The paper aims to find out the primary and secondary relationship of climate factors’ influence on chemical components during flue-cured tobacco growth in Zunyi, and provide a theoretical basis for production layout of flue-cured tobacco with high quality. Based on major chemical components of tobacco and the climate data in tobacco-growing area of Zunyi during 2009-2013, the authors used the fuzzy mathematics membership function model to assess the climate adaptability of this area, in addition, the authors also used the grey correlation analysis to analyze the correlation degree between major climate factors and chemical components of tobacco leaves. The results showed that: the average climate feasibility index (CFI) in tobacco-growing area of Zunyi was 0.853, which accorded with growth condition of tobacco leaves with high quality, however, climate change was great in tobacco-growing area of Zunyi between the years, the variation coefficient of sunshine duration and rainfall during various growth periods among different years was especially large, resulted in poor stability of chemical components of tobacco leaves in different years. The results of grey correlation analysis showed that the climate factor, which was significantly correlated with total sugar and reducing sugar contents of tobacco in Zunyi, was sunshine duration at each growth period, and the major climate factor which influenced the nicotine and total nitrogen content in tobacco was the rainfall at the vigorous growth period.