PDF(1407 KB)
Effects of Acetoin Addition on 2,3-butanediol Production by Saccharomyces cerevisiae W5/W141
Yang Zhiyu, Tong Tianqi, Liu Lei, Ping Wenxiang, Ge Jingping
PDF(1407 KB)
PDF(1407 KB)
Effects of Acetoin Addition on 2,3-butanediol Production by Saccharomyces cerevisiae W5/W141
To obtain a 2,3-butanediol (2,3-BD) producing strain with good performance, different concentrations of acetoin were added into two strains of Saccharomyces cerevisiae (W5/W141) producing 2,3-BD, and the changes of 2,3-BD, ethanol and glycerol production with fermentation time were determined. The optimum acetoin concentration of S. cerevisiae W5 was 12 g/L, and the concentration of 2,3-BD reached the maximum at 72 h, it was 2.54±0.03 g/L, and the conversion of glycerol and ethanol decreased by 17.6% and 23.6% respectively compared with that without addition. The optimum acetoin concentration of S. cerevisiae W 141 was 10 g/L, and the concentration of 2,3-BD reached the maximum at 72 h, it was 1.71±0.02 g/L, and the conversion of glycerol and ethanol decreased by 57.1% and 16.7% respectively. By comparison, S. cerevisiae W5 was selected as the optimum strain to provide new resources for the production of 2,3-BD by microbial fermentation.
2,3-butanediol / Saccharomyces cerevisiae / acetoin / ethanol / glycerol {{custom_keyword}} /
| [1] |
2,3-butanediol is a promising bulk chemical due to its extensive industry applications. The state-of-the-art nature of microbial 2,3-butanediol production is reviewed in this paper. Various strategies for efficient and economical microbial 2,3-butanediol production, including strain improvement, substrate alternation, and process development, are reviewed and compared with regard to their pros and cons. This review also summarizes value added derivatives of biologically produced 2,3-butanediol and different strategies for downstream processing. The future prospects of microbial 2,3-butanediol production are discussed in light of the current progress, challenges, and trends in this field. Guidelines for future studies are also proposed.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [2] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [3] |
王爽. 2,3-丁二醇脱水制备甲乙酮的催化剂及工艺研究[D]. 辽宁:大连理工大学, 2014.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [4] |
戴建英, 孙亚琴, 孙丽慧, 等. 生物基化学品2,3-丁二醇的研究进展[J]. 化学工程学报, 2010,10(1):200-208.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [5] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [6] |
刘佳娴, 朱家文, 吴艳阳, 等. 2,3-丁二醇分离纯化中反应精馏的实验和模拟[J]. 化学反应工程与工艺, 2012(2):104-110.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [7] |
葛岚, 邵晓丛, 吴晓敏, 等. 工业化制备2,3-丁二醇的新途径[J]. 科技创新导报, 2009(33):6-106.
本文基于笔者多年从事虚拟现实相关技术的研究,以大型展馆网上发布为研究对象,探讨了基于虚拟场景建模的大型展馆建模技术,论文首先分析了LOD处理技术的应用,探讨了系统的构架,研究了基于JAVA的网上发布系统的设计,全文是笔者长期工作实践基础上的理论升华,相信对从事相关工作的同行有着重要的参考价值和借鉴意义.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [8] |
李亿, 李检秀, 刘海余, 等. 多粘类芽孢杆菌同步糖化发酵玉米粉生产(R,R) -2,3-丁二醇[J]. 广西科学, 2016,23(1):43-46.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [9] |
The Klebsiella oxytoca was engineered to produce 2,3-butanediol (2,3-BDO) simultaneously utilizing glucose and galactose obtained from a Golenkinia sp. hydrolysate. For efficient uptake of galactose at a high concentration of glucose, Escherichia coli galactose permease (GalP) was introduced, and the expression of galP under a weak-strength promoter resulted in simultaneous consumption of galactose and glucose. Next, to improve the sugar consumption, a gene encoding methylglyoxal synthase (MgsA) known as an inhibitor of multisugar metabolism was deleted, and the mgsA-null mutant showed much faster consumption of both sugars than the wild-type strain did. Finally, we demonstrated that the engineered K. oxytoca could utilize sugar extracts from a Golenkinia sp. hydrolysate and successfully produces 2,3-BDO.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [10] |
Mutants with overexpression of alpha-acetolactate synthase (ALS), alpha-acetolactate decarboxylase, and acetoin reductase (AR), either individually or in combination, were constructed to improve 2,3-butanediol (2,3-BD) production in Klebsiella pneumoniae. The recombinant strains were characterized in terms of the enzyme activity, 2,3-BD yield, and expression levels. The recombinant K. pneumoniae strain (KG-rs) that overexpressed both ALS and AR showed an improved 2,3-BD yield. When cultured in the media with five different carbon sources (glucose, galactose, fructose, sucrose, and lactose), the mutant exhibited higher 2,3-BD productivity and production than the parental strain in all the tested carbon sources except for lactose. The 2,3-BD production of KG-rs in a batch fermentation with glucose as the carbon source was 12% higher than that of the parental strain. (c) 2014 International Union of Biochemistry and Molecular Biology, Inc.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [11] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [12] |
张刚, 杨光, 李春, 等. 生物法生产2,3-丁二醇研究进展[J]. 中国生物工程杂志, 2008,28(6):133-140.
2,3-丁二醇是一种重要的化工原料，可广泛应用于多个领域。二战期间由于合成橡胶需要大量1,3-丁二烯，2,3-丁二醇生产空前发展。近年来，由于聚对苯二甲酸丁烯树脂、γ-丁内酯，Spandex弹性纤维及其前体的需求增长，2,3-丁二醇的需求和产量也稳步增长。多年来，生物法生产2,3-丁二醇虽然得到了广泛的研究，但一直没有实现工业化。本文从产生2,3-丁二醇的菌种及2,3-丁二醇的生理意义、代谢途径、旋光异构体的形成机理、影响发酵的因素与产物的提纯等方面对生物法生产2,3-丁二醇进行了综述并提出了生物法生产2,3-丁二醇要解决的几个问题。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [13] |
王青艳, 谢能中, 黎贞崇, 等. 微生物法合成(R,R)-2,3-丁二醇的研究进展与展望[J]. 基因组学与应用生物学, 2014 , 33(6):1367-1373.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [14] |
纪晓俊, 朱建国, 高振, 等. 微生物发酵法生产2,3-丁二醇的研究进展[J]. 现代化工, 2006,26(8):23-27.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [15] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [16] |
Fermentative 2,3-butanediol (2,3-BD) production has been receiving increasing interest for its potential as a platform chemical intended for the production of synthetic rubbers, plastics, and solvents. In this study, Klebsiella oxytoca GSC 12206, a 2,3-BD native hyper-producing and nonpathogenic bacterium, was isolated from a cattle farm. Since this isolate produced a significant amount of lactic acid along with 2,3-BD, its mutant deficient in lactic acid formation was constructed by disrupting the ldhA gene which encodes lactate dehydrogenase. The ldhA gene was deleted precisely by using the pKGS plasmid. When compared to the wild-type strain, the mutant deleted with the ldhA gene in glucose fermentation resulted in an increase of 54%, 13%, 60%, and 78% of 2,3-BD titer, productivity, yield, and selectivity, respectively. A fed-batch fermentation by this mutant with intermittent glucose feeding produced 115 g/L of 2,3-BD with an yield and productivity of 0.41 g 2,3-BD per g glucose and 2.27 g/L h, respectively, indicating the usefulness for the industrial production of 2,3-BD. (c) 2013, The Society for Biotechnology, Japan.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [17] |
In order to produce 2,3-butanediol (2,3-BD) with a high titer, it is necessary to engineer Saccharomyces cerevisiae by deleting the competing pathway and overexpressing the 2,3-BD biosynthetic pathway. A pyruvate decarboxylase (Pdc)-deficient mutant was constructed and evolved for rapid glucose consumption without ethanol production. Genome re-sequencing of the evolved strain (SOS4) revealed a point mutation (A81P) in MTH1 coding for a transcriptional regulator involved in glucose sensing, unlike the previously reported Pdc-deficient mutant which had internal deletion in MTH1. When alsS and alsD genes from Bacillus subtilis, and endogenous BDH1 gene were overexpressed in SOS4, the resulting strain (BD4) not only produced 2,3-BD efficiently, but also consumed glucose faster than the parental strain. In fed-batch fermentation with optimum aeration, 2,3-BD concentration increased up to 96.2 g/L. These results suggest that S. cerevisiae might be a promising host for producing 2,3-BD for industrial applications. (c) 2013 Elsevier Ltd.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [18] |
宋洋波, 马捷, 李丽, 等. 后基因组时代的酿酒酵母研究策略[J]. 中国农业科学, 2012,45(23):4873-4882.
近年，随着基因工程技术的日益发展，酿酒酵母在分子生物学研究方面的应用越来越广泛。本文介绍了后基因组学用于酿酒酵母的方法，并概述了利用该手段对试验菌株与商业酿酒酵母进行生理功能分析、研究和改造的最新进展，展示了后基因组时代对酿酒酵母的影响及综合基因组信息、生物信息学和分子生物学技术的研究策略。最后，展望了现代生物技术手段在葡萄酒工业中的应用前景。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [19] |
闫道江, 王彩霞, 周杰民, 等. 酿酒酵母产苹果酸的还原TCA路径构建及发酵调控[J]. Chinese Journal of Biotechnology, 2013,29(10):1484-1493.
Malic acid is widely used in food, and chemical industries. Through overexpressing pyruvate carboxylase and malate dehydrogenase in pdc1-deficient Saccharomyces cerevisiae, malic acid was successfully produced through the reductive TCA pathway. No malic acid was detected in wild type Saccharomyces cerevisiae, however, 45 mmol/L malic acid was produced in engineered strain, and the concentration of byproduct ethanol also reduced by 18%. The production of malic acid enhanced 6% by increasing the concentration of Ca2+. In addition, the final concentration reached 52.5 mmol /L malic acid by addition of biotin. The increasing is almost 16% higher than that of the original strain.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [20] |
刘德安, 王长丽, 丁昊, 等. 代谢工程改造酿酒酵母生产2,3-丁二醇的研究进展[J]. 中国酿造, 2018,37(09):12-17.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [21] |
黄守锋, 裴芳艺, 王长丽, 等. 利用酿酒酵母工程菌株生产2,3-丁二醇的研究进展[J].食品安全质量检测学报, 2015(10):3928-3934.
2,3-丁二醇在食品、化妆品、医药和运输燃料等行业具有广泛的应用, 因而提高2,3-丁二醇的产量一直备受研究者们关注。目前, 研究的热点主要集中于利用微生物发酵可再生资源生产2,3-丁二醇以取代传统的化学合成法, 并取得了较大的进展。常见的2,3-丁二醇产生菌有克雷伯氏菌属和芽孢杆菌属, 它们能有效利用可再生资源高效生产2,3-丁二醇。然而这些细菌被认为是潜在的病原菌, 难以应用于大规模生产。因此, 研究者们又将目光转向了酿酒酵母。就安全性和工业化生产要求而言, 酿酒酵母是生产2,3-丁二醇的理想菌种。本文对国内外的相关研究进行了总结, 介绍了酿酒酵母产2,3-丁二醇的优势和不足, 2,3-丁二醇合成代谢途径及其基因工程菌株构建的方向, 以及通过代谢工程将酿酒酵母改造成能高效、高质、高量产生2,3-丁二醇的理想菌株的研究关键。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [22] |
佟天奇, 裴芳艺, 王长丽, 等. 酿酒酵母(Saccharomyces cerevisiae)WBG3菌株发酵特性研究[J]. 中国农学通报, 2017,34(32).
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| {{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
PDF(1407 KB)
/
| 〈 |
|
〉 |
