Pedigree analysis can simply and effectively elucidate the integrated genetic basis of breeding varieties, and grasping the genetic basis of breeding varieties is helpful to reveal the characteristics of variety evolution, summarize the experience of parental selection and guide variety breeding. This research analyzed the breeding methods and the variety pedigree of 21 barley varieties bred by Baoshan Institution of Agricultural Sciences since 2000. The results show that the hybrid breeding is the main barley breeding method of Baoshan Institution of Agricultural Sciences, 76.2% of the varieties were bred through cross-breeding, 23.8% of the varieties were bred through systematic selection. The 21 barley varieties were derived from 20 cell nucleus ancestor parents and 10 cytoplasm ancestor parents. The parental cell nucleus from Mexico gave the largest contribution value, which was 62.35%, indicating that the ancestral parents of Baodamai series were mainly from Mexico. The parents ‘YS500’, ‘V008’, ‘8640’ and ‘Peaosanhos-174’ from Mexico had the largest cumulative cell nucleus contribution value and most derived varieties, which were the core ancestral parents.