AFLP technology was employed to isolate wheat leaf rust resistance gene Lr45. Sixty primer pairs were screened, of which two primer pairs, P-AGG/M-GAG and P-ACA/M-GGT, displayed polymorphsim in the population of TcLr45, Thatcher, and their F2 generation. Two markers were found tightly linked to Lr45. One links to the Lr45 locus with a distance of 0.6 cM, and the other with a distance of 1.3 cM. The fragement of 261 bp was 86 percent the same as a part of the original sequence of Vulgare HortI gene, and the 105 bp was 96 percent compared with a part of the triticum monococcum phosphatidylserine decarboxylase gene. Both of them included an open reading frame (ORF).
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Molecular Markers of Wheat Leaf Rust Resistance Gene Lr45 Based on AFLP. Scientia Agricultura Sinica. 2005, 38(07): 1364-1368 https://doi.org/10.3864/j.issn.0578-1752.at-2004-2996