Qingyazhai National Nature Reserve has rich forest resources and wild plant resources, which is a hotspot area of biodiversity research. The spatial pattern analysis of Qingyazhai National Nature Reserve has important value and significance for the protection of biodiversity at the regional scale. The geographical and natural conditions of Qingyazhai National Nature Reserve were analyzed by using the forest resource survey data in 2003, 2016 and 2020, and ArcGIS10.7 software, so as to obtain topographic factors, stand survey factors, spatial distribution pattern of key protected wild plants and suitable habitat distribution. Through overlapping analysis of the suitable habitat distribution and the spatial distribution of the richness of key protected wild plants, the hotspot areas of key protected wild plants were determined. The results show that Qingyazhai National Nature Reserve has a large area of forest land, mainly young age groups, and high plant diversity in the range of middle canopy density (0.4-0.6). There are 31 species of key protected wild plants in the reserve, including 5 species of national level II wild protected plants. There are two hotspot areas in the reserve, one is located in the northwest of the reserve, which is mainly distributed in Changshou village, Huangzhuang, Lianggou and Chaoyanggou; the other is in the central part of the reserve, located at the junction of Naogou, Houqu, Qugou, Qibugou and Muzuo village. The spatial pattern analysis provides a scientific basis for the study of plant resource richness and diversity in nature reserves, and also provides planning reference for the construction of key protected areas of wild plants in nature reserves.