小麦抗叶锈病基因Lr45的AFLP分子标记

张娜,刘大群,杨文香,闫红飞,褚 栋,孟庆芳,张 汀

中国农业科学. 2005, 38(07): 1364-1368

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PDF(296 KB)
中国农业科学 ›› 2005, Vol. 38 ›› Issue (07) : 1364-1368. DOI: 10.3864/j.issn.0578-1752.at-2004-2996
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小麦抗叶锈病基因Lr45的AFLP分子标记

  • 张娜,刘大群,杨文香,闫红飞,褚 栋,孟庆芳,张 汀
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Molecular Markers of Wheat Leaf Rust Resistance Gene Lr45 Based on AFLP

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摘要

利用AFLP技术对小麦抗叶锈基因Lr45进行了标记,从60对AFLP引物中筛选出2对在亲本及TcLr45×Thatcher F2抗感群体间揭示多态性的引物P-AGG/M-GAG和P-ACA/M-GGT。其扩增片段为261 bp和105 bp,2个标记与Lr45的遗传距离分别为0.6 cM和1.3 cM。测序比较261 bp片段与大麦属Vulgare HotrI基因部分序列同源性达86%,105 bp片段与一粒小麦磷脂酰丝氨酸脱羧酶基因部分序列同源性高达96%。2个测序片段均包含开放阅读框(ORF)序列。

Abstract

AFLP technology was employed to isolate wheat leaf rust resistance gene Lr45. Sixty primer pairs were screened, of which two primer pairs, P-AGG/M-GAG and P-ACA/M-GGT, displayed polymorphsim in the population of TcLr45, Thatcher, and their F2 generation. Two markers were found tightly linked to Lr45. One links to the Lr45 locus with a distance of 0.6 cM, and the other with a distance of 1.3 cM. The fragement of 261 bp was 86 percent the same as a part of the original sequence of Vulgare HortI gene, and the 105 bp was 96 percent compared with a part of the triticum monococcum phosphatidylserine decarboxylase gene. Both of them included an open reading frame (ORF).

关键词

小麦叶锈病 / AFLP / Lr45 / 基因定位

Key words

Wheat leaf rust / AFLP / Lr45 / Gene localization

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张娜,刘大群,杨文香,闫红飞,褚 栋,孟庆芳,张 汀. 小麦抗叶锈病基因Lr45的AFLP分子标记. 中国农业科学. 2005, 38(07): 1364-1368 https://doi.org/10.3864/j.issn.0578-1752.at-2004-2996
,,,,,,. Molecular Markers of Wheat Leaf Rust Resistance Gene Lr45 Based on AFLP. Scientia Agricultura Sinica. 2005, 38(07): 1364-1368 https://doi.org/10.3864/j.issn.0578-1752.at-2004-2996
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