Molecular Cloning and Sequence Analysis of the 1Dx Gene in Wheat Line 2004s-47

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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (21) : 115-122. DOI: 10.11924/j.issn.1000-6850.casb15030014

Molecular Cloning and Sequence Analysis of the 1Dx Gene in Wheat Line 2004s-47

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Abstract

Clarifying the sequence of 1Dx gene in 2004s-47 line would provide a theoretical basis for finding new types of high-quality HMW-GS and achieve wheat quality improvement and breeding. The high molecular weight glutenin subunits (HMW-GS) composition of 2004s-47 line was analyzed by SDS-PAGE. 1Dx subunit gene was cloned by PCR and sequence analysis was performed by DNAMAN. The results revealed that the gene sequence that cloned from 2004s-47 was 2514 bp and had typical x-type structure of wheat HMW-GS. And because the expressions of x- and y-type genes in the D genome of bread wheat were easier than that in the A and B genomes, together with the results of SDS-PAGE, the composition of 2004s-47 line was (Null， 7+ 8， ? + 10). Sequence alignments showed that the DNA sequence of the 1Dx？ gene from 2004s- 47 had the highest similarity to that of previously published 1Dx2.1t (AF480486) in Aegilops tauschii. The sequence was submitted to NCBI and sequence accession number: KP702118 was obtained. The identification of 1Dx gene sequence in 2004s-47 line provided a theoretical basis for the determination of whether 1Dx? was a new type of gene by prokaryotic expression, and for wheat quality improvement and breeding.

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HMW-GS; gene isolation; sequence analysis; 1Dx?; Aegilops tauschii

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Molecular Cloning and Sequence Analysis of the 1Dx Gene in Wheat Line 2004s-47. Chinese Agricultural Science Bulletin. 2015, 31(21): 115-122 https://doi.org/10.11924/j.issn.1000-6850.casb15030014

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