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Preventing Transgene Drift of Maize: The Test Strip Rapid Detection Method
CUI Guimei, LI Xiaobo, MA Yue, HAO Yaoshan, ZHANG Huanhuan, SUN Yi
PDF(1488 KB)
PDF(1488 KB)
Preventing Transgene Drift of Maize: The Test Strip Rapid Detection Method
The purpose of this study is to detect transgenic plants from conventional maize breeding materials at seedling stage by using test strips, and to prevent unexpected transgene drift. Transgenic and non-transgenic maize lines were used as materials in this study. Based on the experiences of screening transgenic maize materials in the past, test strip rapid detection method was obtained. The authors introduced the supplies required for examination and sampling methods, and discussed the sample quantity, operation steps and precautions in detail. The authors also demonstrated and analyzed the processes and results of two typical detection cases. The operation steps of this method are simple and easy to learn, and the method is suitable for professional and non-professional breeders or inspectors to use in self-examination or inspection of field materials. The test strip rapid detection method can effectively screen and prevent the transgene drift caused by unexpected mixing of pollen and seeds.
maize / transgene drift / pollen / the test strip rapid detection {{custom_keyword}} /
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感谢山西南繁育种基地田齐建研究员、利进才老师等人提供检测玉米材料。
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