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Identification and Evaluation of Field Resistance to Rice Blast in Japonica Rice Cultivars
CHENJianchen, DONGLiying, LIUPei, TANGCuifeng, SONGJie, XIANGJinqian, LIUShufang, YULei, YANGQinzhong
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Identification and Evaluation of Field Resistance to Rice Blast in Japonica Rice Cultivars
In order to compare the differences of resistance to rice blast disease in Yunnan among japonica rice cultivars collected from 17 provinces, and to screen favorable resistant resources of rice, we employed the method of natural induction in field blast nursery to assess the resistance of 1050 japonica rice cultivars against leaf and panicle blast. The results indicated that among the 1050 japonica rice cultivars, there was lighter incidence of leaf blast and more severe incidence of panicle blast. Regarding leaf blast resistance, the cultivars exhibiting high resistance, resistance, moderate resistance, moderate susceptibility, susceptibility, and high susceptibility accounted for 28.48%, 10.95%, 9.14%, 19.24%, 20.00%, and 12.19%, respectively. As for panicle blast resistance, the cultivars exhibiting high resistance, resistance, moderate resistance, moderate susceptibility, susceptibility, and high susceptibility accounted for 3.71%, 0.38%, 2.57%, 19.62%, 22.57%, and 51.14%, respectively. Further analysis of the leaf and panicle blast results revealed that 69 japonica rice cultivars had resistance to both leaf and panicle blast. These 69 cultivars were collected from five provinces of Jilin, Heilongjiang, Liaoning, Yunnan, and Jiangsu. These results suggested that these cultivars with good resistance against both leaf and panicle blast in blast nursery could be served as important resistance resources for blast disease-resistant rice breeding, and discovery of new genes conferring resistance to rice blast.
japonica rice cultivars / rice blast / resistance / evaluation / leaf blast / panicle blast / breeding resources {{custom_keyword}} /
| [1] |
杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展[J]. 中国农业科学, 2009, 42(5):1601-1615.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [2] |
马会珍. 不同生态区优质食味粳稻品质特征的比较研究[D]. 扬州: 扬州大学, 2021.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [3] |
高清, 张亚玲, 周弋力, 等. 黑龙江省粳稻品种稻瘟病主效抗性基因鉴定与抗性评价[J]. 作物杂志, 2021(4):59-66.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [4] |
王东元. 粳稻遗传多样性分析及抗稻瘟病分子辅助育种[D]. 天津: 天津农学院, 2021.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [5] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [6] |
聂燕芳, 冷梅钦, 李冠军, 等. 稻瘟菌二肽基肽酶V基因DppV的功能分析[J]. 植物保护学报, 2021, 48(4):712-722.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [7] |
王如意, 刘杰, 冯琴, 等. 稻瘟病抗性分子机制及抗病育种策略研究进展[J]. 植物保护, 2020, 49(5):32-42.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [8] |
姜少芸, 郑露, 朱昳, 等. 湖北省主栽水稻品种对稻瘟病的田间抗性鉴定[J]. 南方农业学报, 2016, 47(8):1303-1312.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [9] |
邹秋霞. 稻瘟病生防菌的分离鉴定与防控效果评价[D]. 长沙: 湖南农业大学, 2018.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [10] |
朱小源, 杨健源, 刘景梅, 等. 广东水稻品种抗稻瘟病性分析与利用策略[J]. 广东农业科学, 2006(5):34-37.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [11] |
颜群, 韦鸿若, 罗志勇, 等. 广西岑溪田间自然诱发稻瘟病圃的建立及水稻品系的抗瘟性评价[J]. 中国农学通报, 2011, 27(27):255-259.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [12] |
董丽英, 李国生, 赵秀兰, 等. 云南粳稻品种(系)对稻瘟病的抗性鉴定与评价[J]. 西南农业学报, 2018, 31(12):2458-2465.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [13] |
李旭升, 向小娇, 申聪聪, 等. 水稻重测序核心种质资源的稻瘟病抗性鉴定与评价[J]. 作物学报, 2017, 43(6):795-810.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [14] |
刘树芳, 张先闻, 董丽英, 等. 云南不同稻区稻瘟病菌群体遗传结构的SSR分析[J]. 中国农学通报, 2023, 39(12):123-130.
为了解稻瘟病菌的群体遗传结构，选用13对SSR标记对2007—2013年分离自云南省陆稻区、籼稻区和粳稻区（共计13个州）的125个稻瘟病菌单孢菌株的全基因组DNA进行PCR扩增及分析，结果13对引物共检测出128个等位基因；聚类分析表明，在相似系数为0.57时，125个供试菌株可划分为29个遗传宗谱，优势宗谱YN02包含了39个菌株，占总菌株数的31.20%，次优势宗谱YN08包含了15个菌株，占总菌株数的12.00%，其余71个菌株分属27个宗谱；表明云南省既有优势宗谱，又有较多遗传多样性的小宗谱，菌株群体遗传多样性丰富；比较不同稻区菌株群体的宗谱频率发现，陆稻区菌株的宗谱频率为65.52%，粳稻区和籼稻区菌株的宗谱频率分别为27.59%和13.79%，表明陆稻区菌株群体的遗传结构比粳稻区、籼稻区菌株复杂。因此，针对菌株群体宗谱较少，且优势宗谱突出的地方，应加强品种多样化种植，有利于稻瘟病菌群体的稳定化选择，降低病害流行风险。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [15] |
董丽英, 刘树芳, 田维逵, 等. 云南省稻瘟病菌群体的致病性分析及交配型分布[J]. 植物保护学报, 2023, 50(2):316-324.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [16] |
田大刚, 苏军, 陈建民, 等. 1092份水稻材料稻瘟病抗性鉴定及抗性标记分析[J]. 分子植物育种, 2012, 10(2):214-221.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [17] |
徐志健, 农保选, 张宗琼, 等. 广西水稻地方品种核心种质抗稻瘟病鉴定及评价[J]. 南方农业学报, 2020, 51(5):1039-1046.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [18] |
周海平, 张帆, 陈凯, 等. 水稻种质资源稻瘟病抗性全基因组关联分析[J]. 作物学报, 2020, 49(5):1170-1183.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [19] |
杨秀荣, 闫双勇, 李月娇, 等. 黄淮稻区水稻品种稻瘟病抗性表现及Pib和Pi5抗性基因分析[J/OL]. 分子植物育种, http://kns.cnki.net/kcms/detail/46.1068.S.20220317.1509.014.html, 2024-05-11.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [20] |
刘树芳, 董丽英, 李迅东, 等. 持有Pi9基因的水稻单基因系IRBL9-W对稻瘟病菌苗期和成株期抗性差异[J]. 中国水稻科学, 2021, 35(3):303-310.
【目的】Pi9是一个广谱稻瘟病抗性基因,田间病圃监测发现持有Pi9的水稻单基因系IRBL9-W在苗期高抗稻瘟病,但却感穗瘟。探明水稻单基因系IRBL9-W在苗期抗病而孕穗末期感染穗颈瘟的原因,为Pi9基因在水稻抗病育种中的有效利用提供参考。【方法】利用从IRBL9-W穗颈瘟病斑上分离的8个单孢菌株以及实验室保存的单孢菌株Y363,在温室分别对单基因系IRBL9-W苗期和孕穗末期进行接种鉴定;并利用病原菌AvrPi9基因的特异引物对9个单孢菌株进行PCR扩增及产物测序;提取水稻单基因系IRBL9-W苗期叶片和抽穗期穗部的总RNA,通过半定量RT-PCR以及实时qRT-PCR分析Pi9基因在苗期和穗期的表达。【结果】在温室人工喷雾接种条件下,IRBL9-W在苗期对从穗颈瘟上分离的8个单孢及对照菌株Y363均表现为抗病;随机选取的2个从IRBL9-W穗颈瘟病样分离的单孢菌株(YX2-7-1和YX2-15-1)及对照菌株Y363对孕穗末期IRBL9-W注射接种,接种的植株表现出典型的穗颈瘟症状;AvrPi9的等位基因分析结果表明,与AvrPi9相比,Y363中的等位基因与AvrPi9完全相同,而从IRBL9-W穗瘟分离的8个单孢菌株中编码区与AvrPi9基因完全相同,但在编码起始位置上游-264 bp处缺失16 bp的一段序列。由于IRBL9-W苗期对这些菌株均表现抗病,推测这段序列的缺失并不影响AvrPi9基因的功能;实时qRT-PCR分析结果表明,Pi9基因在穗部的表达量为苗期叶片表达量的47.3%。【结论】在水稻单基因系IRBL9-W中,与苗期叶片中Pi9基因的表达量相比,Pi9基因在穗部表达量的明显降低可能是造成IRBL9-W穗期感稻瘟病的原因。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [21] |
高清, 张亚玲, 葛欣, 等. 水稻抗稻瘟病基因研究进展[J]. 分子植物育种, 2022, 20(11):3634-3643.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [22] |
相亚超, 王丽丽, 徐凡, 等. 抗稻瘟病基因在黑龙江水稻资源中的分布[J]. 分子植物育种, 2018, 16(23):7705-7717.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [23] |
何烈干, 马辉刚, 肖叶青, 等. 江西省水稻品种对稻瘟病的抗性鉴定与评价[J]. 江西农业大学学报, 2015, 37(2):278-283.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [24] |
周伍民, 董丽英, 刘树芳, 等. 抗稻瘟病基因特异分子标记开发及在云南粳稻中的应用[J/OL]. 分子植物育种, http://kns.cnki.net/kcms/detail/46.1068.s.20210928.0151.008.html,2024-05-11.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [25] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| {{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
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