Detection of Disease Resistance Genes in 233 Medium Fruit Tomato Isolates

WANGShanshan, WANGChenyu, YANGChaosha, CAIXiaoyi, YINQingzhen

PDF(3708 KB)
PDF(3708 KB)
Chinese Agricultural Science Bulletin ›› 2024, Vol. 40 ›› Issue (34) : 24-32. DOI: 10.11924/j.issn.1000-6850.casb2024-0012

Detection of Disease Resistance Genes in 233 Medium Fruit Tomato Isolates

Author information +
History +

Abstract

In order to screen the resources of medium fruit tomato with multiple resistances and provide material basis for tomato disease resistance breeding, using 28 medium fruit tomato varieties introduced by our research group as materials, 17 resistance genes of 12 diseases were detected by five-primer amplification inhibited mutation system (PARMS) method, including yellow leaf curl virus resistance genes Ty-1 and Ty-5, tobacco mosaic virus resistance gene Tm-2a, root nematode resistance genes Mi1.2 and Mi2.3, Fusarium crown and root rot resistance gene Frl, leaf mold resistance genes Cf-5 and Cf-9, grey leaf spot resistance genes Sm, wilt resistance gene I-2, late blight resistance gene Ph-3, bacterial wilt resistance genes Bwr12 and Bwr6, bacterial speck resistance gene Pto, bacterial spot resistance gene Rx4, Verticillium wilt resistance gene Ve1 and Ve2. Among the 233 isolates, there were 222, 215, 198 and 194 isolates containing the resistance genes Ve1, Sm, Ve2 and Mi1.2, respectively. The isolates with 7 and 8 resistance genes were 55 and 50, respectively. 140 isolates were with 8 or more resistance genes, accounting for 60%. 3 isolated strains containing 12 resistance genes were screened, which were 2-2, 5-4 and 21-3. And there were 21 isolated strains containing 11 resistance genes, which were 1-1, 1-3, 2-3, 2-4, 2-7, 2-8, 4-2, 4-4, 4-5, 5-7, 5-8, 8-1, 8-7, 8-8, 12-1, 12-2, 12-5, 18-7, 18-8, 20-8, 26-2. Polyresistant tomato resources were abundant. In this study, a number of medium fruit tomato resources with multiple antibodies were obtained, and the next step can be purification of the isolated single strain, providing a material basis for screening medium fruit tomato with multiple antibodies.

Key words

medium-fruit tomato / isolates / PARMS / resistance detection

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
WANG Shanshan , WANG Chenyu , YANG Chaosha , CAI Xiaoyi , YIN Qingzhen. Detection of Disease Resistance Genes in 233 Medium Fruit Tomato Isolates. Chinese Agricultural Science Bulletin. 2024, 40(34): 24-32 https://doi.org/10.11924/j.issn.1000-6850.casb2024-0012

References

Publishing order | Descend order by publishing year | Descend order by cited within
[1]
许向阳, 赵爽, 何梦曦, 等. 128份番茄种质资源主要农艺性状鉴定及抗病性评价[J]. 东北农业大学学报, 2022, 53(5):32-41.
本文引用 [1]
[2]
田永强, 高丽红. 设施番茄高品质栽培理论与技术[J]. 中国蔬菜, 2021(2):30-40.
本文引用 [1]
[3]
李天来. 我国设施蔬菜科技与产业发展现状及趋势[J]. 中国农村科技, 2016(5):75-77.
本文引用 [1]
[4]
杨超沙, 王国华, 吴志明, 等. 番茄黄化曲叶病毒及根结线虫抗性基因的检测[J]. 河北农业大学学报, 2018, 41(2):73-76.
本文引用 [2] 摘要
利用分子标记技术对203个番茄自交系分别进行了黄化曲叶病毒抗性基因TY-1，TY-2，TY-3以及根结线虫抗性基因的Mi-1的筛选分子鉴定结果表明，所检测材料中，含TY-1纯合材料有22个，杂合材料29个;含TY-2杂合材料1个;含TY-3个纯合材料有12个，杂合材料26个;含的Mi-1共纯合材料有16个，杂合材料9个。其中，同时含有3个抗性基因的共11个，同时含有2个抗性基因的有24个。利用分子检测，有助于快速有效地培育抗病，综合园艺性状良好的番茄品种，从而满足市场的需求
[5]
ZAMIR D, EKSTEIN-MICHELSON I, ZAKAY Y, et al. Mapping and introgression of a tomato yellow leaf curl virus tolerance gene, TY-1[J]. Theoretical and applied genetics, 1994, 88(2):141-146.
本文引用 [1]
[6]
YAMAGUCHI H, OHNISHI J, SAITO A, et al. An NB-LRR gene, TYNBS1, is responsible for resistance mediated by the Ty-2 Begomovirus resistance locus of tomato[J]. Theoretical and applied genetics, 2018, 131(6):1345-1362.
本文引用 [1]
[7]
JUNG J, KIM H J, LEE J M, et al. Gene-based molecular marker system for multiple disease resistances in tomato against tomato yellow leaf curl virus, late blight, and verticillium wilt[J]. Euphytica, 2015, 205(2):599-613.
本文引用 [1]
[8]
CARO M, VERLAAN M G, JULIÁN O, et al. Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm[J]. Molecular breeding, 2015, 35(6):132-145.
本文引用 [1]
[9]
EL MEHRACH K, GHARSALLAH CHOUCHANE S, MEJIA L, et al. PCR-based methods for tagging the mi1 locus for resistance to root-knot nematode in begomovirus-resistant tomato germplasm[J]. Acta horticulturae, 2005,695:263-270.
本文引用 [1]
[10]
SHI A, VIERLING R, GRAZZINI R, et al. Molecular markers for Tm-2 alleles of tomato mosaic virus resistance in tomato[J]. American journal of plant sciences, 2011, 2(2):180-189.
本文引用 [1]
[11]
张育垚, 李景富, 谢立波, 等. 番茄Cf-5基因的SNP分子标记开发[J]. 东北农业大学学报, 2012, 43(4):93-98.
本文引用 [1]
[12]
KIM B, HWANG I S, LEE H J, et al. Combination of newly developed SNP and InDel markers for genotyping the Cf-9 locus conferring disease resistance to leaf mold disease in the tomato[J]. Molecular breeding, 2017, 37(5):59-68.
本文引用 [1]
[13]
刘蕾, 王辉, 李文丽, 等. 与番茄颈腐根腐病抗病基因Frl连锁的标记及应用[J]. 江苏农业科学, 2018, 46(16):91-93.
本文引用 [1]
[14]
郝耀港, 宋建军, 李珂, 等. 与番茄灰叶斑病抗病基因Sm连锁的CAPS标记开发[J]. 中国瓜菜, 2023, 36(4):47-55.
本文引用 [1]
[15]
苏晓梅. 番茄抗灰叶斑病基因Sm的精细定位与克隆[D]. 北京: 中国农业大学,2018:1-77.
本文引用 [1]
[16]
EL MOHTAR C A, ATAMIAN H S, DAGHER R B, et al. Marker-assisted selection of tomato genotypes with the I-2 gene for resistance to Fusarium oxysporum f. sp. lycopersici Race 2[J]. Plant disease, 2007, 91(6):758-762.
本文引用 [1]
[17]
苏晓梅, 高建昌, 王孝宣, 等. 番茄抗病基因Tm-2、Pto、Sw-5和Ve1的SNP标记检测[J]. 园艺学报, 2014, 41(10):2012-2020.
本文引用 [1] 摘要
针对番茄4个抗病基因Tm-2、Pto、Sw-5和Ve1，根据其序列的碱基差异设计引物，经过引物特异性检测和PCR产物克隆测序比对之后，采用高分辨率熔解曲线（High resolution melting，HRM）技术进行多态性检测，共开发了5个SNP标记。经过验证，所开发的标记均能将抗病基因型不同的番茄材料分型，并且分型结果与已知材料的抗病性状完全一致。这些标记可以作为功能标记在番茄抗病育种中应用。
[18]
胡明瑜, 白文钦, 潘晓雪, 等. 番茄抗晚疫病基因Ph-3的分子标记开发及应用[J]. 植物病理学报2018, 48(4):560-566.
本文引用 [1]
[19]
KIM B, HWANG I S, LEE H J, et al. Identification of a molecular marker tightly linked to bacterial wilt resistance in tomato by genome-wide SNP analysis[J]. Theoretical and applied genetics, 2018, 131(5):1-14.
本文引用 [1]
[20]
ROSE L E; LANGLEY C H, BERNAL A J. et al. Natural variation in the Pto pathogen resistance gene within species of wild tomato (Lycopersicon). I. Functional analysis of Pto alleles[J]. Genetics, 2005, 171(1):345-357.
https://doi.org/10.1534/genetics.104.039339
https://www.ncbi.nlm.nih.gov/pubmed/15944360
本文引用 [1] 摘要
Disease resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) in the cultivated tomato, Lycopersicon esculentum, and the closely related L. pimpinellifolium is triggered by the physical interaction between plant disease resistance protein, Pto, and the pathogen avirulence protein, AvrPto. To investigate the extent to which variation in the Pto gene is responsible for naturally occurring variation in resistance to Pst, we determined the resistance phenotype of 51 accessions from seven species of Lycopersicon to isogenic strains of Pst differing in the presence of avrPto. One-third of the plants displayed resistance specifically when the pathogen expressed AvrPto, consistent with a gene-for-gene interaction. To test whether this resistance in these species was conferred specifically by the Pto gene, alleles of Pto were amplified and sequenced from 49 individuals and a subset (16) of these alleles was tested in planta using Agrobacterium-mediated transient assays. Eleven alleles conferred a hypersensitive resistance response (HR) in the presence of AvrPto, while 5 did not. Ten amino acid substitutions associated with the absence of AvrPto recognition and HR were identified, none of which had been identified in previous structure-function studies. Additionally, 3 alleles encoding putative pseudogenes of Pto were isolated from two species of Lycopersicon. Therefore, a large proportion, but not all, of the natural variation in the reaction to strains of Pst expressing AvrPto can be attributed to sequence variation in the Pto gene.
[21]
PEI C, WANG H, ZHANG J, et al. Fine mapping and analysis of a candidate gene in tomato accession PI128216 conferring hypersensitive resistance to bacterial spot race T3[J]. Theoretical & applied genetics, 2012, 124(3):533-542.
本文引用 [1]
[22]
ACCIARRI N, ROTINO G L, TAMIETTI G, et al. Molecular markers for Ve1 and Ve2 Verticillium resistance genes from Italian tomato germplasm[J]. Plant breed, 2007, 126(6):617-621.
本文引用 [1]
[23]
SHU Y, SAHAR D, XIAYI K, et al. An efficient procedure for genotyping single nucleotide polymorphisms[J]. Nucleic acids research, 2001, 29(17):88-96.
https://www.ncbi.nlm.nih.gov/pubmed/11522844
本文引用 [1] 摘要
Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.
[24]
ZHANG B, ZHAO N, LIU Y, et al. Novel molecular markers for high-throughput sex characterization of Cynoglossus semilaevis[J]. Aquaculture, 2019,513:734331.
本文引用 [1]
[25]
石雪燕, 李涛, 王虹云, 等. PARMS分子标记检测方法在番茄种质资源鉴定中的应用[J]. 农业与技术, 2023, 43(17):25-29.
本文引用 [1]
[26]
杨超沙, 王珊珊, 尹伟平, 等. 八十一份番茄种质资源的六种病害抗病基因检测[J]. 北方园艺, 2022(20):1-9.
本文引用 [2]
[27]
张文慧. 陕南山区54个番茄地方品种抗病基因鉴定[J]. 陕西农业科学, 2022, 68(3):43-45.
本文引用 [1]
[28]
范惠冬, 许世霖, 郑士金, 等. 105份番茄种质资源的抗性基因分子标记检测[J]. 北方园艺, 2024(9):24-32.
本文引用 [1]
Share on Mendeley
PDF(3708 KB)

59

Accesses

0

Citation

Detail
Sections
Recommended

/

〈 〉