Strawberry Plantlets Somaclonal Variation

Hu Panpan,Zhao Xia,Li Gang,Li Liangjie,Qin Bao,Peng Zhuo and Zhou Houcheng

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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (16) : 77-82. DOI: 10.11924/j.issn.1000-6850.casb15100124

Strawberry Plantlets Somaclonal Variation

  • Hu Panpan1,2, Zhao Xia1,2, Li Gang1,2, Li Liangjie1,2, Qin Bao2, Peng Zhuo2, Zhou Houcheng1,2
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Abstract

Variations are easily induced in tissue culture of plants. They significantly affect the genetic stability of plants, and limit the application of tissue culture technology in agriculture. Strawberryin vitroseedlings have been widely used in agricultural production. However, the variations often occur in tissue culture of plant, which seriously influence the agricultural production efficiency. Therefore, it is an urgent problem to effectively control the occurrence of the variations in vitrorapid propagation of strawberry and other plants. The main factors affecting the occurrence of the variations were analyzed. These factors included explant types, genotypes, the types and proportion of different hormones in the medium, the time and times of subculture. And the inherent genetic mechanism of the variations had chromosomal abnormalities, transposon activation, gene mutation and so on. In order to effectively control the variation occurrence in tissue culture of strawberry, it was suggested to select the young explants, optimize the culture medium formula, control the multiplication coefficient and the subculture times, and culture the tissues under proper light and temperature conditions. Meanwhile, using the methods of morphological observation, RAPD, SSR and so on to detect the variations in tissue culture seedlings could eliminate the variant seedlings and reduce the adverse effects of the variant plants to production. By summarizing the causes and the effective controlling ways of the variation of strawberry in vitro, it would provide valuable reference for the healthy development of plant virus-free tissue and rapid propagation of plants including strawberry. Moreover, the deep understanding of variation would provide a new way for the improvement and breeding of new varieties.

Key words

strawberry; somaclonal variation; rapid propagation; application

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Hu Panpan,Zhao Xia,Li Gang,Li Liangjie,Qin Bao,Peng Zhuo and Zhou Houcheng. Strawberry Plantlets Somaclonal Variation. Chinese Agricultural Science Bulletin. 2016, 32(16): 77-82 https://doi.org/10.11924/j.issn.1000-6850.casb15100124

References

[1] 雷家军,代汉萍,谭昌华,等.中国草莓属(Fragaria)植物的分类研究[J].园艺学报,2006,3(1):1-5.
[2] 朱丽君,张馨玉,袁云香,等.草莓组织培养的研究概述[J].辽宁农业科学,2014(6):56-58.
[3] 覃兰英,徐光霞,邓世秀.草莓茎尖离体培养大量繁殖研究初报[J].中国果树,1981,3:38-41.
[4] 司长征,蔡联军,李国强.红颜草莓组培脱毒壮苗快繁技术与应用[J].中国果菜,2013,4:6-9.
[5] 梁凤龙.几种草莓优良品种的组培快繁和脱毒苗生产技术研究[J].现代园艺,2013,10:23.
[6] 王晓云,张晓申,赵海红,等.草莓茎尖组培快繁技术研究[J].农业科技通讯,2014,12:124-125.
[7] 刘维,党聪,孟庆庆,等.草莓优良品种的组培快繁和脱毒苗生产技术研究[J].北京农业,2015(20):51-52.
[8] 张馨宇,张志宏,高秀岩,等.草莓微繁殖苗及其后代性状表观遗传变异研究[J].果树学报,2006,23(4):542-546.
[9] 赵月明,赵雁鸣,刘玉鲲,等.植物体细胞变异的防控及育种应用[J].植物学研究,2013,2:117-124.
[10] Bhojwani S S, Dantu P K. Plant tissue culture: an introductory text[M].London,Springer,2013:141-153.
[11] 李美林,张勇,朱丽君,等.草莓的组织培养研究进展[J].吉林农业,2013,8:14.
[12] 薛其勤,李美芹,吕金浮,等.不同基因型优质草莓组织培养快繁研究[J].北方园艺,2014(21):15-16.
[13] Kadhimi A A, Alhasnawi A N, Mohamad A, et al. Tissue culture and some of the factors affecting them and the micropropagation of strawberry[J]. Life Science Journal,2014,11(8):484-493.
[14] 王婷婷,蔡蕊,武慧,等.激素配比对草莓叶片不定芽分化的影响[J].北方园艺,2014(8):96-99.
[15] 李会珍,徐东进,陈登金,等.不同植物生长调节剂对脱毒红颊草莓组培快繁的影响[J].江苏农业科学,2013,41(20):43-45.
[16] 刘福平.植物体细胞无性系变异的遗传基础及主要影响因素[J].基因组学与应用生物学,2010,29(6):1142-1151.
[17] Guo L F, Xue F D, Guo J F, et al. Plant tissue culture: a recent progress and potential applications[J]. Agricultural Science & Technology,2014,15(12):2088-2095,2099.
[18] 金真,王康丽,梁佳惠,等.培养基和外源激素对‘章姬’草莓茎尖培养的影响研究[J].农学学报,2015,5(8):73-77.
[19] Nehra N S, Kartha K K, Stushnoff C, et al. The influence of plant growth regulator concentrations and callus age on somaclonal variation in callus culture regenerants of strawberry[J]. Plant Cell, Tissue and Organ Culture,1992,29(3):257-268.
[20] 冯建荣,樊新民,马兵刚,等.草莓愈伤组织诱导苗染色体行为变异的研究[J].石河子大学学报,2001,5(1):27-29.
[21] 单春华,邢如义.组织培养条件下草莓染色体数变异[J].农机化研究,2002,1:106-107.
[22] Zhang D, Wang Z H, Wang N N, et al. Tissue culture-induced heritable genomic variation in rice, and their phenotypic implications[J]. PLoS One,2014,9(5):e96879.
[23] Kumar S. Studies on plant regeneration and somaclonal variation in wheat[D]. Indian,Chaudhary Charan Singh University,2014.
[24] Amato F D, Bayliss M W. Cytogenetics of plant cell and tissue cultures and their regenerates[J]. Critical Reviews in Plant Sciences,1985,3(1):73-112.
[25] Mujib A, Banerjee S, Ghosh P D. Tissue cluture induced variability in some horticultural important rnamentals: chromosomal and molecular basis-a review[J]. Biotechnology,2013,12(6):213-224.
[26] Liu Z L, Han F P, Tan M, et al. Activation of a rice endogenous retrotransposon Tos17 in tissue culture is accompanied by cytosine demethylation and causes heritable alteration in methylation pattern of flanking genomic regions[J]. Theoretical and Applied Genetics,2004,109(1):200-209.
[27] Suoniemi A, Narvanto A, Schulman A H. The BARE-1 retrotransposon is transcribed in barley from an LTR promoteractive in transient assays[J]. Plant Molecular Biology,1996,31(2): 295-306.
[28] Azman A S, Mhiri C, Grandbastien M A, et al. Transposable elements and the detection of somaclonal variation in plant tissue culture: a review[J]. Malaysian Applied Biology,2014,43(1):1-12.
[29] Neelakandan A K, Wang K. Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications[J]. Plant Cell Report,2012,31(4):597-620.
[30] Zhang Z H. Epigenetic and genetic variation in micro-propagated strawberry plants[J]. Acta Horticulturae,2014,1049:63-66.
[31] Stelpflug S C, Eichten S R, Hermanson P J, et al. Consistent and heritable alterations of DNA methylation are induced by tissue culture in maize[J]. Genetics,2014,198(1):209-218.
[32] Wang X, Wu R, Lin X, et al. Tissue culture-induced genetic and epigenetic alterations in rice pure-lines, F1 hybrids and polyploids[J]. BioMed Central Plant Biology,2013,13:77.
[33] 韩柏明,赵恺,李贺,等.组织培养导致的草莓DNA甲基化变异[J].植物生理学通讯,2010,46(8):797-802.
[34] Mohamed A E. Somaclonal variation in micro-propagated strawberry detected at the molecular level[J]. International Journal of Agriculture & Biology,2007,9(3):721-725.
[35] Haque M S, Nath U K, Iqbal M S, et al. Assessment of field performance and genetic diversity analysis of tissue culture variants of strawberry[J]. Journal of Agricultural Technology,2015,11(1):107-125.
[36] Kinet J M, Parmentier A. The flowering behaviour of micropropagated strawberry plants cv. ‘Gorella’: the influence of the number of sub-cultures on the multiplication medium[J]. Acta Horticulturae,1989,265(48):327-334.
[37] Abdellatif K F, Hegazy A E, Aboshama H M, et al. Morphological and molecular characterization of somaclonal variations in tissue culture-derived banana plants[J]. Journal of Genetic Engineering and Biotechnology,2012,10(1):47-53.
[38] Bairu M W, Aremu A O, Staden J V. Somaclonal variation in plants: causes and detection methods[J]. Plant Growth Regulation,2011,63(2):147-173.
[39] 吴伟怀,郑肖兰.香蕉组培苗变异的原因、防止及其利用研究综述[J].中国南方果树,2007,36(4):31-34.
[40] 丰先红,李健,罗孝贵.植物组织培养中体细胞无性系变异研究[J].中国农学通报,2010,26(14):70-73.
[41] 温映红,杨明霞,王俊宇,等.生物技术在草莓遗传育种中的应用[J].农学学报,2014,4(8):88-91.
[42] Sales E K, Butardo N G. Molecular analysis of somaclonal variation in tissue culture derived bananas using MSAP and SSR markers[J]. International Journal of Biological,2014,8(6):615-622.
[43] Bose L K, Kar M, Bhattacharya B, et al. A highly efficient and improved protocol for exploitation of somaclonal variation for enhancing alien gene introgression in wide cross hybrid of Oryza sativa/Oryza brachyantha[J]. African Journal of Agricultural Research,2014,9(2):262-268.
[44] Karim R, Ahmed F, Krishna Roy U, et al. Varietal improvement of strawberry (Fragaria×ananassaDutch.) through somaclonal variation using in vitro techniques[J]. Journal of Agricultural Science and Technology,2015,17(4):977-986.
[45] Yaacob J S, Mahmad N, Mat Taha R, et al. Optimization of culture conditions (sucrose, pH, and photoperiod) for in vitro regeneration and early detection of somaclonal variation in ginger lime (Citrus assamensis)[J]. The Scientific World Journal,2014,2014:262710.
[46] Liu S M, Sykes S R, Clingeleffer P R. Improved in ovulo embryo culture for stenospermocarpic grapes (Vitis vinifera L.)[J]. Australia Journal of Agricultural Research,2003,54(9):869-876.
[47] 邹雪,肖乔露,文安东,等.通过体细胞无性系变异获得马铃薯优良新材料[J].园艺学报,2015,2(3):480-488.
[48] 张东旭,周增产,卜云龙,等.植物组织培养技术应用研究进展[J].北方园艺,2011(6):209-213.
[49] Rastogi J, Siddhant P B, Sharma B L. Somaclonal variation: a new dimension for sugarcane improvement[J]. GERF Bulletin of Biosciences,2015,6(1):5-10.
[50] 徐刚.植物组培技术在花卉领域应用广阔[J].中国花卉园艺,2009,4:12-13.
[51] Nwauzomal A B, Jaja E T. A review of somaclonal variation in plantain (Musa spp): mechanisms and applications[J]. Journal of Applied Biosciences,2013,67:5252-5260.
[52] 高清华,叶正文,张学英,等.草莓生物技术育种研究进展[J].分子植物育种,2006,4(3):123-129.
[53] Jamsheed S, Rasool S, Koul S, et al. Crop improvement through plant tissue culture[A]. Hakeem K R, Ahmad P, Ozturk M. Crop Improvement[C]. London, Springer,2013:123-148.
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