To assess the effects of biochar application on the conversion of soil phosphorus and the numbers of phosphate solubilizing bacteria (PSB) in acid red soil, a eight week PSB inoculation microcosm incubation experiment was performed using soil from tea plantation, and biochar adding treatment of 0%, 1% and 3% (w /w ) were conducted. The results showed that: the numbers of PSB decreased rapidly after inoculation, the application of biochar significantly increased the numbers of PSB in tea plantation, which was 10.5-15.25 times as much as that of control treatment. In addition, the application of biochar increased soil pH value,available P (Bray-P) content, and alkaline phosphatase enzyme activities. Correlation analysis showed that: there were positive correlations between numbers of PSB and organic matter content, total nitrogen, total phosphorus, pH value and alkaline phosphatase enzyme activities; and a negative correlation between numbers of PSB and acid phosphatase enzyme activities. In summary, compared with control treatment, the application of biochar increased the numbers of PSB and activated soil P in red soil tea plantation.
Key words
biochar; phosphate solubilizing bacteria; soil available P; alkaline phosphatase enzyme activities; red soil tea plantation
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References
1. G O Mendes, D L Zafra, N B Vassilev, I R Silva, J I R Jr. and M D Costa. 2014. Biochar EnhancesSAspergillus nigerSRock Phosphate Solubilization by Increasing Organic Acid Production and Alleviating Fluoride Toxicity [J]. Applied and Environmental Microbiology, 80 (10):3081-3085.
2. 鲁如坤,时正元,顾益初. 1995. 土壤积累态磷研究Ⅱ.磷肥的表观积累利用率 [J]. 土壤, 1995(6): 286-289.
3. 谢学俭,陈晶中,宋玉芝,汤莉莉.2007.磷肥施用量对稻麦轮作土壤中麦季磷素及氮素径流损失的影响[J]. 农业环境科学学报,26(6):2156-2161.
4. 王光华,赵英,周德瑞,杨谦. 2003. 解磷菌的研究现状与展望[J]. 生态环境学报,12(1):96-101.
5. M Jorquera, O Martínez, F Maruyama,P Marschner,M L Mora.Current and Future Biotechnological Applications of Bacterial Phytases and Phytase-Producing Bacteria[J].23(3): 182-191.
6.易艳梅.2007.细菌溶磷作用及其对磷矿粉重金属释放和小麦盐胁迫的缓解[D].南京：南京农业大学.
7. A Peix, E Lang, S Verbarg, C Spr?er,SR Rivas, I Santa-Regina, P F. Mateos,SE Martínez-Molina,SC Rodríguez-Barrueco. 2009. Acinetobacter strains IH9Sand OCI1, two rhizospheric phosphate solubilizing isolates able to promote plant growth, constitute a new genomovar ofSAcinetobacterS calcoaceticus[J]. Systematic and AppliedSMicrobiology, 32(5):334-
341.
8. P Gyaneshwar,SGN Kumar, LJ Parekh, P S Poole. 2002. Role of soil microorganisms in improving P nutrition of plants [J].Plant and Soil, 245(1): 83-93.
9. BJJ Lugtenberg, L Dekkers, G V. Bloemberg. 2001. Molecular determinants of rhizosphere colonization by Pseudomonas [J]. Annual Review of Phytopathology 39:461-490
10. V Mittal,O Singh,H Nayyar,J Kaur,R Tewari.2008. Stimulatory effect of phosphate-solubilizing fungal strains (Aspergillus awamori and Penicillium citrinum) on the yield of chickpea (Cicer arietinum L. cv. GPF2) [J]. Soil Biology Biochemistry,S40(3):718-727.
11. K Rmn,HH Janzen,ME Leggett. 1989. Microbially mediated increases in plant-available phosphorus [J]. Advances in Agronomy,S42:199-228.
12. B Koch,J Worm,LE Jensen,O H?jberg,O Nybroe.2001.Carbon Limitation Induces ?S-Dependent Gene Expression in Pseudomonas fluorescens in Soil[J]. Applied and Environmental Microbiolgy, 67(8):3363-3370.
13. E Marris.2006. Putting the carbon back: black is the new green [J].Nature,S442(7103):624-626.
14. K Heike. 2007. How does fire affect the nature and stability of soil organic nitrogen and carbon? A review [J].Biogeochemistry,S85(1):91-118.
15. J Lehmann,MC Rillig,J Thies,CA Masiello,WC Hockaday,2011.Biochar effects on soil biota -A review [J]. Soil Biology and Biochemistry, 43:1812-36.
16. 陈敏,杜相革. 2015. 生物炭对土壤特性及烟草产量和品质的影响[J].中国土壤与肥料,(1):80-83.
17. A Fox,W Kwapinski, BS Griffiths, A Schmalenberger.2014. The role of sulfur‐ and phosphorus‐mobilizing bacteria in biochar‐induced growth promotion of Lolium perenne [J]. FEMS Microbiology Ecology: 78-91.
18. 鲁如坤. 土壤农业化学分析方法[M]. 北京：中国农业 科技出版社,2000.
19. HZ Zhang, CR Chen, E M. Gray, S E. Boyd, H Yang, DK Zhang. 2016. Roles of biochar in improving phosphorus availability in soils: a phosphate adsorbent and a source of available phosphorus [J]. Geoderma, 276:1-6.
20. Zheng, B.-X. et al. Long-term nitrogen fertilization decreased the abundance of inorganic phosphate solubilizing bacteria in an alkaline soil. Sci. Rep. 7,42284; doi:10.1038/srep 42284(2017)
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