Establishment of A Highly Efficient Plant Regeneration System of P.×Liaoningensis

Zhao Xinwen,Peng Rusheng and Zhao Jimei

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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (10) : 13-16. DOI: 10.11924/j.issn.1000-6850.casb14110115

Establishment of A Highly Efficient Plant Regeneration System of P.×Liaoningensis

  • Zhao Xinwen, Peng Rusheng, Zhao Jimei
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Abstract

In order to enhance its resistance to cold, disease and pests and enlarge its growing area, a highly efficient plant regeneration system with 100% shoot regeneration and rooting rate from leaf explants of P.×Liaoningensis in vitro was established by comparing different concentrations of BA and NAA on MS medium, and it was concluded that the best medium for shoot regeneration was MSBA 0.3 mg/L NAA 0.05 mg/L, and the best medium for rooting was 1/2MS IBA 0.3 mg/L. The results of this study will contribute to the improvement of the resistance of poplar and the study of the molecular breeding.

Key words

P.×Liaoningensis; leaves; adventitious bud; plant regeneration

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Zhao Xinwen,Peng Rusheng and Zhao Jimei. Establishment of A Highly Efficient Plant Regeneration System of P.×Liaoningensis. Chinese Agricultural Science Bulletin. 2015, 31(10): 13-16 https://doi.org/10.11924/j.issn.1000-6850.casb14110115

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