将孔雀草品种T. patula'Little Hero Golden'的叶片、子叶和下胚轴接种于4种含有不同浓度IAA、NAA和BA的MS培养基上，比较不同外植体的再生植株的能力。结果表明，叶片再生植株能力较子叶和下胚轴强。随后，进一步将其叶片接种于9种添加不同浓度NAA和BA的MS培养基上，分化结果显示，在MS+NAA 1.5 mg·L-1+BA 1.0 mg·L-1的培养基上植株再生率最高，达75%。5份材料的叶片分别接种在该培养基上，再生率均达70%以上，其中T. patula21614的再生率达85%。所有再生植株均能成苗并表现出正常的形态特征。从而建立了适合于５种基因型孔雀草的离体高效再生体系。

In order to test the regeneration ability of different explants, the leaves, cotyledons and hypocotyls ofT. patula'Little Hero Golden' were cultured on 4 kinds of media, MS + NAA 0.5 mg·L-1+ BA 1.0 mg·L-1, MS + NAA 1.0mg·L-1 + BA 2.0 mg·L-1, MS + NAA 1.5 mg·L-1 + BA 2.0 mg·L-1 and MS + IAA 3.0 mg·L-1 + BA 2.5 mg·L-1. The results showed that the regeneration ability of leaves was higher than that of cotyledons and hypocotyls. The leaves were then cultured on 9 kinds of MS media with different combinations of NAA and BA, and the highest regeneration frequency was obtained on MS medium supplemented with 1.5 mg·L-1NAA and 1.0 mg·L-1 BA, and the regeneration rate was up to 75%. The medium was tested for other 4 genotypes, and all the lines were successfully regenerated with frequencies from 70% to 85%. All the regenerated plantlets developed into complete plants of normal morphological characteristics.