Diversity Analysis of Prokaryotic Microorganism of Farmland Soil: Bailang Village, Linzhou County, Lhasa

Jin Guimei, De Ji, Yang Sang, Baima quwang, Gesang yuzhen, Guo Xiaofang

PDF(1266 KB)
PDF(1266 KB)
Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (6) : 74-81. DOI: 10.11924/j.issn.1000-6850.casb18110035

Diversity Analysis of Prokaryotic Microorganism of Farmland Soil: Bailang Village, Linzhou County, Lhasa

Author information +
History +

Abstract

The paper aims to explore the differences of microflora composition structure among different farmland types by studying the microorganism diversity of farmland soil in Linzhou, Lhasa. We isolated bacteria and actinomycetes from farmland soil and analyzed the 16S rDNA sequence, combined with the classic classification, we classified and identified the obtained strains, and finally determined the microflora composition structure of farmland soil. The results showed that soil bacteria were classified into 6 genera and 15 species, and actinomycetes were divided into 13 species; the occurrence frequency and relative abundance indicated that the dominant bacteria in soil were Bacillus cereus and Bacillus simple, however the dominant actinomyces were Streptomyces pseudogriseolus, Streptomyces ciscaucasicus and Streptomyces flavogriseus. Crop types have no significant influence on community composition of bacteria and actinomyces from farmland soil in Bailang village, Linzhou County.

Key words

Tibet plateau / farmland soil / bacteria / actinomycetes / microflora / crop types

Cite this article

Download Citations
Jin Guimei , De Ji , Yang Sang , Baima quwang , Gesang yuzhen , Guo Xiaofang. Diversity Analysis of Prokaryotic Microorganism of Farmland Soil: Bailang Village, Linzhou County, Lhasa. Chinese Agricultural Science Bulletin. 2020, 36(6): 74-81 https://doi.org/10.11924/j.issn.1000-6850.casb18110035

References

[1]
毕明丽, 宇万太 . 农田生态系统微生物多样性研究方法及应用[J]. 土壤学报, 2009,40(6):1460-1466.
[2]
林先贵, 胡君利 . 土壤微生物多样性的科学内涵及其生态服务功能[J]. 土壤学报, 2018,45(5):892-890.
[3]
莫申国, 张百平, 程维明 , 等. 青藏高原的主要效应[J]. 地理科学进展, 2004,23(2):893-900.
[4]
赵新全, 陈世龙, 曹广民 , 等. 青藏高原高寒草甸生态系统与全球气候变化的相互作用机理研究[J]. 科技和产业, 2003,8(3):51-59.
[5]
彭岳林, 蔡晓布, 薛会英 . 退化高寒草原土壤微生物变化特性研究[J]. 西北农业学报2007, 16(4):112-115.
[6]
芦晓飞 . 西藏米拉山高寒草甸土壤微生物多样性研究[D]. 北京:中国农业科学院, 2009.
[7]
牛佳, 周小奇, 蒋娜 , 等. 若尔盖高寒湿地干湿土壤条件下微生物群落结构特征[J]. 生态学报, 2011,31(2):0474-0482.
[8]
张瑞益 . 北方主要草地类型土壤线虫群落结构及多样性的比较研究[D]. 长春:东北师范大学, 2015.
[9]
周娟, 郭卫华, 宗美娟 , 等. 不同植被下可培养细菌多样性[J]. 山东大学学报, 2006,41(6):161-167.
[10]
卢许波, 赵阳, 德吉央金 , 等. 拉萨扎叶巴沟瑞香狼毒根际土壤细菌的分离与鉴定[J]. 广东农业科学, 2016,43(10):95-99.
[11]
周阳薇, 刘正坪, 魏艳敏 , 等. 植物根际土壤细菌的分离与鉴定[J]. 中国农学通报, 2015,31(7):212-217.
[12]
谢汶芝, 罗明, 韩剑 , 等. 塔吉克斯坦哈特隆州土壤可培养细菌多样性分析[J]. 干旱区地理, 2016,39(1):144-153.
[13]
戚珊珊, 周礼红, 胡久平 , 等. 西藏多地区土壤可培养细菌的分离鉴定及多样性分析[J]. 西南农业学报, 2017,30(7):1629-1635.
[14]
吴斌, 姜珊珊, 张眉 , 等. 小麦黄花叶病毒病不同发生度土壤的微生物多样性研究[J]. 山东农业科学, 2017,49(12):44-49.
[15]
秦越 . 宁夏黄土高原马铃薯连作及间作栽培对土壤微生物遗传多样性的影响[D]. 银川:宁夏大学, 2014.
[16]
何建清, 张格杰, 岳海梅 . 珠穆朗玛峰自然保护区土壤放线菌的生态分布及拮抗性[J]. 安徽农业科学, 2010,38(32):18163-18165.
[17]
张栋, 张晓瑜, 汤辉 , 等. 河北平泉农田土壤放线菌物种多样性筛查[J]. 食品工业科技, 2014,35(8):197-201.
[18]
苏雪, 张辉, 冯克宽 . 榆中县北山干旱地区不同作物根际放线菌的分离与鉴定[J]. 西北师范大学学报, 2005,41(3):67-74.
[19]
周娟, 龙云川, 刘峙嵘 , 等. 贵州黄果树土壤放线菌的分离与纯化研究初报[J]. 贵州科学, 2016,34(5):5-7.
[20]
郑亚强, 陈斌, 宋培勇 , 等. 马铃薯与玉米间作体系根际土壤放线菌多样性及拮抗菌株的筛选[J]. 西北农业学报, 2016,25(6):912-920.
[21]
李永欣, 张栋, 张晓瑜 , 等. 土壤放线菌分离与16S rDNA系统发育分析[J]. 河北农业科学, 2014,43(3):75-80.
[22]
马爱爱 . 青藏高原不同生境土壤放线菌多样性及生理活性研究[D]. 兰州:兰州大学, 2012.

RIGHTS & PERMISSIONS

Copyright reserved © 2020. Chinese Agricultural Association. All articles published represent the opinions of the authors, and do not reflect the official policy of the Chinese Agricultural Association or the Editorial Board, unless this is clearly specified.
Share on Mendeley
PDF(1266 KB)

Collection(s)

GAOL 13: CLIMATE ACTION

Accesses

Citation

Detail

Sections
Recommended

/