Resistance Identification and Influence Factor of Rice False Smut

Fu Rongtao,Wang Jian,Lu Daihua,Zhang Hong,Gong Xueshu,Chen Xuejuan,Ren Hongzhi and Mao Jianhui

PDF(1965 KB)
PDF(1965 KB)
Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (18) : 266-272. DOI: 10.11924/j.issn.1000-6850.casb15020105

Resistance Identification and Influence Factor of Rice False Smut

  • Fu Rongtao1,2, Wang Jian1,2, Lu Daihua1,2, Zhang Hong1,2, Gong Xueshu1,2,Chen Xuejuan1,2, Ren Hongzhi1,2, Mao Jianhui1,2
Author information +
History +

Abstract

Rice false smut is a kind of panicle disease, nowadays it has risen from a secondary disease to one of the main diseases of rice. To increase accuracy of resistance identification for rice false smut, this experiment conducted a comprehensive study, including inoculation methods, inocula, planting time, infection condition and environmental condition in the field and greenhouse. This study compared three inoculation methods and found that the best effect was the combination of injection and spraying method. Among four inocula, the mixture of hypha fragment and thin-wall conidia caused the most severe disease, whereas mycelium fragments and chlamydospores kept in room last year could not cause false smut. The treatments of sun-shade and moisturizing spray had a better disease effect after inoculated with pathogen in field or greenhouse conditions. The temperature of 25-30℃ and relative humidity above 85% were more favorable to infection and symptom appearance of the disease. In addition, the results showed that more stable identification effect could be obtained in the greenhouse than in field condition. In order to get a more effective and reliable assessment result, it suggested that the identification of disease resistance of rice should combine the artificial-natural resistance identification in field and artificial inoculation in greenhouse.

Key words

rice; rice false smut; resistance identification; inoculation method

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
Fu Rongtao,Wang Jian,Lu Daihua,Zhang Hong,Gong Xueshu,Chen Xuejuan,Ren Hongzhi and Mao Jianhui. Resistance Identification and Influence Factor of Rice False Smut. Chinese Agricultural Science Bulletin. 2015, 31(18): 266-272 https://doi.org/10.11924/j.issn.1000-6850.casb15020105

References

[1] Tanaka T, Ashizawa T, Sonoda R, et al. Villosiclava virens gen. nov., com. nov., teleomorph of Ustilaginoidea virens, the causal agent of rice false smut [J]. Mycotaxon, 2008(106):491-501.
[2] Dhindsa H S, Aulakh K S, Chahal S S. Incidence and assessment of losses due to false smut of rice in Punjab [J]. India phytopathology, 1991, 44(1):120-121.
[3] Sinha R K P, Sinha B B, Singh A P. Assessment of yield loss due to false smut disease of rice [J]. Journal of Applied Biology, 2003,13(1/2):35-37.
[4] Shan T J, Sun W B, Liu H, et al. Determination and analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice [J]. International Journal of Molecular Sciences, 2012(13):11275-11287.
[5] Awvhu R T. Fungitoxic effects of extracts from some west African plants [J]. Ann. Appl. Biol., 1989(115):451-453.
[6] Koiso Y, Li Y, Iwasaki S, et al. Ustiloxin, antimitotic cyclic peptides from false smut balls on rice panicles caused by Ustilaginoidea virens [J]. J. Antibiotics, 1994(47):765-773.
[7] Li Y, Koiso Y, Kobayashi H, et al. Ustiloxins, new antimitotic cyclic peptides: interaction with porcine brain tublin. Biochem. Pharmacol, 1995(49):1367-1372.
[8] Nakamura K, Izumiyama N. Lupinosis in mice caused by ustiloxin and a crude extract of fungal culture of Ustilaginoidea virens [J]. Biochemical pharmacology, 1995, 49(10): 1367-1372.
[9] Nakamura K I, Izumiyama N, Ohtsubo K I, et al. “Lupinosis”- like lesious in mice caused by ustiloxin, produced by Ustilaginoidea virens: a morphological study [J]. Natural Toxins, 1994, 2(1): 22-28.
[10] Rush M C, Shahjahan A K M, Jones J P, et al. Outbreak of false smut of rice in Louisiana [J]. Plant Disease, 2000, 84(1):100.
[11] Duhan J C, Jakhar S S. Prevalence and incidence of bunt and false smut in paddy (Oryza Sativa) seeds in Haryana [J]. Seed Research, 2000,28(2):181-185.
[12] Ahonshi M O, Adeoti A A. False smut on upland rice in eight rice producing locations of Edo state, Nigeria [J]. Journal of Sustainable Agriculture, 2002,20(3): 81-94.
[13] Barush B P, Senapoty D, Ali M S. False smut: a threat to rice growers in Assam [J]. India Journal of Mycology and Plant Pathology, 1992,22(3):274-277.
[14] 张君成,陈志谊,张炳欣,等.稻曲病的接种技术研究[J].植物病理学报, 2004,34(5):463-467.
[15] 杨秀娟,王舒婷,阮宏椿,等.水稻稻曲病室内人工接种技术[J].植物保护学报,2011, 38(5):395-400.
[16] 缪巧明,王永华.水稻品种对稻曲病的抗病性鉴定技术研究[J].西南农业学报,1994,7(2):67-70.
[17] Fu R T, Yin C C, Deng Q M, et al. Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus [J]. Tropical Plant Pathology,2014,39(3):203-209.
[18] Ashizawa T, Takahashi M, Arai M, et al. Rice false smut pathogen, Ustilaginoidea virens, invades through small gap at the apex of a rice spikelet before heading [J]. Journal of General Plant Pathology, 2012,78(4):255-259.
[19] Tang Y X, Jin J, Hu D W, et al. Elucidation of the infection process of Ustilaginoidea virens (teleomorph: Villosiclava virens) in rice spikelets [J]. Plant Pathology, 2013(62):1-8.
[20] Hu M L, Luo L X, Wang S, et al. Infection processes of Ustilaginoidea virens during artificial inoculation of rice panicles [J]. Eur J Plant Pathol, 2014(139):67-77.
[21] Li W L, Li L Y, Feng A P, et al. Rice false smut fungus, Ustilaginoidea virens, inhibits pollen germination and degrades the integuments of rice ovule [J]. American Journal plant Science, 2013,4: 2295-2304.
[22] Ashizawa T, Kataoka Y. Detection of Ustilaginoidea virens in rice panicles before and after heading in the field using nested-PCR technique with species-specific primers [J]. Japanese Journal of Phytopathology, 2005, 71(1):16-19.
[23] Fujita Y, Sonoda R, Yaegashi H. Inoculation with conidiospores of false smut fungus to rice [Oryza sativa] panicles at the booting stage [J]. Annals of the Phytopathological Society of Japan, 1989, 55(5):629-634.
[24] Wang S, Li M, Dong H, et al. Sporulation, Inoculation methods and pathogenicity of Ustilaginoidea albicans, the cause of white rice false smut in china [J]. J. Phytopathology, 2008, 156:755-757.
[25] 刘安国,何念杰,王金莲. 稻曲病[Ustilaginoidea virens (cooke) Takahashi]厚垣孢子发芽力的研究[J].江西农业大学学报,1989,40:29-34.
[26] 张宝棣,黎毓干,康必鉴,等.稻曲病生理学特性及厚垣孢子微观特征[J].云南农业大学学报,1995(1):35-39.
[27] 王伟刚,郑明,谢春甫,等.水稻稻曲病流行危害分析及防治对策[J].现代农业科学,2009, 16(3):163-165.
[28] Fu R T, Yin C C, Liu Y, et al. The influence of nutrient and environmental factors on mycelium growth and conidium of false smut Villosiclava virens [J]. African Journal of Microbiology Research, 2013,7(9):825-833.
[29] 杨健源,曾列先,陈深,等.中国稻曲病研究进展[J].广东农业科学, 2011,38(2):77-79.
[30] 杨洵.黔东南稻曲病的发生条件及预测预报与防治[J].贵州农业科学,2007,35(1):99-101.
Share on Mendeley
PDF(1965 KB)

Collection(s)

Rice

36

Accesses

0

Citation

Detail
Sections
Recommended

/

〈 〉