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Change Characteristics of Microbial Community in the Rhizosphere of Papaya Under Papaya-Leek Intercropping
WANG Lixia, YIN Xiaomin, LIU Yongxia, LIAN Zihao, WANG Bizun, HE Yingdui
PDF(2996 KB)
PDF(2996 KB)
Change Characteristics of Microbial Community in the Rhizosphere of Papaya Under Papaya-Leek Intercropping
The variation characteristics of microbial community in rhizosphere of papaya under papaya-leek intercropping pattern were studied by using volatile substances released in leek habitat to inhibit the pathogenic fungi in the rhizosphere of papaya. Soil microbial DNA was extracted at different intercropping periods, and soil microbial diversity in rhizosphere was analyzed by Illumina Miseq high-throughput sequencing technology. The results showed that: (1) the soil bacteria in the rhizosphere were classified into 27 phyla, 64 classes, 146 orders, 272 families and 437 genera, Acidobacteria, Actinobacteria, Chloroflexi and Proteobacteria were the dominant bacteria, and their proportions were 18.15%, 22.33%, 19.11%, and 25.53%, respectively, accounting for 85.12% of the total bacterial composition; with the increase of intercropping time, the advantages of Proteobacteria and Acidobacteria were obvious; (2) the soil fungi of rhizosphere were classified into 7 phyla, 24 classes, 64 orders, 126 families and 239 genera, Ascomycota, Zygomycota and Basidiomycota were the dominant, and their proportions were 93.71%, 3.94% and 1.18%, respectively, accounting for 98.83% of the total composition; and Ascomycota was the dominant; (3) at the genus level of bacteria, the relative abundance of Subgroup _6_ norank of Acidobacteria was the highest at 30, 60 and 120 d of intercropping; at 90 d, the relative abundance of Nitrospira was the highest; at the genus level of fungi, the relative abundance of the genus of Ascomycota was the highest whether papaya was intercropped with leek or not; (4) soil organic matter, soil total nitrogen and soil available phosphorus in the rhizosphere were the key factors affecting the soil microbial community structure and soil microbial diversity in papaya-leek intercropping pattern.
papaya / papaya-leek intercropping / soil nutrients / soil microorganism {{custom_keyword}} /
表1 间作韭菜模式下番木瓜根区土壤细菌物种多样性相关指数 |
| 时间/d | OTU数量/个 | 覆盖率 | ACE指数 | Chao指数 | Shannon-Wiener指数 | Simpson指数 |
|---|---|---|---|---|---|---|
| 0 | 1600.00±20.00a | 0.9873±0.0017a | 1822.00±51.07a | 1812.67±63.37a | 5.6800±0.0360a | 0.0385±0.0019a |
| 30 | 1667.00±43.00b | 0.9884±0.0010a | 1848.33±44.41ab | 1857.67±60.47ab | 6.3700±0.0436c | 0.0039±0.0001b |
| 60 | 1610.00±6.00a | 0.9876±0.0014a | 1826.33±49.66a | 1823.65±65.05a | 6.2600±0.0265b | 0.0045±0.0002b |
| 90 | 1760.00±30.00c | 0.9883±0.0007a | 1930.00±41.90b | 1958.67±62.85b | 6.5200±0.0173d | 0.0032±0.0001b |
| 120 | 1718.00±10.00c | 0.9889±0.0010a | 1876.67±39.72ab | 1892.46±55.56ab | 6.4800±0.0200d | 0.0033±0.0002b |
| 注：各相关指数相似性水平为97%,同列数据后不同小写字母表示差异显著(P<0.05),下同。 |
表2 间作韭菜模式下番木瓜根区土壤真菌物种多样性相关指数 |
| 时间/d | OTU数量/个 | 覆盖率 | ACE指数 | Chao指数 | Shannon-Wiener指数 | Simpson指数 |
|---|---|---|---|---|---|---|
| 0 | 239.00±9.54a | 0.9981±0.0003a | 290.00±21.00a | 284.00±12.12a | 1.8033±0.0379a | 0.4119±0.0063a |
| 30 | 348.00±10.00d | 0.9986±0.0005a | 373.00±6.56bc | 381.67±19.01bc | 3.4867±0.0217e | 0.0741±0.0014b |
| 60 | 321.00±11.00c | 0.9982±0.0004a | 361.33±12.50b | 362.33±10.60b | 3.2233±0.0153d | 0.0894±0.0015c |
| 90 | 320.00±5.00c | 0.9975±0.0016a | 392.00±16.00c | 403.00±14.11c | 2.8200±0.0265c | 0.1491±0.0026d |
| 120 | 300.00±12.00b | 0.9973±0.0010a | 381.00±13.53bc | 388.33±10.21c | 2.0367±0.0252b | 0.3799±0.0062e |
表3 各采样时间土壤理化性质变化 |
| 采样时间 | 有机质/(g/kg) | pH | 全氮/(g/kg) | 速效磷/(mg/kg) | 速效钾/(mg/kg) |
|---|---|---|---|---|---|
| PL-0 | 23.93±0.06b | 6.09±0.08ab | 0.97±0.06a | 14.80±0.20a | 820.00±43.59a |
| PL-30 | 22.97±0.06a | 5.92±0.18a | 0.95±0.01a | 22.33±0.25b | 1340.00±20.00d |
| PL-60 | 23.00±0.00a | 6.13±0.06b | 0.92±0.06b | 35.07±0.25d | 1233.33±11.55c |
| PL-90 | 23.93±0.06b | 6.63±0.06c | 1.04±0.02c | 29.70±0.00c | 1016.67±5.77b |
| PL-120 | 25.87±0.06c | 6.93±0.06d | 1.03±0.01c | 45.70±0.36e | 826.67±5.77a |
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PDF(2996 KB)
Collection(s)
表1 间作韭菜模式下番木瓜根区土壤细菌物种多样性相关指数表2 间作韭菜模式下番木瓜根区土壤真菌物种多样性相关指数
图1 间作韭菜后番木瓜根区细菌门优势类群相对丰度图2 间作韭菜后番木瓜根区细菌属优势类群相对丰度图3 间作韭菜后番木瓜根区细菌群落结构热图图4 间作韭菜后番木瓜根区真菌门水平优势类群的相对丰度图5 间作韭菜后番木瓜根区真菌属水平优势类群的相对丰度图6 间作韭菜后番木瓜根区真菌群落结构热图表3 各采样时间土壤理化性质变化图7 土壤细菌群落结构与环境因子的相关性图8 土壤真菌群落结构与环境因子的相关性/
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