Tillering is an important agronomic trait which influences rice panicle number and yield, and meteorological conditions have great influence on tillering stage. To study effects of tillering stage low temperature on super hybrid early rice tillering, this paper uses medium super hybrid early rice combined‘Ganxin 203’as the material, artificial climate box as test equipment, and adopts manual control method to have the stress test of the double factors (i.e. temperature and duration) at the tillering beginning stage. With the aid of methods such as statistical analysis, multiple comparison, cluster analysis and so on, the paper analyzes the differences between total spike number and effective panicle number, and their relationship with temperature and low temperature days. The results showed that, total spike number and effective panicle number reduce with the decrease of temperature, the differences of total spike number and effective panicle number caused by temperature difference are extremely significant, and temperatures lower than 19℃at the tillering stage do great harm to tillering; while total spike number and effective panicle number reduce with the increase of low temperature days, but the differences were not obvious, and the adverse effects of short-time cold weather could not be ignored.