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Diversity of Bacterial Community Structure of Toona Sinensis Rhizosphere Soil in Taihe Area
ZHAOMingxia, CHENQian, LIUXiaoli, WEIBing, ZHOUZuoqin, SANGYangli, ZHANGQianqian, QUChangqing
PDF(2157 KB)
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Diversity of Bacterial Community Structure of Toona Sinensis Rhizosphere Soil in Taihe Area
To investigate the soil bacterial community structure of the Toona sinensis cultivation area in Taihe, soil samples from the rhizosphere of Toona sinensis from Taihe were collected. The 16S rDNA V3-V4 region of soil bacteria was sequenced using high-throughput sequencing technology on the Illumina HiSeq platform, and bioinformatics analysis was performed based on the data. The results showed that a total of 1628 operational taxonomic units (OTUs) were obtained, belonging to 26 phyla, 63 classes, 149 orders, 261 families, 512 genera and 574 species. At the phylum level, the dominant bacteria in the rhizosphere soil of Toona sinensis were Proteobacteria (24.26%), Actinobacteria (22.97%) and Firmicutes (21.50%). COG and KEGG annotation analysis of microbiota gene function showed that rhizosphere soil colonies of Toona sinensis could synthesize natural products, such as terpenoids, type II polyketones, aminoglycosides, β-lactams, steroids, non-ribosomal peptides, etc. These results indicated that the bacteria in the rhizosphere soil of Toona sinensis in Taihe not only had rich diversity but also could produce structure-rich and active natural products. The bacteria in the rhizosphere soil of Toona sinensis had great potential for discovering new active natural compounds. The results of this study are of great significance for the utilization and development of microbial resources in the rhizosphere soil of Toona sinensis.
Toona sinensis cultivated in Taihe / Illumina HiSeq / rhizosphere soil / bacterial diversity / natural products {{custom_keyword}} /
表1 各分类水平的微生物类群数统计 |
| 样品 | 门 | 纲 | 目 | 科 | 属 | 种 |
|---|---|---|---|---|---|---|
| S1 | 24 | 60 | 146 | 252 | 494 | 551 |
| S2 | 24 | 60 | 143 | 247 | 488 | 548 |
| S3 | 25 | 60 | 145 | 256 | 498 | 556 |
| S4 | 24 | 59 | 143 | 246 | 480 | 537 |
| S5 | 26 | 61 | 137 | 226 | 432 | 479 |
| S6 | 25 | 61 | 133 | 228 | 438 | 484 |
| S7 | 25 | 61 | 136 | 230 | 428 | 474 |
| 总计 | 26 | 63 | 149 | 261 | 512 | 574 |
表2 样本细菌Aplha多样性统计 |
| 样品 | 分类操作单元(OUT) | Ace丰富度(ACE) | Chao丰富度(Chao1) | 香农指数(Shannon) | 覆盖率/% |
|---|---|---|---|---|---|
| S1 | 1540 | 1577.76 | 1587.36 | 8.66 | 99.76 |
| S2 | 1460 | 1528.89 | 1548.00 | 8.52 | 99.66 |
| S3 | 1535 | 1582.30 | 1603.98 | 8.52 | 99.74 |
| S4 | 1448 | 1510.45 | 1537.06 | 8.44 | 99.65 |
| S5 | 1297 | 1372.77 | 1384.29 | 4.57 | 99.73 |
| S6 | 1311 | 1336.28 | 1353.66 | 8.62 | 99.87 |
| S7 | 1292 | 1333.20 | 1360.62 | 8.65 | 99.82 |
| 平均 | 1412 | 1463.09 | 1482.14 | 8.00 | 99.75 |
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PDF(2157 KB)
表1 各分类水平的微生物类群数统计
图1 样本细菌的Shannon-稀释曲线表2 样本细菌Aplha多样性统计图2 门和纲水平上细菌类群的组成及相对丰度图3 属水平上细菌类群的组成及相对丰度图4 属水平各物种网络图图5 细菌的COG功能分类/
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