BcSDR1 is involved in regulation of glucose transport and cAMP and MAPK signaling pathways in Botrytis cinerea

SI He-long, ZHANG Kang, LI Bai, YUAN Xue-mei, ZANG Jin-ping, CAO Hong-zhe, XING Ji-hong, DONG Jin-gao

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (9) : 2628-2640. DOI: 10.1016/j.jia.2022.07.027
Plant Protection

BcSDR1 is involved in regulation of glucose transport and cAMP and MAPK signaling pathways in Botrytis cinerea

  • SI He-long2*, ZHANG Kang1, 2*, LI Bai1, 2*, YUAN Xue-mei2, ZANG Jin-ping2, CAO Hong-zhe2, XING Ji-hong1, 2, DONG Jin-gao1, 2

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Abstract

Botrytis cinerea is a typical necrotrophic pathogenic fungus that causes severe diseases in a wide range of plant species, leading to significant economic losses.  Our previous study showed that BcSDR1 positively regulates growth, development, and pathogenicity of Bcinerea.  However, the regulation mechanism of BcSDR1 and the relationship between BcSDR1 and cAMP and MAPK signaling pathways are not well understood.  In this study, transcriptome data showed that BcSDR1 is involved in glucose transmembrane transport, signal transduction, secondary metabolism, and other biological processes.  BcSDR1 mutant (BCt41) showed remarkably weak sensitivity to cAMP and MAPK signaling pathways specific inhibitors, SQ22536 and U0126, and significantly decreased cAMP content.  The key genes of cAMP and MAPK signaling pathways, BcGB1, BcBTP1, BcBOS1, BcRAS1, and BcBMP3 were significantly upregulated, whereas BcPLC1, BcBCG1, BcCDC4, BcSAK1, BcATF1, and BcBAP1 were significantly downregulated (P<0.05).   BcSDR1 was obviously upregulated in BcBCG2, BcBCG3, BcPKA1, and BcPKAR RNA interference (RNAi)  mutants, but significantly downregulated in BcPKA2, BcBMP1, and BcBMP3 RNAi mutants.  Thus, BcBCG2, BcBCG3, BcPKA1, and BcPKAR negatively regulate BcSDR1 expression, whereas BcPKA2, BcBMP1, and BcBMP3 positively regulate BcSDR1 expression.

Key words

Botrytis cinerea / BcSDR1 / glucose transmembrane transport / cAMP signaling pathway / MAPK signaling pathway

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SI He-long, ZHANG Kang, LI Bai, YUAN Xue-mei, ZANG Jin-ping, CAO Hong-zhe, XING Ji-hong, DONG Jin-gao. BcSDR1 is involved in regulation of glucose transport and cAMP and MAPK signaling pathways in Botrytis cinerea. Journal of Integrative Agriculture. 2022, 21(9): 2628-2640 https://doi.org/10.1016/j.jia.2022.07.027

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Funding

This study was supported by the National Natural Science Foundation of China (31972217 and 32072369), the Central Government Guides Local Science and Technology Development Projects, China (206Z6501G and 216Z6502G), and the Research Project of Basic Scientific Research Business Fees in Provincial Universities of Hebei Province, China (KY2021043 and KY2021044).

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