PDF(2222 KB)
Chlorophyll Fluorescence Characteristics of Lilium spp. Cultivars Under Natural High Temperature
CHEN Minmin, NIE Gongping, LI Xin, ZHOU Lin, YANG Liuyan, ZHANG Yongchun
PDF(2222 KB)
PDF(2222 KB)
Chlorophyll Fluorescence Characteristics of Lilium spp. Cultivars Under Natural High Temperature
A study on the response of chlorophyll fluorescence parameters of different Lilium spp. cultivars to natural high temperature was carried out to analyze the important indicators reflecting the characteristics of chlorophyll fluorescence, establish a suitable method for evaluating the high temperature response of Lilium spp. cultivars, and select sensitive and insensitive cultivars to natural high temperature. 66 Lilium spp. cultivars were selected as experimental materials, and the Fv/Fm values of functional leaves were measured under natural high temperature in Shanghai. The fuzzy membership function analysis method and the sample optimal segmentation clustering method were adopted to evaluate the heat tolerance. Under natural high temperature conditions, the chlorophyll fluorescence characteristic parameters including Fo, Fm, Fv/Fo, Fv/Fm, PIABS, DIo/CSo and φEo in leaves were determined by rapid fluorescence detector. Then, we compared the sensitivity of different Lilium spp. cultivars. Results showed that the Fv/Fm values of the 66 cultivars were all influenced by natural high temperature. The sample optimal segmentation clustering method was used to classify the 66 cultivars into five grades. 25 cultivars, including ‘Souvenir’, ‘Merlet’, ‘Conca D'Or’, ‘Sunny Martinique’, ‘Methone’ and so on, belonged to grade I, which were not sensitive to high temperature and had strong adaptability. 19 cultivars, including ‘Zambesi’, ‘Triumphator’, ‘Nashville’, ‘Brindisi’, ‘Tresor’, etc., belonged to grade V, which were more sensitive to high temperature and had poor adaptability. This study screened and identified sensitive and insensitive Lilium spp. cultivars to high temperature, which could provide reference for further research on heat-resistant mechanism of Lilium spp. and breeding heat-resistant cultivars.
Lilium spp. / chlorophyll fluorescence parameters / natural high temperature response / membership function method {{custom_keyword}} /
| [1] |
刘雪凝, 杨利平. 热激锻炼对亚洲百合耐热性的诱导[J]. 东北林业大学学报, 2011, 39(5):64-66.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [2] |
李蕊, 杨利平, 刘雪凝. 百合杂交种及亲本耐热性比较[J]. 中国农业科学, 2011, 44(6):1201-1209.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [3] |
刘艳妮, 佘奎军, 王飞. 百合耐热突变体的筛选及生理特性的研究[J]. 西北农业学报, 2012, 21(12):131-137.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [4] |
张铭顺, 吕福堂, 褚鹏飞, 等. 外源Ca2+提高百合耐热性的生理机制初探[J]. 河南农业科学, 2019, 48(2):120-125.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [5] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [6] |
Based on studies of monocot crops and eudicot model plants, the DREB2 class of AP2-type transcription factor has been shown to play crucial roles in various abiotic stresses, especially in the upstream of the heat stress response; however, research on DREB2s has not been reported in non-gramineous monocot plants. Here, we identified a novel DREB2 (LlDREB2B) from lily (Lilium longiflorum), which was homologous to AtDREB2A of Arabidopsis, OsDREB2B of rice, and ZmDREB2A of maize. LlDREB2B was induced by heat, cold, salt, and mannitol stress, and its protein had transcriptional activity, was located in the nucleus, was able to bind to the dehydration-responsive element (DRE), and participated in the heat-responsive pathway of HsfA3. Overexpression of LlDREB2B in Arabidopsis activated expression of downstream genes and improved thermotolerance. LlDREB2B was not regulated by alternative splicing; functional transcripts accumulated under either normal or heat-stress conditions. A potential PEST sequence was predicted in LlDREB2B, but the stability of the LlDREB2B protein was not positively affected when the predicated PEST sequence was deleted. Further analysis revealed that the predicated PEST sequence lacked a SBC or SBC-like motif allowing interaction with BPMs and required for negative regulation. Nevertheless, LlDREB2B was still regulated at the post-translational level by interaction with AtDRIP1 and AtDRIP2 of Arabidopsis. In addition, LlDREB2B also interacted with AtRCD1 and LlRCD1 via a potential RIM motif located at amino acids 215-245. Taken together, our results show that LlDREB2B participated in the establishment of thermotolerance, and its regulation was different from that of the orthologs of gramineous and eudicot plants.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [7] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [8] |
张佳平, 李丹青, 聂晶晶, 等. 高温胁迫下芍药的生理生化响应和耐热性评价[J]. 核农学报, 2016, 30(9):1848-1856.
南方夏季的高温胁迫,使芍药茎叶因热害提早枯萎,并导致地下块根更新发育不良,是“芍药南移”所面临的主要困难。为探讨南方夏季高温胁迫下芍药的生理生化响应,比较耐热性评价方法,以浙江本土药用植物杭白芍和9个菏泽的观赏品种为材料,对夏季高温下的芍药茎叶进行热害指数观测,测定叶片的相对电导率、相对含水量、叶绿素含量、可溶性糖含量、丙二醛含量、可溶性蛋白含量、抗坏血酸过氧化物酶活性和超氧化物歧化酶活性;并利用隶属函数法、隶属函数法结合主成分分析2种方法对10个品种的耐热性展开评价。结果表明,杭白芍、杨妃出浴和春晓具备较好的耐热性,适合江南地区选用;隶属函数法是芍药耐热评价的简便、直观、有效的评价方法。本研究结果为“芍药南移”及抗性育种研究提供了重要参考。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [9] |
王海珍, 韩路, 徐雅丽, 等. 胡杨异形叶叶绿素荧光特性对高温的响应[J]. 生态学报, 2011, 31(9):2444-2453.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [10] |
郭连旺, 许大全. 自然条件下珊瑚树叶片光合作用的光抑制[J]. 植物生理学报, 1994, 20(1):46-54.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [11] |
王春萍, 张世才, 雷开荣, 等. 辣椒苗期耐低氮指标与评价方法研究[J]. 园艺学报, 2017, 44(12):2318-2326.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [12] |
Global warming is predicted to have a general negative effect on plant growth due to the damaging effect of high temperatures on plant development. The increasing threat of climatological extremes including very high temperatures might lead to catastrophic loss of crop productivity and result in wide spread famine. In this review, we assess the impact of global climate change on the agricultural crop production. There is a differential effect of climate change both in terms of geographic location and the crops that will likely show the most extreme reductions in yield as a result of expected extreme fluctuations in temperature and global warming in general. High temperature stress has a wide range of effects on plants in terms of physiology, biochemistry and gene regulation pathways. However, strategies exist to crop improvement for heat stress tolerance. In this review, we present recent advances of research on all these levels of investigation and focus on potential leads that may help to understand more fully the mechanisms that make plants tolerant or susceptible to heat stress. Finally, we review possible procedures and methods which could lead to the generation of new varieties with sustainable yield production, in a world likely to be challenged both by increasing population, higher average temperatures and larger temperature fluctuations.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [13] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [14] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [15] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [16] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [17] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [18] |
张永平, 陈幼源, 杨少军, 等. 外源水杨酸对镉胁迫甜瓜幼苗生长与光合气体交换和叶绿素荧光特性的影响[J]. 西北植物学报, 2014, 34(4):778-785.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [19] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [20] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [21] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [22] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [23] |
查倩, 奚晓军, 蒋爱丽, 等. 高温胁迫对葡萄幼树叶绿素荧光特性和抗氧化酶活性的影响[J]. 植物生理学报, 2016, 52(4): 525-532.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [24] |
吴久赟, 廉苇佳, 刘志刚, 等. 不同葡萄品种叶绿素荧光参数的高温响应及其耐热性评价[J]. 西北农林科技大学学报(自然科学版), 2019, 47(6):80-88.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [25] |
徐新翔, 侯昕, 王芬, 等. 低钾胁迫对苹果砧木M9T337幼苗光合荧光特性及13C吸收分配的影响[J]. 园艺学报, 2020, 47(3):529-540.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [26] |
张鹏, 周泉, 黄国勤. 冬季不同种植模式对稻田土壤团聚体及其有机碳的影响[J]. 核农学报, 2019, 33(12):2430-2438.
为探究冬季不同种植模式对稻田土壤结构和质量的影响,设置5种冬季种植模式:冬季休闲(冬闲)、冬种紫云英、冬种油菜、冬种大蒜和冬季轮作(马铃薯、紫云英、油菜),通过测定稻田不同土层土壤团聚体组成和团聚体有机碳含量,分析稻田土壤团聚体的水稳性和团聚体有机碳分布。结果表明,在0~30 cm土层深度:>2 mm粒级的土壤团聚体含量最高,其次是<0.053 mm粒级的土壤团聚体,1~2、0.5~1、0.25~0.05、0.053~0.25 mm粒级的土壤团聚体含量相近;各冬季种植处理模式均提高了稻田水稳性团聚体的平均质量直径(MWD)和平均几何直径(GMD),其中冬种油菜、冬季轮作以及冬种紫云英处理的MWD较冬闲处理分别显著提高21.50%、21.16%、16.32%(P<0.05),冬种油菜和冬季轮作处理的GMD较冬闲处理分别显著提高83.31%、62.50%(P<0.05);冬季不同种植模式显著降低了土壤水稳性团聚体的分形维数(P<0.05),有利于维持土壤结构的稳定性;相对于冬闲处理,其余不同冬季种植模式处理的各粒径下土壤团聚体有机碳含量有增加的趋势。综上可知,冬季不同种植模式均有利于稻田土壤团聚体稳定性的提高和各粒级下团聚体有机碳的积累,其中冬季轮作模式的效果最佳。本研究结果为南方冬闲田优化种植模式提供了理论依据。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [27] |
俞华先, 田春艳, 经艳芬, 等. 水分胁迫对4份含大茎野生种57NG208血缘F2代甘蔗材料叶绿素荧光动力参数的影响[J]. 中国农学通报, 2019, 35(28):17-24.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [28] |
杨卫丽, 黄福灯, 曹珍珍, 等. 高温胁迫对水稻光合PSII系统伤害及其与叶绿体D1蛋白间关系[J]. 作物学报, 2013, 39(6):1060-1068.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [29] |
龙雅宜, 张金政, 张兰年. 百合-球根花卉之王[M]. 北京: 金盾出版社, 1999:116.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| {{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
PDF(2222 KB)
/
| 〈 |
|
〉 |
