Soybean Germplasm Resources at Germination: Salt Tolerance Evaluation and Mechanism Analysis

WANG Meng, LIU Wenjun, LU Xueli, CHEN Qingshan, YANG Mingliang, LV Bo, XU Zongchang

PDF(1812 KB)
PDF(1812 KB)
Chinese Agricultural Science Bulletin ›› 2023, Vol. 39 ›› Issue (26) : 8-16. DOI: 10.11924/j.issn.1000-6850.casb2022-0834

Soybean Germplasm Resources at Germination: Salt Tolerance Evaluation and Mechanism Analysis

Author information +
History +

Abstract

In order to select salt-tolerant soybean germplasm as the foundation for salt-tolerance breeding, 52 soybean genotypes were exposed to 0.4% of NaCl and 0.8% of NaCl, and the germination indices and some seedling stage indices were investigated for salt tolerance. The results showed that the germination potential, germination rate, hypocotyl length and diameter of the population were decreased significantly under 0.8% of NaCl treatment, compared with the control and 0.4% of NaCl treatment, but there were significant differences among individuals in the population. Salt-intolerant soybean germplasm had significantly lower chlorophyll content and leaf relative water content under 0.4% and 0.8% of NaCl stress than salt-tolerant soybean germplasm. The ability of holding Na+ in the roots in salt-intolerant germplasm was decreased under 0.8% of NaCl stress, while Na+ contents in the stems were higher than that of salt-tolerant germplasm. A total of 18 salt-tolerant soybean germplasm materials, 23 intermediate soybean germplasm materials and 11 salt-intolerant soybean germplasm materials were screened by membership function method.

Key words

soybean / salt resistance / germplasm resources / germination period / membership function method

Cite this article

Download Citations
WANG Meng , LIU Wenjun , LU Xueli , CHEN Qingshan , YANG Mingliang , LV Bo , XU Zongchang. Soybean Germplasm Resources at Germination: Salt Tolerance Evaluation and Mechanism Analysis. Chinese Agricultural Science Bulletin. 2023, 39(26): 8-16 https://doi.org/10.11924/j.issn.1000-6850.casb2022-0834

References

[1]
PITMAN M G, LÄUCHLI A. Global impact of salinity and agricultural ecosystems In: Läuchli A., Lüttge U. (eds) Salinity: Environment-plants-molecules[M]. Springer, dordrecht, 2002:3-20.
[2]
赵秀芳, 宋国香, 谢志远, 等. 中国盐碱土修复现状与特点[J]. 环境卫生工程, 2018, 35(4):96-99.
[3]
王佺珍, 刘倩, 高娅妮, 等. 植物对盐碱胁迫的响应机制研究进展[J]. 生态学报, 2017, 37(16):5565-5577.
[4]
张春兰, 曹帅, 满丽莉, 等. 不同基因型大豆耐盐性筛选与综合鉴定[J]. 内蒙古民族大学学报(自然科学版), 2019, 34(4):342-349.
[5]
韩毅强, 高亚梅, 杜艳丽, 等. 大豆耐盐碱种质资源鉴定[J]. 中国油料作物学报, 2021, 43(6):1016-1024.
[6]
张军起, 赵霞, 张豪, 等. 不同大豆种子萌发期耐盐性分析[J]. 山西农业科学, 2019, 47(5):770-774,779.
[7]
邵桂花, 宋景芝, 刘惠令. 大豆种质资源耐盐性田间鉴定方法[J]. 作物杂志, 1986, 20(3):36-37.
[8]
张彦威, 刘国峰, 李伟, 等. 黄淮海地区大豆种质资源耐盐性鉴定[J]. 山东农业科学, 2018, 50(11):33-36.
[9]
姜奇彦, 胡正, 张辉, 等大豆种质资源耐盐性鉴定与研究[J]. 植物遗传资源学报, 2012, 13(5):726-732.
[10]
刘谢香, 常汝镇, 关荣霞, 等. 大豆出苗期耐盐性鉴定方法建立及耐盐种质筛选[J]. 作物学报, 2020, 46(1):1-8.
[11]
姬丹丹, 刘畅, 曹其聪, 等. 不同大豆品种芽期和苗期耐盐性比较研究[J]. 山东轻工业学院学报, 2011, 25(2):4-7.
[12]
邹平. 特定乙酰度壳寡糖诱导小麦抗盐作用及其机理研究[D]. 青岛: 中国科学院研究生院-海洋研究所, 2015.
[13]
WANG M, REN T, HUANG R, et al. Overexpression of an Apocynum venetum flavonols synthetase gene confers salinity stress tolerance to transgenic tobacco plants[J]. Plant physiology and biochemistry, 2021, 162:667-676.
Soil salinity is a major limiting factor for agricultural production, threatening food security worldwide. A thorough understanding of the mechanisms underlying plant responses is required to effectively counter its deleterious effects on crop productivity. Total flavonoid accumulation reportedly improves salinity tolerance in many crops. Therefore, we isolated the full-length cDNA of a flavonol synthetase (FLS) gene from Apocynum venetum (AvFLS). The gene contained a 1008-bp open reading frame encoding a protein composed of 335 amino acid residues. Multiple sequence alignment showed that the AvFLS protein was highly homologous to FLSs from other plants. AvFLS was expressed in leaves, stems, roots, flowers, and germinated seeds. Expression pattern analysis revealed that AvFLS was significantly induced by salinity stress. AvFLS overexpression in tobacco positively affected the development and growth of transgenic plants under salinity stress: root and seedling growth were inhibited to a lesser extent, while seed germination rate increased. Additionally, the overexpression of AvFLS under salinity stress resulted in an increase in total flavonoid content (1.63 mg g in wild-type samples and 4.63 mg g on average in transgenic samples), which accompanied the increase in the activity of antioxidant enzymes and inhibited the production of reactive oxygen species. Further, AvFLS-overexpressing transgenic tobacco plants absorbed more K than wild type plants, leading to an increased K/Na ratio, which in turn contributed to the maintenance of Na/K homeostasis. These findings suggest that an AvFLS-induced increase in total flavonoid content enhanced plant salinity tolerance, implying the importance of AvFLS gene responses to salinity stress.Copyright © 2021 Elsevier Masson SAS. All rights reserved.
[14]
李玲, 沈宝宇, 张天静, 等. 豌豆种质资源芽期耐旱性评价及耐旱种质筛选[J]. 植物遗传资源学报, 2017, 18(4):778-785.
试验采用PEG-6000作为渗透介质,研究了不同浓度PEG-6000旱胁迫下4份豌豆种质的发芽势、发芽率、发芽指数及简易活力指数四个指标的变化动态,确定了豌豆芽期耐旱性鉴定适宜PEG-6000浓度为20%-25%。进而以22%PEG-6000模拟旱胁迫对来自我国18个不同省份的87份豌豆种质资源进行耐旱性鉴定,主成分分析确定了相对发芽势、相对发芽率等7项指标作为耐旱综合评价因子,利用隶属函数法,筛选出1份高抗资源(G0002457,白豌豆),7份抗旱种质。为豌豆耐旱品种改良奠定基础.
[15]
张彦威, 张礼凤, 李伟, 等. 大豆发芽期和苗期耐盐性的隶属函数分析[J]. 山东农业科学, 2016, 48(1):21-25.
[16]
PARKER M B, GAINES T P, HOOK J E, et al. Chloride and water stress effects on soybean in pot culture[J]. Journal of plant nutrition, 2008, 10(5):517-538
[17]
WEIL R, KHALIL N. Salinity tolerance of winged beans as compared to that of soybean[J]. Agronomy journal, 1985, 78:67-70.
Winged bean [Psophocarpus tetragonolobus(L.) DC] has been called an underutilized crop with the potential to become a major protein crop for the tropics and subtropics. As with soybean [Glycine max(L.) Merr.] the introduction of winged bean to warm arid regions will be affected by tolerance to salinity. In this study a prolific Sri Lankan winged bean accession (‘SLS47’) and two soybean cultivars (‘Jackson’ and ‘Lee’) were grown in pots of sandy soil amended with NaCL to give saturated paste electrical conductivities (EC) of 0.5, 2.5, 4.5, 6.5 and 8.5 dS m−1. At 0.5 dS m−1, dry matter accumulation (0.84 g plant−1), nodule mass, (32 mg plant−1), and tissue N (24 mg Ng−1) of winged bean were similar to those of both soybean cultivars. At 65 d after planting, however, specific nitrogenase activity by acetylene reduction assay was much lower for the winged bean (60 μmol ethylene g−1h−1) than for the soybean (160 to 190 μmol ethylene g−1h−1). The results indicated that winged bean was at least as tolerant as Lee soybean and more tolerant than Jackson. Though stunted, at 8.5 dS m−1winged bean showed significantly less foliar injury than did either of the soybean cultivars. For the soybean, the tissue N content was unchanged, but for winged bean it increased significantly with increasing salinity, suggesting that dry matter accumulation,especially by winged bean, was more sensitive to salinity than to N2fixation.
[18]
ZHANG W, NIU Y, BU S, et al. Epistatic association mapping for alkaline and salinity tolerance traits in the soybean germination stage[J]. Plos one, 2014, 9(1):e84750
[19]
张新草, 薛项潇, 姜深, 等. 大豆种质发芽期耐盐碱性鉴定及指标筛选[J]. 西北农业学报, 2020, 29(3):374-381.
[20]
石广成, 杨万明, 杜维俊, 等. 大豆耐盐种质的筛选及其耐盐生理特性分析[J]. 2022, 38(4):41-50.
[21]
罗庆云. 野生大豆和栽培大豆耐盐机理及遗传研究[D]. 南京: 南京农业大学, 2003.
[22]
DO T, LAL S K, XU D H. Identification of a major QTL allele from wild soybean (Glycine soja Sieb. & Zucc.) for increasing alkaline salt tolerance in soybean[J]. Theoretical and applied genetics, 2010, 121(2):229-236.
[23]
刘奕媺, 于洋, 方军. 盐碱胁迫及植物耐盐碱分子机制研究[J]. 土壤与作物, 2018, 7(2):201-211.
[24]
杨劲松, 姚荣江, 王相平, 等. 中国盐渍土研究:历程现状与展望[J]. 土壤学报, 2022, 50(1):10-27.
[25]
陈晓亚, 汤章城. 植物生理与分子生物学(3版)[M]. 北京: 高等教育出版社, 2007.
[26]
罗庆云, 於丙军, 刘友良. NaCl胁迫下Cl-和Na对大豆幼苗胁迫作用的比较[J]. 中国农业科学, 2003, 36(11):1390-1394.
[27]
刘光宇, 关荣霞, 常汝镇, 等. 大豆不同器官Na+含量与苗期耐盐性的相关分析[J]. 作物学报, 2011, 37(7):1266-1273.
Share on Mendeley
PDF(1812 KB)

Accesses

Citation

Detail

Sections
Recommended

/