Identification and validation of five molecular markers for PHS tolerence in sichua wheat varieties(lines). Journal of Triticeae Crops, 2015, 35(2):167-174
[8] Himi E, Maekawa M, Miura H, Noda K. Development of PCR markers for Tamyb10 related to R-1,red grain color gene in wheat. The Oretical and Applied Genetics, 2011, 122(8):1561-1576
[9] Himi E, Noda K. Isolation and location of three homoeologous dihydroflavonol-4-reductase(DFR) genes of wheat and their tissue-dependent expression. Journal of Experimental Botany, 2004, 55(396):365-375
[10] Bi H H, Sun Y W, Xiao Y G, Xia L Q. Characterization of DFR allelic variations and their associations with pre-harvest sprouting resistance in a set of red-grained Chinese wheat germplasm. Euphytica, 2014, 195(2):197-207
[11] Himi E, Noda K. Red grain colour gene (R) of wheat is a Myb-type transcription factor. Euphytica, 2005, 143(3):239-242
[12] Yang Y, Zhao X L, Xia L Q, Chen X M, Xia X C, Yu Z, He Z H, Roeder M. Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Theoretical and Applied Genetics, 2007, 115(7):971-980
[13] Jones S S, Warner R L, Kudrna D A, Spaeth S C. Dormancy in white-grain mutants of Chinese spring wheat(Triticum aestivum L.). Seed Science Research,2000,10(1):51-60
[14] Himi E, Mares D J, Yanagisawa A, Noda K. Effect of grain colour gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid(ABA)in wheat. Journal of Experimental Botany, 2002, 53(374):1569-1574
[15] Himi E, Nisar A. Colour genes(Rand Rc) for grain and coleoptile upregulate flavonoid biosynthesis genes in wheat. Genome, 2005, 48(4):747-754
[16] Nakamura S, Toyama T. Isolation of a VP1 homologue from wheat and analysis of its expression in embryos of dormant and non-dormant cultivars. Journal of Experimental Botany, 2001, 52(357): 875-876
[17] Mckibbin R S, Wilkinson M D, Bailey P C, Flintham J E, Andrew L M, Lazzeri P A, Gale M D, Lenton J R, Holdsworth M J. Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(15):10203-10208
[18] Xia L Q, Ganal M W, Shewry P R, He Z H, Yang Y, Roeder M S. Exploiting the diversity of Viviparous-1 gene associated with pre-harvest sprouting tolerance in European wheat varieties. Euphytica, 2008, 159:411-417
[19] Chang C, Feng J M, Si H Q, Yin B, Zhang H P, Ma C X. Validating a novel allele of viviparous-1(Vp-1Bf) associated with high seed dormancy of Chinese wheat landrace, Wanxianbaimaizi. Molecular Breeding, 2010, 25(3):517-525
[20] Chang C, Zhang H P, Feng J M, Yin B, Si H Q, Ma C X. Identifying alleles of Viviparous-1B associated with pre-harvest sprouting in micro-core collections of Chinese wheat germplasm. Molecular Breeding, 2010, 25(3):481-490
[21] Chang C, Zhang H P, Zhao Q X, Feng J M, Si H Q, Lu J, Ma C X. Rich allelic variations of Viviparous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat. Euphytica, 2011, 179(2):343-353
[22] Sun Y W, Jones H D, Yang Y, Dreisigacker S, Li S M, Chen X M, Shewry P R, Xia L Q. Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm. Euphytica, 2012, 186(1):25-43
[23] Yang Y, Zhang C L, Liu S X, Sun Y Q, Meng J Y, Xia L Q. Characterization of the rich haplotypes of Viviparous-1A in Chinese wheats and development of a novel sequence tagged site marker for pre-harvest sprouting resistance. Molecular Breeding, 2014, 33(1):75-88
[24] Sun Y W, Yang Y, Shewry P R, Jones H D, Xia L Q. Isolation and characterization of Viviparous-1 haplotypesin wheat related species. Euphytica, 2012, 188(1):71-84
[25] Barrero J M, Cavanagh C, Verbyla K L, Tibbit J F G, Verbyla A P, Huang B E, Rosewarne G M, Stephen S, Wang P H, Whan A, Rigault P, Hayden M J, Gubler F. Transcriptomic analysis of wheat near-isogenic lines identifies PM19-A1 and A2 as candidates for a major dormancy QTL. Genome Biology, 2015, 16(1):93-111
[26] Mori M, Uchino N, Chono M, Kato K, Miura H. Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theoretical and Applied Genetics, 2005, 110(7):1315-1323
[27]赵良侠. 小麦抗穗发芽基因TaMFT-3A新功能标记开发和TaPTF1表达特性及分布规律分析. 合肥:安徽农业大学,2017Zhao L X. Development of anew functional marker for TaMFT-3A as well as analysis of expression and distribution of TaPTF1 associated with pre-harvest sprouting resistance in commom wheat. Hefei: Anhui Agricultural University, 2017
[28] Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H, Mori M, Kawaura K, Ogihara Y, Miura H. A wheat homolog of MOTHER OF FT AND TFL1 acts in the regulation of germination. The Plant Cell, 2011, 23(9):3215-3229
[29] Liu S B, Sehgal S K, Li J R, Lin M, Trick H N, Yu J M, Gill B S, Bai G H. Cloning and characterization of a critical regulator for preharvest sprouting in wheat. Genetics, 2013, 195(1): 263-273
[30] Torada A, Koike M, Ogawa T, Takenouchi Y, Tadamura K, Wu J Z, Matsumoto T, Kawaura K, Ogihara Y. A causal gene for seed dormancy on wheat chromosome 4A encodes a MAP kinase kinase. CurrentBiology, 2016, 26(6):782
[31] Shorinola O, Balcrkov B, Hyles J, Tibbit J F G, Hayden M J, Holusova K, Valarik M, Distelfeld A, Torada A, Barrero J M, Uauy C. Haplotype analysis of the pre-harvest sprouting resistance locus Phs-A1 reveals a causal role of TaMKK3-A in global germplasm. Frontiers in Plant Science, 2017, 8:1555
[32]未文新. 小麦种子休眠位点鉴定及TaQsd1基因克隆和功能标记开发. 合肥:安徽农业大学, 2019Wei W X. Identification of wheat seed dormancy site, cloning of TaQsd1 gene and development of functional markers. Hefei: Anhui Agricultural University, 2019
[33]曹雪连, 张衡, 姜昊, 吴曾云, 曹佳佳, 朱玉磊, 王升星, 常成, 张海萍, 马传喜. 分子标记PM19-A1对1015份小麦抗穗发芽基因型的筛选及其有效性验证. 麦类作物学报, 2016, 36(10):1283-1290Cao X L, Zhang H, Jiang H, Wu Z Y, Cao J J, Zhu Y L, Wang S X, Chang C, Zhang H P, Ma C X. Screening and validation of 1015 wheat panicle sprouting resistance genotypes with molecular marker PM19-A1. Journal of Wheat Crops, 2016, 36(10):1283-1290
[34]王瑜, 王晓丽, 孙晓燕, 刘进英, 杨燕. 分子标记Tamyb10D和TaDFR-B的有效性验证及对小麦抗穗发芽基因型的筛选. 华北农学报, 2016, 31(1):63-70Wang Y, Wang X L, Sun X Y, Liu J Y, Yang Y. Validation of molecular markers Tamyb10D and TaDFR-B and screening of pre-harvest sprouting resistance genotypes in wheat. North China Journal of Agronomy, 2016, 31(1):63-70
[35]国家小麦良种重大科研联合攻关组、国家小麦产业技术体系. 小麦品种穗发芽抗性鉴定标准(T/GJXMLZ-CARS-2020). 北京:中国标准出版社,2021The National Major Scientific Research Joint Research Group for Improved Wheat Varieties and China Agriculture Research System. Identification standard for pre-harvest sprouting resistance of wheat(T/GJXMLZ-CARS-2020). Beijing: Standards Press of China, 2021
[36] Springer N M. Isolation of plant DNA for PCR and genotyping using organic extraction and CTAB. Cold Spring Harbor Protocols, 2010(11):10.1101
[37]王晓丽. Tamyb10基因在不同穗发芽抗性白粒小麦中等位变异的鉴定和分子标记研发. 呼和浩特:内蒙古农业大学, 2014Wang X L. Identification and development of a Tamyb10 STS marker for pre-harvest sprouting tolerance in Chinese wheat. Hohhot: Inner Mongolia Agricultural University, 2014
[38]王根平, 毕惠惠, 孙永伟, 王成社, 夏兰琴. 红粒小麦Tamyb10单倍型检测及其与穗发芽抗性的关系. 作物学报, 2014, 40(6):984-993Wang G P, Bi H H, Sun Y W, Wang C D, Xia L Q. Characterization of Tamyb10 haplotypes and their association with pre-harvest sprouting resistance in a set of Chinese red-grained wheats. Acta Agronomica Sinica, 2014, 40(6):984-993
[39] Wang Y, Wang X L, Meng J Y, Zhang Y J, He Z H, Yang Y. Characterization of Tamyb10 allelic variants and development of STS marker for pre-harvest sprouting resistance in Chinese bread wheat. Molecular Breeding, 2016, 36(11):148
[40]李亚青, 张楠, 彭义峰, 张士昌, 李孟军. 穗发芽抗性相关分子标记在优质小麦中的有效性验证. 河南农业科学, 2021, 50(10):18-26Li Y Q, Zhang N, Peng Y F, Zhang S C, Li M J. Validation of molecular markers related to pre-harvest sprouting resistance in high-quality wheat varieties. Journal of Henan Agricultural Sciences, 2021, 50(10):18-26

Funding

Foundation projects: Major Science and Technology Project of Henan Province in 2022(221100110700); Henan Province Agricultural Improved Breeds Joint Research Project (2022010102); Modern Agricultural Industry Technology System Construction Project of Henan Province (HARS-22-01-G2); Henan Provincial Science and Technology R&D Plan Joint Fund (222103810002); Major Project of Henan Province Industrial Research and Development Joint Fund in 2022 (225101610048); Top Talent Project of Henan Agricultural University (30500678)
Share on Mendeley
PDF(973 KB)

72

Accesses

0

Citation

Detail

Sections
Recommended

/