本研究以冰草属（Agropyron Gaertn.）中的一个优质种间杂种——“蒙农杂种”冰草（A. cristatum хA.desertorum cv.‘Hycrest-Mengnong’ ）为材料，在以幼穗为外植体建立的冰草组织培养再生体系基础上，以调控脯氨酸生物合成最后一步的关键酶的突变体基因p5CS为目标基因，bar基因为筛选标记基因，采用共转化法利用基因枪轰击冰草幼穗诱导的愈伤组织获得转基因植株,为冰草的遗传改良提供新种质。PCR和Southern检测表明外源基因p5CS已整合到冰草植物基因组DNA中；RT-PCR检测表明目的基因已在冰草转基因植株的转录水平表达；p5CS基因的遗传转化率为0.11%。

The transgenic wheatgrasses were investigated in the case of a hybrid wheatgrass (A. cristatum х A. desertorum cv. ‘Hycrest-Mengnong’). Based on the established regeneration system, the p5CS gene, which regulates the last step of proline synthesis in plant, was transformed into wheatgrass with phosphinothricin acetyltransferase (bar) conferring herbicide resistance as selecting gene. The transformation was conducted through microprojectile bombardment of callus derived from immature inflorescence by co-transformation. The obtained transgenic plants can provide the new germplasm resource for wheatgrass genetically improvement.The results of PCR and Southen analysis displayed that the exogenous p5CS gene had integrated into the genome of transgenic wheatgrass.The assay of RT-PCR showed the transgenic p5CS had expressed at transcript level.The transgenic frequencies of p5CS gene were 0.11%.