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Scientia Agricultura Sinica
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中文
Figure/Table detail
Function of Copper-Resistant Gene
copA
of
Ralstonia solanacearum
WANG XiaoNing,LIANG Huan,WANG Shuai,FANG WenSheng,XU JingSheng,FENG Jie,XU Jin,CAO AoCheng
Scientia Agricultura Sinica
, 2019, 52(
5
): 837-848. DOI:
10.3864/j.issn.0578-1752.2019.05.006
Fig. 1
Constituent components of GEN Ⅲ MicroPlant
Other figure/table from this article
Fig. 3
PCR detection of
copA
gene deletion and complementary strains
Fig. 8
Biolog metabolic microarray detection
Purple pore is defined as positive and pores shallower than purple are defined as boundary values. The picture indicates the metabolic results of Po82, ΔPo82copA and pBBR-ΔPo82copA strains in 96 h, respectively. The OD
590
of
α
-D-glucose (micropore plate C1) in the three strains is 0.632, 0.521 and 0.372, respectively. The OD
590
of D-trehalose (micropore plate A4) in the three strains is 0.620, 0.480 and 0.383, respectively. The OD
590
of L-alanine (micropore plate E3) in the three strains is 0.786, 0.370 and 0.436, respectively. The OD
590
of glucuronamide (micropore plate F6) in the three strains is 0.566, 0.388 and 0.380, respectively
Table 1
Strains and plasmids used in this study
Fig. 2
Phylogenetic tree based on
copA
sequence
Fig. 4
Induced expression of
copA
The asterisk on the column indicates significant difference in gene expression under different inductive concentrations (
P
<0.05)
Fig. 5
Growth curve test of the wild-type Po82, ΔPo82copA and pBBR-ΔPo82copA strains
* indicates significant difference between the mutant strain and wild-type strain under the same treatment (0.01<
P
≤0.05); ** indicates extremely significant difference between the mutant strain and wild-type strain under the same treatment (
P
<0.01). The same as below
Table 2
Primer sequences used in this study
Fig. 6
Pathogenicity test of the wild-type Po82, ΔPo82copA and pBBR-ΔPo82copA strains on tomato
Fig. 7
Expression analysis of pathogenic genes in the wild-type Po82 and ΔPo82copA strains
Fig. 9
The mobility of the wild-type Po82, ΔPo82copA and pBBR-ΔPo82copA strains
