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224 Maize Inbred Lines from Shanxi: Genetic Structure and Genetic Relationships Based on SSR Markers by Fluorescence Detection
LI Rui, SHANG Xiao, SHANG Chunshu, CHANG Lifang, YAN Lei, BAI Jianrong
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224 Maize Inbred Lines from Shanxi: Genetic Structure and Genetic Relationships Based on SSR Markers by Fluorescence Detection
In order to use the heterosis, it is necessary to analyze the genetic structure and genetic relationships of maize inbred lines. Based on the analysis of 40 SSR loci, the genetic structure and genetic relationships of 224 maize inbred lines from Shanxi were analyzed. A total of 171 alleles were detected with 4.28 alleles per locus. The average of the PIC, MI and gene diversity was 0.44, 2.02 and 0.50 respectively. There were 9 unique alleles among 9 SSR loci. These lines could be divided into three groups by model-based cluster method, which were Reid, Lancaster and SPT. According to the result of genetic structure component (Q-value), there were some cross-group inbred lines in each group. The 224 lines could be divided into 4 groups by distance-based cluster method, which were Reid, Lancaster, SPT and Others. About 90% inbreed lines were included in Reid group and Lancaster group. This study also discussed the choice of tester for heterotic groups, the types of SSR markers and cluster methods of heterotic grouping in maize commercial breeding. The study could provide a basis for the improvement of inbreed lines and the selection of new hybrid combinations in maize commercial breeding.
maize / inbreed lines / SSR / genetic structure / genetic relationship {{custom_keyword}} /
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