Study on Germination Characteristics and Dormancy Breaking Methods of Tilia amurensis Seeds

WANG Yinhua, KONG Yuguang, LI Qinghua, WU Dejun, YAN Liping, XU Tao, LU Yizeng, ZHAI Guofeng

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Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (29) : 80-85. DOI: 10.11924/j.issn.1000-6850.casb2021-1015

Study on Germination Characteristics and Dormancy Breaking Methods of Tilia amurensis Seeds

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Abstract

In order to find out the cause of Tilia amurensis seed dormancy and a method to quickly relieve the dormancy, the seed coat permeability, seed germination characteristics and dormancy release methods of Tilia amurensis pure seeds were studied. The permeability test results showed that: when treated with concentrated H2SO4, the permeability and water absorption of Tilia amurensis seeds increased significantly. The experiment of seed germination characteristics showed that the complete seed germination rate of Tilia amurensis was 0. After removing the seed coat, the germination rate of seeds with intact endosperm, partially removed endosperm (root tip was not exposed), cap-stripped endosperm with exposed root tip, and in vitro embryos was 4.0%, 23.3%, 84.7%, and 100%, respectively. The results show that Tilia amurensis seeds have dormancy, but their seed embryos do not have dormancy. The dormancy is caused by extraembryonic factors, and endosperm (especially endosperm cap) is the main cause of seed dormancy of Tilia amurensis. The test results of seed dormancy breaking method show that the dormancy of Tilia amurensis seeds can be quickly relieved by being treated with concentrated H2SO4 for 15 min + soaking in 500 mg/L GA3 solution for 12 h + at low temperature stratification for 30 d, reaching a germination rate of 92.0%. In conclusion, the seed coat of Tilia amurensis seeds has a certain permeability barrier, but the endosperm cap is the main reason for its dormancy. The comprehensive treatment method of concentrated H2SO4 treatment + GA3 soaking + low temperature stratification can relieve the dormancy of Tilia amurensis seeds within 30 days.

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Tilia amurensis / dormancy / germination characteristics / dormancy breaking / low temperature stratification

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WANG Yinhua , KONG Yuguang , LI Qinghua , WU Dejun , YAN Liping , XU Tao , LU Yizeng , ZHAI Guofeng. Study on Germination Characteristics and Dormancy Breaking Methods of Tilia amurensis Seeds. Chinese Agricultural Science Bulletin. 2022, 38(29): 80-85 https://doi.org/10.11924/j.issn.1000-6850.casb2021-1015

References

Publishing order | Descend order by publishing year | Descend order by cited within
[1]
国家林业局, 农业部. 国家重点保护野生植物名录(第一批)[M]. 北京: 农业部,1999.
本文引用 [1]
[2]
朱红波, 林士杰, 张忠辉, 等. 椴树属树种种子休眠原因及提高种子萌发率概述[J]. 中国农学通报, 2011, 27(22):1-4.
本文引用 [1]
[3]
邹学忠, 阎忠林, 刘清田, 等. 紫椴种子休眠原因及催芽处理方法的研究[J]. 辽宁林业科技, 1991(5):6-10.
本文引用 [1]
[4]
王海南. 紫椴种子休眠机理及休眠解除技术的研究[D]. 哈尔滨: 东北林业大学, 2012.
本文引用 [1]
[5]
刘艳秋. 紫椴种子休眠原因的初步研究[J]. 科技信息, 2010(34):409,411.
本文引用 [1]
[6]
尹黎燕, 王彩云, 叶要妹, 等. 观赏树木休眠的研究方法[J]. 种子, 2002(1):4547.
本文引用 [1]
[7]
杜凤国, 苏春华, 梨云风, 等. 紫椴和糠椴种子解剖构造的研究[J]. 吉林林学院学报, 1994, 10(2):99-104.
本文引用 [2]
[8]
史锋厚, 朱灿灿, 沈永宝, 等. 南京椴种子的萌发与休眠[J]. 福建林学院学报, 2008(1):48-51.
本文引用 [2]
[9]
有祥亮. 欧洲栓皮槭和银叶椴引种的适应性、繁殖技术与经济价值研究[D]. 北京: 北京林业大学, 2006.
本文引用 [1]
[10]
王九龄, 杨建平. 紫椴种子休眠原因的初步研究[J]. 林业科学, 1981(3):317-324.
本文引用 [2]
[11]
魏灵敏, 马玉杰, 李佛莲, 等. 不同处理对拐枣种子发芽的影响[J]. 西部林业科学, 2017, 46(4):67-72.
本文引用 [1]
[12]
韩月乔, 于营, 王志清, 等. 白藓种子休眠原因及解除方法研究[J]. 河北农业大学学报, 2015, 38(2):43-47.
本文引用 [2]
[13]
符近, 尤瑞麟, 顾增辉. 马占相思种子休眠的研究[J]. 北京大学学报(自然科学版), 1997(6):79-85.
本文引用 [1]
[14]
贾有伟, 魏驰宇, 田如男. 欧洲冬青种皮特性与种子休眠的关系[J]. 江苏农业科学, 2017, 45(12):96-99.
本文引用 [1]
[15]
周健, 代松, 钱存梦, 等. 大花四照花种子休眠解除过程中种皮和胚乳结构变化[J]. 东北林业大学学报, 2016, 44(5):34-38.
本文引用 [1]
[16]
卢明艳, 杜研, 毕晓颖. 5种野生无髯鸢尾种子休眠与萌发特性研究[J]. 西北植物学报, 2017, 37(9):1823-1830.
本文引用 [1]
[17]
李成浩, 牛遇达, 刘桂丰, 等. 胚乳和外源赤霉素对五味子[Schisandra chinensis (Turcz.) Baill]种子发芽的影响[J]. 植物生理学通讯, 2006(4):625-627.
本文引用 [1]
[18]
J DEREK BEWLEY, 任成伟, 鄢波. 种子的发芽和休眠(Ⅰ)[J]. 种子, 2002(6):84-86.
本文引用 [1]
[19]
张丹, 张金政, 孙国峰, 等. 细叶鸢尾种子休眠原因及解除休眠的方法研究[J]. 草业学报, 2011, 20(6):109-117.
本文引用 [1]
[20]
王宏迪, 程秋香, 李吟平, 等. 细胞壁降解酶与黄精种子胚乳弱化的关系[J]. 种子, 2018, 37(7):23-27.
本文引用 [1]
[21]
张云, 马玉梅, 秦景逸, 等. 膜苞鸢尾种子休眠及萌发特性研究[J]. 草地学报, 2016, 24(5):1062-1067.
本文引用 [1]
[22]
赵婕, 张子晗, 侯秋彦, 等. 东京野茉莉种子休眠特性的研究[J]. 中南林业科技大学学报, 2019, 39(1):45-51.
本文引用 [1]
[23]
周峰, 王海鸥, 华春, 等. 植物种子萌发的生物力学[J]. 种子, 2019, 38(3):50-52,56.
本文引用 [1]
[24]
张心妍. 流苏种子解剖及休眠解除方法研究[D]. 秦皇岛: 河北科技师范学院, 2021.
本文引用 [1]
[25]
李欣勇, 黄迎, 张静文. 风车草种子休眠及萌发特性研究[J]. 种子, 2021, 40(5):57-62.
本文引用 [1]
[26]
尹德洁, 布凤琴, 徐艳芳, 等. 单叶蔓荆种子休眠特性与解除方法[J]. 林业科学, 2020, 56(12):157-165.
本文引用 [1]
[27]
王伟青, 程红焱. 拟南芥突变体种子休眠与萌发的研究进展[J]. 植物学通报, 2006, 23(6):625-633.
本文引用 [1]
[28]
Peng J R, NICHOLAS P HARBERD. The role of GA-mediated signalling in the control of seed germination[J]. Current opinion in plant biology: 2002, 5(5):376-381.
https://www.ncbi.nlm.nih.gov/pubmed/12183174
本文引用 [1] 摘要
Seed germination is promoted by gibberellin (GA) in many plant species. Several GA signalling factors are known to induce the expression of genes encoding enzymes that mobilise food reserves, including starches, proteins and lipids, stored in the endosperm during seed germination. However, these factors do not control seed germination. Two recent reports have indicated that RGL1 and RGL2, both homologous to the GA-response height-regulating factors GAI/RGA/RHT/d8/SLR1/SLN1, are repressors of seed germination in Arabidopsis. These reports provide new clues as to how GA controls seed germination. The induction of RGL2 expression by imbibition and its repression by GA are of particular interest because they imply that RGL2 acts as an integrator of environmental and endogenous cues for germination.
[29]
GROOT S P C, KIELISZEWSKA-ROKICKA B, VERMEER E, et al. Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radical protrusion[J]. Planta, 1988, 174:500-504.
https://doi.org/10.1007/BF00634479
http://link.springer.com/10.1007/BF00634479
本文引用 [1]
[30]
TOOROP P E, BEWLEY J D, HILHORST H W M. Endo-β-mannanase isoforms are present in the endosperm and embryo of tomato seeds, but are not essentially linked to the completion of germination[J]. Planta, 1996, 200:153-158.
本文引用 [1]
[31]
MO B, BEWLEY J D. β-mannosidase activity during and fzollowing germination of tomato seed[J]. Planta, 2002, 215:141-152.
https://doi.org/10.1007/s00425-001-0725-x
http://link.springer.com/10.1007/s00425-001-0725-x
本文引用 [1]
[32]
王因花. 南京椴种子休眠机理研究[D]. 南京: 南京林业大学, 2007.
本文引用 [1]
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