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Biocontrol Bacteria Against Northern Corn Leaf Blight: Screening, Identification and Application
Zhang Qifeng, Li Yonggang, Liu Bo
PDF(2295 KB)
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Biocontrol Bacteria Against Northern Corn Leaf Blight: Screening, Identification and Application
In order to explore the biological way to effectively control the damage of northern corn leaf blight in corn production, Setosphaeria turcica was used as the targeted strain. The biocontrol bacteria were obtained using confrontation method and pot inoculation test. The control effect of pot inoculation test was determined. The results showed that one strain with the best antagonistic effect was obtained from 128 strains of endophytic bacteria. The ratio of the longest radius to the shortest radius was 2.9. The biocontrol bacteria YD7 was screened from corn endophytic bacteria and it had relatively strong antagonistic effect against S. turcica. According to the morphological, physiology-biochemical characteristics and 16S rDNA sequence analysis, YD7 was identified as Bacillus amyloliquefaciens. By two potted experiments, the control effects of YD7 were 78.2% and 77.8%, respectively. Thus, YD7 has well developing prospect on controlling northern corn leaf blight.
northern corn leaf blight / biocontrol bacteria / antagonistic effect / identification {{custom_keyword}} /
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