Expression of β-catenin in Uterus of Artificially Induced Oestrous Sheep

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Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (20) : 124-127. DOI: 10.11924/j.issn.1000-6850.casb16070069

Expression of β-catenin in Uterus of Artificially Induced Oestrous Sheep

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Abstract

To investigate the changes in the expression of β-catenin in uterus of oestrous sheep which was artificially induced, expression changes of β-catenin on the 10th, 12th, 14th, 16th and 18thday after sheep oestrus were detected respectively by Real-Time PCR and semi-quantitative PCR. Real-time quantitative and semi-quantitative PCR results showed that: two detection methods could detect mRNA expression in sheep uterus in different periods of β-catenin, and in 10th to 18th days were shown to decrease after increasing trend. The transcriptional level of β-catenin on the 10th day was significantly higher than that of the other days (P<0.05). In addition, β-catenin mRNA expression on the 12th day reduced significantly (P<0.05), and then remained relatively constantly, but significantly increased on the 18th day (P<0.05). Real-time PCR and semi-quantitative PCR can be used to detect the relative expression level of sheep uterine tissue of β-catenin mRNA; during the 10th to 18th day after sheep oestrus, the transcriptional level of β-catenin presented a decrease-increase trend.

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sheep; β-catenin; uterus; Real-time PCR; Semi-quantitative PCR

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Expression of β-catenin in Uterus of Artificially Induced Oestrous Sheep. Chinese Agricultural Science Bulletin. 2017, 33(20): 124-127 https://doi.org/10.11924/j.issn.1000-6850.casb16070069

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