Analysis of Flax Stem Microflora Community Diversity by Denaturant Gradient Gel Electrophoresis

Zhou Xiaohang,Ge Jingping and Ping Wenxiang

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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (6) : 102-105. DOI: 10.11924/j.issn.1000-6850.casb15090066

Analysis of Flax Stem Microflora Community Diversity by Denaturant Gradient Gel Electrophoresis

  • Zhou Xiaohang1,2, Ge Jingping2, Ping Wenxiang2
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Abstract

Flax is an important economic crop in China. In order to improve the efficiency of retting, the authors used PCR-DGGE technique to study the flax stem microflora diversity, and optimized the best condition of this method. The results showed that, when PCR-DGGE was carried out using the gel recycling samples, the electrophoresis time was 200-220 min, modified gel gradient was 25%-55%, fixed time was about 12-15 min, dyeing time was 20 min, developing time was 5-10 min, the electrophoresis and separation results were good. The study clearly obtained the bacteria diversity in flax retting by optimizing the electrophoresis condition of DGGE. It could set a base for the use of PCR-DGGE to study the microflora community diversity of flax stem.

Key words

PCR-DGGE; flax stem; microflora community diversity

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Zhou Xiaohang,Ge Jingping and Ping Wenxiang. Analysis of Flax Stem Microflora Community Diversity by Denaturant Gradient Gel Electrophoresis. Chinese Agricultural Science Bulletin. 2016, 32(6): 102-105 https://doi.org/10.11924/j.issn.1000-6850.casb15090066

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