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Effects of Drought Stress and Rehydration on Physiological Characteristics of Hemerocallis fulva
WANG Yang, YU Senmiao, WANG Jingyang, SONG Hailong, LEI Yaodong, ZHANG Haiyan, LIU Bo
PDF(1340 KB)
PDF(1340 KB)
Effects of Drought Stress and Rehydration on Physiological Characteristics of Hemerocallis fulva
To study the effects of drought stress and rehydration on the physiological characteristics of day lily (Hemerocallis fulva), the changes of relative water content, chlorophyll, relative electrical conductivity, antioxidant enzyme activity and osmotic regulation substances in leaves were measured by water control in pot culture to simulate drought and rewatering. The results showed that: (1) with the progress of drought stress, the leaf relative water content in mild, moderate and severe drought treatments decreased gradually, and recovered after rewatering, but still significantly lower than that of the control treatment (P<0.05). (2) The chlorophyll content increased at first and then decreased, and increased after rewatering. The chlorophyll content of mild and moderate drought stress treatments was not significantly different from that of the control group (P>0.05). (3) The relative conductivity was significantly higher than that of the control group (P<0.05) until 28 days, and the relative conductivity of each treatment group decreased significantly after rehydration. With the extension of stress time, the content of MDA in mild, moderate and severe drought treatments increased gradually, and decreased rapidly after rewatering, but it was significantly higher than that in the control group (P<0.05). (4) Soluble sugar and proline increased continuously in the three treatment groups. After rehydration, the contents of soluble sugar and proline in all treatment groups decreased significantly, but they were still significantly higher than those in the control group (P<0.05). (5) The activities of SOD, POD and CAT in each treatment group increased in varying degrees. After rehydration, the activities of POD, SOD and CAT in mild, moderate and severe drought treatments decreased, but still higher than the normal level.
Hemerocallis fulva / drought stress / rehydration / osmoregulation / antioxidase {{custom_keyword}} /
| [1] |
段娜, 王佳, 刘芳, 等. 植物抗旱性研究进展[J]. 分子植物育种, 2018, 16(15):5093-5099.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [2] |
张金政, 张起源, 孙国峰, 等. 干旱胁迫及复水对玉簪生长和光合作用的影响[J]. 草业学报, 2014, 23(1):167-176.
以东北玉簪和紫萼玉簪为材料,研究了干旱胁迫及复水对玉簪干物质量、部分形态指标、叶绿素含量、气体交换和叶绿素荧光参数的影响,结果表明,随干旱胁迫时间的延长和干旱程度的增加,干旱胁迫处理对2种玉簪干物质量、部分形态指标、叶绿素含量的抑制作用逐渐增大。此外,干旱胁迫处理能够降低或显著降低2种玉簪的净光合速率(P<sub>n</sub>)、气孔导度(G<sub>s</sub>)、蒸腾速率(T<sub>r</sub>),降低或升高胞间CO<sub>2</sub>浓度(C<sub>i</sub>);降低或显著降低PSII最大光能转化效率(F<sub>v</sub>/F<sub>m</sub>)、光适应下PSII最大光能转化效率(F<sub>v</sub>'/F<sub>m</sub>')、PSII电子传递量子效率(Φ<sub>PSII</sub>)和光化学猝灭系数(qP),显著提高非光化学猝灭系数(NPQ)。而干旱胁迫处理15或30 d后复水15 d,各指标均能得到不同程度的恢复;30 d的干旱胁迫未造成光合细胞器的不可逆损伤。2种玉簪相关生理指标的比较分析结果显示,东北玉簪抗旱能力强于紫萼玉簪。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [3] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [4] |
陈爱萍, 隋晓青, 王玉祥, 等. 干旱胁迫及复水对伊犁绢蒿幼苗生长及生理特性的影响[J]. 草地学报, 2020, 28(5):1216-1225.
为研究伊犁绢蒿（Seriphidium transiliense）幼苗对干旱胁迫的适应能力，本研究采用盆栽控水法分析了持续干旱胁迫及复水过程伊犁绢蒿幼苗生长及生理指标的变化。结果表明：随着干旱胁迫的加剧，幼苗地上部分生长逐渐减慢，重度干旱胁迫下幼苗株高和地上生物量较对照分别降低了50.29%和50.82%（PP<0.05）；叶片相对含水量、叶绿素含量和根系活力持续下降；叶片和根系的相对电导率、丙二醛含量、可溶性糖和脯氨酸含量、超氧化物歧化酶和过氧化物酶活性逐渐升高，而可溶性蛋白质含量和过氧化氢酶活性则呈先升高后降低的趋势。综上所述，伊犁绢蒿幼苗可以通过调节生长和生物量的分配，并通过积累渗透调节物质和提高抗氧化酶活性来清除活性氧，维持其在干旱胁迫下的生长；复水后，叶片和根系的渗透调节物质及抗氧化酶活性大多能恢复至中度干旱胁迫水平，表明伊犁绢蒿幼苗具有较强的干旱适应能力。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [5] |
冯树林. 干旱胁迫和复水对侧柏和紫穗槐幼苗生长和生理特征的影响研究[D]. 杨凌: 西北农林科技大学, 2020.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [6] |
Proline (Pro) accumulation is a common physiological response in many plants in response to a wide range of biotic and abiotic stresses. Controversy has surrounded the possible role(s) of proline accumulation. In this review, knowledge on the regulation of Pro metabolism during development and stress, results of genetic manipulation of Pro metabolism and current debate on Pro toxicity in plants are presented.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [7] |
曲艳, 李青丰, 窦焱艳, 等. 射干种子萌发特性及对干旱胁迫的响应[J]. 种子, 2019, 38(10):24-28,33.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [8] |
陈丽飞, 董然, 顾德峰, 等. 大花萱草对水分胁迫的光合响应[J]. 北方园艺, 2011(19):61-63.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [9] |
陈羡德, 陈珺, 付佩琦, 等. 干旱胁迫对不同品种大花萱草光合特性的影响[J]. 上海交通大学学报(农业科学版), 2019, 37(5):6-9.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [10] |
谭春燕, 杨文钰, 陈佳琴, 等. 干旱胁迫下大豆种质资源的生理响应及抗旱性评价[J]. 分子植物育种, 2020, 18(4):1349-1356.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [11] |
郑锦秀, 姜辉, 王永翠, 等. 陆地棉亚鸡脚叶种质耐干旱胁迫分析[J]. 分子植物育种, 2020, 18(24):8273-8279.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [12] |
陈爱葵, 韩瑞宏, 李东洋, 等. 植物叶片相对电导率测定方法比较研究[J]. 广东教育学院学报, 2010, 30(5):88-91.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [13] |
李仕飞, 刘世同, 周建平, 等. 分光光度法测定植物过氧化氢酶活性的研究[J]. 安徽农学通报, 2007(2):72-73.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [14] |
王朝英, 方文, 陈丽花, 等. 红叶加拿大紫荆对不同强度干旱胁迫的生长及生理响应[J]. 北方园艺, 2020(20):62-69.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [15] |
孙晓春, 黄文静, 李铂, 等. 外源水杨酸对干旱胁迫下桔梗幼苗生理生化指标的影响[J]. 北方园艺, 2019(16):121-125.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [16] |
谭占明, 张朋朋, 吴翠云, 等. 干旱胁迫对两个杏品种的生长和生理指标的影响[J]. 北方园艺, 2020(8):50-54.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [17] |
Shoots, roots, and seeds of corn (Zea mays L., cv. Michigan 500), oats (Avena sativa L., cv. Au Sable), and peas (Pisum sativum L., cv. Wando) were analyzed for their superoxide dismutase content using a photochemical assay system consisting of methionine, riboflavin, and p-nitro blue tetrazolium. The enzyme is present in the shoots, roots, and seeds of the three species. On a dry weight basis, shoots contain more enzyme than roots. In seeds, the enzyme is present in both the embryo and the storage tissue. Electrophoresis indicated a total of 10 distinct forms of the enzyme. Corn contained seven of these forms and oats three. Peas contained one of the corn and two of the oat enzymes. Nine of the enzyme activities were eliminated with cyanide treatment suggesting that they may be cupro-zinc enzymes, whereas one was cyanide-resistant and may be a manganese enzyme. Some of the leaf superoxide dismutases were found primarily in mitochondria or chloroplasts. Peroxidases at high concentrations interfere with the assay. In test tube assays of crude extracts from seedlings, the interference was negligible. On gels, however, peroxidases may account for two of the 10 superoxide dismutase forms.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [18] |
李青, 秦玉芝, 王万兴, 等. 马铃薯(Solanum tuberosum L.)干旱胁迫生理特性及耐旱性分析[J]. 分子植物育种, 2021, 19(1):259-268.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [19] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [20] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [21] |
王宁, 袁美丽, 陈浩, 等. 干旱胁迫及复水对入侵植物节节麦幼苗生长及生理特性的影响[J]. 草业学报, 2019, 28(1):70-78.
以节节麦幼苗为试材,采用盆栽称重控水法,进行了28 d干旱及7 d复水处理,研究干旱胁迫及复水对节节麦幼苗叶、根生物量比等形态参数、保护酶活性等生理参数的影响。旨在探讨节节麦形态和生理特征可塑性对土壤水分变化的响应特性,为其耐旱机制研究提供参考依据。结果表明:1)干旱胁迫过程中,叶片相对含水量、相对电导率、丙二醛和脯氨酸含量均呈持续增加的变化趋势;叶绿素含量呈先升后降变化趋势;轻度及中度干旱胁迫下超氧化物岐化酶(SOD)活性持续增加,而重度干旱胁迫中则呈先升后降的变化趋势;2)干旱胁迫致使节节麦幼苗叶生物量比、比叶面积下降,根生物量比及根冠比增加;3)复水后,叶片相对含水量、脯氨酸等多数指标均能迅速恢复至正常水平,表明节节麦对干旱胁迫造成的损伤具有较高的修复能力。节节麦通过一系列形态及生理特征的可塑性变化适应土壤干旱生境,从而表现出较强的耐旱性。此外,生理参数可塑性指数的均值明显大于形态参数,说明节节麦主要通过调整生理代谢以适应短期干旱胁迫。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [22] |
刘新, 符开欣, 郭志慧, 等. 6份川西北短芒披碱草种质苗期的抗旱性综合评价[J]. 草业科学, 2017, 34(1):101-111.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [23] |
谢贤健, 兰代萍, 白景文. 三种野生岩生草本植物的抗旱性综合评价[J]. 草业学报, 2009, 18(4):75-80.
本试验选择自然状态下生长在岩石边坡上的3种野生岩生植物金发草和丛毛羊胡子草以及狗牙根为研究材料,通过盆栽干旱胁迫试验,研究和分析了干旱胁迫下3种野生岩生植物的叶面积指数、地下干物质量积累、叶片保水力;叶片相对电导率、可溶性蛋白、叶绿素等形态和生理指标,利用主成分分析法和隶属函数值法对影响3种野生岩生植物抗旱性的指标进行了分析评价。试验结果表明,3种草的抗旱性强弱顺序为金发草＞狗牙根＞丛毛羊胡子草。研究结果以期为筛选、培育适应于岩石边坡植被防护的乡土护坡植物的合理开发、利用提供理论依据。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [24] |
李倩, 杜勇军. 水分对大花萱草生理特性的影响[J]. 分子植物育种, 2018, 16(21):7219-7224.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [25] |
周欢欢, 傅卢成, 马玲, 等. 干旱胁迫及复水对‘波叶金桂'生理特性的影响[J]. 浙江农林大学学报, 2019, 36(4):687-696.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [26] |
李明, 王根轩. 干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J]. 生态学报, 2002(4):503-507.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [27] |
牛东伟, 马男, 房卓研, 等. 水分胁迫下墨西哥羽毛草耐旱生理[J]. 草业科学, 2018, 35(3):581-589.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [28] |
黄桂华, 梁坤南, 周再知, 等. 柚木无性系苗期抗旱生理评价与选择的研究[J]. 中南林业科技大学学报, 2018, 38(5):11-17.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [29] |
Proline accumulates in many plant species in response to environmental stress. Although much is now known about proline metabolism, some aspects of its biological functions are still unclear. Here, we discuss the compartmentalization of proline biosynthesis, accumulation and degradation in the cytosol, chloroplast and mitochondria. We also describe the role of proline in cellular homeostasis, including redox balance and energy status. Proline can act as a signaling molecule to modulate mitochondrial functions, influence cell proliferation or cell death and trigger specific gene expression, which can be essential for plant recovery from stress. Although the regulation and function of proline accumulation are not yet completely understood, the engineering of proline metabolism could lead to new opportunities to improve plant tolerance of environmental stresses.Copyright 2009 Elsevier Ltd. All rights reserved.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [30] |
施钦, 包学文, 华建峰, 等. 干旱胁迫及复水对海滨木槿光合作用和生理特性的影响[J]. 应用生态学报, 2019, 30(8):2600-2606.
选择2年生海滨木槿扦插苗为材料,通过自然干旱20 d而后复水(21 d),研究了其在干旱和复水过程中的光合作用和生理特性.结果表明: 经过20 d的干旱胁迫,在土壤含水量仅为5.9%的情况下海滨木槿全部成活.干旱胁迫显著降低了海滨木槿的净光合速率,实测值最高仅为1.1 μmol·m<sup>-2</sup>·s<sup>-1</sup>,最大光化学效率为对照的84.3%.可溶性蛋白和抗氧化酶同时积累,稳定细胞渗透势并清除干旱造成的脂膜过氧化物质.复水7 d后光合速率升高至对照的57.3%,超氧化物歧化酶和过氧化氢酶活性随着丙二醛含量下降而下降.复水21 d后,海滨木槿抗氧化酶、可溶性蛋白和叶片相对含水量均恢复至对照水平,干旱处理总生物量虽显著降低,但根冠比显著提高.海滨木槿具有极强的耐旱能力,是适用于沿海地区景观改良和道路绿化的重要树种.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [31] |
陈敏菊, 孟彦, 孟宪政. 干旱胁迫对甜高粱幼苗光合色素、保护酶活性及活性氧代谢的影响[J]. 天津农业科学, 2021, 27(9):1-4.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [32] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [33] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [34] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [35] |
赵英, 吴敏, 邓平. 干旱与复水对2种蟛蜞菊生长及生理生化特性的影响[J]. 西北农林科技大学学报(自然科学版), 2021, 49(4):113-122.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| {{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
PDF(1340 KB)
图1 干旱胁迫及复水处理对叶片相对含水量的影响图2 干旱胁迫及复水处理对叶绿素含量的影响图3 干旱胁迫及复水处理对叶片相对电导率的影响图4 干旱胁迫及复水处理对MDA含量的影响图5 干旱胁迫及复水处理对脯氨酸含量的影响图6 干旱胁迫及复水处理对可溶性糖含量的影响图7 干旱胁迫及复水处理对SOD活性的影响图8 干旱胁迫及复水处理对POD活性的影响图9 干旱胁迫及复水处理对CAT活性的影响/
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