Analysis of Genetic Diversity and Identification of Maintainer Line for K-CMS Germplasm Resources in Wheat Using SRAP Markers

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Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (20) : 5-10. DOI: 10.11924/j.issn.1000-6850.casb16070044

Analysis of Genetic Diversity and Identification of Maintainer Line for K-CMS Germplasm Resources in Wheat Using SRAP Markers

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Abstract

To analyze the genetic background of the maintainer line for K-type cytoplasmic male sterility (CMS) in wheat, screen the excellent parents for breeding, and improve hybrid breeding efficiency in wheat, the genetic diversity of 30 maintainer lines for K-CMS germplasm resources of wheat were assessed using SRAP markers in this study. SRAP markers were used to amplify the polymorphic loci in the 30 lines, the genetic distances between cultivars were calculated by using Nei and Li methods. In 2 years field experiment, the average of phenotypic traits about plant height, panicle length, spikelet number, spike length, spike grass weight, grain number and grain weight per spike, length of flag leaf and so on were carried out in normal standard, using Euclidean distance to calculate the genetic distance among varieties, and comparative analysis was conducted. Analysis showed that 82 pairs of SRAP primers revealed polymorphism, the polymorphism rate was 68.3% , 208 polymorphic bands were obtained, the average of each pair of primers produced 3.68 polymorphic bands, showed high polymorphism. The results of cluster analysis based on molecular markers and pedigree analysis results were consistent. Taking the total average genetic distance as the scale, the results of cluster graph could be divided into 6 categories. Mantel detection showed that there was a very significant correlation between the genetic distance matrix of SRAP marker data and the expression pattern of the genetic distance matrix (r =0.8123,t =11.325>t 0.01). SRAP marker is an effective method to detect the genetic difference among cultivars, which can provide theoretical basis for the research of genetic difference of germplasm resources of K-CMS in wheat. Studies also confirm that the genetic difference between a backbone parent and the breed derived from it is generally small.

Key words

Wheat; Maintainer line for K-type cytoplasmic male sterility (CMS); Genetic diversity; SRAP; Agronomic traits

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Analysis of Genetic Diversity and Identification of Maintainer Line for K-CMS Germplasm Resources in Wheat Using SRAP Markers. Chinese Agricultural Science Bulletin. 2017, 33(20): 5-10 https://doi.org/10.11924/j.issn.1000-6850.casb16070044

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