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Influence of Municipal Solid Waste Stacking on Soil Bacterial Community Structure and Function
Cheng Zhichao, Wu Wenyan, Song Yonghui, Sui Xin, Li Mengsha, Yang Libin
PDF(2194 KB)
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Influence of Municipal Solid Waste Stacking on Soil Bacterial Community Structure and Function
To explore the effect of municipal solid waste stacking on the structure, diversity and function of soil bacteria, by using the Illumina high-throughput sequencing technology, we investigated the soil bacterial community structure and function from the municipal solid waste stacking soil and the no waste stacking soil 50 m away from waste stacking soil in Shuangcheng District, Harbin. The results showed that the control group and the treatment group obtained 85435 and 76432 effective sequences, respectively; compared with the control soil, the Chao1 index of bacteria in treatment soil decreased by 14.47% and the Shannon index decreased by 15.01%; the dominant bacterial phyla of two treatment soils were Proteobacteria, Bacteroidetes, Chloroflexi, Acidobacteria, Actinobacteria, Gemmatimonadetes and Cyanobacteria, but the relative abundances were significantly different, in which Chloroflexi increased most by 7.0% and Bacteroidetes decreased most by 12.6%. The relative abundances of Bacillus and Xanthomonas were the highest. Compared with the control treatment, the relative abundance of Bacillus increased by 4.3%. The results of PICRUSt prediction showed that there were 41 KEGG secondary functional groups in the 6 metabolic pathways, of which amino acid metabolism and carbohydrate metabolism were the dominant functions of the two soil samples in the secondary functional groups. The solid waste stacking had a significant impact on the abundance of functional genes. Solid waste stacking has significant negative effect on the abundance and diversity of soil bacteria, as well as a significant effect on the relative abundance of soil bacterial communities and their function, and the study result could provide theoretical data for elucidating the soil quality after solid waste stacking.
household garbage / soil bacteria / high-throughput sequencing / PICRUSt function prediction {{custom_keyword}} /
表1 土壤样品的微生物多样性指数 |
| Sample | Sobs | Shannon | Simpson | Ace | Chao1 | Coverage/% |
|---|---|---|---|---|---|---|
| 对照 | 1921.0±136.7a | 6.2±0.1a | 0.007±0.002a | 2242.7±127.3a | 2249.3±118.3a | 98±0.00a |
| 处理 | 1646.3±61.78b | 5.8±0.12b | 0.012±0.003a | 1906.1±66.1b | 1923.7±92.2b | 98±0.00a |
表2 土壤细菌群落功能丰度表 |
| 一级功能 | 二级功能 | C | 百分比/% | T | 百分比/% | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Metabolism | Amino Acid Metabolism | 2854091 | 10.47 | 2331140 | 10.18 | |||||||||
| Metabolism | Carbohydrate Metabolism | 2670399 | 9.80 | 2206482 | 9.64 | |||||||||
| Environmental Information Processing | Membrane Transport | 2604045 | 9.56 | 2383110 | 10.41 | |||||||||
| Genetic Information Processing | Replication and Repair | 2021780 | 7.42 | 1708886 | 7.46 | |||||||||
| Metabolism | Energy Metabolism | 1804503 | 6.62 | 1491229 | 6.51 | |||||||||
| Unclassified | Poorly Characterized | 1398465 | 5.13 | 1172485 | 5.12 | |||||||||
| Genetic Information Processing | Translation | 1301802 | 4.78 | 1122984 | 4.90 | |||||||||
| Metabolism | Metabolism of Cofactors and Vitamins | 1245312 | 4.57 | 1025830 | 4.48 | |||||||||
| Unclassified | Cellular Processes and Signaling | 1034875 | 3.80 | 871649 | 3.81 | |||||||||
| Metabolism | Lipid Metabolism | 984298 | 3.61 | 804376 | 3.51 | |||||||||
| Metabolism | Nucleotide Metabolism | 925572 | 3.40 | 774079 | 3.38 | |||||||||
| Cellular Processes | Cell Motility | 911201 | 3.34 | 863550 | 3.77 | |||||||||
| Metabolism | Xenobiotics Biodegradation and Metabolism | 778081 | 2.86 | 593932 | 2.59 | |||||||||
| Unclassified | Genetic Information Processing | 735318 | 2.70 | 615411 | 2.69 | |||||||||
| Genetic Information Processing | Folding, Sorting and Degradation | 708558 | 2.60 | 616715 | 2.69 | |||||||||
| Unclassified | Metabolism | 700475 | 2.57 | 570276 | 2.49 | |||||||||
| Metabolism | Glycan Biosynthesis and Metabolism | 627282 | 2.30 | 536108 | 2.34 | |||||||||
| Environmental Information Processing | Signal Transduction | 617938 | 2.27 | 531786 | 2.32 | |||||||||
| Genetic Information Processing | Transcription | 605085 | 2.22 | 496863 | 2.17 | |||||||||
| 一级功能 | 二级功能 | C | 百分比/% | T | 百分比/% | |||||||||
| Metabolism | Metabolism of Terpenoids and Polyketides | 580423 | 2.13 | 467502 | 2.04 | |||||||||
| Metabolism | Enzyme Families | 552628 | 2.03 | 462769 | 2.02 | |||||||||
| Metabolism | Metabolism of Other Amino Acids | 499610 | 1.83 | 390798 | 1.71 | |||||||||
| Metabolism | Biosynthesis of Other Secondary Metabolites | 279783 | 1.03 | 227262 | 0.99 | |||||||||
| Cellular Processes | Cell Growth and Death | 162926 | 0.60 | 131388 | 0.57 | |||||||||
| Human Diseases | Infectious Diseases | 116452 | 0.43 | 90288 | 0.39 | |||||||||
| Organismal Systems | Endocrine System | 109991 | 0.40 | 90806 | 0.40 | |||||||||
| Cellular Processes | Transport and Catabolism | 89898 | 0.33 | 70245 | 0.31 | |||||||||
| Human Diseases | Neurodegenerative Diseases | 86995 | 0.32 | 68964 | 0.30 | |||||||||
| Environmental Information Processing | Signaling Molecules and Interaction | 46054 | 0.17 | 34673 | 0.15 | |||||||||
| Organismal Systems | Environmental Adaptation | 40935 | 0.15 | 35208 | 0.15 | |||||||||
| Human Diseases | Cancers | 40283 | 0.15 | 26719 | 0.12 | |||||||||
| Human Diseases | Metabolic Diseases | 23895 | 0.09 | 18772 | 0.08 | |||||||||
| Organismal Systems | Nervous System | 22358 | 0.08 | 17221 | 0.08 | |||||||||
| Organismal Systems | Immune System | 15163 | 0.06 | 12411 | 0.05 | |||||||||
| Organismal Systems | Digestive System | 13605 | 0.05 | 8702 | 0.04 | |||||||||
| Organismal Systems | Circulatory System | 11550 | 0.04 | 10397 | 0.05 | |||||||||
| Human Diseases | Immune System Diseases | 10862 | 0.04 | 7727 | 0.03 | |||||||||
| Organismal Systems | Excretory System | 10817 | 0.04 | 8228 | 0.04 | |||||||||
| Human Diseases | Cardiovascular Diseases | 4608 | 0.02 | 1683 | 0.01 | |||||||||
| Cellular Processes | Cell Communication | 54 | 0.00 | 36 | 0.00 | |||||||||
| Organismal Systems | Sensory System | 18 | 0.00 | 12 | 0.00 | |||||||||
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PDF(2194 KB)
表1 土壤样品的微生物多样性指数
图1 不同处理土壤样品在门分类水平上细菌组成图2 不同处理土壤样品在属分类水平上细菌组成图3 垃圾堆放对土壤细菌群落门(A)和属(B)水平的差异分析图4 不同处理土壤细菌在属分类水平上的Venn图表2 土壤细菌群落功能丰度表/
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