Biogas fertilizer is a kind of high-quality and total effects organic fertilizer. In order to understand the effect of different ratios of biogas fertilizer on cut lily growth, quality and yield, the author conducted experiments. The experiments were designed with same amount of nitrogen fertilizer, 2 levels of basic fertilizer and 3 levels of additional fertilizer, total 6 treatments with 3 replicates, all treatments followed randomized block arrangement. The variety of cut lily was‘Sorbonne’and biogas fertilizer was biogas residue and biogas slurry after fully rotten. The results showed that the cut lily’ s yield of B1A1 and B2A3 (CK) reached extremely significant level, B1A1 had the highest yield of 16 branches per m² the application of 1.06 kg/m² biogas residue as basic fertilizer and 2.7 kg/m² biogas slurry as additional fertilizer. Biogas fertilizer had obvious effects on aboveground part dry weight, plant height, flag leaf width, flower number, flower size, the rate of cut flower and so on, and it could improve plant characteristics and quality of cut lily. B1A1 had the best biological yield, plant height, flower number of per plant and the rate of cut flower. Biological yield and cut lily yield were B1＞ B2 under different ratios of biogas residue as basic fertilizer, and biological yield and cut lily yield had the highest amount under biogas residue treatment. The cut lily plant height and the length of the first bud were A1＞A2＞A3. With the application amount of biogas slurry increased, the cut lily plant height and the length of the first bud increased. The results could provide technical guidance for efficient production of cut lily.