Scientia Agricultura Sinica

Figure/Table detail

Function of Copper-Resistant Gene copA of Ralstonia solanacearum

WANG XiaoNing,LIANG Huan,WANG Shuai,FANG WenSheng,XU JingSheng,FENG Jie,XU Jin,CAO AoCheng

Scientia Agricultura Sinica, 2019, 52(5): 837-848. DOI: 10.3864/j.issn.0578-1752.2019.05.006

菌株和质粒 Strains and plasmids		特征 Characteristics	来源 Source
菌株 Strain	R. solanacearum Po82	Wild-type potato strain; Phylotype Ⅱ, sequevar. 4 (race 3 biovar. 2)	本实验室The laboratory
	E. coli DH5α	mcrA φ80 lacZΔM15, recA1, endA1	本研究This study
	ΔPo82copA	copA deletion strain, Gm^r	本研究This study
	PBBR-ΔPo82copA	copA complentmentary strain, Gm^r, Amp^r	本研究This study
质粒 Plasmid	pKMS1-gm (+U)	Cloning vector, Gm^r, Kan^r	本实验室The laboratory
	pBBR1MCS-4	Amp^r, lacZ alpha	本实验室The laboratory
	pKMS1-copA-gm	Gm^r, for copA gene deletion	本研究This study
	pBBR-copA	Amp^r, for copA gene complementation	本研究This study

Table 1 Strains and plasmids used in this study

Other figure/table from this article

Fig. 3 PCR detection of copA gene deletion and complementary strains
Fig. 8 Biolog metabolic microarray detection
Purple pore is defined as positive and pores shallower than purple are defined as boundary values. The picture indicates the metabolic results of Po82, ΔPo82copA and pBBR-ΔPo82copA strains in 96 h, respectively. The OD₅₉₀of α -D-glucose (micropore plate C1) in the three strains is 0.632, 0.521 and 0.372, respectively. The OD₅₉₀of D-trehalose (micropore plate A4) in the three strains is 0.620, 0.480 and 0.383, respectively. The OD₅₉₀of L-alanine (micropore plate E3) in the three strains is 0.786, 0.370 and 0.436, respectively. The OD₅₉₀of glucuronamide (micropore plate F6) in the three strains is 0.566, 0.388 and 0.380, respectively
Fig. 1 Constituent components of GEN Ⅲ MicroPlant
Fig. 2 Phylogenetic tree based on copA sequence
Fig. 4 Induced expression of copA
The asterisk on the column indicates significant difference in gene expression under different inductive concentrations (P <0.05)
Fig. 5 Growth curve test of the wild-type Po82, ΔPo82copA and pBBR-ΔPo82copA strains
* indicates significant difference between the mutant strain and wild-type strain under the same treatment (0.01<P ≤0.05); ** indicates extremely significant difference between the mutant strain and wild-type strain under the same treatment (P <0.01). The same as below
Table 2 Primer sequences used in this study
Fig. 7 Expression analysis of pathogenic genes in the wild-type Po82 and ΔPo82copA strains
Fig. 9 The mobility of the wild-type Po82, ΔPo82copA and pBBR-ΔPo82copA strains
Fig. 6 Pathogenicity test of the wild-type Po82, ΔPo82copA and pBBR-ΔPo82copA strains on tomato

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