Genetic Diversity Analysis of Peanut in Sichuan Based on SSR Markers

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Chinese Agricultural Science Bulletin ›› 2019, Vol. 35 ›› Issue (25) : 43-53. DOI: 10.11924/j.issn.1000-6850.casb18030136

Genetic Diversity Analysis of Peanut in Sichuan Based on SSR Markers

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The aim is to provide a basis for the molecular breeding of peanuts (Arachis hypogaea L.) and the construction of resource library. 57 accessions of peanut from various regions of Sichuan Province were selected as experimental materials, four agronomic traits and four quality traits of the materials were tested and identified, and 67 pairs of SSR markers were used to evaluate the materials, and associated with the phenotypic traits. The results revealed that among the 8 traits of peanut resources, 6 traits had greater variation and better diversity. 39 SSR primers were effective among the 67 SSR primers, and 53 polymorphic bands were amplified with an average of 1.36 loci per primer. The mean of Nei’s gene diversity was 0.2288, and Shannon’s information index was 0.3695. Cluster analysis was conducted with UPGMA. The 57 accessions were divided into two major groups at 0.5200 of genetic similarity coefficient and the maximum genetic distance was 0.51. There were several association markers with protein content, plant height, and sugar content. The research shows that the genetic diversity of peanuts in different regions of Sichuan is relatively abundant. The genetic relationships among resources are not related to their geographical distribution. The characteristic differences between local resources and the main cultivars are obvious, and their genetic relationships are far away.

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Sichuan; peanut; SSR markers; genetic diversi

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Genetic Diversity Analysis of Peanut in Sichuan Based on SSR Markers. Chinese Agricultural Science Bulletin. 2019, 35(25): 43-53 https://doi.org/10.11924/j.issn.1000-6850.casb18030136

References

[1]孙大容.花生育种学[M].北京:中国农业出版社.1998.12-14.
[2]Macedo S E. Moretzsohn M C. Leal-Bertioli S C M. et al. Development and characterization of highly polymorphic long TC repeat microsatellitemarkers for genetic analysis of peanut[J].BMC Reasarch Notes,2012(5):86.
[3]洪彦彬,陈小平,梁炫强.花生基因组资源的开发及应用[J].中国油料作物学报,2012,34(3):326-335.
[4]詹世雄.花生遗传多样性的SSR标记分析及核心种质构建方法的探索[D].汕头:汕头大学,2007.
[5]陈亮,郑宏宇,范旭红,等.大豆SSR指纹图谱身份证的研究进展与展望[J].大豆科技,2015(2):38-46.
[6]邓姗,褚云霞,黄志城,等.上海地区水稻已知品种SSR指纹图谱库构建[J].中国农学通报,2015(3):7-15.
[7]程雪妮,王颖,庞玉辉,等.小麦SSR和EST-SSR引物对无芒雀麦的通用性分析[J].植物科学学报,2014,32(1):27-33.
[8]李晓荣,张菁雯,赵燕妮,等.油菜品种及高油育种材料遗传多样性SSR分析[J].西北植物学报,2015,35(2):258-268.
[9]郭奕生,何德银.SSR分子标记在玉米品种真实性鉴定上的应用[J].广东农业科学,2014,41(12):12-15.
[10]郭爽,张素平,丘漫宇,等.樱桃番茄种质资源亲缘关系的InDel和SSR标记分析[J].热带作物学报,2014,35(8):1464-1468.
[11]吴则东,王茂芊,王华忠,等.利用SSR快速鉴定甜菜品种纯度和真实性的研究[J].中国农学通报,2014(31):214-218.
[12]林茂,李正强,郑治洪,等.贵州省花生地方品种的遗传多样性[J].作物学报,2012,38(8):1387-1396.
[13]崔顺立,刘立峰,陈焕英,等.河北省花生地方品种基于SSR标记的遗传多样性[J].中国农业科学,2009,42(9):3346-3353.
[14]卞能飞,孙东雷,沈一,等.基于SSR标记的江苏省花生地方品种遗传多样性分析[J].中国油料作物学报,2017,39(2):170-177.
[15]李东霞,石鹏,杨伟波,等.利用SSR 分子标记分析海南与国内外花生种质资源的遗传多样性[J].广东农业科学.2015,20:118-124.
[16]夏友霖,李加纳,廖伯寿,等.川渝地区花生品种遗传多样性分析[J].中国油料作物学报,2008,30(3):300-305.
[17]姜慧芳,任小平,陈玉宁,等.中国花生地方品种与育成品种的遗传多样性[J].西北植物学报,2011,31(8):1551-1559.
[18]姜慧芳,任小平,张晓杰,等.中国花生小核心种质与ICRISAT微核心种质的SSR遗传多样性比较[J].作物学报,2010,36(7):1084-1091.
[19]任小平,张晓杰,廖伯寿,等.ICRISAT花生微核心种质资源SSR标记遗传多样性分析[J].中国农业科学,2010,43(14):2848-2858.
[20]徐日荣,唐兆秀.福建省花生地方品种的遗传多样性研究[J].分子植物育种,2014,12(6):1235-1242.
[21]詹世雄,郑奕雄,刘冠明,等.基于SSR标记的花生品种遗传多样性分析[J].中国油料作物学报,2014,36(2):269-274.
[22]肖宇,陈剑洪,郭皗篧,等.福建省近年来审(认)定花生品种的遗传多样性分析[J].植物遗传资源学报,2015,16(4):728-733.
[23]胡晓辉,毛瑞喜,苗华荣,等.山东省46个花生品种SSR指纹图谱构建与遗传多样性分析[J].核农学报,2015,30(10):1925-1933.
[24]殷冬梅,王允,尚明照,等.花生优异种质的分子标记与遗传多样性分析[J].中国农业科学,2010,43(11):2220-2228.
[25]周金超,杨鑫雷,崔顺立.等.花生SSR标记与农艺性状的相关性[J].作物学报,2014,40(7):1197-1204.
[26]NaitoY, Suzuki S, wata Y, et al. Genetic diversity and relationship analysis of peanut germplasm using SSR markers[J].Breeding Science,2008,58 (3): 293-300.
[27]Ren X, Jiang H, YanZ, et al. Genetic diversityand population structure of the major peanut (ArachishyPogaeaL.) cultivars grown in China by SSR markers [J].PloS One,2014,9(2):e88091.
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