Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer

Cheng Longfei,Fu Qiuling,Chen Hongmei,Fu Guanghua,Shi Shaohua,Wan Chunhe and Huang Yu

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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (2) : 5-8. DOI: 10.11924/j.issn.1000-6850.casb15070112

Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer

  • Cheng Longfei, Fu Qiuling, Chen Hongmei, Fu Guanghua, Shi Shaohua, Wan Chunhe, Huang Yu
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Abstract

The objective of this test was to make a prokaryotic express of the bacteriophage RAP44 lysin gene of Riemerella anatipestifer. The gene of lyase was amplified by PCR using genomic DNA of bacteriophage RAP44 and inserted into pGEX-6P-1 vector. The constructed recombinant plasmid was transformed into Escherichia coli BL21(DE3) to express fusion protein, which was purified by Glutathione-Sepharose 4B. The results showed that expression plasmid pGEX-N was constructed successfully. The soluble recombinant protein about 37 kD was expressed after induced by IPTG. The purified fusion protein was generated in this study, which laid a foundation for the functional study of bacteriophage lysin of Riemerella anatipestifer.

Key words

Riemerella anatipestifer; bacteriophage; lysin; prokaryotic expression

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Cheng Longfei,Fu Qiuling,Chen Hongmei,Fu Guanghua,Shi Shaohua,Wan Chunhe and Huang Yu. Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer. Chinese Agricultural Science Bulletin. 2016, 32(2): 5-8 https://doi.org/10.11924/j.issn.1000-6850.casb15070112

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