Study on in Vitro Efficient Regeneration System of Apple Seedling Clone from ‘Megumi’

Hao Hongmei,Tian Yi,Zhang Liyi,Kang Guodong,Zhang Caixia and Cong Peihua

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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (1) : 83-87. DOI: 10.11924/j.issn.1000-6850.2014-2119


Study on in Vitro Efficient Regeneration System of Apple Seedling Clone from ‘Megumi’

  • Hao Hongmei1, Tian Yi1,2, Zhang Liyi1,2, Kang Guodong1,2, Zhang Caixia1,2, Cong Peihua1,2
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Abstract

The genetic transformation mediated by Agrabacterium tumefaciens in apple is a vital method in identification functions of genes and cultivation new germplasm. Apple is highly heterozygous due to its self-incompatibility. In order to obtain a genotype with low heterozygous and high regeneration capacity, seedlings from ‘Megumi’ that is self-compatibility are studied in this experiment. The author selected soft cuttings from 10 seedlings and cultivated them in vitro several months. The results showed that HZ-5 exhibited higher proliferative capacity than others. Further, the main factors affecting the regeneration of adventitious buds from leaf explants were studied. The results showed that the appropriate medium was MS 2.0 mg/LTDZ 0.5 mg/LNAA 30 g/L sucrose 2.6 g/LGelzan, and the optimal dark treatment period was 14-21 d；When grown to 3 cm, the adventitious buds were transferred into rooting medium 1/2MS 0.3 mg/L IBA 0.2 mg/LIAA 30 g/Lsucrose 6.5 g/L agar, and the rooting rate was 100%.

Key words

apple; seedling; adventitious buds; regeneration

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Hao Hongmei,Tian Yi,Zhang Liyi,Kang Guodong,Zhang Caixia and Cong Peihua.
Study on in Vitro Efficient Regeneration System of Apple Seedling Clone from ‘Megumi’. Chinese Agricultural Science Bulletin. 2015, 31(1): 83-87 https://doi.org/10.11924/j.issn.1000-6850.2014-2119

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